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Dna Extraction And Genotyping From Burned Skeletal Remains, Kayla M. Rooney
Dna Extraction And Genotyping From Burned Skeletal Remains, Kayla M. Rooney
Student Theses
In this research, bovine leg bones were burned, both with and without meat intact, in order to determine if it was possible to extract and amplify DNA to obtain profiles. The meat was burned over an open flame and the DNA was extracted using the Qiagen DNeasy Blood & Tissue Mini Kit, quantified with the NanoDrop, and amplified using the Bovine Genotypes Panel 3.1 Kit, and separated using the 3500 Genetic Analyzer. Profiles were analyzed using the Thermo Fisher Cloud Microsatellite Analysis software. The samples burned with the meat intact produced lower quantities of DNA than the bones burned with …