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Identification Of A Short Cell-Penetrating Peptide From Bovine Lactoferricin For Intracellular Delivery Of Dna In Human A549 Cells, Betty Revon Liu, Yue-Wern Huang, Robert Aronstam, Han-Jung Lee
Identification Of A Short Cell-Penetrating Peptide From Bovine Lactoferricin For Intracellular Delivery Of Dna In Human A549 Cells, Betty Revon Liu, Yue-Wern Huang, Robert Aronstam, Han-Jung Lee
Biological Sciences Faculty Research & Creative Works
Cell-penetrating peptides (CPPs) have been shown to deliver cargos, including protein, DNA, RNA, and nanomaterials, in fully active forms into live cells. Most of the CPP sequences in use today are based on non-native proteins that may be immunogenic. Here we demonstrate that the L5a CPP (RRWQW) from bovine lactoferricin (LFcin), stably and noncovalently complexed with plasmid DNA and prepared at an optimal nitrogen/phosphate ratio of 12, is able to efficiently enter into human lung cancer A549 cells. The L5a CPP delivered a plasmid containing the enhanced green fluorescent protein (EGFP) coding sequence that was subsequently expressed in …
Foraging Alters Resilience/Vulnerability To Sleep Disruption And Starvation In Drosophila, Jeffrey Donlea, Averi Leahy, Matthew S. Thimgan, Yasuko Suzuki, Bryon N. Hughson, Marla B. Sokolowski, Paul J. Shaw
Foraging Alters Resilience/Vulnerability To Sleep Disruption And Starvation In Drosophila, Jeffrey Donlea, Averi Leahy, Matthew S. Thimgan, Yasuko Suzuki, Bryon N. Hughson, Marla B. Sokolowski, Paul J. Shaw
Biological Sciences Faculty Research & Creative Works
Recent human studies suggest that genetic polymorphisms allow an individual to maintain optimal cognitive functioning during sleep deprivation. If such polymorphisms were not associated with additional costs, selective pressures would allow these alleles to spread through the population such that an evolutionary alternative to sleep would emerge. To determine whether there are indeed costs associated with resiliency to sleep loss, we challenged natural allelic variants of the foraging gene (for) with either sleep deprivation or starvation. Flies with high levels of Protein Kinase G (PKG) (forR) do not display deficits in short-term memory following 12 …
Toxicity Of Transition Metal Oxide Nanoparticles: Recent Insights From In Vitro Studies, Yue-Wern Huang, Chi-Heng Wu, Robert Aronstam
Toxicity Of Transition Metal Oxide Nanoparticles: Recent Insights From In Vitro Studies, Yue-Wern Huang, Chi-Heng Wu, Robert Aronstam
Biological Sciences Faculty Research & Creative Works
Nanotechnology has evolved to play a prominent role in our economy. Increased use of nanomaterials poses potential human health risk. It is therefore critical to understand the nature and origin of the toxicity imposed by nanomaterials (nanotoxicity). In this article we review the toxicity of the transition metal oxides in the 4th period that are widely used in industry and biotechnology. Nanoparticle toxicity is compellingly related to oxidative stress and alteration of calcium homeostasis, gene expression, pro-inflammatory responses, and cellular signaling events. The precise physicochemical properties that dictate the toxicity of nanoparticles have yet to be defined, but may include …
The Multiple Roles Of Cyk1p In The Assembly And Function Of The Actomyosin Ring In Budding Yeast, Katie Shannon, Rong Li
The Multiple Roles Of Cyk1p In The Assembly And Function Of The Actomyosin Ring In Budding Yeast, Katie Shannon, Rong Li
Biological Sciences Faculty Research & Creative Works
The budding yeast IQGAP-like protein Cyk1p/Iqg1p localizes to the mother-bud junction during anaphase and has been shown to be required for the completion of cytokinesis. In this study, video microscopy analysis of cells expressing green fluorescent protein-tagged Cyk1p/Iqg1p demonstrates that Cyk1p/Iqg1p is a dynamic component of the contractile ring during cytokinesis. Furthermore, in the absence of Cyk1p/Iqg1p, myosin II fails to undergo the contraction-like size change at the end of mitosis. To understand the mechanistic role of Cyk1p/Iqg1p in actomyosin ring assembly and dynamics, we have investigated the role of the structural domains that Cyk1p/Iqg1p shares with IQGAPs. An amino …