Digital Commons Network^™

The Use Of Heavy Solvents For Separating Seed From Soil, Edward D. Carter, Stephen Challis, Ian G. Ridgway

IGC Proceedings (1977-2023)

Problems related to separating seeds from soil in ecological studies involving seed-seedling dynamics of annual pastures are discussed. The special roles of non-flammable chlorinated solvents as flotation agents in separating seed from soil are described. Studies showed that there was a slight, but significant (P < 0.05), overall effect of these chemicals on germination of seed of six species and cultivars: however, perchloroethylene appears to have no effect on germination.

Antioxidant Therapy Reverses Hepatotoxicity Induced By Microcystin-Lr In A Cellular Model Of Metabolic Dysfunction-Associated Steatotic Liver Disease (Masld), Apurva Lad, Jason Kindle, Prajwal Hegde, Gabriel G. Kleer, Andrew L. Kleinhenz, Johnna A. Birbeck, Judy Westrick, Nicholas J. Peraino, Terry D. Hinds, Neeraja Purandare, Andrew M. Fribley, Steven T. Haller, David J. Kennedy

Markey Cancer Center Faculty Publications

Microcystin-LR (MC-LR) is a potent hepatotoxin that has been shown to cause liver damage even at doses lower than the established Low Observable Adverse Effect Level (LOAEL) of 200 μg/kg in animal models. We have previously observed that low-dose exposure to MC-LR in animals with diet-induced Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) and subsequent treatment with antioxidants like N-acetylcysteine (NAC) and the Na⁺/K⁺ ATPase-Src kinase inhibitor pNaKtide significantly alleviated hepatic infiltration of immune cells, downregulated markers of inflammation and hepatotoxicity, increased the breakdown of the toxin molecule, and restored phase I and II drug metabolism pathways, including …

A Conserved Ethylene-Triggered Cell Death Mechanism May Underlie Hollow Stem Formation Across Plant Species, Mengxiao Yan, Weijuan Fan, Yinghui Meng, Jiamin Zhao, Wei Yang, Ziyin Xu, Yusen Gao, Haiyan Zhuang, Wuyu Zhou, Yuqin Wang, Qingjun Huang, Ling Yuan, Hongxia Wang, Jun Yang

Plant and Soil Sciences Faculty Publications

Hollow stems have independently evolved multiple times across the plant kingdom and play crucial roles in plant development and various environmental adaptations. However, the mechanisms underlying stem hollowness remain poorly understood. Water spinach (Ipomoea aquatica) is one of the few hollow-stemmed plants in the Convolvulaceae family (eudicot: asterid), and its hollow stems are essential for thriving in aquatic environments. Using histochemical staining and transcriptome analysis, we found that programmed cell death (PCD) is involved in cavity formation at water spinach shoot tips. Single-cell and spatial transcriptome analyses further revealed that ethylene and reactive oxygen species (ROS) likely drive …

The Impact Of Food Insecurity And Associated Variables On Colon Cancer In Kentucky: An Ecologic Study, Claire E. Lynch

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Objective: Given colorectal cancer incidence rates are increasing, especially in socioeconomic disadvantaged areas, this study aims to determine if there is an association between specific socioeconomic variables, such as food insecurity, and colon cancer at the county-level in Kentucky.

Methods: Colorectal cancer age-adjusted incidence rates at the county-level were collected from the Kentucky Cancer Registry. Conty-level data was collected from County Health Ranking, The U.S Census Bureau, National Institute on Minority Health and Health Disparities and the Behavioral Risk Factor Surveillance System from 2019 to 2023. R was used to run statical analyses such as a linear regression and a …

Environmental Predictors Of Human Lyme Disease Incidence Across Appalachian Counties In Kentucky, Virginia, And West Virginia, 2000-2019, Karlie D. Rennison

Theses and Dissertations--Epidemiology and Biostatistics

This ecological study examined county-level environmental, landscape, and host-related predictors of Lyme disease incidence across Kentucky, Virginia, and West Virginia from 2000-2019. Lyme disease is an emerging public health concern in the Appalachian region, where geographic expansion and shifting ecological conditions may influence disease risk.

Lyme disease case rates (per 10,000 population) were analyzed in relation to climate variables, land cover metrics, host density measures, and rurality using publicly available data sources. Descriptive and regression analyses were conducted to evaluate associations between candidate predictors and disease incidence.

Substantial geographic variation in Lyme disease incidence was observed, with higher case rates …

Geospatial Analysis Of Breast Cancer Incidence, Mortality, And Access To Screening In Rural And Appalachian Virginia, Jennifer Osher, Caleb Brown, Phillip Nichols, Tariq Ayubi, Jeremy Powers

Journal of Appalachian Health

Introduction: Breast cancer is the most common malignancy among women in the United States (U.S.), despite recent survival rates improving nationally. However, rural and Appalachian regions have experienced slower mortality declines. These communities face unique health care-related challenges that may contribute to observed disparities.

Purpose: The purpose of this study is to assess differences in breast cancer incidence, mortality, and mortality-to-incidence ratio (MIR) between Appalachian and non-Appalachian counties, as well as rural and non-rural counties in Virginia (VA). Furthermore, we aim to evaluate whether availability of mammography is associated with these differences.

Methods: County-level breast cancer incidence and mortality data …

Migratory Songbirds As Potential Ectozoochorous Protist Dispersal Vectors, Silas E. Fischer, Joy J. Jackson, Elise C. Hoffman, Henry M. Streby, Trisha L. Spanbauer

Earth and Environmental Sciences Faculty Publications

Protist biogeography, speciation, and systematics continue to generate debate and inquiry because protist distributions and dispersal remain poorly resolved. Identifying potential vectors and basibionts for epibiont protists would contribute to our limited understanding of their ecology. Migratory animals seasonally link disparate landscapes, incidentally transporting other organisms in the process. Waterbirds are known microbe dispersers, but evidence for other avian groups is limited-such as taxa that often migrate longer distances. We asked whether terrestrial songbirds (Passeriformes) host diatoms (Bacillariophyta) by sampling tail plumage of four thrush species (Hermit Thrush Catharus guttatus, Swainson's Thrush C. ustulatus, Wood Thrush Hylocichla mustelina, American Robin …

The Kentucky Microbiome Dataset, Rachel L. Washburn, Douglas C. Curl

Research Data--KGS

The Kentucky Microbiome Dataset is a continuously updated geolocated dataset which describes the distribution and diversity of microorganisms across the Commonwealth of Kentucky. Each geolocated point represents a location reported in the peer-reviewed literature where microorganisms were sampled from soil, water, geologic substrates, or clinical settings. Environmental studies include only non-host-associated, non-clinical microorganisms sampled from natural environments. Clinical studies are mapped separately to highlight locations where investigations into host-associated or human health-related microbes have occurred.

For each location, associated metadata include study information, taxonomic classifications, sample type, sampling method, latitude and longitude, sampling date, publication reference, and a direct link …

Environmental Related Variation In A Natural Grassland Of Rio Grande Do Sul, Brazil, V De P. Pillar, A.V A. Jacques, I I. Boldrini

IGC Proceedings (1977-2023)

Vegetation ecology studies help research efforts to improve natural grassland productivity by laying foundations for improvement techniques directed to benefit desired plant com­munities. Experiments reveal knowledge about the environ­mental conditions responsible for community structure and productivity, while surveys are a fast and cheap means for exploratory studies. In either case, data interpretation are helped by multivariate methods described by different authors, e.g., Orloci and Kenkel (1985). Our work is aimed to identify the environmental factors most responsible for vegetation variation in natural grasslands, locally known as « campos », and to formulate hypotheses about the effects of fertilization and grazing …

Heartache In The Heartland: Unraveling The Social Roots Of Deaths Of Despair In Kentucky, Lyndsey K. Blair, Madeline M. Tomlinson, Michele Abee-Biskis, Jessica Hume, Gabri Warren

Journal of Appalachian Health

Introduction: Deaths of despair (DoD), encompassing suicides, drug overdoses, and alcohol-related liver diseases, have emerged as a critical public health crisis in the United States, with their rise particularly pronounced from 1995 to 2013 and exacerbated by the COVID-19 pandemic. Kentucky, grappling with high rates of substance use disorder, poor mental health, and economic hardship, is at the forefront of this issue, particularly in its rural and Appalachian regions.

Purpose: This study explores the social determinants contributing to DoD in Kentucky, focusing on economic and social factors that influence rising rates of suicide, drug overdose, and alcohol-related liver disease. The …

Seasonal Divergence In Reproductive Timing On The Verge Of Spring, Devraj Singh, Adam M. Fudickar, Ellen D. Ketterson

Biology Faculty Publications

Every year as spring approaches and day length increases, many birds begin to reproduce, an annual expression of seasonal phenology that requires physiological preparation. In species distributed over a broad geographic range, populations that breed at higher latitudes are often migratory and delay reproduction until later in the year as compared to those breeding at lower latitudes. Dark-eyed Juncos serve as an excellent model for understanding the timing mechanisms regulating population-level variation in seasonal reproductive responses. We compared two seasonally sympatric dark-eyed junco populations in early spring. One migrates (Junco hyemalis hyemalis) and breeds in Alaska and Canada, while the …

Environmental And Behavioral Determinants Of Congenital Defects: An Ecologic Study In Kentucky, Amelia G. Floyd

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Congenital defects are a significant contributor to infant morbidity and mortality. The multifactorial etiology of such defects presents complex challenges for public health. This ecologic study investigates the association between environmental and behavioral exposures and congenital defects rates at the county level in Kentucky from 2010 to 2019. The Kentucky Department for Public Health, Office of Vital Statistics, provided all relevant defect data. County-level exposure data was collected from multiple sources.

Descriptive and geospatial analyses revealed substantial geographic variability, with notable high defect rates in northeastern Kentucky. Negative binomial regression models identified behavioral and socioeconomic factors to be the strongest …

Influence Of Different Breeds Of Pigs To The Natural Succession, H D. Matthes, A.C D. Micklich, G Dietl, H Mohring, W Jentsch

IGC Proceedings (1977-2023)

The aim of this study was to find out the effect of pigs on the natural succession. We used 4 different breeds of pigs for our investigations. Because of the grazing behaviour and their rooting activity the total number of plants increased up to 30%. But the vegetation quickly returned to conditions prior to rooting, if the rooting activity fails to happen. Rooting activity of pigs on floodplain soil and following grazing in the next spring after rooting permits seeds of different species of plants to germinate and develop in the plant free areas. These plants often have little chance …

Some Ecological Aspects Of Semi-Natural And Temporary Grasslands, N Gaborcik, L Ondrasek, J Cunderlik, J Tomaskin, T Kizek

IGC Proceedings (1977-2023)

Some ecological aspects were studied in semi-natural and temporary grasslands at different levels of fertilizer application. The research was carried out in the upland region of central Slovakia at the altitude of 460 m in Banská Bystrica. At zero fertilizer application, soil CO2 production and Cox content were higher in the temporary grassland. Atmospheric N fixation was also higher in the temporary grassland. However, root accumulation, microbial biomass content and R : S ratio were lower in the temporary grassland. By comparison with the semi-natural grassland, leaf area index (LAI), net assimilation rate (NAR) and crop growth rate (CGR) were …

Study Of The Diversity Of Soil Animal Community In The Songnen Plain Grassland Of China, Yin Xiuqin, Zhu Tingcheng, Zhong Weiyan, Peng Wu Kui

IGC Proceedings (1977-2023)

This paper makes a diversity study of 8 habitats of soil animal communities in Songnen plain grassland. The research shows that the better the habitat condition is, the higher the diversity index of soil animals, and the worse the habitat condition is the lower the diversity index of soil animals. The diversity of soil animal communities has a positive relationship with soil organic matter, total nitrogen, and has a negative relationship with pH value. It has no obvious relationship with soil water content and total phosphorus. The diversity of soil animal communities decreases with increasing depth of soil layer and …

Clipping Intensity Impacts On Root Mass Variation Of Cool Season Grasses, D L. Stetson, W M. Sullivan

IGC Proceedings (1977-2023)

Seasonal changes in grass root and mat weight density were documented under different cutting managements. Kentucky bluegrass (Poa pratensis L.) and tall fescue (Festuca arundinacea Schreb.) received one annual cut, twice yearly forage harvest, and mown turf treatments. Orchardgrass (Dactylis glomerata L.) received one annual and twice yearly cutting treatments. Soil cores were collected to a 15 cm depth and divided into 7.5 cm increments. Roots and mat were recovered, washed, dried and weighed. Differences in years, seasons, species, treatments, and soil depth significantly influenced total root weight density and fine root density. With all species and …

Effects Of Nitrogen On The Growth Of Hyparrhenia Diplandra, J Nkandza

IGC Proceedings (1977-2023)

The nitrogen effects on Hyparrhenia diplandra were studied. Plants were grown on nutrient solutions of variable concentrations in nitrogen. The supplied nitrogen increased tillering, leaf production, lamina area, total dry matter accumulation and total nitrogen accumulation in lamina. A detailed examination showed that the effect on tillering was the main cause of the differences observed in dry weights between different treatments. The nitrogen supply elongated significantly the lamina and nitrogen deficiency increased its thickness; but this was not enough to compensate the loss of weight in lower nitrogen treatment. The lamina area as well as nitrogen accumulation in the lamina …

Movement Of Allelopathic Compound Coumarin From Plant Residue Of Sweet Vernalgrass (Anthoxanthum Odoratum L.) To Soil, Yoshito Yamamoto

IGC Proceedings (1977-2023)

This study investigated the movement of coumarin, an allelopathic compound, from the plant residue of sweet vernalgrass (Anthoxanthum odoratum L.) to soil, as well as the dynamics of coumarin in soil. The level of coumarin dissolved from sweet vernalgrass plant residue in both Andosols and Cambisols, which were watered every day, peaked on the 5th day after the beginning of watering, and fell gradually with each additional of day. Specifically, the coumarin content in Cambisols on the 5th day was 14.2 ppm, which is 4 times the coumarin level found in Andosols. The recovery percentage of coumarin extracted with …

Colorectal Cancer Survival Disparities In The Five Regions Of Georgia, Meng-Han Tsai, Daramola N. Cabral, Caitlyn Grunert, Justin X. Moore

Markey Cancer Center Faculty Publications

Background/objective

The objective of this study was to examine 5-year colorectal cancer survival rates. We also determined whether demographics, tumor characteristics, and treatment modality were associated with 5-year CRC survival in the Clayton, West Central, East Central, Southeast, and Northeast Georgia regions because the significant higher CRC mortality rates in these regions in comparison to the overall rates in the State of Georgia.

Methods

We conducted a retrospective cohort analysis using data from the 1975–2016 Surveillance, Epidemiology, and End Results program aggregated CRC patients to these five regions. Five-year CRC survival was calculated and stratified by the five regions of …

Considerations And Applications Of Environmental Dna In Biodiversity Studies, Sarah Tomke

Theses and Dissertations--Forestry and Natural Resources

Environmental DNA (eDNA) sampling—the detection of species through genetic material shed into the environment—has become an efficient and reliable method for biodiversity assessments. The method has grown in popularity due to its non-invasive and non-destructive nature, and has revolutionized monitoring efforts for rare, elusive, and cryptic species for which traditional sampling approaches are often costly and ineffective. Hundreds of studies have utilized this technique to study species occupancy, delineate distributions, and assess relative community composition. Although eDNA sampling is an effective strategy for many conservation and ecological studies, recently, light has been shed on some of its potential drawbacks and …

Generating Climate Resilience Or Intensifying Vulnerabilities? A Feminist Poltitical Ecology Analysis Of Climate Resilient Agriculutre Projects In Sri Lanka’S Dry Zone, Nethmi Sachithma Perera Bathige

Theses and Dissertations--Geography

Conserving water for irrigation of crops has become a concern in the dry zone of Sri Lanka due to changes in climate patterns. In response, the United Nations Development Program (UNDP) has developed climate-resilient water management projects designed to build climate-resilience and water management practices among farmers. One such program called ‘Wew Gam Pubuduwa’ disseminates climate smart home gardening techniques, where women farmers receive sprinkler and drip irrigation systems, seeds and fertilizers, as well as training on water management methods. This study draws on a feminist political ecology framework to analyze the experiences of women home gardeners who …

Evaluating Functional Diversity As Potential Early-Warning Indicator Of Rangeland Degradation, L.-M. Schwarz, C. P. Carmona, M. C. Bilton, F. Munyebvu-Chambara, K. Behn, A. Linstädter

IGC Proceedings (1977-2023)

Droughts and overgrazing play a crucial role in the degradation of semi-arid rangelands. This is evident in the loss of palatable long-lived grass species and bush encroachment. Early warning indicators are needed to mitigate long-term degradation and decline in essential forage provision. Functional diversity provides valuable information on ecosystem health. However, functional diversity indices have not yet been tested regarding their applicability as early warning indicators, revealing non-linear threshold behaviour. We therefore examined the following questions: (1) How do functional diversity indices respond to grazing pressure? (2) Does land tenure affect the relationship between functional diversity and grazing pressure? (3) …

The Appalachian Gap In Preventable Hospitalizations: Are We Seeing Any Progress?, Rachel Hogg-Graham, Juan Lang, Teresa M. Waters

Journal of Appalachian Health

Introduction: Previous studies have documented geographic variation in preventable hospitalizations between rural and urban areas, but much less is known about preventable hospitalization patterns between heterogeneous rural areas. Unique challenges related to access of care and poverty may put the rural Appalachian Region at risk for higher rates of preventable hospitalizations.

Purpose: This study examines whether within-rural differences in Kentucky’s preventable hospitalization rates exist and how these differences may be changing over time.

Methods: Longitudinal and geographic trends in county-level preventable hospitalization rates were examined using Kentucky hospital discharge data from 2016 to 2019. Regression models were run to determine …

Variation Of Ldmc And Sla Relationship Between Growth Forms In Natural Grasslands, J. Viégas, P. Cruz, J. P. Theau, C. Jouany, P. Ansquer, R. Al Haj Khaled, O. Therond, M. Duru

IGC Proceedings (1977-2023)

In agro-ecological studies, there is a growing interest in measuring both leaf dry matter content (LDMC) and specific leaf area (SLA). This interest lies on the fact that leaf traits are linked to gradients of environmental factors and ecosystem functions. Working with three contrasting wild species, Garnier et al. (2001) proposed a model linking these two traits. The model shows a relatively simple non linear and negative correlation between LDMC and SLA. Nevertheless, none of the species used to build the model were grasses (GRA) or forb rosettes (ROS = i.e. dicotyledonous with large entire leaves and absence of …

A Study In Cuba Of The Biology, Ecology And Agroecological Management Of Heteropsylla Cubana Crawford In Leucaena Leucocephala (Lam.) De Wit, N. Valenciaga, M. Felicia Díaz, T. E. Ruíz, M. Fernández, C. Mora

IGC Proceedings (1977-2023)

As a consequence of the increase in Leucaena leucocephala areas to counter the shortage of feed in Cuban cattle production, there is a risk of the development of Heteropsylla cubana Crawford (Hemiptera: Psyllidae) as a pest in silvopastoral systems with this legume. This psyllid is known to be the main phytophagous pest (Valenciaga, 2003), which produces damage in 95% of the apical region of branches. Since information on the identification and biology of a pest species is a necessary prerequisite for its management, a taxonomic, biological and ecological study was conducted to define Heteropsylla behaviour in Cuban conditions and elaborate …

County Incidence And Geospatial Trends Of Early-Onset Hypertensive Disorders Of Pregnancy In Kentucky, 2008-2017, Courtney J. Walker, Anna Kucharska-Newton, Steven R. Browning, W. Jay Christian

UK CARES Faculty Publications

Background Early‑onset hypertensive disorders of pregnancy (eHDP) are associated with more severe maternal and infant outcomes than later‑onset disease. However, little has been done to evaluate population‑level trends. Therefore, in this paper, we seek to address this understudied area by describing the geospatial and temporal patterns of county‑level incidence of eHDP and assessing county‑level demographics that may be associated with an increased incidence of eHDP.

Methods Employing Kentucky certificates of live and stillbirth from 2008–2017, this ecological study detected county‑level clusters of early‑onset hypertensive disorders of pregnancy using SaTScan, calculated average annual percent change (AAPC) with a join point analysis, …

Factors Associated With Sun Protection Behaviors Among Childhood Cancer Survivors, Melissa Fluehr, Gary Kwok, Jerod L. Stapleton, Margaret Masterson, Katie A. Devine

UK CARES Faculty Publications

Background: Childhood cancer survivors (CCS) are at increased risk of developing skin cancer. Engaging in sun-protective behaviors may ameliorate that risk, but prior work shows that survivors engage in suboptimal levels of sun-protective behaviors. Guided by the Health Belief Model (HBM), this study evaluated factors associated with sun-protective behavior among CCS.

Methods: This is a secondary analysis of a survey study of 94 adult survivors of childhood cancer recruited from a long-term follow-up clinic. Participants reported their sun protection habits, skin type/ sensitivity, barriers to sun protection, and perceived severity and susceptibility of getting skin cancer. Descriptive statistics were used …

Effects Of Landscape Structure On Plants Species Richness In Small Grassland Remnants In Two Different Landscapes, S. A. O. Cousins, O. Eriksson

IGC Proceedings (1977-2023)

There is an increasing interest in using the landscape as the operational scale in many ecological studies. Current species richness in the landscape may be explained by past land use, and habitats may harbour species favoured by an environment that no longer exists. In this study we have included both a landscape scale and a temporal scale. The objective was to explain species pattern and the effect of isolation, habitat size and surrounding land use, and past land use change, on small grassland remnants in rural landscapes.

Rapid Scoping Review Of The Epidemiological Evidence For Mercury Exposure And Prevalence Of Alzheimer’S Disease, Rebecca Mattingly

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

This rapid scoping review examines the current body of epidemiologic research evaluating the potential linkage between environmental exposure to mercury and Alzheimer's disease (AD). Alzheimer’s disease accounts for 60-80% of all neurodegenerative disease in the United States and was the seventh leading cause of death in Kentucky in 2020. The exact etiology of AD needs further investigation; however, environmental factors such as pesticides, arsenic, cadmium, lead, and mercury have been correlated with development of AD lesions, cognitive decline, and AD. Mercury is a toxic metal that can be found in air, water, and soil, both from natural and human-made sources. …

Roadway Density And Lung Cancer Histology In Kentucky, Mahala Pettus

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

The purpose of this article was to examine the relationship between a proxy measure of exposure to automotive exhaust – roadway density – and risk for adenocarcinoma lung cancer. Using data from the Kentucky Cancer Registry and the Kentucky Geoportal Data Clearinghouse, we conducted a cross-sectional study of the distribution of adult lung cancer cases by histological type across the state of Kentucky. The north-central region of Kentucky has a higher prevalence of adenocarcinomas and greater roadway density. Among adenocarcinoma cases in Kentucky, there are more non-smokers than other types of tobacco use. As smoking rates decrease in Kentucky, and …