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Validation Of Chimeric Viruses In Plaque Reduction Neutralization Test In Arboviral Disease Diagnostics, Jasmine Boykin
Validation Of Chimeric Viruses In Plaque Reduction Neutralization Test In Arboviral Disease Diagnostics, Jasmine Boykin
USF Tampa Graduate Theses and Dissertations
The plaque reduction neutralization test (PRNT) is a confirmatory diagnostic assay that is used to confirm a variety of diseases. The performance of PRNT requires the use of infectious wild type viruses, which increases the risk of laboratory acquired infections. For instance, eastern equine encephalitis (EEEV) is a highly virulent pathogen used in PRNT that can result in potentially fatal neurological diseases among humans and equines. Therefore, arboviral PRNT must be performed in Biosafety Level 3 (BSL-3) containment facilities and may require select agent approved scientists, like in the case of EEEV. These stringent requirements restrict the ability of public …