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Fungi On White-Nose Infected Bats (Myotis Spp.) In Eastern Canada Show No Decline In Diversity Associated With Pseudogymnoascus Destructans (Ascomycota: Pseudeurotiaceae), Karen J. Vanderwolf, David Malloch, Donald F. Mcalpine Dec 2015

Fungi On White-Nose Infected Bats (Myotis Spp.) In Eastern Canada Show No Decline In Diversity Associated With Pseudogymnoascus Destructans (Ascomycota: Pseudeurotiaceae), Karen J. Vanderwolf, David Malloch, Donald F. Mcalpine

International Journal of Speleology

The introduction of the fungal pathogen Pseudogymnoascus destructans (Pd) to North America has stimulated research on the poorly known mycology of caves. It is possible that the introduction of Pd reduces the diversity of fungi associated with bats hibernating in caves. To test this hypothesis we examined the fungal assemblages associated with hibernating bats (Myotis spp.) pre- and post- white-nose syndrome (WNS) infection in eastern Canada using culture-dependent methods. We found the mean number of fungal taxa isolated from bats/hibernaculum was not significantly different between pre-infection (29.6 ± 6.1SD) and post-infection with WNS (32.4 ± 4.3). Although …


Cave Cyanobacteria Showing Antibacterial Activity, Vasiliki Lamprinou, Kyriaki Tryfinopoulou, Emmanuel N. Velonakis, Alkiviadis Vatopoulos, Smaragdi Antonopoulou, Elizabeth Fragopoulou, Adriani Pantazidou, Athena Economou-Amilli May 2015

Cave Cyanobacteria Showing Antibacterial Activity, Vasiliki Lamprinou, Kyriaki Tryfinopoulou, Emmanuel N. Velonakis, Alkiviadis Vatopoulos, Smaragdi Antonopoulou, Elizabeth Fragopoulou, Adriani Pantazidou, Athena Economou-Amilli

International Journal of Speleology

Cave Cyanobacteria - thriving in an ‘extreme’ environment with interesting species biodiversity - are supposed to be a potential source of bioactive compounds. Lipid extracts from pure cultures of two recently established Cyanobacteria from Greek caves, Toxopsis calypsus and Phormidium melanochroun, were used for antibacterial screening against human pathogenic bacteria (reference and clinical isolates). Antimicrobial Susceptibility testing for both taxa was carried out using the disc-diffusion (Kirby Bauer) method, while preliminary data applying the standard broth microdilution method for the determination of the Minimal Inhibitory Concentration (MIC) are given only for T. calypsus. Antibacterial activity was demonstrated against the …


Characterization And Fluorescence Of Yellow Biofilms In Karst Caves, Southwest Slovenia, Janez Mulec, Andreea Oarga-Mulec, Rok Tomazin, Tadeja Matos May 2015

Characterization And Fluorescence Of Yellow Biofilms In Karst Caves, Southwest Slovenia, Janez Mulec, Andreea Oarga-Mulec, Rok Tomazin, Tadeja Matos

International Journal of Speleology

Biofilms of different colours that colonize surfaces within karst caves represent a source of nutrients. They occur commonly and abundantly at sites with sediments, and close to seepages or underground rivers. Golden-yellow subaerial biofilms are particularly well observed because of their contrast with their surroundings, the characteristics of the pigment and recently, even more, due to the characteristics of light-emitting diode (LED) illumination. Yellow microbial biofilms were sampled from three caves in southwestern Slovenia, Dimnice, Križna jama and Sveta jama. The highest concentration of cultivable microbes (2.33×108 CFU/g) and the biggest number of identified bacteria (66.0%) were retrieved from …


Cyanobacteria, Algae And Microfungi Present In Biofilm From Božana Cave (Serbia), Slađana Popović, Gordana Subakov Simić, Miloš Stupar, Nikola Unković, Dragana Predojević, Jelena Jovanović, Milica Ljaljević Grbić Feb 2015

Cyanobacteria, Algae And Microfungi Present In Biofilm From Božana Cave (Serbia), Slađana Popović, Gordana Subakov Simić, Miloš Stupar, Nikola Unković, Dragana Predojević, Jelena Jovanović, Milica Ljaljević Grbić

International Journal of Speleology

Phototrophic microorganisms (cyanobacteria and algae) and microfungi, were identified from biofilm on the walls of the entrance of BožanaCavein west Serbia. Temperature, relative humidity and light intensity were measured, and chlorophyll a content determined. Light intensity differed from the entrance inwards. However, Chl a content was not proportional to light intensity, instead it was positively correlated to biofilm weight. Biofilm samples from two sites were also observed using a scanning electron microscope. Coccoid forms of cyanobacteria were abundant at the sampling site with the lowest light intensity, while members of the order Nostocales were predominant at the sampling site with …