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Oxidative Stress Suppresses The Heteromeric Kir4.1-Kir5.1 Channel Via S-Glutathionylation, Yang Wu
Oxidative Stress Suppresses The Heteromeric Kir4.1-Kir5.1 Channel Via S-Glutathionylation, Yang Wu
Biology Theses
Potassium transport channels regulate cellular excitability and membrane potentials. Dysfunction of potassium channels may happen under certain pathophysiological conditions via post-translational modification (PTM), inducing the S-glutathionylation, in oxidative stress. Here, we try to demonstrate the effect of ROS on Kir4.1 and Kir5.1 channel. It is found that the target subunit of ROS is Kir5.1 instead of Kir4.1 subunit. Kir4.1-Kir5.1 heteromeric channel is inhibited by different kinds of oxidants. The patch clamp study confirmed that these oxidants work on the intracellular side of the Kir5.1 subunit. Further experiments demonstrate that the inhibition of Kir4.1-Kir5.1 channels is through S-glutathionylation of the Kir5.1 …