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Complete Genome Sequences Of Chop, Delrio, And Grandslam, Three Gordonia Phages Isolated From Soil In Central Arkansas, Heidi N. Mathes, Elijah I. Christenson, John H. Crum, Emme M. Edmondson, Kassidy E. Gray, Luke W. Lawson, Lauren E. Lee, Michael P. Lee, Jackson A. Lipscomb, Morgan E. Masengale, Hannah G. Matthews, Charles M. Mcclain 4th, Tuesday N. Melton, Trace H. Morrow, Alexis M. Perry, David R. Rainwater, Grace E. Renois, Maryann F. Rettig, Duncan C. Troup, Allie J. Wilson, Nathan Reyna, Ruth Plymale

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Chop, DelRio, and GrandSlam are phage with a Siphoviridae morphotype isolated from soil in Arkansas using the host Gordonia terrae 3612. All three are temperate, and their genomes share at least 96% nucleotide identity. These phage are assigned to cluster DI based on gene content similarity to other sequenced actinobacteriophage.

Caddo Nation Chemistry: Art, Commerce, Pottery, And Tools, Joe Jeffers

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The Caddo Nation grew out of the Mississippian culture, the mound builders found throughout what is now the American Southeast and into the Midwest. By 2000 BC, the Caddo or their progenitors had moved up the Mississippi River tributaries as moisture drew them westward. They stopped short of the Great Plains and remained in forested areas. They were primarily hunter-gatherers until 500 BC when Mesoamerican horticultural practices allowed them to establish permanent villages. They raised corn, beans, squash, pumpkins, watermelons, sunflowers, and tobacco. They gathered nuts, berries, roots, and seeds. They continued to fish and hunt deer, bear buffalo, and …

From Genetics To Biotechnology: Synthetic Biology As A Flexible Course-Embedded Research Experience, Kristen C. Johnson, Jamie L. Sabel, Judith Cole, Christin Pruett, Ruth Plymale, Nathan S. Reyna

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The need for changing how science is taught and the expansion of undergraduate research experiences is essential to foster critical thinking in the Natural Sciences. Most faculty research programs only involve a small number of upper-level undergraduate students each semester. The course-based undergraduate research experience (CURE) model enables more students to take ownership over an independent project and experience authentic research. Further, by creating projects that fit into a curriculum's learning goals and student-oriented outcomes, departments help strengthen critical thinking skills in the classroom. Here, we report on the incorporation of a synthetic biology CURE into a mid-level cellular biology …

Twitter: More Than Tweets For Undergraduate Student Researchers, Nathan S. Reyna, Christin Pruett, Mike Morrison, Jennifer Fowler, Sumali Pandey, Lori Hensley

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During the COVID-19 pandemic, biology educators were forced to think of ways to communicate with their students, engaging them in science and with the scientific community. For educators using course-based undergraduate research experiences (CUREs), the challenge to have students perform real science, analyze their work, and present their results to a larger scientific audience was difficult as the world moved online. Many instructors were able to adapt CUREs utilizing online data analysis and virtual meeting software for class discussions and synchronous learning. However, interaction with the larger scientific community, an integral component of making science relevant for students and allowing …

Circulating Exosomal Micrornas As Predictive Biomarkers Of Neoadjuvant Chemotherapy Response In Breast Cancer, Valentina K. Todorova, Stephanie D. Byrum, Allen J. Gies, Cade Haynie, Hunter Smith, Nathan S. Reyna, Issam Makhoul

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Background: Neoadjuvant chemotherapy (NACT) is an increasingly used approach for treatment of breast cancer. The pathological complete response (pCR) is considered a good predictor of disease-specific survival. This study investigated whether circulating exosomal microRNAs could predict pCR in breast cancer patients treated with NACT. Method: Plasma samples of 20 breast cancer patients treated with NACT were collected prior to and after the first cycle. RNA sequencing was used to determine microRNA profiling. The Cancer Genome Atlas (TCGA) was used to explore the expression patterns and survivability of the candidate miRNAs, and their potential targets based on the expression levels and …

Complete Genome Sequences Of Four Putatively Antibiotic-Producing Bacteria Isolated From Soil In Arkansas, Usa, Ruth Plymale, Griffin Hopkins, Taylor Johnson, Taylor Savage, Danielle Schaal

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Soil bacteria can be a valuable source of antimicrobial compounds. Here, we report the complete genomes of four soil bacteria that were isolated by undergraduate microbiology students as part of a course-based research experience. These genomes were assembled using a hybrid approach combining paired-end Illumina reads with Oxford Nanopore Technologies MinION reads.

An Employment-Weighted Municipal Stock Index, Chris Brune, James Files, Phil Rice

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State and local officials are often tasked with policy decisions that are influenced by projections of future economic conditions. However, properly assessing and predicting local economic performance is challenging. Common macroeconomic indicators can be helpful, but additional tools are needed. Stock indices have traditionally been used in the investment community to evaluate performance. While a recent surge in fundamental indexing has led to a debate over performance measurement, other uses of fundamental indices have been largely ignored. We introduce an employment-weighted stock index as a supplement to traditional forecasting measures for policymakers at the state and local levels

History Of The Pruet School Of Christian Studies/Department Of Religion At Ouachita Baptist University/Ouachita Baptist College (1886-2021), J. Daniel Hayes

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This is a short history of the Religion Department/School of Christian Studies at Ouachita Baptist University. This document includes a historical overview, faculty publications, the theological and educational philosophy of the school, history of Bible-related CORE courses, curriculum, and lists of faculty/staff with some biographical information.

Transitioning Cell Culture Cure Labs From Campus To Online: Novel Strategies For A Novel Time, Jaime L. Sabel, Kendra Wright, Jacob J. Adler, Gary Bates, La Shall Bates, Sumali Pandey, Amanda M. Simons, Sarah J. Swerdlow, Nathan S. Reyna, Lori Hensley

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Course-based undergraduate research experiences (CUREs) provide a way for students to gain research experience in a classroom setting. Few examples of cell culture CUREs or online CUREs exist in the literature. The Cell Biology Education Consortium (CBEC) provides a network and resources for instructors working to incorporate cell-culture based research into the classroom. In this article, we provide examples from six instructors from the CBEC network on how they structure their cell-culture CUREs and how they transitioned the labs to online in the spring semester of 2020. We intend for these examples to provide instructors with ideas for strategies to …

Gene Expression And Data Analysis Pipeline Using Cancer Bioportal In The Classroom, Chassidy N. Barnes, Blake P. Johnson, Stefanie W. Leacock, Ruben M. Ceballos, Lori L. Hensley, Nathan S. Reyna

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At institutions with an emphasis on authentic research experiences as an integral part of the biology curriculum, COVID created a huge challenge for course instructors whose learning objectives were designed for such experiences. Moving such laboratory experiences online when remote learning became necessary has resulted in a new model for CUREs that utilizes free online databases to provide not only a novel research experience for students, but also the opportunity to engage in big data analysis. Cancer BioPortal (cBioPortal) is an open-access collective cancer research resource for storing and exploring clinical, genomic, proteomic, and transcriptomic data. cBioPortal eliminates the computational …

Novel Role For The Golgi Membrane Protein Tmem165 In Control Of Migration And Invasion For Breast Carcinoma, Pavitra Murali, Blake P. Johnson, Zhongpeng Lu, Leslie Climer, Danielle A. Scott, Francois Foulquier, Gabriela Oprea-Ilies, Vladimir Lupashin, Richard R. Drake, Karen L. Abbott

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The TMEM165 gene encodes for a multiple pass membrane protein localized in the Golgi that has been linked to congenital disorders of glycosylation. The TMEM165 protein is a putative ion transporter that regulates H+/Ca++/Mn++ homeostasis and pH in the Golgi. Previously, we identified TMEM165 as a potential biomarker for breast carcinoma in a glycoproteomic study using late stage invasive ductal carcinoma tissues with patient-matched adjacent normal tissues. The TMEM165 protein was not detected in non-malignant matched breast tissues and was detected in invasive ductal breast carcinoma tissues by mass spectrometry. Our hypothesis is that the TMEM165 protein confers a growth …

Glioma-Derived Exosomes Drive The Differentiation Of Neural Stem Cells To Astrocytes, Krishna D. Sharma, Danielle Schaal, Rajshekhar A. Kore, Rabab N. Hamzah, Sahitya Chetan Pandanaboina, Abdallah Hayar, Robert J. Griffin, Malathi Srivatsan, Nathan S. Reyna, Jennifer Yanhua Xie

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Exosomes appear to be effective inter-cellular communicators delivering several types of molecules, such as proteins and RNAs, suggesting that they could influence neural stem cell (NSC) differentiation. Our RNA sequencing studies demonstrated that the RNAs related to cell proliferation and astrocyte differentiation were upregulated in human mesenchymal stem cells (hMSC) when co-cultured with exosomes obtained from the culture medium of human glioma cells (U87). Metallothionein 3 and elastin genes, which are related to cell proliferation, increased 10 and 7.2 fold, respectively. Expression of genes for astrocyte differentiation, such as tumor growth factor alpha, induced protein 3 of the NOTCH1 family, …

Genomic Diversity Of Bacteriophages Infecting Microbacterium Spp, Deborah Jacobs-Sera, Nathan S. Reyna, Lawrence A. Abad, Richard M. Alvey, Kirk R. Anders, Haley G. Aull, Suparna S. Bhalla, Lawrence S. Blumer, David W. Bollivar, J. Alfred Bonilla, Kristen A. Butela, Roy J. Coomans, Steven G. Cresawn, Tom D'Elia, Arturo Diaz, Ashley M. Divens, Nicholas P. Edgington, Gregory D. Frederick, Maria D. Gainey, Rebecca A. Garlena, Kenneth W. Grant, Susan M.R. Gurney, Heather L. Hendrickson, Lee E. Hughes, Margaret A. Kenna, Karen K. Klyczek, Hari Kotturi, Travis N. Mavrich, Angela L. Mckinney, Evan C. Merkhofer, Jordan Moberg Parker, Sally D. Molloy, Denise L. Monti, Dana A. Pape-Zambito

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The bacteriophage population is vast, dynamic, old, and genetically diverse. The genomics of phages that infect bacterial hosts in the phylum Actinobacteria show them to not only be diverse but also pervasively mosaic, and replete with genes of unknown function. To further explore this broad group of bacteriophages, we describe here the isolation and genomic characterization of 116 phages that infect Microbacterium spp. Most of the phages are lytic, and can be grouped into twelve clusters according to their overall relatedness; seven of the phages are singletons with no close relatives. Genome sizes vary from 17.3 kbp to 97.7 kbp, …

Teaching Business Statistics: Some Useful Relationships, Phil Rice, Chris Brune

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The purpose of this paper is to suggest an instructional approach in the introductory business statistics course that utilizes relationships between separately introduced topics. The paper will explore three “useful relationships” that can assist classroom instruction: (1) the relationship between the simple arithmetic mean, the weighted arithmetic mean, and the expected value of a discrete probability distribution; (2) the relationship between the use of the multiplication rule to calculate the joint probability associated with two events, use of tree diagrams, and the use of the binomial and hypergeometric distributions; and (3) the relationship between the geometric mean and the compound …

Life In The Time Of Covid-19, Joe Jeffers

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This narrative is a personal account of the COVID-19 pandemic and its effect on my life in 2020. Three factors come to bear on my reactions. First, I am 75 years old. Second, I have a minor heart condition. Third, I am a scientist. The first two put me in a higher risk category, and my behavior changed accordingly. The third is the window through which I view the world. It affects my reaction to data as the nature of SARS-CoV-2 and COVID-19 are revealed. I follow numerous information sources about the pandemic and share those articles on Facebook. Retirement …

Generation Of A Fully Human Scfv That Binds Tumor-Specific Glycoforms, Zhongpeng Lu, Kalika Kamat, Blake P. Johnson, Catherin C. Yin, Nathalie Scholler, Karen L. Abbott

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Tumor-specific glycosylation changes are an attractive target for the development of diagnostic and therapeutic applications. Periostin is a glycoprotein with high expression in many tumors of epithelial origin including ovarian cancer. Strategies to target the peptide portion of periostin as a diagnostic or therapeutic biomarker for cancer are limited due to increased expression of periostin in non-cancerous inflammatory conditions. Here, we have screened for antibody fragments that recognize the tumor-specific glycosylation present on glycoforms of periostin containing bisecting N-glycans in ovarian cancer using a yeast-display library of antibody fragments, while subtracting those that bind to the periostin protein with glycoforms …

French Place Names In Clark County, Arkansas, Joe Jeffers

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French place names are common in Arkansas, especially in south Arkansas, where after the French explorers left, French trappers and settlers from Canada moved in. Some of those names remain unchanged from the original French. General usage and English speaking settlers modified others. Clark County was one of five counties established in the Arkansas Territory. Its boundaries changed five times before reaching its present form in 1877. This article explores French place names in today’s Clark County and in the original Clark County formed in 1818.

A Geological History Of Clark County, Arkansas, Joe Jeffers

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The geological history of Clark County is described ranging from 500 million years ago to the present. One-third of present-day Clark County is in the Ouachita Mountains, two-thirds is in the Coastal Plain. The formation of the Ouachita Mountains is described, as is the cretaceous formations that led to biological fossils. Historic mining of minerals is also discussed. For the more technically inclined, a detailed description of the geological formations of the county is included near the end.

100 Years Ago: Front-Page Stories From Arkadelphia's Southern Standard, Lisa K. Speer

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The following news items were extracted from the front pages of Arkadelphia's Southern Standard weekly newspaper of 1919. The articles illustrate the variety of news published by the paper and offer glimpses into life in early 20th-century Clark County. The return to normalcy following the end of a world war; agricultural and economic development of the county; and moonshining were just a few of the themes that ran through the news that made the front pages of 1919.

College Students' Social Perceptions Toward Individuals With Intellectual Disability, B. Allyson Phillips, Stoni Fortney, Lindsey Swafford

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The purpose of the current study was to describe the social perceptions of American college students towards individuals with intellectual disability (ID), identify factors that influence social perception, and determine if level of functioning alters one’s perception. The sample was comprised of 186 American college students. The participants completed the Attitudes Toward Intellectual Disability Questionnaire (ATTID). The ATTID measures five factors—discomfort towards ID, knowledge of capacity and rights, interaction with individuals with ID, sensibility/tenderness, and knowledge of causes. The students’ overall social perception towards ID was primarily positive for all factors except for sensibility/tenderness. More positive social perception was found …

Hypoxia-Derived Exosomes Induce Putative Altered Pathways In Biosynthesis And Ion Regulatory Channels In Glioblastoma Cells, Rajshekhar A. Kore, Jacob L. Edmondson, Samir V. Jenkins, Azemat Jamshidi-Parsian, Ruud P.M. Dings, Nathan S. Reyna, Robert J. Griffin

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Hypoxia, a hallmark characteristic of glioblastoma (GBM) induces changes in the transcriptome and the proteome of tumor cells. We discovered that hypoxic stress produces significant qualitative and quantitative changes in the protein content of secreted exosomes from GBM cells. Among the proteins found to be selectively elevated in hypoxic exosomes were protein-lysine 6-oxidase (LOX), thrombospondin-1 (TSP1), vascular derived endothelial factor (VEGF) and a disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAMTS1), well studied contributors to tumor progression, metastasis and angiogenesis. Our findings demonstrate that hypoxic exosomes induce differential gene expression in recipient glioma cells. Glioma cells stimulated with hypoxic exosomes …

Mass Spectrometry As A Tool To Enhance “-Omics” Education, Michael J. Wolyniak, Nathan S. Reyna, Ruth Plymale, Welkin H. Pope, Daniel E. Westholm

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The rise of "-omics" related technologies makes it essential for students to have experience working with large bioinformatics data sets. Although”-omic” datasets are complex and abstract, effective instruction can be improved when students see the direct connections between the data on a computer screen and the results of "wet lab" experimentation. Here we describe the use of protein mass spectrometry as a means for students to gain experience in connecting bioinformatic data with work done at the lab bench. Course-based Research Experiences (CREs) based on these techniques are accessible to institutions of all types as a result of rapidly declining …

Contributions Of Women To Clark County's History, Lisa K. Speer

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Excerpted from a lecture given for the Clark County bicentennial, this article focuses on the contributions of Clark County women to the community during the major movements and events of the late 19th and 20th centuries.

Genome Sequences Of Four Cluster P Mycobacteriophages, Erin L. Doyle, Christy L. Fillman, Nathan S. Reyna, Ruth C. Plymale, Deborah M. Tobiason, Daniel E. Westholm, Jonathan L. Askins, Brittany P. Backus, Ashlynn C. Baker, Harrison S. Ballard, Paul J. Bisesi, Logan Bond, Deanna Byrnes, Hannah Carlstedt, Kinnon S. Dodson, Megan J. Fallert, Kyla J. Foster, Daniel N. Games, Tristan R. Grams, Nancy A. Guild, Autumn Hurd, Nicholas Iwata, Cassidy R. Kepler, Lucinda R. Krenzke, Kelly Luekens, Jackie Lewis, Cali Mcentee, Justin C. Mcgee, Noah Nalley, Jade Prochaska, Reid G. Rogers, Jessica B. Schipper, Kelsey Snyder

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Four bacteriophages infecting Mycobacterium smegmatis mc2155 (three belonging to subcluster P1 and one belonging to subcluster P2) were isolated from soil and sequenced. All four phages are similar in the left arm of their genomes, but the P2 phage differs in the right arm. All four genomes contain features of temperate phages.

Exhibit Still Open To The Public, Wendy Bradley Richter

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Time is running out for Clark County area citizens to enjoy a Smithsonian Museum exhibit here in Arkadelphia.

The Smithsonian Institution Traveling Exhibition Service of the Museum on Main Street program and the Arkansas Humanities Council have partnered to bring "Hometown Teams: How Sports Shape America" to Ouachita Baptist University.

The Glycosyltransferase Gnt-Iii Activates Notch Signaling And Drives Stem Cell Expansion To Promote The Growth And Invasion Of Ovarian Cancer, Heba Allam, Blake P. Johnson, Mao Zhang, Zhongpeng Lu, Martin J. Cannon, Karen L. Abbott

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Glycosylation changes associated with cellular transformation can facilitate the growth and progression of tumors. Previously we discovered that the gene Mgat3 encoding the glycosyltransferase GnT-III is elevated in epithelial ovarian carcinomas (EOCs) and leads to the production of abnormal truncated N-linked glycan structures instead of the typical bisected forms. In this study, we are interested in discovering how these abnormal glycans impact the growth and progression of ovarian cancer. We have discovered using stable shRNA gene suppression that GnT-III expression controls the expansion of side-population cells, also known as cancer stem cells. More specifically, we found that GnT-III expression regulates …

Parenting Children With Down Syndrome: An Analysis Of Parenting Styles, Parenting Dimensions, And Parental Stress, B. Allyson Phillips

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Effective parenting is vital for a child’s development. Although much work has been conducted on parenting typically developing children, little work has examined parenting children with Down syndrome.

The purpose of the current study was to compare the parenting styles and dimensions in mothers of children with DS and mothers of TD children.

Thirty-five mothers of children with DS and 47 mothers of TD children completed questionnaires about parenting, parental stress, child behavior problems, and child executive function.

We found that mothers of children with DS use an authoritative parenting style less and a permissive parenting style more than mothers …

Accounting In Fiction, S. Ray Granade

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A bibliography of fiction in which accountants are characters, or in which accountancy plays a part in the plot.

"The Most Patient Of Animals, Next To The Ass:" Jan Smuts, Howard University, And African American Leandership, 1930, Robert Edgar, Myra Ann Houser

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Former South African Prime Minister Jan Smuts’ 1930 European and North American tour included a series of interactions with diasporic African and African American activists and intelligentsia. Among Smuts’s many remarks stands a particular speech he delivered in New York City, when he called Africans “the most patient of all animals, next to the ass.” Naturally, this and other comments touched off a firestorm of controversy surrounding Smuts, his visit, and segregationist South Africa’s laws. Utilizing news coverage, correspondence, and recollections of the trip, this article uses his visit as a lens into both African American relations with Africa and …

Rapid Verification Of Terminators Using The Pgr-Blue Plasmid And Golden Gate Assembly, Jace C. Bradshaw, Allea Belle Gongola, Nathan S. Reyna

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The goal of this protocol is to allow for the rapid verification of bioinformatically identified terminators. Further, the plasmid (pGR-Blue) is designed specifically for this protocol and allows for the quantification of terminator efficiency. As a proof of concept, six terminators were bioinformatically identified in the mycobacteriophage Bernal13. Once identified, terminators were then made as oligonucleotides with the appropriate sticky ends and annealed together. Using Golden Gate Assembly (GGA), terminators were then cloned into pGR-Blue. Under visible light, false positive colonies appear blue and positively transformed colonies are white/yellow. After induction of an arabinose inducible promoter (pBad) with arabinose, colony …