Digital Commons Network^™

Detection Of Human, Porcine And Canine Picornaviruses In Municipal Sewage Sludge Using Pan-Enterovirus Amplicon-Based Long-Read Illumina Sequencing, Temitope O. C. Faleye, Erin M. Driver, Devin A. Bowes, Rochelle H. Holm, Daymond Talley, Ray Yeager, Aruni Bhatnagar, Ted Smith, Arvind Varsani, Rolf U. Halden, Matthew Scotch

Faculty Scholarship

We describe the successful detection of human, porcine and canine picornaviruses (CanPV) in sewage sludge (at each stage of treatment) from Louisville, Kentucky, USA, using Pan-enterovirus amplicon-based long-read Illumina sequencing. Based on publicly available sequence data in GenBank, this is the first detection of CanPV in the USA and the first detection globally using wastewater-based epidemiology. Our findings also suggest there might be clusters of endemic porcine enterovirus (which have been shown capable of causing systemic infection in porcine) circulation in the USA that have not been sampled for around two decades. Our findings highlight the value of WBE coupled …

Probiotic Bc30 Improves Amino Acid Absorption From Plant Protein Concentrate In Older Women, Kylie E. Walden, Anthony M. Hagele, Logan S. Orr, Kristen N. Gross, Joesi M. Krieger, Ralf Jäger, Chad M. Kerksick

Faculty Scholarship

Weizmannia coagulans GBI-30, 6086 (BC30) has previously been shown to increase protein digestion in an in vitro model of the stomach and small intestine and amino acid appearance in healthy men and women after ingestion of milk protein concentrate. The impact of ingesting BC30 with other protein sources or in other demographics is largely unknown. The purpose of this study was to examine the impact of adding BC30 to a 20-g dose of a blend of rice and pea protein on postprandial changes in blood amino acids concentrations in healthy, older women. Healthy, older females (n = 30, 58.5 …

The Mediating Effect Of Caregiving Relationship Quality On The Association Between Caregiving Stressors And Mental Health Problems Among Older Spousal Caregivers, Fei Wang, Elliane Irani

Faculty Scholarship

Providing care to a spouse can be especially challenging for older adults given their compounding stressors resulting from aging and caregiving. This cross-sectional study examines the relationships between caregiving stressors and caregiver mental health problems and the potential mediator (i.e., caregiving relationship quality) of these associations. A total of 431 Americans (≥65 years) were selected from the National Study of Caregiving. Path analysis shows that care assistance was positively associated with caregiver mental health problems, and this association was mediated by negative relationship quality (Indirect effect =.14, p =.016). Moreover, role overload was positively associated with caregiver mental health problems, …

Pum1 Mediates The Posttranscriptional Regulation Of Human Fetal Hemoglobin, Yuncheng Man, Amma Owusu-Ansah, Omar Y. Mian, Umut A. Gurkan, Anton A. Komar, Merlin Nithya Gnanapragasam

Faculty Scholarship

The fetal-to-adult hemoglobin switching at about the time of birth involves a shift in expression from γ-globin to β-globin in erythroid cells. Effective re-expression of fetal γ-globin can ameliorate sickle cell anemia and β-thalassemia. Despite the physiological and clinical relevance of this switch, its posttranscriptional regulation is poorly understood. Here, we identify Pumilo 1 (PUM1), an RNA-binding protein with no previously reported functions in erythropoiesis, as a direct posttranscriptional regulator of β-globin switching. PUM1, whose expression is regulated by the erythroid master transcription factor erythroid Krüppel-like factor (EKLF/KLF1), peaks during erythroid differentiation, binds γ-globin messenger RNA (mRNA), and reduces γ-globin …

Socioeconomic Deprivation And Cardiometabolic Risk Factors In Individuals With Type 1 Diabetes: T1d Exchange Clinic Registry, Stephanie Griggs, Sadeer Al-Kindi, Heather Hardin, Elliane Irani, Sanjay Rajagopalan, Ronald L. Hickman Jr.

Faculty Scholarship

Aims: Social determinants of health (SDOH) influence cardiovascular health in the general population; however, the degree to which this occurs in individuals with type 1 diabetes (T1D) is not well understood. We evaluated associations among socioeconomic deprivation and cardiometabolic risk factors (hemoglobin A1c, low-density lipoprotein, blood pressure, body mass index, physical activity) in individuals with T1D from the T1D Clinic Exchange Registry. Methods: We evaluated the association between the social deprivation index (SDI) and cardiometabolic risk factors using multivariable and logistic regression among 18,754 participants ages 13 – 90 years (mean 29.2 ± 17) in the T1D Exchange clinic registry …

Moral Status And The Oversight Of Research Involving Chimeric Animals, Patricia Marshall, Kaitlynn P. Craig

Faculty Scholarship

The use of nonhuman animals in research has long been a source of bioethical and scientific debate. We consider the oversight and use of nonhuman animals in chimeric research. We conducted interviews with twelve members of embryonic stem cell research oversight committees, nine members of institutional animal care and use committees, and fourteen scientists involved in human–nonhuman-animal chimeric research in different areas of the United States. Interviews addressed animal welfare and conceptual issues associated with moral status and humanization of nonhuman animals that contain human cells. Our findings suggest that concepts of enhanced moral status and consciousness are not very …

Galaxy And Mass Assembly (Gama): The Dependence Of Star Formation On Surface Brightness In Low-Redshift Galaxies, S Phillipps, S Bellstedt, M N. Bremer, R De Propris, P A. James, S Casura, J Liske, B W. Holwerda

Faculty Scholarship

The star-formation rate in galaxies is well known to correlate with stellar mass (the ‘star-forming main sequence’). Here, we extend this further to explore any additional dependence on galaxy surface brightness, a proxy for stellar mass surface density. We use a large sample of low-redshift (z ≤ 0.08) galaxies from the Galaxy And Mass Assembly survey which have both spectral energy distribution (SED) derived star-formation rates and photometric bulge-disc decompositions, the latter providing measures of disc surface brightness and disc masses. Using two samples, one of galaxies fitted by a single component with Sérsic index below 2 and one …

Fostering Durable Skills Development: Leveraging Student Worker Programs, James Hutson, Elizabeth Macdonald, Lisa Young, Susan Edele, Chris Smentkowski

Faculty Scholarship

The past half century has witnessed exponential increases in the cost of a college degree paralleled by an increase of undergraduate students who are employed during matriculation. While the impact on learning outcomes due to off-campus employment continues to receive much attention, there has been little attention paid to students employed on-campus through student worker programs and their benefits, especially in developing durable skills and improving retention. Research has demonstrated that engagement in oncampus activities improves retention and persistence in students. Additionally, employers desire power or durable skills (formerly known as “soft skills”), and that student worker programs are suited …

Would You Make It To The Future? Teaching Race In An Assisted Reproductive Technologies And The Law Classroom, Sonia Gipson Rankin

Faculty Scholarship

Would you make it to the future? For the last five years, I have started my Assisted Reproductive Technology (ART) lecture in Family Law with this question. Students take the query seriously. They ponder their lived experiences such as home training, medical history, education, financial well-being, personality traits, work ethic, and social graces when determining if they would be the “model DNA” someone might select in a future society. The good-natured jokes about being nearsighted, having a pitiful jump shot, and wearing orthodontic headgear turn reflective when someone raises the question: would someone in the future select my race? In …

Multispectral Imaging For Microchip Electrophoresis Enables Point-Of-Care Newborn Hemoglobin Variant Screening, Ran An, Yuning Huang, Anne Rocheleau, Qiaochu Zhang, Yuncheng Man, Zoe Sekyonda, Amma Owusu-Ansah, Umut A. Gurkan

Faculty Scholarship

Hemoglobin (Hb) disorders affect nearly 7% of the world's population. Globally, around 400,000 babies are born annually with sickle cell disease (SCD), primarily in sub-Saharan Africa where morbidity and mortality rates are high. Screening, early diagnosis, and monitoring are not widely accessible due to technical challenges and cost. We hypothesized that multispectral imaging will allow sensitive hemoglobin variant identification in existing affordable paper-based Hb electrophoresis. To test this hypothesis, we developed the first integrated point-of-care multispectral Hb variant test: Gazelle-Multispectral. Here, we evaluated the accuracy of Gazelle-Multispectral for Hb variant newborn screening in 265 newborns with known hemoglobin variants including …

Estimating Sewage Flow Rate In Jefferson County, Kentucky, Using Machine Learning For Wastewater-Based Epidemiology Applications, Dhiraj Kanneganti, Lauren E. Reinersman, Rochelle H. Holm, Ted Smith

Faculty Scholarship

Direct measurement of the flow rate in sanitary sewer lines is not always feasible and is an important parameter for the normalization of data used in wastewater-based epidemiology applications. Machine learning to estimate past wastewater influent flow rates supporting public health applications has not been studied. The aim of this study was to assess wastewater treatment plant influent flow rates when compared with weather data and to retrospectively estimate flow rates in Louisville, Kentucky (USA), based on other data types using machine learning. A random forest model was trained using a range of variables, such as feces-related indicators, weather data …

"The Lady Took Me To The End Of The World!": The Life Of Mrs. N.A. Courtright., Marcus Walker

Faculty Scholarship

Nellie Almee Courtright was the first female to earn a law degree from the University of Louisville School of Law, but she had an accomplished career before -- and even after -- she stepped foot into a law classroom. This is the account of a woman who made her own way in the world, and made life better for hundreds in doing so.

Artificial Intelligence And The Disruption Of Higher Education: Strategies For Integrations Across Disciplines, James Hutson, Theresa Jeevanjee, Vanessa Vander Graaf, Jason Lively, Joe Weber, Graham Weir, Kathryn Arnone, Geremy Carnes, Kathi Vosevich, Daniel Plate, Michael Leary, Susan Edele

Faculty Scholarship

Artificial intelligence (AI) and its impact on society have received a great deal of attention in the past five years since the first Stanford AI100 report. AI already globally impacts individuals in critical and personal ways, and many industries will continue to experience disruptions as the full algorithmic effects are understood. Higher education is one of the industries that will be greatly impacted; consequently, many institutions have begun accelerating its adoption across disciplines to address the fast-approaching market shift. Recent advances with the technology are especially promising for its potential to create and scale personalized learning for students, to optimize …

Designing For Justice: Pandemic Lessons For Criminal Courts, Cynthia Alkon

Faculty Scholarship

March 2020 brought an unprecedented crisis to the United States: COVID-19. In a two-week period, criminal courts across the country closed. But, that is where the uniformity ended. Criminal courts did not have a clear process to decide how to conduct necessary business. As a result, criminal courts across the country took different approaches to deciding how to continue necessary operations and in doing so many did not consider the impact on justice of the operational changes that were made to manage the COVID-19 crisis. One key problem was that many courts did not use inclusive processes and include all …

The Impact Of Methodology On The Reproducibility And Rigor Of Dna Methylation Data, Detlev Boison, Susan A. Masino, Farah D. Lubin, Kai Guo, Theresa Lusardi, Richard Sanchez, David N. Ruskin, Joyce Ohm, Jonathan D. Geiger, Junguk Hur

Faculty Scholarship

Epigenetic modifications are crucial for normal development and implicated in disease pathogenesis. While epigenetics continues to be a burgeoning research area in neuroscience, unaddressed issues related to data reproducibility across laboratories remain. Separating meaningful experimental changes from background variability is a challenge in epigenomic studies. Here we show that seemingly minor experimental variations, even under normal baseline conditions, can have a significant impact on epigenome outcome measures and data interpretation. We examined genome-wide DNA methylation and gene expression profiles of hippocampal tissues from wild-type rats housed in three independent laboratories using nearly identical conditions. Reduced-representation bisulfite sequencing and RNA-seq respectively …

Optimizing Disaster Preparedness Planning For Minority Older Adults: One Size Does Not Fit All, Omolola E. Adepoju, Luz E. Herrera, Minji Chae, Daikwon Han

Faculty Scholarship

By 2050, one in five Americans will be 65 years and older. The growing proportion of older adults in the U.S. population has implications for many aspects of health including disaster preparedness. This study assessed correlates of disaster preparedness among community-dwelling minority older adults and explored unique differences for African American and Hispanic older adults. An electronic survey was disseminated to older minority adults 55+, between November 2020 and January 2021 (n = 522). An empirical framework was used to contextualize 12 disaster-related activities into survival and planning actions. Multivariate logistic regression models were stratified by race/ethnicity to examine the …

Novel Screening Tool And Considerations For Music Therapists Serving Autistic Individuals Via Telehealth: Qualitative Results From A Survey Of Clinicians’ Experiences, Nicole M. Richard Williams

Faculty Scholarship

During the COVID-19 pandemic, music therapists transitioned services from in-person to telehealth due to health and safety concerns. Though online delivery of music therapy services for autistic individuals occurred prior to 2020, the number of North American music therapists using telehealth with autistic clients rose substantially during the pandemic. The current paper’s objective was to delineate music therapists’ perceptions regarding factors that helped or hindered autistic persons’ engagement in online music therapy sessions. One-hundred ninety-two participants completed the survey. Qualitative content analysis of an open-ended question identified seven overarching themes regarding benefits and challenges of telehealth music therapy for autistic …

The Private Law Of Stablecoins, Kara J. Bruce, Christopher K. Odinet, Andrea Tosato

Faculty Scholarship

Stablecoins are one of the cornerstones of the crypto world. They’ve attracted significant attention over the past few years, ranging from Wall Street to kitchen table investors, and even the White House. As a less volatile alternative to crypto-assets like bitcoin, stablecoins have the potential to change the way we make payments, unlock the groundwork needed for more blockchain-based applications, and even reorient the economy toward private money. But how stable are these stablecoins, really? Can they be relied upon in the way their many proponents claim? And how much of the popular beliefs about stablecoins match their realities? That’s …

The Role Of Virtual Reality In Art Therapy To Mitigate Autism Spectrum Disorder (Asd) Symptoms, James Hutson

Faculty Scholarship

Traditional art therapy sessions are designed for face-to-face engagement with patients as with other mental health services such as counseling. However, the pandemic of 2019 severely limited access to these services and, initially, led to widespread cancelation and postponement until the nature of COVID-19 could be better understood. When treatment resumed, video conferencing was the preferred method of holding therapy sessions one-on-one to diagnose patients and provide services. Similar approaches were taken in art therapy with less efficacy given the hands-on nature of the required activities. With the rise of virtual reality (VR) as a more widely accessible technology since …

Commentary On Reynolds V. Mcnichols, Aziza Ahmed

Faculty Scholarship

The 1973 case Reynolds v McNichols concerns a woman who was repeatedly arrested on suspicion of and for “prostitution.” During these arrests, Roxanne Reynolds, the defendant, was subject to forced examination and treatment. The arrests and examinations were authorized by Section 735 of the Revised Municipal Code of the City and County of Denver, which directed the Department of Health and Hospitals “to use every available means to ascertain the existence of and investigate all suspected cases of communicable venereal disease, and to determine the sources of such infections.” Reynolds argued that the ordinance was unconstitutional because it was irrational, …

Contracts Scholarship Beyond Materialisierung, Daniela Caruso

Faculty Scholarship

This comment aims to show how Klaus Eller's paper on ‘The Political Economy of Tenancy Contract Law’¹ raises the stakes of private law scholarship and contributes to the larger project of remodeling legal institutions in a progressive direction. The comment starts by contextualising the rapid spread of the Law and Political Economy (LPE) movement; illustrates through examples the generative impact of LPE on contemporary contracts scholarship; and highlights two strands of Eller’s original contribution to such literature: a welcome reflection on the value and limits of Materialisierung, and a radical widening of the private law inquiry to include …

Galaxy And Mass Assembly (Gama): Extended Intragroup Light In A Group At Z = 0.2 From Deep Hyper Suprime-Cam Images, Cristina Martínez-Lombilla, Sarah Brough, Mireia Montes, Roberto Baena-Gallé, Mohammad Akhlaghi, Raúl Infante-Sainz, Simon P. Driver, Benne W. Holwerda, Kevin A. Pimbblet, Aaron S G Robotham

Faculty Scholarship

We present a pilot study to assess the potential of Hyper Suprime-Cam Public Data Release 2 (HSC-PDR2) images for the analysis of extended faint structures within groups of galaxies. We examine the intragroup light (IGL) of the group 400138 (M_dyn = 1.3 ± 0.5 × 1013 M_⊙, z ∼ 0.2) from the Galaxy And Mass Assembly (GAMA) survey using Hyper Suprime-Cam Subaru Strategic Program Public Data Release 2 (HSC-SSP PDR2) images in g, r, and i bands. We present the most extended IGL measurement to date, reaching down to μglim=30.76" role="presentation" style="box-sizing: border-box; margin: 0px; …

Wallaby Pilot Survey: Hi Gas Kinematics Of Galaxy Pairs In Cluster Environment, Shin-Jeong Kim, Minsu Kim, Hye-Jin Park, Shinna Kim, Bigiel Frank, Helga Denes, Bi-Qing For, Peter Kamphuis, Se-Heon Oh, Benne Holwerda, Kristen Mcquinn, Juan Madrid, Ahmed Elagali, Gerhardt R. Meurer, Kristine Spekkens, Lister Staveley-Smith, Barbara Catinella, Nathan Deg, Karen Lee-Waddell, Jing Wang, Lourdes Verdes-Montenegro, Ivy Wong, Jonghwan Rhee

Faculty Scholarship

We examine the H I gas kinematics of galaxy pairs in two clusters and a group using Australian Square Kilometre Array Pathfinder (ASKAP) WALLABY pilot survey observations. We compare the H I properties of galaxy pair candidates in the Hydra I and Norma clusters, and the NGC 4636 group, with those of non-paired control galaxies selected in the same fields. We perform H I profile decomposition of the sample galaxies using a tool, BAYGAUD which allows us to de-blend a line-of-sight velocity profile with an optimal number of Gaussian components. We construct H I super-profiles of the sample galaxies via …

Social Determinants Of Health And Child Maltreatment Prevention: The Family Success Network Pilot, Nancy Rolock, David Crampton

Faculty Scholarship

Child maltreatment is a highly prevalent public health concern that contributes to morbidity and mortality in childhood and short- and long-term health consequences that persist into adulthood. Past research suggests that social determinants of health such as socioeconomic status and intergenerational trauma are highly correlated with child maltreatment. With support from the U.S. Children’s Bureau, the Ohio Children’s Trust Fund is currently piloting the Family Success Network, a primary child maltreatment prevention strategy in Northeast Ohio that seeks to address these social determinants through pillars of service that include family coaching, financial assistance, financial education, parenting education, and basic life …

Effects Of Creatine Monohydrate Timing On Resistance Training Adaptations And Body Composition After 8 Weeks In Male And Female Collegiate Athletes, Nicholas E. Dinan, Anthony Hagele, Andrew R. Jagim, Michael G. Miller, Chad M. Kerksick

Faculty Scholarship

Background: Limited research is available on the potential impact of creatine monohydrate administration before or after workouts among athletes. This study aimed to investigate the effects of pre- vs. post-exercise creatine monohydrate supplementation on resistance training adaptations and body composition.

Methods: In a randomized, double-blind, placebo-controlled, parallel design, 34 healthy resistance-trained male and female athletes were randomly assigned and matched according to fat free mass to consume a placebo, or 5-g dose of creatine monohydrate within 1 h before training, or within 1 h after training for 8 weeks, while completing a weekly resistance training program. Participants co-ingested 25-gram doses …

Catch Bonds In Sickle Cell Disease: Shear-Enhanced Adhesion Of Red Blood Cells To Laminin, Utku Goreke, Shamreen Iram, Gundeep Singh, Sergio Domínguez-Medina, Yuncheng Man, Allison Bode, Ran An, Jane A. Little, Christopher L. Wirth, Michael Hinczewski, Umut A. Gurkan

Faculty Scholarship

Could the phenomenon of catch bonding—force-strengthened cellular adhesion—play a role in sickle cell disease, where abnormal red blood cell (RBC) adhesion obstructs blood flow? Here we investigate the dynamics of sickle RBCs adhering to a surface functionalized with the protein laminin (a component of the extracellular matrix around blood vessels) under physiologically relevant micro-scale flow. First, using total internal reflectance microscopy we characterize the spatial fluctuations of the RBC membrane above the laminin surface before detachment. The complex dynamics we observe suggest the possibility of catch bonding, where the mean detachment time of the cell from the surface initially increases …

Devils: Cosmic Evolution Of Sed-Derived Metallicities And Their Connection To Star Formation Histories, Jessica E. Thorne, Aaron S G Robotham, Sabine Bellstedt, Luke J M Davies, Robin H W Cook, Luca Cortese, Benne Holwerda, Steven Phillipps, Malgorzata Siudek

Faculty Scholarship

Gas-phase metallicities of galaxies are typically measured through auroral or nebular emission lines, but metallicity also leaves an imprint on the overall spectral energy distribution (SED) of a galaxy and can be estimated through SED fitting. We use the PROSPECT SED fitting code with a flexible parametric star formation history and an evolving metallicity history to self-consistently measure metallicities, stellar mass, and other galaxy properties for 90 000 galaxies from the Deep Extragalactic VIsible Legacy Survey (DEVILS) and Galaxy and Mass Assembly (GAMA) survey. We use these to trace the evolution of the mass–metallicity relation (MZR) and show that the …

Behavior Of Concrete Reinforced With Date Palm Fibers, Elias Ali

Faculty Scholarship

In recent decades, researchers have begun to investigate innovative sustainable construction materials for the development of greener and more environmentally friendly infrastructures. The main purpose of this article is to investigate the possibility of employing date palm tree waste as a natural fiber alternative for conventional steel and polypropylene fibers (PPFs) in concrete. Date palm fibers are a common agricultural waste in Middle Eastern nations, particularly Saudi Arabia. As a result, this research examined the engineering properties of high‐strength concrete using date palm fibers, as well as the performance of traditional steel and PPF concrete. The concrete samples were made …

Wallaby Pre-Pilot And Pilot Survey: The Tully Fisher Relation In Eridanus, Hydra, Norma And Ngc4636 Fields, Helene Courtois, Alexandra Dupuy, Kristen Mcquinn, Lister Staveley-Smith, Danail Obreschkow, Edward Taylor, Benne Holwerda, Cullan Howlett, Frank Bigiel, Daniel Pomarede, Bi-Qing For, Tao Hong, Dane Kleiner, Thomas Jarrett, Ivy Wong, Jonghwan Rhee, Albert Bosma, Khaled Said, Tamara Davis, Igor Karachentsev, Matthew Colless, Renee Kraan-Korteweg, Nathan Deg, Kristine Spekkens, Karen Lee-Waddell, Jing Wang, Gerhardt R. Meurer

Faculty Scholarship

The WALLABY pilot survey has been conducted using the Australian SKA Pathfinder (ASKAP). The integrated 21-cm HI line spectra are formed in a very different manner compared to usual single-dish spectra Tully-Fisher measurements. It is thus extremely important to ensure that slight differences (e.g. biases due to missing flux) are quantified and understood in order to maximise the use of the large amount of data becoming available soon. This article is based on four fields for which the data are scientifically interesting by themselves. The pilot data discussed here consist of 614 galaxy spectra at a rest wavelength of 21cm. …

Grafting Of Short Elastin-Like Peptides Using An Electric Field, Nuttanit Pramounmat, Sogol Asaei, Jacob D. Hostert, Horst A. Von Recum, Julie N. Renner

Faculty Scholarship

Surface-grafted elastin has found a wide range of uses such as sensing, tissue engineering and capture/release applications because of its ability to undergo stimuli-responsive phase transition. While various methods exist to control surface grafting in general, it is still difficult to control orientation as attachment occurs. This study investigates using an electric field as a new approach to control the surface-grafting of short elastin-like polypeptide (ELP). Characterization of ELP grafting to gold via quartz crystal microbalance with dissipation, atomic force microscopy and temperature ramping experiments revealed that the charge/hydrophobicity of the peptides, rearrangement kinetics and an applied electric field impacted …