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Would You Recognize Lupus?, Sydney C. Smith, Maegan Simms, Barbara Brown

Dental Hygiene Student Scholarship

Systemic Lupus Erythematosus, or SLE, is a chronic autoimmune disease which significantly affects various organs of the body and the oral cavity. According to various studies, SLE can cause an increased risk of periodontitis, fungal infections, and dental caries within the oral cavity. Periodontitis is an inflammatory condition mediated by an infectious etiology which affects the supporting tissues of the periodontium and alveolar bone. With SLE being an inflammatory condition as well, recent studies have emerged hypothesizing the possible association between SLE and periodontitis. Other effects on the oral cavity such as fungal infections including lichen planus and angular cheilitis …

Proteomic Approach For Extracting Cytoplasmic Proteins From Streptococcus Sanguinis Using Mass Spectrometry, Fadi Elrami, Kristina Nelson, Ping Xu

Philips Institute for Oral Health Research Publications

Streptococcus sanguinis is a commensal and early colonizer of oral cavity as well as an opportunistic pathogen of infectious endocarditis. Extracting the soluble proteome of this bacterium provides deep insights about the physiological dynamic changes under different growth and stress conditions, thus defining “proteomic signatures” as targets for therapeutic intervention. In this protocol, we describe an experimentally verified approach to extract maximal cytoplasmic proteins from Streptococcus sanguinis SK36 strain. A combination of procedures was adopted that broke the thick cell wall barrier and minimized denaturation of the intracellular proteome, using optimized buffers and a sonication step. Extracted proteome was quantitated …

A Systems Biology Approach For Predicting Essential Genes And Deciphering Their Dynamics Under Stress In Streptococcus Sanguinis, Fadi El-Rami

Theses and Dissertations

Infectious diseases are the top leading cause of death worldwide. Identifying essential genes, genes indispensable for survival, has been proven indispensable in defining new therapeutic targets against pathogens, major elements of the minimal set genome to be harnessed in synthetic biology, and determinants of evolutionary relationships of phylogenetically distant species. Thus, essentiality studies promise valuable revenues that can decipher much of biological complexities.

Taking advantage of the available microbial sequences and the essentiality studies conducted in various microbial models, we proposed a framework for the prediction of essential genes based on our experimentally verified knowledge of the pathways involved in …