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Root Chemistry Effects On Soil Organic Matter Fractions Persist In Topsoil And Subsoil Of A Well-Drained Alfisol, Brian Rinehart, Justin Allen, Joeri Kaal, Dave Mcnear, Hanna Poffenbarger

Plant and Soil Sciences Faculty Publications

Subsoils have been identified as a potential target for building soil C stocks, given the older age and slower cycling of organic matter at depth. Plant roots deliver C deep into the soil profile, but there is limited experimental evidence as to which root traits are most effective at increasing subsoil C. To that end, we conducted a 13C natural abundance study to trace C from roots of differing quality into organic matter fractions in a shallow and deep soil layer of an Alfisol in Lexington, KY. The roots of four species were packed into nylon-wrapped tubes with soil and …

Avoiding Misleading Estimates Of Among-Individual Variance Caused By Non-Random Sampling Of Individuals In A Changeable Environment, Denis Réale, Hassen Allegue, Yimen G. Araya-Ajoy, Ned A. Dochtermann, Shinichi Nakagawa, Joel L. Pick, Holger Schielzeth, David F. Westneat, Niels J. Dingemanse

Biology Faculty Publications

Animal ecologists frequently quantify variance in hierarchically structured traits in wild populations. Importantly, phenotypic plasticity within the period of measurement can modify the trait of interest in response to various unmeasured, temporally or spatially changeable, environmental conditions. Non-random sampling among units of the random effect (e.g. individuals) regarding the environment at issue may lead to estimates of the variance among ((Formula presented.)) or within ((Formula presented.)) such units that conflate several types of processes. This mixing of underlying biology can affect interpretations of the random effect variance. Here, we explore the conditions leading to this situation and assess potential solutions …

Role Of Succinate Dehydrogenase In Age-Related Th17 Inflammation, Evelyn Ocegueda, Gabrielle Chase, Michaella Niceforo, Aida Javidan, Lydia Gugliuzza, Jingting Yu, Rachel Y. Kang, Ava Lankowski, Olivia Stefanik, Kailey Leclerc, Yolander Valentine, Micah J. Drummond, Jude T. Deeney, Josephine S. Modica-Napolitano, Elizabeth A. Proctor, Hatice Hasturk, Barbara S. Nikolajczyk, Leena P. Bharath

Markey Cancer Center Faculty Publications

Age-related cellular changes negatively impact CD4⁺ T cell function. Our prior work showed that mitochondrial complex II (succinate dehydrogenase [SDH]) expression was upregulated in T cells from older (O) adults (60–80 years old). T cells from older adults also produced higher amounts of cytokines generally considered proinflammatory, such as Th17 cytokines IL-17A/F and IL-21, and the Th-17-supportive cytokine IL-6, compared to T cells from younger (Y) adults (25–40 years old). The objective of our study is to evaluate whether hyperactivation of SDH is required for the induction of proinflammatory cytokines and the mechanistic link between SDH and Th17 cytokine …

Structural Assembly Of The Pas Domain Drives The Catalytic Activation Of Metazoan Pask, Sajina Dhungel, Michael Xiao, Rajesh Rajaian Pushpabai, Chintan K. Kikani

Biology Faculty Publications

PAS domains are ubiquitous sensory modules that transduce environmental signals into cellular responses through tandem PAS folds and PAS-associated C-terminal (PAC) motifs. While this conserved architecture underpins their regulatory roles, here we uncover a structural divergence in the metazoan PAS domain-regulated kinase (PASK). By integrating evolutionary-scale domain mapping with deep learning-based structural models, we identified two PAS domains in PASK, namely PAS-B and PAS-C, in addition to the previously known PAS-A domain. Unlike canonical PAS domains, the PAS fold and PAC motif in the PAS-C domain are spatially segregated by an unstructured linker, yet a functional PAS module is assembled …

Advancing Root Trait Measurements For A Deeper Understanding Of Soil Biogeochemistry, Brian Rinehart

Theses and Dissertations--Plant and Soil Sciences

Climate change is disrupting (agro)ecosystems, with the potential for significant losses in crop productivity and ecosystem resilience due to changes in water and biogeochemical cycling. These disruptions can also release more carbon dioxide into the atmosphere through the degradation of soil organic matter. Plant roots have emerged as a potential tool for mitigating these effects, given their roles in plant function and in driving soil biogeochemistry. While this interest is growing, our ability to implement root trait selection is limited due to constraints on characterizing roots and limited clarity on the effects of traits on plant and ecosystem function. My …

Pepper, Robert Yokel

Aluminum in Beverages and Foods: A Compilation

No abstract provided.

Other Spices, Alphabetically, Robert Yokel

Aluminum in Beverages and Foods: A Compilation

No abstract provided.

Perspectives On The Clinical Use Of Anti-Amyloid Therapy For The Treatment Of Alzheimer's Disease: Insights From The Fields Of Cancer, Rheumatology, And Neurology, Gregory Jicha, Erin Abner, Elif Pinar Coskun, Mark Huffmyer, Thomas Tucker, Peter T. Nelson

Neurology Faculty Publications

Introduction: The advent of disease-modifying therapies for Alzheimer’s disease (AD) has raised many questions and debates in the field as to the clinical benefits, risks, and costs of such therapies. The controversies have resulted in the perception that many clinicians are apprehensive about prescribing these medications to their patient populations. There also remains widespread uncertainty as to the economic impact, cost benefit ratio, and safety oversight for use of these medications in standard clinical care settings.

Methods: To contextualize such issues, the present study compared anti-amyloid biologic therapy (lecanemab) to four commonly used biologic agents in other fields, including trastuzumab …

Analysis Of The Thermal Aging Kinetics Of Tallow, Chicken Oil, Lard, And Sheep Oil, Yun-Chuan Hsieh, Hao Ouyang, Yulin Zhang, Donyau Chiang, Fuqian Yang, Hsin-Lung Chen, Sanboh Lee

Chemical and Materials Engineering Faculty Publications

Understanding the thermal aging kinetics of animal oils is of vital importance in the storage and applications of animal oils. In this work, we use four different techniques, including UV-Vis spectrometry, viscometry, impedance spectroscopy, and acid–base titration, to study the thermal aging kinetics of tallow, chicken oil, lard, and sheep oil in the temperature range from 120 ◦C to 180 ◦C. The evolutions of the UV-Vis absorbance, dynamic viscosity, electric impedance, and acid titration are discussed with the defect kinetics. The evolutions of the color centers, defects for dynamic viscosity, and electric dipoles follow second-order, first-order, and zero-order kinetics, respectively. …

Advanced Mathematical Graph-Based Machine Learning And Deep Learning Models For Drug Design, Farjana Tasnim Mukta

Theses and Dissertations--Mathematics

Drug discovery is a highly complicated and time-consuming process. One of the main challenges in drug development is predicting whether a drug-like molecule will interact with a specific target protein. This prediction accelerates target validation and drug development. Recent research in biomolecular sciences has shown significant interest in algebraic graph-based models for representing molecular complexes and predicting drug-target binding affinity. In this thesis, we present algebraic graph-based molecular representations to create data-driven scoring functions (SF) using extended atom types to capture wide-range interactions between targets and drug candidates. Our model employs multiscale weighted colored subgraphs for the protein-ligand complex, colored …

Biodiversity And The Ecological Role Of Grasslands Environment And Territory, Elimane Lo, Makhmutov Serguei, Badim Almazov

IGC Proceedings (1977-2023)

Biodiversity is all the living species that inhabit the planet: plants, animals, microorganisms, but also their genetic diversity and all the ecosystems in which they live. The preservation of biodiversity is vital, because the organisms that constitute it participate in the major ecological cycles of air, soil and water It is today recognized for its intrinsic value and for the functions it provides in ecosystems. The grassland and the hedges that surround them play a key role in carbon storage, water purification, biodiversity and the typicity of landscapes. They partly provide the plants necessary for feeding herds of herbivores (cattle, …

Stat3 Modulates Cd4+ T Mitochondrial Dynamics And Function In Aging, Emelia Zukowski, Marco Sannella, Jack Donato Rockhold, Gabriella Kalantar, Jingting Yu, Sara Santacruz-Calvo, Madison K. Kuhn, Nasun Hah, Ling Ouyang, Tzu-Wen Wang, Lyanne Murphy, Heather Marszalkowski, Kaleigh Gibney, Micah J. Drummond, Elizabeth A. Proctor, Hatice Hasturk, Barbara S. Nikolajczyk, Leena P. Bharath

Markey Cancer Center Faculty Publications

Aging promotes numerous intracellular changes in T cells that impact their effector function. Our data show that aging promotes an increase in the localization of STAT3 to the mitochondria (mitoSTAT3), which promotes changes in mitochondrial dynamics and function and T-cell cytokine production. Mechanistically, mitoSTAT3 increased the activity of aging T-cell mitochondria by increasing complex II. Limiting mitoSTAT3 using a mitochondria-targeted STAT3 inhibitor, Mtcur-1 lowered complex II activity, prevented age-induced changes in mitochondrial dynamics and function, and reduced Th17 inflammation. Exogenous expression of a constitutively phosphorylated form of STAT3 in T cells from young adults mimicked changes in mitochondrial dynamics and …

Microcalorimeter As A Biologic Activity Monitor For The Study Of Brachiaria Brizantha Seed Germination Process, M. A. Barboza, P. L. O. Volpe, R. Usberti, J. F. G. Faigle, R. H. Aguiar

IGC Proceedings (1977-2023)

Calorimetry helps better understanding of biological processes (Calvet & Prat, 1963). Very sensitive thermal sensors and microcalorimeters allow real time investigation and monitoring heat production of seed germination but few experiments have been performed in this area (Sigstad & Prado, 1999). Moreover, experimental procedures correlating germination phenomena and chemical thermodynamics are exceptional (Barboza, 2002). One can detect calorimetrically the heat flow produced during seed germination and compare the results with data recorded using standard germination methodology (ISTA, 1985). Seed germination and the biomass increase respiration and determination of the energy involved aids understanding of the energetic cycle involved. This work …

Development And Biological Evaluation Of Selective Small-Molecule Inhibitors Of The Human Cytochrome P450 1b1, Austin Hachey

Theses and Dissertations--Chemistry

The human cytochrome P450 1B1 (CYP1B1) is an emerging target for small- molecule therapeutics. Several solid tumors overexpress CYP1B1 to the degree that it has been referred to as a universal tumor antigen. Conversely, its expression is low in healthy tissues. CYP1B1 may drive tumorigenesis through promoting the formation of reactive toxins from environmental pollutants or from endogenous hormone substrates. Additionally, the expression of CYP1B1 in tumors is associated with resistance to several common chemotherapies and with poor prognoses in cancer patients. However, inhibiting CYP1B1 with small molecules has been demonstrated in cellular and murine model systems to reverse this …

Investigating Ru(Ii) Anticancer Agents With Non-Canonical Mechanisms Of Action, Richard Joseph Mitchell

Theses and Dissertations--Chemistry

Platinum(Pt)-based agents are used in approximately 50% of all cancers that are treated with chemotherapy. Unfortunately, the dose-limiting toxicity of these agents remains problematic for patients undergoing treatment. Additionally, Pt(II) therapeutics suffer from transporter-dependent uptake, limited chemical functionalization, and high susceptibility to inactivation by free thiols within the cytosol. Developing small molecules with non-canonical mechanisms of action is one strategy that can be employed to circumvent these limitations. Utilization of coordination complexes with ruthenium(Ru) metal centers is one attractive strategy. Ru(II) compounds are often octahedral, facilitating greater accessibility for chemical diversity; Ru(II) complexes use passive transport and transferrin-mediated transport for …

Multivariate Statistical Modeling For Radio-Genomics Study, Tiantian Zeng

Theses and Dissertations--Statistics

Radiogenomics is a new direction in cancer research that focuses on the associations among radiomics, genomics and clinical outcome. Currently, the major challenge for Radiogenomics lies in the effective integration of genomics and imaging data for promising clinical outcome prediction. Herein, we propose a multivariate joint model that can integrate imaging and genomic data for better predicting the clinical outcome. Specifically, we jointly consider two multivariate group lasso models, one regresses imaging features on genomic features, and the other regresses patient’s clinical outcome on genomic features. An L1 penalty term is introduced for each variable, and weight in the penalty …

Development Of Zafirlukast Derivatives Active Against Porphyromonas Gingivalis, Kaitlind C. Howard

Theses and Dissertations--Pharmacy

Periodontal diseases are inflammatory diseases that can lead to damage of the soft tissue and bone supporting the teeth. Gingivitis is the reversible early stage of gum disease, which consist of gums that bleed when brushing or flossing teeth. When left untreated, the preventable but irreversible late stage of periodontal disease, periodontitis, can lead teeth to loosen or even fall out. Oral bacterial species, such as the Gram-negative anaerobic pathogen Porphyromonas gingivalis trigger these inflammatory diseases through oral dysbiosis. P. gingivalis is a keystone pathogen, meaning that the damage caused by P. gingivalis is not proportional to its abundance. Treatment …

Mass Spectrometric Analysis Of Lignin Model Compounds: Fundamental Investigations Of Ionization And Supramolecular Interactions For Lignocellulosic Biomass Applications, Kimberly Dean

Theses and Dissertations--Chemistry

Lignocellulosic biomass is pivotal for the development of renewable energy sources and materials essential to mitigate the exploitation of fossil fuels causing climate change and environmental pollution issues. The conversion of biomass into fuel requires the hydrolysis of cellulose and a biproduct of this process is the isolation of millions of tons of lignin as biorefinery waste. Lignin is a complex high molecular weight polymer whose structure remains undefined and critically limits potential industrial applications of lignocellulosic biomass. The advancement of analytical methods for structural elucidation of lignin and its ensemble of phenolic compounds is therefore essential to advance this …

Structural Basis Of Bacterial Flagellin For Naip5 Binding And Nlrc4 Inflammasome Activation And The Mechanism Of Flagellin Induced Release Of Cytokines In Vivo, Jian Cui

Theses and Dissertations--Chemistry

The bacterial flagellum is a whip-like structure that protrudes from the cell membrane and is one of the most complex and dynamic biological molecular machines that propels bacteria to swim toward beneficial environments and the sites of infection. It is composed of a basal body, a hook, and a long filament. The flagellar filament contains thousands of copies of the protein flagellin (FliC) monomer arranged helically and ending with a filament cap composed of oligomer protein FliD. The overall structure of the filament core is preserved across bacterial species, while the outer domains exhibit high variability, and in some cases …

Biological Control And Integrated Rangeland Weed Management, Linda M. Wilson

IGC Proceedings (1977-2023)

No abstract provided.

Co-Teaching Practices Of General And Special Educators In Secondary Science Classrooms, Samantha Jolene Ringl

Theses and Dissertations--Education Sciences

The purpose of this study was to establish current levels of co-teaching implementation and to explore teachers’ perceptions of co-teaching and co-teacher relationships. This study also examined whether there is a relationship between teachers’ perception of their co-teaching relationship and their perception of co-teaching implementation in their classrooms. Analysis revealed that teachers generally have positive perceptions of their co-teaching partners and relationships, and these perceptions did not significantly differ between science teachers and special educators. However, this study found that teachers are not regularly planning lessons together, sharing the workload in the classroom, or choosing co-teaching models together. This study …

Rational Design Of Small Molecule Disruptors Of Protein-Protein Interactions: Pd-1/Pd-L1; C-Myc, Arid4b, Samuel Ofori

Theses and Dissertations--Chemistry

Protein-protein interactions (PPIs) are vital to many biological processes, including gene expression, and immune reactions to pathogens. There are approximately 650,000 PPIs in humans with pertinent physiological functions. Aberrant expression of PPIs lead to improper function and contribute to a plethora of disease conditions including cancer. Thus, PPIs represent an enormous target space for drug discovery and chemical probes. Direct targeting of clinically relevant PPIs with small molecules remains an unmet medical need. Development of small-molecule inhibitors of PPIs is a challenging enterprise and in most cases considered undruggable due to large protein surfaces, lack of deep binding pockets and …

Design, Synthesis, And Anticancer Properties Of Ru(Ii) Complexes With Organometallic, “Expanded” Bipyridine, And O,O’-Chelating Ligands, Raphael Ryan

Theses and Dissertations--Chemistry

Cancer is a worldwide public health crisis that requires new and improved drugs to be developed to extend survival rates and improve quality of life for the patient. Platinum-based drugs are used in approximately 50% of cancer treatment regimens. These drugs are highly effective in many kinds of cancer; however, cancers can develop platinum resistance and these drugs have troubling side effects that reduced their use and efficacy. To overcome these disadvantages, many other metals have been studied for their anticancer properties. Notably, the anticancer properties of ruthenium-based agents have drawn considerable attention with multiple ruthenium complexes entering clinical trials. …

Icdp Workshop On The Lake Tanganyika Scientific Drilling Project: A Late Miocene–Present Record Of Climate, Rifting, And Ecosystem Evolution From The World's Oldest Tropical Lake, James M. Russell, Philip Barker, Andrew Cohen, Sarah Ivory, Ishmael Kimirei, Christine Lane, Melanie Leng, Neema Maganza, Michael M. Mcglue, Emma Msaky, Anders Noren, Lisa Park Boush, Walter Salzburger, Christopher Scholz, Ralph Tiedemann, Shaidu Nuru, Lake Tanganyika Scientific Drilling Project Consortium

Earth and Environmental Sciences Faculty Publications

The Neogene and Quaternary are characterized by enormous changes in global climate and environments, including global cooling and the establishment of northern high-latitude glaciers. These changes reshaped global ecosystems, including the emergence of tropical dry forests and savannahs that are found in Africa today, which in turn may have influenced the evolution of humans and their ancestors. However, despite decades of research we lack long, continuous, well-resolved records of tropical climate, ecosystem changes, and surface processes necessary to understand their interactions and influences on evolutionary processes. Lake Tanganyika, Africa, contains the most continuous, long continental climate record from the mid-Miocene …

Establishment Of High-Efficiency Agrobacterium-Mediated Transformation Of Callus Derived From Sehima Nervosum, An Important Range Grass Species, Krishna Kumar Dwivedi, Tanu Sonkar, R. Katiyar, Auji Radhakrishna, P. Kaushal

IGC Proceedings (1977-2023)

Sehima nervosum is one of the important rangeland grass in India, It is commonly known as Saen grass in India, white grass in Australia, and has also been reported from the Central East Africa and Sudan. It is a good forage grass and maybe utilized for grazing as well as for hay preparation. It is a perennial grass, prefers hot and dry climate and survive even in limited rainfalls. As this natural grass is found inherently rich in precursors for several industrially important biomolecules, fractionation of these precursors seems to be a promising endeavour. Production of nutraceuticals (prebiotic xylo-oligosaccharides) from …

The Values Of Warm-Season Native Perennial Grasses Grown For Pasture Or Biofuel In The Southern Great Plains, Usa, James K. Rogers, Bryan Nichols, Jon T. Biermacher, Jagadeesh Mosali

IGC Proceedings (1977-2023)

The Renewable Fuel Standard under the Energy Independence and Security Act of 2007 mandated the production of 136 billion liters of cellulosic biofuel by 2022. Switchgrass (Panicum virgatum) has been identified as a primary feedstock because it is a perennial, produces high yields and is adapted to a wide environmental range. Development of the cellulosic biofuel industry has been slow. A reason for this slow development is lack of available feedstock driven by lack of a developed market. Rather than considering switchgrass only as a dedicated biofuel feedstock, we examined its potential both for grazing and biofuel feedstock. …

Single-Degree-Of-Freedom Experiments Demonstrating Electromagnetic Formation Flying For Small Satellite Swarms Using Piecewise-Sinusoidal Controls, Ajin Sunny

Theses and Dissertations--Mechanical and Aerospace Engineering

This thesis presents a decentralized electromagnetic formation flying (EMFF) control method using frequency-multiplexed sinusoidal control signals. We demonstrate the EMFF control approach in open-loop and closed-loop control experiments using a single-degree-of-freedom testbed with an electromagnetic actuation system (EAS). The EAS sense the relative position and velocity between satellites and implement a frequency-multiplexed sinusoidal control signal. We use a laser-rangefinder device to capture the relative position and an ARM-based microcontroller to implement the closed-loop control algorithm. We custom-design and build the EAS that implements the formation control in one dimension. The experimental results in this thesis demonstrate the feasibility of the …

Oleic Acid Vesicles: Formation, Mechanisms Of Reactivity, And Uses In Determination Of Terpene Activity, Laura A. Walther

Theses and Dissertations--Chemistry

This dissertation will focus on the volatile compounds released upon the burning of incense which are numerous and varied. The first part of this dissertation is the gas chromatography-mass spectral (GC-MS) analysis of burning incense collected via solid phase microextraction (SPME) with the aim of developing a library of compounds found in incense as used in the Orthodox church.

The second part of this dissertation has the aim of developing a method for forming oleic acid bilayer vesicle membranes and a fluorescence spectroscopy method by which the reactivities of these vesicles can be analyzed. These reactivities include permeability, fluidity, aggregation, …

Intranasal Rapamycin Ameliorates Alzheimer-Like Cognitive Decline In A Mouse Model Of Down Syndrome, Antonella Tramutola, Chiara Lanzillotta, Eugenio Barone, Andrea Arena, Ilaria Zuliani, Luciana Mosca, Carla Blarzino, D. Allan Butterfield, Marzia Perluigi, Fabio Di Domenico

Chemistry Faculty Publications

Background: Down syndrome (DS) individuals, by the age of 40s, are at increased risk to develop Alzheimer-like dementia, with deposition in brain of senile plaques and neurofibrillary tangles. Our laboratory recently demonstrated the disturbance of PI3K/AKT/mTOR axis in DS brain, prior and after the development of Alzheimer Disease (AD). The aberrant modulation of the mTOR signalling in DS and AD age-related cognitive decline affects crucial neuronal pathways, including insulin signaling and autophagy, involved in pathology onset and progression. Within this context, the therapeutic use of mTOR-inhibitors may prevent/attenuate the neurodegenerative phenomena. By our work we aimed to rescue mTOR signalling …

Bio-Inspired Hybrid Vibration Control Methodology For Intelligent Isolated Bridge Structures, Mariantonieta Gutierrez Soto

Civil Engineering Faculty Publications

Inspired by evolutionary game theory, the biological game of replicator dynamics is investigated for vibration control of bridge structures subjected to earthquake excitations. Replicator dynamics can be interpreted economically as a model of imitation of successful individuals. This paper uses replicator dynamics to reduce vibrations while optimally allocating the control device forces. The control algorithm proposed is integrated with a patented neural dynamic optimization algorithm to find optimal growth rate values with the goal of achieving satisfactory structural performance with minimum energy consumption. A model is described for hybrid vibration control of smart highway bridge structures subjected to earthquake loading.