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Articles 1 - 11 of 11

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Genetically Encoded Live-Cell Sensor For Tyrosinated Microtubules, Shubham Kesarwani, Prakash Lama, Anchal Chandra, P. Purushotam Reddy Oct 2020

Genetically Encoded Live-Cell Sensor For Tyrosinated Microtubules, Shubham Kesarwani, Prakash Lama, Anchal Chandra, P. Purushotam Reddy

Open Access archive

Microtubule cytoskeleton exists in various biochemical forms in different cells due to tubulin posttranslational modifications (PTMs). Tubulin PTMs are known to affect microtubule stability, dynamics, and interaction with MAPs and motors in a specific manner, widely known as tubulin code hypothesis. At present, there exists no tool that can specifically mark tubulin PTMs in living cells, thus severely limiting our understanding of their dynamics and cellular functions. Using a yeast display library, we identified a binder against terminal tyrosine of α-tubulin, a unique PTM site. Extensive characterization validates the robustness and nonperturbing nature of our binder as tyrosination sensor, a …


Docking Andin Vitromolecular Biology Studies Ofp-Anisidine-Appended 1-Hydroxy-2-Acetonapthanone Schiff Base Lanthanum(Iii) Complexes, V. Sathiyanarayanan, P. Varun Prasath, P. Chandra Sekhar, K. Ravichandran Apr 2020

Docking Andin Vitromolecular Biology Studies Ofp-Anisidine-Appended 1-Hydroxy-2-Acetonapthanone Schiff Base Lanthanum(Iii) Complexes, V. Sathiyanarayanan, P. Varun Prasath, P. Chandra Sekhar, K. Ravichandran

Open Access archive

A new series of lanthanum(iii) complexes was synthesized using ap-anisidine-appended 1-hydroxy-2-acetonapthanone (3) Schiff base and characterizedviaspectroscopic methods. The ligand was synthesizedviasonication and the crystalline product was characterized using X-ray crystallography. The genotoxicity of the compound was assessed primarily by the bacterial reverse mutation (Ames) test and thein vitromammalian chromosome aberration test; in both cases, the samarium complex5was found to be non-mutagenic. The anti-tumor activity of complexes45, and6was assayed against HeLa tumor cells and screened using the MTT assay. The IC50value of complex5was found to be 34 ± 1.2 μg mL−1and this compound exhibited superior activity towards the cells compared to4and6. …


Omega-3 Fatty Acids, Kristina Krupa, Kristina Fritz, Mayur S. Parmar Jan 2020

Omega-3 Fatty Acids, Kristina Krupa, Kristina Fritz, Mayur S. Parmar

HPD Articles

No abstract provided.


Cortical Travelling Waves: Mechanisms And Computational Principles, Lyle Muller, Frédéric Chavane, John Reynolds, Terrence J. Sejnowski Apr 2018

Cortical Travelling Waves: Mechanisms And Computational Principles, Lyle Muller, Frédéric Chavane, John Reynolds, Terrence J. Sejnowski

Brain and Mind Institute Researchers' Publications

Multichannel recording technologies have revealed travelling waves of neural activity in multiple sensory, motor and cognitive systems. These waves can be spontaneously generated by recurrent circuits or evoked by external stimuli. They travel along brain networks at multiple scales, transiently modulating spiking and excitability as they pass. Here, we review recent experimental findings that have found evidence for travelling waves at single-area (mesoscopic) and whole-brain (macroscopic) scales. We place these findings in the context of the current theoretical understanding of wave generation and propagation in recurrent networks. During the large low-frequency rhythms of sleep or the relatively desynchronized state of …


Impact Of Introduced Parasitoid Cotesia Vestalis (Hymenoptera: Braconidae) On Plutella Xylostella (Lepidoptera: Plutellidae) And Its Parasitoid Guild On Kale In Semi-Arid Areas In Kenya, Ruth Kahuthia-Gathu, Brigitte Nyambo, Sevgan Subramanian Sep 2017

Impact Of Introduced Parasitoid Cotesia Vestalis (Hymenoptera: Braconidae) On Plutella Xylostella (Lepidoptera: Plutellidae) And Its Parasitoid Guild On Kale In Semi-Arid Areas In Kenya, Ruth Kahuthia-Gathu, Brigitte Nyambo, Sevgan Subramanian

All Peer-Reviewed Publications

Cotesia vestalis was introduced into Kenya from South Africa in March 2003 for biological control of Plutella xylostella with initial release in 2004. To confirm establishment, the rate of spread beyond the release sites and parasitism rates by C. vestalis were assessed between October 2011 and April 2012 in eastern and southeastern Kenya (Kitui, Mwingi/Yatta and Loitokitok areas). Monthly observations on kale infestation by diamondback moth, damage scores, parasitism and parasitoid guild were undertaken on 20 randomly selected plants in farmer-managed kale farms. Sampled larval and pupal stages were placed in containers and taken to the laboratory for parasitoid emergence. …


An Indirect Defence Trait Mediated Through Egg-Induced Maize Volatiles From Neighbouring Plants, Daniel M. Mutyambai, Toby J.A. Bruce, Johnnie Van Den Berg, Charles A.O. Midega, John A. Pickett, Zeyaur R. Khan Jul 2016

An Indirect Defence Trait Mediated Through Egg-Induced Maize Volatiles From Neighbouring Plants, Daniel M. Mutyambai, Toby J.A. Bruce, Johnnie Van Den Berg, Charles A.O. Midega, John A. Pickett, Zeyaur R. Khan

All Peer-Reviewed Publications

Attack of plants by herbivorous arthropods may result in considerable changes to the plant's chemical phenotype with respect to emission of herbivore-induced plant volatiles (HIPVs). These HIPVs have been shown to act as repellents to the attacking insects as well as attractants for the insects antagonistic to these herbivores. Plants can also respond to HIPV signals from other plants that warn them of impending attack. Recent investigations have shown that certain maize varieties are able to emit volatiles following stemborer egg deposition. These volatiles attract the herbivore's parasitoids and directly deter further oviposition. However, it was not known whether these …


Effects Of Vector Migration On Sylvatic Trypanosoma Cruzi Transmission, Britnee A. Crawford Jan 2012

Effects Of Vector Migration On Sylvatic Trypanosoma Cruzi Transmission, Britnee A. Crawford

Mathematics Dissertations - Archive

Vector-borne diseases have had a major impact on global health concerns since their discovery in the 1800's. A vector-borne disease is one transmitted to human or animal host via an invertebrate vector (usually an insect). Chagas' disease, caused by the parasite Trypanosoma cruzi, is transmitted via insect vectors from the Triatoma family. Although human infection with Chagas' is of importance, the disease is maintained in sylvatic (in the wild) transmission cycles. This study examines the effects of vector migration on the spread of T. cruzi in certain sylvatic cycles in the southeastern U.S. and Mexico from several angles. First, a …


Genome 10k: A Proposal To Obtain Whole-Genome Sequence For 10000 Vertebrate Species, David Haussler, Stephen J. O'Brien, Oliver A. Ryder, F. Keith Barker, Michele Clamp, Andrew J. Crawford, Robert Hanner, Olivier Hanotte, Warren E. Johnson, Jimmy A. Mcguire, Webb Miller, Robert W. Murphy, William J. Murphy, Frederick H. Sheldon, Barry Sinervo, Byrappa Venkatesh, Edward O. Wiley, Fred W. Allendorf, George Amato, C. Scott Baker, Aaron Bauer, Albano Beja-Pereira, Eldredge Bermingham, Giacomo Bernardi, Cibele R. Bonvicino, Sydney Brenner, Terry Burke, Joel Cracraft, Mark Diekhans Nov 2009

Genome 10k: A Proposal To Obtain Whole-Genome Sequence For 10000 Vertebrate Species, David Haussler, Stephen J. O'Brien, Oliver A. Ryder, F. Keith Barker, Michele Clamp, Andrew J. Crawford, Robert Hanner, Olivier Hanotte, Warren E. Johnson, Jimmy A. Mcguire, Webb Miller, Robert W. Murphy, William J. Murphy, Frederick H. Sheldon, Barry Sinervo, Byrappa Venkatesh, Edward O. Wiley, Fred W. Allendorf, George Amato, C. Scott Baker, Aaron Bauer, Albano Beja-Pereira, Eldredge Bermingham, Giacomo Bernardi, Cibele R. Bonvicino, Sydney Brenner, Terry Burke, Joel Cracraft, Mark Diekhans

Biology Faculty Articles

The human genome project has been recently complemented by whole-genome assessment sequence of 32 mammals and 24 nonmammalian vertebrate species suitable for comparative genomic analyses. Here we anticipate a precipitous drop in costs and increase in sequencing efficiency, with concomitant development of improved annotation technology and, therefore, propose to create a collection of tissue and DNA specimens for 10000 vertebrate species specifically designated for whole-genome sequencing in the very near future. For this purpose, we, the Genome 10K Community of Scientists (G10KCOS), will assemble and allocate a biospecimen collection of some 16203 representative vertebrate species spanning evolutionary diversity across living …


Synthetic Studies And Biological Evaluation Of Imidazole-Containing Marine Alkaloids, Lesley Ann Schmid May 2008

Synthetic Studies And Biological Evaluation Of Imidazole-Containing Marine Alkaloids, Lesley Ann Schmid

Chemistry & Biochemistry Dissertations - Archive

This dissertation discusses the synthetic methods towards and the preliminary biological studies of an unnamed, imidazole-containing alkaloid, derived from the marine species Oceanapia. This alkaloid was first discovered in 1996 by researchers at the NIH as a possible inhibitor of the key enzyme in the detoxification cycle of Mycobacterium tuberculosis. Chapter 1 provides an overview of tuberculosis, which is currently the second highest cause of death worldwide, with one third of the world's population infected. In recent years, the emergence of multi-drug resistant strains has created new interest and funding for tuberculosis research. New treatments like the alkaloid described above …


Tendencies And Perspectives Of Social Sciences In Health In Latin America, Everardo D. Nunes Apr 2008

Tendencies And Perspectives Of Social Sciences In Health In Latin America, Everardo D. Nunes

English

No abstract provided.


Search For Effective Natural Enemies Of Tetranychus Evansi (Acari: Tetranychidae) In Northwest Argentina, Imeuda P. Furtado, Silvana Toledo, Gilberto J. De Moraes, Serge Kreiter, Markus Knapp Oct 2007

Search For Effective Natural Enemies Of Tetranychus Evansi (Acari: Tetranychidae) In Northwest Argentina, Imeuda P. Furtado, Silvana Toledo, Gilberto J. De Moraes, Serge Kreiter, Markus Knapp

All Peer-Reviewed Publications

The mite Tetranychus evansi Baker & Pritchard (Tetranychidae) probably originated in South America. Because of its importance as a tomato pest in Africa, an extensive project has been conducted to detect potentially effective natural enemies in South America for the classical biological control of the pest in Africa. A search for the natural enemies of T. evansi was conducted in the Province of Tucumán, northwestern Argentina, in December 2004, and this report describes the results. One hundred predatory mites of the family Phytoseiidae referring to 11 species were collected on 11 examined species of solanaceous plants. The most abundant phytoseiid …