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Effects Of Escapin Intermediate Products (Eip-K) On Biofilms Of Pseudomonas Aeruginosa, Marwa Nabil Abdelaziz Ahmed

Biology Theses

Escapin is an L-amino acid oxidase that produces antimicrobial metabolites collectively called “Escapin Intermediate Products” (EIP-K). EIP-K and H₂O₂ together were previously shown to be bactericidal towards diverse planktonic bacteria. The present work investigates the ability of EIP-K and H₂O₂ to antagonize bacterial biofilms, using Pseudomonas aeruginosa as a model. The project had three aims: 1) determine the most effective concentrations of EIP-K and H₂O₂ necessary to break down existing P. aeruginosa biofilms, using a crystal violet assay; 2) examine the ability of EIP-K + H₂O₂ to inhibit …