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University of Massachusetts Amherst
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Persistent Radical Anion Polymers Based On Naphthalenediimide And A Vinylene Spacer, Sashi Debnath, Connor J. Boyle, Dongming Zhou, Bryan M. Wong, Kevin R. Kittilstved, Dhandapani Venkataraman
Persistent Radical Anion Polymers Based On Naphthalenediimide And A Vinylene Spacer, Sashi Debnath, Connor J. Boyle, Dongming Zhou, Bryan M. Wong, Kevin R. Kittilstved, Dhandapani Venkataraman
Chemistry Department Faculty Publication Series
Persistent n-doped conjugated polymers were achieved by doping the electron accepting PDNDIV and PFNDIVpolymers with ionic (TBACN) or neutral (TDAE) dopants. The great electron affinities, as indicated by the low LUMO levels of PDNDIV (−4.09 eV) and PFNDIV (−4.27 eV), facilitated the chemical reduction from either TBACN or TDAE. The low-lying LUMOs of the neutral polymers PDNDIV and PFNDIV were achieved by incorporation of vinylene spacers between the electron poor NDI units to increase the conjugation length without the use of an electron donor, and this was lowered further by an electron-withdrawing fluorinated N-substituent on the NDI moiety. The …
3' End Additions By T7 Rna Polymerase Are Rna Self-Templated, Distributive And Diverse In Character––Rna-Seq Analyses, Yasaman Gholamalipour, Aruni K. Mudiyanselage, Craig T. Martin
3' End Additions By T7 Rna Polymerase Are Rna Self-Templated, Distributive And Diverse In Character––Rna-Seq Analyses, Yasaman Gholamalipour, Aruni K. Mudiyanselage, Craig T. Martin
Chemistry Department Faculty Publication Series
Synthetic RNA is widely used in basic science, nanotechnology and therapeutics research. The vast majority of this RNA is synthesized in vitro by T7 RNA polymerase or one of its close family members. However, the desired RNA is generally contaminated with products longer and shorter than the DNA-encoded product. To better understand these undesired byproducts and the processes that generate them, we analyze in vitro transcription reactions using RNA-Seq as a tool. The results unambiguously confirm that product RNA rebinds to the polymerase and self-primes (in cis) generation of a hairpin duplex, a process that favorably competes with promoter driven …