Open Access. Powered by Scholars. Published by Universities.®
- Discipline
-
- Physical Sciences and Mathematics (38)
- Computer Sciences (20)
- Medicine and Health Sciences (17)
- Life Sciences (16)
- Medical Sciences (16)
-
- Astrophysics and Astronomy (15)
- Medical Microbiology (11)
- Biology (7)
- Infectious Disease (7)
- Medical Specialties (7)
- Diseases (6)
- External Galaxies (5)
- Biochemistry, Biophysics, and Structural Biology (4)
- Medical Immunology (4)
- Parasitic Diseases (4)
- Stars, Interstellar Medium and the Galaxy (4)
- Bacteriology (3)
- Cell and Developmental Biology (3)
- Medical Biochemistry (3)
- Microbiology (3)
- Social and Behavioral Sciences (3)
- Biochemistry (2)
- Cell Biology (2)
- Cosmology, Relativity, and Gravity (2)
- Economics (2)
- Genetics and Genomics (2)
- Medical Cell Biology (2)
- Medical Genetics (2)
- Medical Pathology (2)
- Physics (2)
- Keyword
-
- Animals (4)
- Carrier proteins (3)
- Distances and redshifts (3)
- Elliptical and lenticular (3)
- Galaxies (3)
-
- Membrane proteins (3)
- Mobile computing (3)
- Mobile-agent (3)
- Protein binding (3)
- Amino acid sequence (2)
- Antibodies (2)
- Astrophysics (2)
- Binding sites (2)
- Cd (2)
- Clusters (2)
- Female (2)
- Fungal proteins (2)
- Genetics (2)
- Metabolism (2)
- Physics (2)
- Saccharomyces cerevisiae (2)
- AIDS (1)
- Adaptor proteins (1)
- Affinity (1)
- Alleles (1)
- Amino acid substitution (1)
- Analysis (1)
- Animal (1)
- Animals apoptosis caenorhabditis elegans/ cytology germ cells/ cytology homeostasis research support (1)
- Antisense (1)
- Publication
- Publication Type
Articles 1 - 30 of 73
Full-Text Articles in Entire DC Network
Structure And Function Analysis Of Lin-14, A Temporal Regulator Of Postembryonic Developmental Events In Caenorhabditis Elegans, Yang Hong, Rosalind C. Lee, Victor Ambros
Structure And Function Analysis Of Lin-14, A Temporal Regulator Of Postembryonic Developmental Events In Caenorhabditis Elegans, Yang Hong, Rosalind C. Lee, Victor Ambros
Dartmouth Scholarship
During postembryonic development of Caenorhabditis elegans, the heterochronic gene lin-14 controls the timing of developmental events in diverse cell types. Three alternativelin-14 transcripts are predicted to encode isoforms of a novel nuclear protein that differ in their amino-terminal domains. In this paper, we report that the alternative amino-terminal domains of LIN-14 are dispensable and that a carboxy-terminal region within exons 9 to 13 is necessary and sufficient for in vivo LIN-14 function. A transgene capable of expressing only one of the three alternativelin-14 gene products rescues a lin-14 null mutation and is developmentally regulated by lin-4. …
Fundamental Plane Distances To Early-Type Field Galaxies In The South Equatorial Strip. I. The Spectroscopic Data, K. R. Müller, G. Wegner, S. Raychaudhury, W. Freudling
Fundamental Plane Distances To Early-Type Field Galaxies In The South Equatorial Strip. I. The Spectroscopic Data, K. R. Müller, G. Wegner, S. Raychaudhury, W. Freudling
Dartmouth Scholarship
Radial velocities and central velocity dispersions are derived for 238 E/S0 galaxies from medium-resolution spectroscopy. New spectroscopic data have been obtained as part of a study of the Fundamental Plane distances and peculiar motions of early-type galaxies in three selected directions of the South Equatorial Strip, undertaken in order to investigate the reality of large-scale streaming motion; results of this study have been reported in M\"uller $et$ $al.$ (1998). The new APM South Equatorial Strip Catalog (−17.5 < δ < +2.5) was used to select the sample of field galaxies in three directions: (1) 15h10 – 16h10; (2) 20h30 – 21h50; (3) 00h10 – 01h30. The spectra obtained have a median S/N per ̊A of 23, an in- strumental resolution (FWHM) of ∼ 4 ̊A, and the spectro- graph resolution (dispersion) is ∼ 100 km s−1. The Fourier cross-correlation method was used to derive the radial ve- locities and velocity dispersions. The velocity dispersions have been corrected for the size of the aperture and for the galaxy effective radius. Comparisons of the derived radial velocities with data from the literature show that our values are accurate to 40 km s−1. A comparison with results from Jørgensen et al. (1995) shows that the derived central velocity dispersion have an rms scatter of 0.036 in log σ. There is no offset relative to the velocity dispersions of Davies et al. (1987).
Peculiar Velocities Of Nonlinear Structure: Voids In Mcvittie Spacetime, Sakai, Nobuyuki, Haines, Paul
Peculiar Velocities Of Nonlinear Structure: Voids In Mcvittie Spacetime, Sakai, Nobuyuki, Haines, Paul
Dartmouth Scholarship
As a study of peculiar velocities of nonlinear structure, we analyze the model of a relativistic thin-shell void in the expanding universe. (1) Adopting McVittie (MV) spacetime as a background universe, we investigate the dynamics of an uncompensated void with negative MV mass. Although the motion itself is quite different from that of a compensated void, as shown by Haines & Harris (1993), the present peculiar velocities are not affected by MV mass. (2) We discuss how precisely the formula in the linear perturbation theory applies to nonlinear relativistic voids, using the results in (1) as well as the previous …
A Ligand Binding Domain Mutation In The Mouse Glucocorticoid Receptor Functionally Links Chromatin Remodeling And Transcription Initiation, Lynn A. Sheldon, Catharine L. Smith, Jack E. Bodwell, Allan U. Munck, Gordon L. Hager
A Ligand Binding Domain Mutation In The Mouse Glucocorticoid Receptor Functionally Links Chromatin Remodeling And Transcription Initiation, Lynn A. Sheldon, Catharine L. Smith, Jack E. Bodwell, Allan U. Munck, Gordon L. Hager
Dartmouth Scholarship
We utilized the mouse mammary tumor virus (MMTV) long terminal repeat (LTR) in vivo to understand how the interaction of the glucocorticoid receptor (GR) with a nucleosome-assembled promoter allows access of factors required for the transition from a repressed promoter to a derepressed, transcriptionally competent promoter. A mutation (C644G) in the ligand binding domain (LBD) of the mouse GR has provided information regarding the steps required in the derepression/activation process and in the functional significance of the two major transcriptional activation domains, AF1 and AF2. The mutant GR activates transcription from a transiently transfected promoter that has a disordered nucleosomal …
A Pdz-Interacting Domain In Cftr Is An Apical Membrane Polarization Signal, Bryan D. Moyer, Jerod Denton, Katherine H. Karlson, Donna Reynolds, Shusheng Wang, John E. Mickle, Michael Milewski, Garry R. Cutting, William B. Guggino, Min Li, Bruce A. Stanton
A Pdz-Interacting Domain In Cftr Is An Apical Membrane Polarization Signal, Bryan D. Moyer, Jerod Denton, Katherine H. Karlson, Donna Reynolds, Shusheng Wang, John E. Mickle, Michael Milewski, Garry R. Cutting, William B. Guggino, Min Li, Bruce A. Stanton
Dartmouth Scholarship
Polarization of the cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-activated chloride channel, to the apical plasma membrane of epithelial cells is critical for vectorial transport of chloride in a variety of epithelia, including the airway, pancreas, intestine, and kidney. However, the motifs that localize CFTR to the apical membrane are unknown. We report that the last 3 amino acids in the COOH-terminus of CFTR (T-R-L) comprise a PDZ-interacting domain that is required for the polarization of CFTR to the apical plasma membrane in human airway and kidney epithelial cells. In addition, the CFTR mutant, S1455X, which lacks the 26 …
Sec34p, A Protein Required For Vesicle Tethering To The Yeast Golgi Apparatus, Is In A Complex With Sec35p, Susan M. Vanrheenen, Xiaochun Cao, Stephanie K. Sapperstein, Elbert C. Chiang, Vladimir V. Lupashin, Charles Barlowe, M. Gerard Waters
Sec34p, A Protein Required For Vesicle Tethering To The Yeast Golgi Apparatus, Is In A Complex With Sec35p, Susan M. Vanrheenen, Xiaochun Cao, Stephanie K. Sapperstein, Elbert C. Chiang, Vladimir V. Lupashin, Charles Barlowe, M. Gerard Waters
Dartmouth Scholarship
A screen for mutants of Saccharomyces cerevisiae secretory pathway components previously yielded sec34, a mutant that accumulates numerous vesicles and fails to transport proteins from the ER to the Golgi complex at the restrictive temperature (Wuestehube, L.J., R. Duden, A. Eun, S. Hamamoto, P. Korn, R. Ram, and R. Schekman. 1996. Genetics. 142:393–406). We find that SEC34 encodes a novel protein of 93-kD, peripherally associated with membranes. The temperature-sensitive phenotype of sec34-2 is suppressed by the rab GTPase Ypt1p that functions early in the secretory pathway, or by the dominant form of the ER to Golgi complex target-SNARE …
An Access-Control Calculus For Spanning Administrative Domains, Jon Howell, David Kotz
An Access-Control Calculus For Spanning Administrative Domains, Jon Howell, David Kotz
Computer Science Technical Reports
In our quest to give users uniform access to resources unimpeded by administrative boundaries, we discovered that we needed transitive sharing among users, with the possibility of restricted access along each sharing link. To achieve that goal, we extend Lampson et al.'s calculus for access control to support restricted delegations. We discuss the advantages of our extension, including the simplification of constructs like ACLs and statement expiration. We also apply our extension to model the Simple Public Key Infrastructure and make suggestions about its future development. Our extended calculus exposes some surprising consequences in such systems that use restricted delegation.
The Pulsation Properties Of Procyon A, Brian Chaboyer, P. Demarque, D. B. Guenther
The Pulsation Properties Of Procyon A, Brian Chaboyer, P. Demarque, D. B. Guenther
Dartmouth Scholarship
A grid of stellar evolution models for Procyon A has been calculated. These models include the best physics available to us (including the latest opacities and equation of state) and are based on the revised astrometric mass of Girard et al. Models were calculated with helium diffusion and with the combined effects of helium and heavy-element diffusion. Oscillation frequencies for l = 0, 1, 2, and 3 p-modes and the characteristic period spacing for the g-modes were calculated for these models. We find that g-modes are sensitive to model parameters that effect the structure of the core, …
Immune Cd8+ T Cells Prevent Reactivation Of Toxoplasma Gondii Infection In The Immunocompromised Host, Imtiaz A. Khan, William R. Green, Lloyd H. Kasper, Kathy A. Green, Joseph D. Schwartzman
Immune Cd8+ T Cells Prevent Reactivation Of Toxoplasma Gondii Infection In The Immunocompromised Host, Imtiaz A. Khan, William R. Green, Lloyd H. Kasper, Kathy A. Green, Joseph D. Schwartzman
Dartmouth Scholarship
Toxoplasma gondii remains a serious cause of morbidity and mortality in individuals that are immunosuppressed, patients with AIDS in particular. The cellular immune response, especially by gamma interferon (IFN-γ)-producing CD8+ T cells, is an essential component of protective immunity against the parasite. In the present study the role of CD8+ T cells during the reactivation of Toxoplasma infection in an immunocompromised murine model was evaluated. Chronically infected mice were challenged with LP-BM5 virus, and the kinetics of CD8+ T-cell function was studied. At 10 weeks after viral infection, mice showed obvious signs of systemic illness and began …
Resonant Enhancement Of Relativistic Electron Fluxes During Geomagnetically Active Periods, I Roth, M Temerin, M K. Hudson
Resonant Enhancement Of Relativistic Electron Fluxes During Geomagnetically Active Periods, I Roth, M Temerin, M K. Hudson
Dartmouth Scholarship
The strong increase in the ̄ux of relativistic electrons during the recovery phase of magnetic storms and during other active periods is investigated with the help of Hamiltonian formalism and simulations of test electrons which interact with whistler waves. The intensity of the whistler waves is enhanced signi®cantly due to injection of 10±100 keV electrons during the substorm. Electrons which drift in the gradient and curvature of the magnetic ®eld generate the rising tones of VLF whistler chorus. The seed population of relativ- istic electrons which bounce along the inhomogeneous magnetic ®eld, interacts resonantly with the whistler waves. Whistler wave …
The Kinesin-Related Protein, Hset, Opposes The Activity Of Eg5 And Cross-Links Microtubules In The Mammalian Mitotic Spindle, Vicki Mountain, Calvin Simerly, Louisa Howard, Asako Ando, Gerald Schatten, Duane A. Compton
The Kinesin-Related Protein, Hset, Opposes The Activity Of Eg5 And Cross-Links Microtubules In The Mammalian Mitotic Spindle, Vicki Mountain, Calvin Simerly, Louisa Howard, Asako Ando, Gerald Schatten, Duane A. Compton
Dartmouth Scholarship
We have prepared antibodies specific for HSET, the human homologue of the KAR3 family of minus end-directed motors. Immuno-EM with these antibodies indicates that HSET frequently localizes between microtubules within the mammalian metaphase spindle consistent with a microtubule cross-linking function. Microinjection experiments show that HSET activity is essential for meiotic spindle organization in murine oocytes and taxol-induced aster assembly in cultured cells. However, inhibition of HSET did not affect mitotic spindle architecture or function in cultured cells, indicating that centrosomes mask the role of HSET during mitosis. We also show that (acentrosomal) microtubule asters fail to assemble in vitro without …
Dynactin Is Required For Microtubule Anchoring At Centrosomes, N J. Quintyne, S. R. Gill, D M. Eckley, C L. Crego, D A. Compton, T A. Schroer
Dynactin Is Required For Microtubule Anchoring At Centrosomes, N J. Quintyne, S. R. Gill, D M. Eckley, C L. Crego, D A. Compton, T A. Schroer
Dartmouth Scholarship
The multiprotein complex, dynactin, is an integral part of the cytoplasmic dynein motor and is required for dynein-based motility in vitro and in vivo. In living cells, perturbation of the dynein–dynactin interaction profoundly blocks mitotic spindle assembly, and inhibition or depletion of dynein or dynactin from meiotic or mitotic cell extracts prevents microtubules from focusing into spindles. In interphase cells, perturbation of the dynein–dynactin complex is correlated with an inhibition of ER-to-Golgi movement and reorganization of the Golgi apparatus and the endosome–lysosome system, but the effects on microtubule organization have not previously been defined. To explore this question, we overexpressed …
Sar By Ms For Functional Genomics (Structure-Activity Relation By Mass Spectrometry), Bruce Randall Donald, Chris Bailey-Kellogg, John J. Kelley Iii, Cliff Stein
Sar By Ms For Functional Genomics (Structure-Activity Relation By Mass Spectrometry), Bruce Randall Donald, Chris Bailey-Kellogg, John J. Kelley Iii, Cliff Stein
Computer Science Technical Reports
Large-scale functional genomics will require fast, high-throughput experimental techniques, coupled with sophisticated computer algorithms for data analysis and experiment planning. In this paper, we introduce a combined experimental-computational protocol called Structure-Activity Relation by Mass Spectrometry (SAR by MS), which can be used to elucidate the function of protein-DNA or protein-protein complexes. We present algorithms for SAR by MS and analyze their complexity. Carefully-designed Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight (MALDI TOF) and Electrospray Ionization (ESI) assays require only femtomolar samples, take only microseconds per spectrum to record, enjoy a resolution of up to one dalton in $10^6$, and (in the case of …
A Game-Theoretic Formulation Of Multi-Agent Resource Allocation, Jonathan Bredin, Rajiv T. Maheswaran, Cagri Imer, Tamer Basar, David Kotz, Daniela Rus
A Game-Theoretic Formulation Of Multi-Agent Resource Allocation, Jonathan Bredin, Rajiv T. Maheswaran, Cagri Imer, Tamer Basar, David Kotz, Daniela Rus
Computer Science Technical Reports
This paper considers resource allocation in a network with mobile agents competing for computational priority. We formulate this problem as a multi-agent game with the players being agents purchasing service from a common server. We show that there exists a computable Nash equilibrium when agents have perfect information into the future. We simulate a network of hosts and agents using our strategy to show that our resource-allocation mechanism effectively prioritizes agents according to their endowments.
Interests, Institutions, And Ideology In Securing Policy Change: The Republican Conversion To Trade Liberalization After Smoot‐Hawley, Douglas A. Irwin, Randall S. Kroszner
Interests, Institutions, And Ideology In Securing Policy Change: The Republican Conversion To Trade Liberalization After Smoot‐Hawley, Douglas A. Irwin, Randall S. Kroszner
Dartmouth Scholarship
This paper investigates how changes in both institutional incentives and economic interests are important for securing durable changes in economic policy. We study how bipartisan support developed to sustain the Reciprocal Trade Agreements Act (RTAA) of 1934, which fundamentally transformed U.S. trade policy. The durability of this change was achieved only when the Republicans, long‐time supporters of high tariffs who originally vowed to repeal the RTAA, began to support this Democratic initiative in the 1940s. We find little evidence of an ideological shift among Republicans, but rather an increased sensitivity to export interests for which the institutional structure of the …
The Globalization Of Production And The Changing Benefits Of Conquest, Stephen Brooks
The Globalization Of Production And The Changing Benefits Of Conquest, Stephen Brooks
Dartmouth Scholarship
This article examines the conditions under which conquest is likely to reap significant economic rewards. Scholars have largely focused on how the level of popular resistance within the vanquished country influences the benefits of conquest. What needs to be scrutinized in greater depth is how post–World War II economic transformations within the most advanced countries affect the benefits of conquest. This article focuses on examining one particular economic change that has been neglected for the most part in the secu- rity and peace literature: the globalization of production. The article delineates four recent changes in the structure of global production …
Immune Responses Induced By Gene Gun Or Intramuscular Injection Of Dna Vaccines That Express Immunogenic Regions Of The Serine Repeat Antigen From Plasmodium Falciparum, Alexia A. Belperron, David Feltquate, Barbara A. Fox, Toshihiro Horii, David J. Bzik
Immune Responses Induced By Gene Gun Or Intramuscular Injection Of Dna Vaccines That Express Immunogenic Regions Of The Serine Repeat Antigen From Plasmodium Falciparum, Alexia A. Belperron, David Feltquate, Barbara A. Fox, Toshihiro Horii, David J. Bzik
Dartmouth Scholarship
The liver- and blood-stage-expressed serine repeat antigen (SERA) of Plasmodium falciparum is a candidate protein for a human malaria vaccine. We compared the immune responses induced in mice immunized with SERA-expressing plasmid DNA vaccines delivered by intramuscular (i.m.) injection or delivered intradermally by Gene Gun immunization. Mice were immunized with a pcdna3 plasmid encoding the entire 47-kDa domain of SERA (amino acids 17 to 382) or the N-terminal domain (amino acids 17 to 110) of SERA. Minimal antibody responses were detected following DNA vaccination with the N-terminal domain of SERA, suggesting that the N-terminal domain alone is not highly immunogenic …
Differential Transcription Of The Tcpph Operon Confers Biotype-Specific Control Of The Vibrio Cholerae Toxr Virulence Regulon, Yvette M. Murley, Patricia A. Carroll, Karen Skorupski, Ronald K. Taylor, Stephen B. Calderwood
Differential Transcription Of The Tcpph Operon Confers Biotype-Specific Control Of The Vibrio Cholerae Toxr Virulence Regulon, Yvette M. Murley, Patricia A. Carroll, Karen Skorupski, Ronald K. Taylor, Stephen B. Calderwood
Dartmouth Scholarship
Epidemic strains of Vibrio cholerae O1 are divided into two biotypes, classical and El Tor. In both biotypes, regulation of virulence gene expression depends on a cascade in which ToxR activates expression of ToxT, and ToxT activates expression of cholera toxin and other virulence genes. In the classical biotype, maximal expression of this ToxR regulon in vitro occurs at 30 degrees C at pH 6.5 (ToxR-inducing conditions), whereas in the El Tor biotype, production of these virulence genes only occurs under very limited conditions and not in response to temperature and pH; this difference between biotypes is mediated at the …
Stellar Masses, Kinematics, And White Dwarf Composition For Three Close Da+Dme Binaries, Stephane Vennes, John R. Thorstensen, Elisha F. Polomski
Stellar Masses, Kinematics, And White Dwarf Composition For Three Close Da+Dme Binaries, Stephane Vennes, John R. Thorstensen, Elisha F. Polomski
Dartmouth Scholarship
We determine the mass functions and mass ratios for three close white dwarf plus red dwarf binaries (EUVE J0720-317, 1016-053, and 2013+400). Hubble Space Telescope Goddard High-Resolution Spectrograph spectra of the He II λ1640 and C IV λ1550 spectral lines trace the white dwarf orbital motion, and Hamilton Spectrograph echelle spectra (Lick Observatory) and lower dispersion spectra trace the red dwarf orbital motion. The data sets allow us to measure orbital periods and velocities, as well as the white dwarf gravitational redshifts. The red dwarf and white dwarf mass estimates obtained from a combination of independent mass measurements for the …
Large‐Scale Power Spectrum And Cosmological Parameters From Sfi Peculiar Velocities, Wolfram Freudling, Idit Zehavi, Luiz N. Da Costa, Avishai Dekel, Amiram Eldar, Riccardo Giovanelli, Martha P. Haynes, John J. Salzer, Gary Wegner, Saleem Zaroubi
Large‐Scale Power Spectrum And Cosmological Parameters From Sfi Peculiar Velocities, Wolfram Freudling, Idit Zehavi, Luiz N. Da Costa, Avishai Dekel, Amiram Eldar, Riccardo Giovanelli, Martha P. Haynes, John J. Salzer, Gary Wegner, Saleem Zaroubi
Dartmouth Scholarship
We estimate the power spectrum of mass density fluctuations from peculiar velocities of galaxies by applying an improved maximum likelihood technique to the new all-sky SFI catalog. Parametric models are used for the power spectrum and the errors, and the free parameters are determined by assuming Gaussian velocity fields and errors and maximizing the probability of the data given the model. It has been applied to generalized cold dark matter (CDM) models with and without COBE normalization. The method has been carefully tested using artificial SFI catalogs. The most likely distance errors are found to be similar to the original …
Measurement Of Oxygen Partial Pressure, Its Control During Hypoxia And Hyperoxia, And Its Effect Upon Light Emission In A Bioluminescent Elaterid Larva, G. S. Timmins, C. A. A. Penatti, E. J. H. Bechara, H. M. Swartz
Measurement Of Oxygen Partial Pressure, Its Control During Hypoxia And Hyperoxia, And Its Effect Upon Light Emission In A Bioluminescent Elaterid Larva, G. S. Timmins, C. A. A. Penatti, E. J. H. Bechara, H. M. Swartz
Dartmouth Scholarship
This study investigates the respiratory physiology of bioluminescent larvae of Pyrearinus termitilluminans in relation to their tolerance to hypoxia and hyperoxia and to the supply of oxygen for bioluminescence. The partial pressure of oxygen (P(O2)) was measured within the bioluminescent prothorax by in vivo electron paramagnetic resonance (EPR) oximetry following acclimation of larvae to hypoxic, normoxic and hyperoxic (normobaric) atmospheres and during periods of bioluminescence (during normoxia). The P(O2) in the prothorax during exposure to an external P(O2) of 15.2, 160 and 760 mmHg was 10.3+/-2.6, 134+/-0.9 and 725+/-73 mmHg respectively (mean +/- s.d., N=5; 1 mmHg=0.1333 kPa). Oxygen supply …
Host-Driven Population Dynamics In An Herbivorous Insect, Tiina Ylioja, Heikki Roininen, Matthew P. Ayres, Matti Rousi, Peter W. Price
Host-Driven Population Dynamics In An Herbivorous Insect, Tiina Ylioja, Heikki Roininen, Matthew P. Ayres, Matti Rousi, Peter W. Price
Dartmouth Scholarship
Understanding the nature and relative importance of endogenous (density-dependent) and exogenous (density-independent) effects on population dynamics remains a central problem in ecology. Evaluation of these forces has been constrained by the lack of long time series of population densities and largely limited to populations chosen for their unique dynamics (e.g., outbreak insects). Especially in herbivore populations, the relative contributions of bottom-up and top-down effects (resources and natural enemies, respectively) have been difficult to compare because population data have rarely been combined with resource measurements. The feeding scars of a wood-mining herbivorous insect (Phytobia betulae Kangas; Diptera: Agromyzidae) of birch …
Vacuole Acidification Is Required For Trans-Snare Pairing, Lma1 Release, And Homotypic Fusion, Christian Ungermann, William Wickner, Zuoyu Xu
Vacuole Acidification Is Required For Trans-Snare Pairing, Lma1 Release, And Homotypic Fusion, Christian Ungermann, William Wickner, Zuoyu Xu
Dartmouth Scholarship
Vacuole fusion occurs in three stages: priming, in which Sec18p mediates Sec17p release, LMA1 (low Mr activity 1) relocation, and cis-SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complex disassembly; docking, mediated by Ypt7p and trans-SNARE association; and fusion of docked vacuoles. Ca2+ and calmodulin regulate late stages of the reaction. We now show that the vacuole proton gradient, generated by the vacuolar proton ATPase, is needed for trans-SNARE complex formation during docking and hence for the subsequent LMA1 release. Though neither the vacuolar Pmc1p Ca2+-ATPase nor the Vcx1p Ca2+/H+ exchanger …
Long-Lived Localized Field Configurations In Small Lattices: Application To Oscillons, M. Gleiser, A. Sornborger
Long-Lived Localized Field Configurations In Small Lattices: Application To Oscillons, M. Gleiser, A. Sornborger
Dartmouth Scholarship
Long-lived localized field configurations such as breathers, oscillons, or more complex objects naturally arise in the context of a wide range of nonlinear models in different numbers of spatial dimensions. We present a numerical method, which we call the adiabatic damping method, designed to study such configurations in small lattices. Using three-dimensional oscillons in φ4 models as an example, we show that the method accurately (to one part in 105 or better) reproduces results obtained with static or dynamically expanding lattices, dramatically cutting down in integration time. We further present results for two-dimensional oscillons, whose lifetimes would be prohibitively …
Mobile Agents And The Future Of The Internet, David Kotz, Robert S. Gray
Mobile Agents And The Future Of The Internet, David Kotz, Robert S. Gray
Dartmouth Scholarship
Use of the Internet has exploded in recent years with the appearance of the World-Wide Web. In this paper, we show how current technological trends may lead to a system based substantially on mobile code, and in many cases, mobile agents. We discuss several technical and non-technical hurdles along the path to that eventuality. It seems likely that, within a few years, nearly all major Internet sites will be capable of hosting and willing to host some form of mobile code or mobile agents.
Succinate Dehydrogenase (Sdh) From Bradyrhizobium Japonicum Is Closely Related To Mitochondrial Sdh, David J. Westenberg, Mary Lou Guerinot
Succinate Dehydrogenase (Sdh) From Bradyrhizobium Japonicum Is Closely Related To Mitochondrial Sdh, David J. Westenberg, Mary Lou Guerinot
Dartmouth Scholarship
The sdhCDAB operon, encoding succinate dehydrogenase, was cloned from the soybean symbiont Bradyrhizobium japonicum. Sdh from B. japonicum is phylogenetically related to Sdh from mitochondria. This is the first example of a mitochondrion-like Sdh functionally expressed in Escherichia coli.
A First Principles Warm Inflation Model That Solves The Cosmological Horizon And Flatness Problems, Arjun Berera, Marcelo Gleiser, Rudnei O. Ramos
A First Principles Warm Inflation Model That Solves The Cosmological Horizon And Flatness Problems, Arjun Berera, Marcelo Gleiser, Rudnei O. Ramos
Dartmouth Scholarship
A quantum field theory warm inflation model is presented that solves the horizon and flatness problems. The model obtains, from the elementary dynamics of particle physics, cosmological scale factor trajectories that begin in a radiation dominated regime, enter an inflationary regime, and then smoothly exit back into a radiation dominated regime, with non-negligible radiation throughout the evolution.
An Ultraviolet Fe Ii Image Of Sn 1885 In M31, Andrew Js Hamilton, Robert A. Fesen
An Ultraviolet Fe Ii Image Of Sn 1885 In M31, Andrew Js Hamilton, Robert A. Fesen
Dartmouth Scholarship
Ultraviolet imaging of the remnant of Supernova 1885 in M31 with the Hubble Space Telescope using the F255W filter on the WFPC2 reveals a dark spot of Fe II absorption at the remnant’s known position′′ ′′in the bulge of M31. The diameter of the absorbing spot is 0. 55 ± 0. 15, slightly smaller than, but′′ ′′consistent with, the 0. 70 ± 0. 05 diameter measured in the higher quality WFPC2 Ca II absorption image previously reported by us. The measured ratio of flux inside to outside SNR 1885 in the Fe IIimage is 0.24 ± 0.17, consistent with the …
A Vibrio Cholerae Lysr Homolog, Aphb, Cooperates With Apha At The Tcpph Promoter To Activate Expression Of The Toxr Virulence Cascade, Gabriela Kovacikova, Karen Skorupski
A Vibrio Cholerae Lysr Homolog, Aphb, Cooperates With Apha At The Tcpph Promoter To Activate Expression Of The Toxr Virulence Cascade, Gabriela Kovacikova, Karen Skorupski
Dartmouth Scholarship
We describe here a new member of the LysR family of transcriptional regulators, AphB, which is required for activation of the Vibrio cholerae ToxR virulence cascade. AphB activates the transcription of the tcpPH operon in response to environmental stimuli, and this process requires cooperation with a second protein, AphA. The expression of neither aphA or aphB is strongly regulated by environmental stimuli, raising the possibility that the activities of the proteins themselves may be influenced under various conditions. Strains of the El Tor biotype of V. cholerae typically exhibit lower expression of ToxR-regulated virulence genes in vitro than classical strains …
Core-Binding Factor Influences The Disease Specificity Of Moloney Murine Leukemia Virus, Amy F. Lewis, Terryl Stacy, William R. Green, Lekidelu Taddesse-Heath, Janet W. Hartley, Nancy A. Speck
Core-Binding Factor Influences The Disease Specificity Of Moloney Murine Leukemia Virus, Amy F. Lewis, Terryl Stacy, William R. Green, Lekidelu Taddesse-Heath, Janet W. Hartley, Nancy A. Speck
Dartmouth Scholarship
The core site in the Moloney murine leukemia virus (Moloney MLV) enhancer was previously shown to be an important determinant of the T-cell disease specificity of the virus. Mutation of the core site resulted in a significant shift in disease specificity of the Moloney virus from T-cell leukemia to erythroleukemia. We and others have since determined that a protein that binds the core site, one of the core-binding factors (CBF) is highly expressed in thymus and is essential for hematopoiesis. Here we test the hypothesis that CBF plays a critical role in mediating pathogenesis of Moloney MLV in vivo. We …