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Evaluation Of Vascular Control Mechanisms Utilizing Video Microscopy Of Isolated Resistance Arteries Of Rats, Kathleen M. Lukaszewicz, Matthew J. Durand, Jessica R. C. Priestley, James R. Schmidt, L. Adrienne Allen, Aron M. Geurts, Julian H. Lombard
Evaluation Of Vascular Control Mechanisms Utilizing Video Microscopy Of Isolated Resistance Arteries Of Rats, Kathleen M. Lukaszewicz, Matthew J. Durand, Jessica R. C. Priestley, James R. Schmidt, L. Adrienne Allen, Aron M. Geurts, Julian H. Lombard
Physical Therapy Faculty Research and Publications
This protocol describes the use of in vitro television microscopy to evaluate vascular function in isolated cerebral resistance arteries (and other vessels), and describes techniques for evaluating tissue perfusion using Laser Doppler Flowmetry (LDF) and microvessel density utilizing fluorescently labeled Griffonia simplicifolia (GS1) lectin. Current methods for studying isolated resistance arteries at transmural pressures encountered in vivo and in the absence of parenchymal cell influences provide a critical link between in vivo studies and information gained from molecular reductionist approaches that provide limited insight into integrative responses at the whole animal level. LDF and techniques to selectively identify arterioles and …
Ventilation And Neurochemical Changes During Μ-Opioid Receptor Activation Or Blockade Of Excitatory Receptors In The Hypoglossal Motor Nucleus Of Goats, Thomas M. Langer, Suzanne E. Neumueller, Emma Crumley, Nicholas J. Burgraff, Sawan Talwar, Matthew R. Hodges, Lawrence Pan, Hubert V. Forster
Ventilation And Neurochemical Changes During Μ-Opioid Receptor Activation Or Blockade Of Excitatory Receptors In The Hypoglossal Motor Nucleus Of Goats, Thomas M. Langer, Suzanne E. Neumueller, Emma Crumley, Nicholas J. Burgraff, Sawan Talwar, Matthew R. Hodges, Lawrence Pan, Hubert V. Forster
Physical Therapy Faculty Research and Publications
Neuromodulator interdependence posits that changes in one or more neuromodulators are compensated by changes in other modulators to maintain stability in the respiratory control network. Herein, we studied compensatory neuromodulation in the hypoglossal motor nucleus (HMN) after chronic implantation of microtubules unilaterally (n = 5) or bilaterally (n = 5) into the HMN. After recovery, receptor agonists or antagonists in mock cerebrospinal fluid (mCSF) were dialyzed during the awake and non-rapid eye movement (NREM) sleep states. During day studies, dialysis of the µ-opioid inhibitory receptor agonist [d-Ala2, N-MePhe4, Gly-ol]enkephalin (DAMGO; 100 µM) decreased …
Effects On Breathing Of Agonists To Μ-Opioid Or GabaA Receptors Dialyzed Into The Ventral Respiratory Column Of Awake And Sleeping Goats, Thomas M. Langer Iii, Suzanne E. Neumueller, Emma Crumley, Nicholas J. Burgraff, Sawan Talwar, Matthew R. Hodges, Lawrence Pan, Hubert V. Forster
Effects On Breathing Of Agonists To Μ-Opioid Or GabaA Receptors Dialyzed Into The Ventral Respiratory Column Of Awake And Sleeping Goats, Thomas M. Langer Iii, Suzanne E. Neumueller, Emma Crumley, Nicholas J. Burgraff, Sawan Talwar, Matthew R. Hodges, Lawrence Pan, Hubert V. Forster
Physical Therapy Faculty Research and Publications
Pulmonary ventilation (V̇I) in awake and sleeping goats does not change when antagonists to several excitatory G protein-coupled receptors are dialyzed unilaterally into the ventral respiratory column (VRC). Concomitant changes in excitatory neuromodulators in the effluent mock cerebral spinal fluid (mCSF) suggest neuromodulatory compensation. Herein, we studied neuromodulatory compensation during dialysis of agonists to inhibitory G protein-coupled or ionotropic receptors into the VRC. Microtubules were implanted into the VRC of goats for dialysis of mCSF mixed with agonists to either μ-opioid (DAMGO) or GABAA (muscimol) receptors. We found: (1) V̇I decreased during unilateral but increased during …
State-Dependent And -Independent Effects Of Dialyzing Excitatory Neuromodulator Receptor Antagonists Into The Ventral Respiratory Column, Thomas M. Langer Iii, Suzanne E. Neumueller, Emma Crumley, Nicholas J. Burgraff, Sawan Talwar, Matthew R. Hodges, Lawrence Pan, Hubert V. Forster
State-Dependent And -Independent Effects Of Dialyzing Excitatory Neuromodulator Receptor Antagonists Into The Ventral Respiratory Column, Thomas M. Langer Iii, Suzanne E. Neumueller, Emma Crumley, Nicholas J. Burgraff, Sawan Talwar, Matthew R. Hodges, Lawrence Pan, Hubert V. Forster
Physical Therapy Faculty Research and Publications
Unilateral dialysis of the broad-spectrum muscarinic receptor antagonist atropine (50 mM) into the ventral respiratory column [(VRC) including the pre-Bötzinger complex region] of awake goats increased pulmonary ventilation (V̇i) and breathing frequency (f), conceivably due to local compensatory increases in serotonin (5-HT) and substance P (SP) measured in effluent mock cerebral spinal fluid (mCSF). In contrast, unilateral dialysis of a triple cocktail of antagonists to muscarinic (atropine; 5 mM), neurokinin-1, and 5-HT receptors does not alter V̇i or f, but increases local SP. Herein, we tested hypotheses that 1) local compensatory 5-HT and SP responses to 50 mM atropine …