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Qpcr Analysis Of Differential Gene Expression (Wet Lab), Raymond A. Enke
Qpcr Analysis Of Differential Gene Expression (Wet Lab), Raymond A. Enke
Ray Enke Ph.D.
This class tested protocol will guide students through the steps for the following activities:
- Analyze qPCR data to determine normalized gene expression for candidate genes identified from an RNA-Seq data set
- Set up additional or repeat experimental qPCR reactions
- Create a draft poster outlining the methods & data collected during the 6-week RNA-Seq analysis lab module
1st Strand Cdna Synthesis From Total Rna (Wet Lab), Raymond A. Enke
1st Strand Cdna Synthesis From Total Rna (Wet Lab), Raymond A. Enke
Ray Enke Ph.D.
This class tested protocol will guide students through the steps for the following activities:
• Construct a cDNA library representative of total cellular messenger RNAs
Dissection & Rna Isolation From Embryonic Chicken Retina (Wet Lab), Raymond A. Enke
Dissection & Rna Isolation From Embryonic Chicken Retina (Wet Lab), Raymond A. Enke
Ray Enke Ph.D.
This class tested protocol will guide students through the steps for the following activities:
- Dissect retina & cornea from developing chicken embryos
- Extract total cellular RNA from tissues for downstream gene expression analysis
Rna Gel & Spectrophotometer Analysis (Wet Lab), Raymond A. Enke
Rna Gel & Spectrophotometer Analysis (Wet Lab), Raymond A. Enke
Ray Enke Ph.D.
This class tested protocol will guide students through the steps for the following activities:
- Determine quantity & purity of total RNA using a spectrophotometer
- Determine the quality of total RNA using native agarose gel electrophoresis (no formaldehyde required)