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Growth Substrate Induced Functional Changes Elucidated By Ftir And Raman Spectroscopy In In-Vitro Cultured Human Keratinocytes., Aidan Meade, Fiona Lyng, Peter Knief, Hugh Byrne

Articles

Non-invasive measurements of cellular function in in-vitro cultured cell lines using vibrational spectroscopy require the use of spectroscopic substrates such as quartz, ZnSe and MirrIR etc. These substrates are generally dissimilar to the original in-vivo extracellular environment of a given cell line and are often tolerated poorly by cultured cell lines resulting in morphological and functional changes in the cell. The present study demonstrates various correlations between vibrational spectroscopic analyses and biochemical analyses in the evaluation of the interaction of a normal human epithelial keratinocyte cell line (HaCaT) with MirrIR and quartz substrates coated with fibronectin, laminin and gelatin. The …