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Optimising The Ratio Of Horseradish Peroxidase And Glucose Oxidase On A Bienzyme Electrode: Comparison Of A Theoretical And Experimental Approach, Dana Mackey, Anthony Killard, Adriano Ambrosi, Malcolm Smyth

Articles

This study compares the behaviour of an electrochemical enzyme biosensor with a theoretical analysis based on a mathematical model and numerical simulation. The biosensor is based on a bi-enzyme channelling configuration, employing the enzymes glucose oxidase and horseradish peroxidase, with direct electron transfer of horseradish peroxidase at a conducting polymer electrode. This was modelled by a system of partial differential equations and boundary conditions representing convective and diffusive transport of the substrates glucose and hydrogen peroxide, as well as reaction kinetics of the bienzyme electrode. The main parameter investigated was the ratio of the two immobilised enzymes, with the aim …