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Natural Hyperthermia And Expression Of The Heat Shock Protein Hsp70 Affect Developmental Abnormalities In Drosophila Melanogaster, Stephen P. Roberts, Martin E. Feder Nov 1999

Natural Hyperthermia And Expression Of The Heat Shock Protein Hsp70 Affect Developmental Abnormalities In Drosophila Melanogaster, Stephen P. Roberts, Martin E. Feder

Biological Sciences Faculty Research & Creative Works

We Demonstrate that Natural Heat Stress on Wild Larval Drosophila Melanogaster Results in Severe Developmental Defects in >10% of Eclosing Adults, and that Increased Copy Number of the Gene Encoding the Major Inducible Heat Shock Protein of D. Melanogaster, Hsp70, is Sufficient to Reduce the Incidence of Such Abnormalities. Specifically, Non-Adult D. Melanogaster Inhabiting Necrotic Fruit Experienced Severe, Often Lethal Heat Stress in Natural Settings. Adult Flies Eclosing from Wild Larvae that Had Survived Natural Heat Stress Exhibited Severe Developmental Anomalies of Wing and Abdominal Morphology, Which Should Dramatically Affect Fitness. the Frequency of Developmental Abnormalities Varied Along Two Independent …


Mechanisms Of Thermal Stability During Flight In The Honeybee Apis Mellifera, Stephen P. Roberts, Jon F. Harrison Jun 1999

Mechanisms Of Thermal Stability During Flight In The Honeybee Apis Mellifera, Stephen P. Roberts, Jon F. Harrison

Biological Sciences Faculty Research & Creative Works

Thermoregulation of the Thorax Allows Honeybees (Apis Mellifera) to Maintain the Flight Muscle Temperatures Necessary to Meet the Power Requirements for Flight and to Remain Active Outside the Hive Across a Wide Range of Air Temperatures (T(A)). to Determine the Heat-Exchange Pathways through Which Flying Honeybees Achieve Thermal Stability, We Measured Body Temperatures and Rates of Carbon Dioxide Production and Water Vapor Loss between T(A) Values of 21 and 45°C for Honeybees Flying in a Respirometry Chamber. Body Temperatures Were Not Significantly Affected by Continuous Flight Duration in the Respirometer, indicating that Flying Bees Were at Thermal Equilibrium. Thorax Temperatures …


The Multiple Roles Of Cyk1p In The Assembly And Function Of The Actomyosin Ring In Budding Yeast, Katie Shannon, Rong Li Feb 1999

The Multiple Roles Of Cyk1p In The Assembly And Function Of The Actomyosin Ring In Budding Yeast, Katie Shannon, Rong Li

Biological Sciences Faculty Research & Creative Works

The budding yeast IQGAP-like protein Cyk1p/Iqg1p localizes to the mother-bud junction during anaphase and has been shown to be required for the completion of cytokinesis. In this study, video microscopy analysis of cells expressing green fluorescent protein-tagged Cyk1p/Iqg1p demonstrates that Cyk1p/Iqg1p is a dynamic component of the contractile ring during cytokinesis. Furthermore, in the absence of Cyk1p/Iqg1p, myosin II fails to undergo the contraction-like size change at the end of mitosis. To understand the mechanistic role of Cyk1p/Iqg1p in actomyosin ring assembly and dynamics, we have investigated the role of the structural domains that Cyk1p/Iqg1p shares with IQGAPs. An amino …