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Role Of Angiotensin Converting Enzyme 2 (Ace2) In Obesity-Associated Hypertension, Manisha Gupte

University of Kentucky Doctoral Dissertations

The purpose of this research was to determine whether adipocytes express ACE2 and its role in obesity-associated hypertension with diet-induced obesity.

To determine if ACE2 was expressed in adipose tissue and its regulation in the setting of diet-induced obesity, we fed male mice either a low fat (LF) or high fat (HF) diet acutely (1 week) or chronically ( 4 months). We demonstrated that ACE2 was regulated specifically in adipose tissue with consumption of a HF diet. However, with chronic HF feeding adipose ACE2 was dysregulated resulting in activation of the systemic RAS and increased blood pressure.

To determine the …

Cartesian Skepticism As Moral Dilemma, Jennifer Woodward

University of Kentucky Doctoral Dissertations

I argue that despite the fact that there can be no strong refutation of skepticism it remains that ignoring skeptical hypotheses and relying on one’s sensory experience are both sound epistemic practices. This argument comes in the form of arguing that we are justified in ignoring skeptical hypotheses on the grounds that (1) they are merely logically possible, and (2) the merely logically possible is rarely relevant in the context of everyday life. I suggest that (2) is true on the grounds that the context of everyday life is one in which our epistemic pursuit of truth is mixed with …

Education Is Like A Magic Carpet: Transfer Perceptions Of Urban Latino Community College Students, Catharine Mary Anne Penfold Navarro

University of Kentucky Doctoral Dissertations

Latino students are the only ethnic group more likely to enroll in community colleges than four-year institutions. However, they transfer to four-year institutions at much lower rates than their white counterparts. This gap in transfer rates for Hispanic students is of significant concern to higher education researchers and policy makers because of its broad impact on overall educational attainment for Hispanics.

Research on Latino transfer rates has focused predominantly on quantitative data, which paints a clear picture of what is happening, but falls short in explaining why transfer rates continue to be low. Researchers have offered both structural (social reproduction …

Genotoxin-Induced Acetylation Of The Werner Syndrome Protein (Wrn) And Effect On Its Dna Metabolic Function, Enerlyn Meliza Lozada Santiago

University of Kentucky Doctoral Dissertations

Loss of function of the WRN protein causes the genetic disorder Werner Syndrome that is characterized by increased cancer and premature aging. WRN belongs to the RecQ helicase family that plays key roles in preventing genome instability. In response to treatment with genotoxins, WRN is subject to post-translational modification. The relationship of post-translational modification of WRN with its function in DNA metabolism is unknown. There is accumulating evidence suggesting that WRN contributes to the maintenance of genomic integrity through its involvement in DNA replication. Consistent with this notion, WS cells are sensitive to DNA replication inhibitors and DNA damaging agents …

Identification Of Activities Involved In Cag/Ctg Repeat Instability, Nelson Lap Shun Chan

University of Kentucky Doctoral Dissertations

CAG/CTG repeat instability is associated with at least 14 neurological disorders, including Huntington’s disease and Myotonic dystrophy type 1. In vitro and in vivo studies have showed that CAG/CTG repeats form a stable hairpin that is believed to be the intermediate for repeat expansion and contraction.

Addition of extra DNA is essential for repeat expansion, so DNA synthesis is one of the keys for repeat expansion. In vivo studies reveal that 3’ CTG slippage with subsequent hairpin formation (henceforth called the 3’ CTG slippage hairpin) occurs during DNA synthesis. It is proposed that hairpin tolerance machinery is activated because prolonged …

Identifying Component-Processes Of Executive Functioning That Serve As Risk Factors For Alcohol-Related Aggression, Aaron John Godlaski

University of Kentucky Doctoral Dissertations

The present investigation determined how different component-processes of executive functioning (EF) acted as risk factors for intoxicated aggression. Participants were 512 (246 men and 266 women) healthy social drinkers between 21 and 35 years of age. EF was measured using the Behavior Rating Inventory of Executive Functioning – Adult Version (BRIEF-A; Roth, Isquith, & Gioia, 2005) that assesses nine EF components. Following the consumption of either an alcohol or a placebo beverage, participants were tested on a modified version of the Taylor Aggression Paradigm (Taylor, 1967) in which mild electric shocks were received from, and administered to, a …

Using Linked Household-Level Datasets To Explain Consumer Response To Bse In Canada, Xin Wang

University of Kentucky Doctoral Dissertations

Household-level Canadian meat purchases from 2002-2008, a Food Opinions Survey conducted in 2008 at the national level and household-level egg purchases from 2002-2005 in Alberta and Ontario were used to explore consumer responses to Bovine Spongiform Encephalopathy (BSE) in Canada.

The opinions survey focused on nutritional priorities, general and specific food safety concerns, and trust in government and food industry decision makers. The egg data set contained specific product information allowing us to distinguish purchases of conventional eggs from those of value-added eggs with perceived health attributes. Thus, the egg purchase data appeared to be an interesting proxy of revealed …

Synthesis And Energy Applications Of Oriented Metal Oxide Nanoporous Films, Qingliu Wu

University of Kentucky Doctoral Dissertations

This dissertation mainly addresses the synthesis of well-ordered mesoporous titania thin films by dip coating with PEO-PPO-PEO triblock copolymer surfactant template P123. Because P123 is composed of poly(ethylene oxide) [PEO] and poly(propylene oxide) [PPO] blocks, concentrations of ingredients are adjusted to tune the films’ wall thickness, pore size and mesophase. Structural changes are consistent with partitioning of species among PEO blocks, PPO blocks, and the PEO/PPO interface. Titanates localize near PEO and increase wall thickness (by 5 nm to 7 nm). Depending on aging temperature, PPG either swells the PPO cores (when it is hydrophobic) or introduces large (>200 …

Post-Transcriptional Regulation Of Afp And Igm Genes, Lilia M. Turcios

University of Kentucky Doctoral Dissertations

Gene expression can be regulated at multiple steps once transcription is initiated. I have studied two different gene models, the α-Fetoprotein (AFP) and the immunoglobulin heavy chain (IgM) genes, to better understand post-transcriptional gene regulation mechanisms. The AFP gene is highly expressed during fetal liver development and dramatically repressed after birth. There is a mouse strain-specific difference between adult levels of AFP, with BALB/cJ mice expressing 10 to 20-fold higher levels compared to other mouse strains. BALB/cJ mice express low levels of Zhx2 and thus incompletely repress AFP. Despite differences in steady state AFP mRNA levels in the adult liver …

Enhancements To The Modified Composite Pattern Method Of Structured Light 3d Capture, Charles Joseph Casey

University of Kentucky Doctoral Dissertations

The use of structured light illumination techniques for three-dimensional data acquisition is, in many cases, limited to stationary subjects due to the multiple pattern projections needed for depth analysis. Traditional Composite Pattern (CP) multiplexing utilizes sinusoidal modulation of individual projection patterns to allow numerous patterns to be combined into a single image. However, due to demodulation artifacts, it is often difficult to accurately recover the subject surface contour information. On the other hand, if one were to project an image consisting of many thin, identical stripes onto the surface, one could, by isolating each stripe center, recreate a very accurate …

Nation, Fantasy, And Mimicry: Elements Of Political Resistance In Postcolonial Indian Cinema, Aparajita Sengupta

University of Kentucky Doctoral Dissertations

In spite of the substantial amount of critical work that has been produced on Indian cinema in the last decade, misconceptions about Indian cinema still abound. Indian cinema is a subject about which conceptions are still muddy, even within prominent academic circles. The majority of the recent critical work on the subject endeavors to correct misconceptions, analyze cinematic norms and lay down the theoretical foundations for Indian cinema. This dissertation conducts a study of the cinema from India with a view to examine the extent to which such cinema represents an anti-colonial vision. The political resistance of Indian films to …

Modeling, Analysis, And Simulation Of Discrete-Continuum Models Of Step-Flow Epitaxy: Bunching Instabilities And Continuum Limits, Nicholas O. Kirby

University of Kentucky Doctoral Dissertations

Vicinal surfaces consist of terraces separated by atomic steps. In the step-flow regime, deposited atoms (adatoms) diffuse on terraces, eventually reaching steps where they attach to the crystal, thereby causing the steps to move. There are two main objectives of this work. First, we analyze rigorously the differences in qualitative behavior between vicinal surfaces consisting of infinitely many steps and nanowires whose top surface consists of a small number of steps bounded by a reflecting wall. Second, we derive the continuum model that describes the macroscopic behavior of vicinal surfaces from detailed microscopic models of step dynamics.

We use the …

Computational Analyses Of The Uptake And Distribution Of Carbon Monoxide (Co) In Human Subjects, Kinnera Chada

University of Kentucky Doctoral Dissertations

Carbon monoxide (CO) is an odorless, colorless, tasteless gas that binds to hemoglobin with high affinity. This property underlies the use of low doses of CO to determine hemoglobin mass (M_Hb) in the fields of clinical and sports medicine. However, hemoglobin bound to CO is unable to transport oxygen and exposure to high CO concentrations is a significant environmental and occupational health concern. These contrasting aspects of CO—clinically useful in low doses but potentially lethal in higher doses—mandates a need for a quantitative understanding of the temporal profiles of the uptake and distribution of CO …

Cooperative And Antagonistic Roles For Heterochromatin Proteins In Transcriptional Regulation Of The Drosophila Sex Determination Masterswitch Gene, Hui Li

University of Kentucky Doctoral Dissertations

HOAP was originally identified as a component of an ORC-containing multi-protein complex of Heterochromatin Protein 1 (HP1) from early Drosophila embryos. HOAP immunostaining showed prominent association of it with telomeres, and mutants for HOAP (cav¹) showed it functions along with HP1 in forming a telomere capping complex that prevents telomeric fusions.

Weaker HOAP immunostaining is also observed in regions of pericentric heterochromatin and euchromatin. To examine the role of HOAP at these non-telomeric sites, we applied Affymetric Drosophila Genome Arrays to undertake a microarray expression profiling study of genes that are mis-expressed in cav¹ mutant larvae. The …

Effects Of Adipogenic Compounds On Growth Performance, Hormonal Status And Fat Deposition Of Finishing Beef Steers, Susanna Elizabeth Kitts

University of Kentucky Doctoral Dissertations

Processes that regulate site of fat deposition in beef cattle are poorly understood. For the producer to procure the greatest profit, it is ideal to maximize intramuscular fat. Furthermore, to understand the physiological mechanisms affecting fat depots, it is necessary to evaluate hormones involved in growth regulation. Using a 2 x 2 factorial design of treatments, four experiments were conducted to examine two adipogenic compounds, chlortetracycline and dexamethasone. Synovex-S® and Revalor-S® were used to investigate potential interactions between growth implants and adipogenic compound. Growth performance, carcass quality, organ and fat mass and plasma hormone concentrations were measured in these studies. …

Strategic Flexibility: Household Ecologies Of Ful’Be In Tanout, Niger, Karen Marie Greenough

University of Kentucky Doctoral Dissertations

(Agro)pastoralism in Sahelian Niger, as elsewhere, operates through household enterprises. Katsinen-ko’en (Fulбe) households, interconnected within kin and community networks, utilize a range of flexible strategies to manage a variety of ecological and economic risks. This dissertation argues that (agro)pastoralist households and communities maintain or improve viability in risky environments first by employing various mobility patterns, among other strategies, and relying on the tightly knit interdependence between household and herd. Secondly, households that most successfully sustain a cooperative integrity (i.e. partnerships between husband and wife, or wives, and parents and children) to negotiate decisions and strategies best withstand adversities such as …

Production, Exchange And Social Interaction In The Green River Region Of Western Kentucky: A Multiscalar Approach To The Analysis Of Two Shell Midden Sites, Christopher R. Moore

University of Kentucky Doctoral Dissertations

The Green River region of western Kentucky has been a focus of Archaic period research since 1915. Currently, the region is playing an important role in discussions of Archaic hunter-gatherer cultural complexity. Unfortunately, many of the larger Green River sites contain several archaeological components ranging from the Early to Late Archaic periods. Understanding culture change requires that these multiple components somehow be sorted and addressed individually.

Detailed re-analyses of Works Progress Administration (WPA) era artifact collections from two archaeological sites in the Green River region – the Baker (15Mu12) and Chiggerville (15Oh1) shell middens – indicate that these sites are …

Some Contributions To The Censored Empirical Likelihood With Hazard-Type Constraints, Yanling Hu

University of Kentucky Doctoral Dissertations

Empirical likelihood (EL) is a recently developed nonparametric method of statistical inference. Owen’s 2001 book contains many important results for EL with uncensored data. However, fewer results are available for EL with right-censored data. In this dissertation, we first investigate a right-censored-data extension of Qin and Lawless (1994). They studied EL with uncensored data when the number of estimating equations is larger than the number of parameters (over-determined case). We obtain results similar to theirs for the maximum EL estimator and the EL ratio test, for the over-determined case, with right-censored data. We employ hazard-type constraints which are better able …

Cross-Border Shopping: Implications For State Fiscal Competition In Multiple Tax Instruments, Kusum Singh

University of Kentucky Doctoral Dissertations

This dissertation investigates whether consumers’ cross-border shopping due to interstate commodity tax differentials influence counties’ economic activity and states’ strategic competition in multiple tax policies.

First, I examine how own and the nearest neighboring states’ commodity tax rates affect counties’ retail activity. Particularly, in contrast to many previous studies, I examine whether the distance to the state border influences the responsiveness of counties’ retail activity to sales and excise taxes of own and the nearest neighboring states. Since the costs of avoiding state commodity taxes are presumably lower along borders, the impacts of state commodity taxes on retail activity may …

Comparison Of Plant‐Adapted Rhabdovirus Protein Localization And Interactions, Kathleen Marie Martin

University of Kentucky Doctoral Dissertations

Sonchus yellow net virus (SYNV), Potato yellow dwarf virus (PYDV) and Lettuce Necrotic yellows virus (LNYV) are members of the Rhabdoviridae family that infect plants. SYNV and PYDV are Nucleorhabdoviruses that replicate in the nuclei of infected cells and LNYV is a Cytorhabdovirus that replicates in the cytoplasm. LNYV and SYNV share a similar genome organization with a gene order of Nucleoprotein (N), Phosphoprotein (P), putative movement protein (Mv), Matrix protein (M), Glycoprotein (G) and Polymerase protein (L). PYDV contains an additional predicted gene between N and P, denoted as X, that has an unknown function. In order to gain …

Development Of Luminescent Sensing Systems With Clinical Applications, Daniel F. Scott

University of Kentucky Doctoral Dissertations

As the move towards the miniaturization of many diagnostic and detection systems continues, the need for increasingly versatile yet sensitive labels for use in these systems also grows. Luminescent reporters provide us with a solution to many of the issues at hand through their unique and favorable characteristics. Bioluminescent proteins offer detection at extremely low concentrations and no interference from physiological fluids leading to excellent detection limits, while the vast number of fluorescent proteins and molecules available allows the opportunity to select a tailored reporter for a specific task. Both provide relatively simply instrumentation requirements and have exhibited great promise …

Firm Bidding Behavior In Highway Procurement Auctions: An Analysis Of Single-Bid Contracts, Tacit Collusion, And The Financial Impact On Kentucky, David R. Barrus

University of Kentucky Doctoral Dissertations

Recently, the American Association of State Highway and Transportation Officials (AASHTO) indicated lack of competition and single-bid contracts in asphalt paving as a major issue facing state transportation departments. Single-bid contracts indicate a lack of competition which increases costs to state and local governments. During the period from 2005-2007 in Kentucky, 42 percent of all bids were awarded with only one firm bidding on the project. Of the asphalt paving jobs, 63 percent of those jobs were awarded to a single bidder.

The analysis of this dissertation focuses on detecting tacit collusion in asphalt paving jobs in Kentucky. A focal …

Novel Mechanisms In Inflammatory Bowel Disease, Razvan I. Arsenescu

University of Kentucky Doctoral Dissertations

Inflammatory Bowel Diseases, Crohn's Disease and Ulcerative colitis, are idiopathic chronic conditions with multifactorial determinants. In general, terms, intestinal inflammation results from abnormal host-microbe interactions. Alterations in homeostasis involve host genetic factors, environmental cues and unique luminal microbial niches. We have examined the coordinated expressions of several molecular targets relevant to the mucosal immune system and identified signature biomarkers of IBD. Qualitative and quantitative changes in the composition of microbiota can be related to unique immuno-phenotypes. This in turn can have more systemic effects that involve energy metabolism. Adiponectin, an adipose tissue derived adipokine, can restore cellular ATP levels and …

Solution Phase And Membrane Immobilized Iron-Based Free Radical Reactions: Fundamentals And Applications For Water Treatment, Scott Romak Lewis

University of Kentucky Doctoral Dissertations

Membrane-based separation processes have been used extensively for drinking water purification, wastewater treatment, and numerous other applications. Reactive membranes synthesized through functionalization of the membrane pores offer enhanced reactivity due to increased surface area at the polymer-solution interface and low diffusion limitations. Oxidative techniques utilizing free radicals have proven effective for both the destruction of toxic organics and non-environmental applications. Most previous work focuses on reactions in the homogeneous phase; however, the immobilization of reactants in membrane pores offers several advantages. The use of polyanions immobilized in a membrane or chelates in solution prevents ferric hydroxide precipitation at near-neutral pH, …

Going On Otor: Disaster, Mobility, And The Political Ecology Of Vulnerability In Uguumur, Mongolia, Daniel J. Murphy

University of Kentucky Doctoral Dissertations

Post-socialist states have increasingly adopted rural governance and resource management policies framed around the concepts of decentralization, devolution, and de-concentration in which formerly central state powers are transferred to lower, more local levels of governance. In more recent incarnations, these policies have become inspired by neo-liberal discourses of minimal government, self-rule, and personal responsibility. Increasingly, the social science literature has argued that such forms of neo-liberal governance lead to a variety of unforeseen and diverse consequences. This dissertation attempts to understand the impact of these political transformations on household vulnerability in the context of hazardous events called zud. I …

The Effect Of Student Mobility On Student Achievement, Lisa Eddy

University of Kentucky Doctoral Dissertations

Student mobility and its relationship to academic success have been researched since World War II with varied findings (Goebel, 1978). Establishing the relationship between mobility and achievement is difficult due to the fact that mobility is related to many factors. Mobility has been found to be prevalent among students who traditionally demonstrate achievement gaps (specifically students of low-income status) (Long, 1992; Smith, Fien & Paine, 2008).

Mobility’s relationship to achievement is complex. Led by a single definition of mobility, admittance to more than one school in the given district over the period of one academic year, this research study sought …

What Now? What Next? A Narrative Analysis Of Cross-Cultural Adaptation And College Student Retention, Jason Matthews Martin

University of Kentucky Doctoral Dissertations

First year college student retention is important to colleges and universities nationwide (Bean, 2005). Most of the research on retention focuses on self-report data collected from students after they withdraw from the institution. The present study focuses, instead, on student stories about school, as well as at and about “home” during their first semester.

The experiences of students who transition from high school to college are sometimes likened to those of individuals who enter a new culture for the first time. Thus, this dissertation is grounded in cross-cultural adaptation theory (Kim, 1988, 2001), which posits that successful adaptation occurs via …

Engineering Proteins With Unique Characteristics For Diagnostics And Biosensors, Smita Joel

University of Kentucky Doctoral Dissertations

Proteins possess a broad range of structural and functional properties and, therefore, can be employed in a variety of biomedical applications. While a good number of protein-based biosensing systems and biosensors for target analytes have been developed, the search for versatile, highly sensitive and selective sensors with long term stability able to provide fast detection of target analytes continues to be a challenge. To that end, we now report the design and development of modified proteins with tailored characteristics and their further utilization in the development of biosensing systems.

We take advantage of binding proteins that undergo a change in …

The Identity Development Of Preservice Teachers Of Literacy In Field Experiences Considering Their Prior Knowledge, Lindsay Pearle Grow

University of Kentucky Doctoral Dissertations

This qualitative multiple case study explored the identity development of three preservice teachers of literacy. The study focused on the prior knowledge of the preservice teachers of literacy and how their knowledge related to their identity development while in field experiences. The primary question that guided this study was: What is the nature of the construction of identity during field experiences for preservice teachers of literacy? Sub questions explored identity in field experiences and the role of prior pedagogical content knowledge to identity development.

Findings indicated that an evolving habitus central to their identity as literacy teachers could be deduced …

Studies On Silicon Nmr Characterization And Kinetic Modeling Of The Structural Evolution Of Siloxane-Based Materials And Their Applications In Drug Delivery And Adsorption, Jyotrhirmai Ambati

University of Kentucky Doctoral Dissertations

This dissertation presents studies of the synthetic processes and applications of siloxane-based materials. Kinetic investigations of bridged organoalkoxysilanes that are precursors to organic-inorganic hybrid polysilsesquioxanes are a primary focus. Quick gelation despite extensive cyclization is found during the polymerization of bridged silane precursors except for silanes with certain short bridges. This work is an attempt to characterize and understand some of the distinct features of bridged silanes using experimental characterization, kinetic modeling and simulation. In addition to this, the dissertation shows how the properties of siloxane- materials can be engineered for drug delivery and adsorption.

The phase behavior of polymerizing …