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Calf Diarrhea (Scours): Reproduced With A Virus From A Field Outbreak, C. A. Mebus, N. R. Underdahl, M. B. Rhodes, M. J. Twiehaus

Nebraska Agricultural Experiment Station: Historical Research Bulletins

Neonatal calf diarrhea typical of that seen in field cases was produced in colostrum-deprived calves by inoculating either feces or bacteria-free filtrates via the duodenum, orally, or by a spray (aerosol). Three calves, kept free of E. coli, developed severe diarrhea following inoculation with a bacteria-free filtrate. Intense fluorescence in the epithelium of sections of small intestine from two experimental calves was observed following staining with ftuorescein-labeled gamma globulin produced with viral antigen prepared from the feces of two experimental diarrhetic E. coli-free calves. Fluorescing cells were also present in the feces of experimental and field cases of neonatal …