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Trna Profiling Of Mesenchymal Stem Cell Exosome, Khin Mimi San
Trna Profiling Of Mesenchymal Stem Cell Exosome, Khin Mimi San
Theses and Dissertations
Background: Exosomes have great potential in regenerative medicine through the
transfer of their bioactive cargos, such as RNA. tRF RNA and tiRNA are tRNAderived
non-coding RNA. Here, we sought to identify the tRF/tiRNA profile in human
mesenchymal stem cell (hMSC) exosomes. Methods: Bone marrow hMSCs were
cultured with/without osteogenic differentiation medium and exosomes were
harvested. RNA was extracted from: 1) control cells (Cell-NT); 2) control exosomes
(EXO-NT); 3) differentiated cells (Cell-OM); 4) exosomes produced by differentiated
cells (EXO-OM). RNA was sequenced to profile the small RNA with a focus on
tRF/tiRNA. Results: tRF/tiRNA was highly enriched in hMSC exosomes. Less …
Directing Mesenchymal Stem Cells For Periodontal Regeneration, Charles Stoianovici
Directing Mesenchymal Stem Cells For Periodontal Regeneration, Charles Stoianovici
Theses and Dissertations
Background: Directing autogenous Mesenchymal Stem Cell (MSC) to defect sites has a great promise in bone regeneration. We designed a MSC specific, bone affinity peptide (E7HA7) by conjugating E7 with a polyglutamate hydroxyapatite (HA) binding motif. We sought to characterize the in-vivo releasing pattern and bioactivity of E7HA7. Methods: HA discs were coated with fluorescent labeled peptides E7HA7, E7HA2 or E7 were subcutaneously implanted in Sprague Dawley rats. In an ectopic bone formation model was used to test the in-vivo bioactivity of E7HA7 conjugated to DBM. Results: E7HA7 showed slower peptide release from scaffolds in comparison to other groups, being …