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Newcastle Disease Virus Expressing A Dendritic Cell-Targeted Hiv Gag Protein Induces A Potent Gag-Specific Immune Response In Mice, Jad Mammary, Frida Array, Qinshan Gao
Newcastle Disease Virus Expressing A Dendritic Cell-Targeted Hiv Gag Protein Induces A Potent Gag-Specific Immune Response In Mice, Jad Mammary, Frida Array, Qinshan Gao
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Viral vaccine vectors have emerged as an attractive strategy for the development of a human immunodeficiency virus (HIV) vaccine. Recombinant Newcastle disease virus (rNDV) stands out as a vaccine vector since it has a proven safety profile in humans, it is a potent inducer of both alpha interferon (IFN-α) and IFN-β) production, and it is a potent inducer of dendritic cell (DC) maturation. Our group has previously generated an rNDV vector expressing a codon-optimized HIV Gag protein and demonstrated its ability to induce a Gag-specific CD8+ T cell response in mice. In this report we demonstrate that the Gag-specific …
Dendritic Cells Induce Antigen-Specific Regulatory T Cells That Prevent Graft Versus Host Disease And Persist In Mice, Uri Sela, Peter Olds, Andrew Park
Dendritic Cells Induce Antigen-Specific Regulatory T Cells That Prevent Graft Versus Host Disease And Persist In Mice, Uri Sela, Peter Olds, Andrew Park
Publications
Foxp3 + regulatory T cells (T reg cells) effectively suppress immunity, but it is not determined if antigen-induced T reg cells (iT reg cells) are able to persist under conditions of inflammation and to stably express the transcription factor Foxp3. We used spleen cells to stimulate the mixed leukocyte reaction (MLR) in the presence of transforming growth factor β (TGF-β) and retinoic acid. We found that the CD11c high dendritic cell fraction was the most potent at inducing high numbers of alloreactive Foxp3 + cells. The induced CD4 +CD25 +Foxp3 + cells appeared after extensive proliferation. When purified …