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Characterization Of 50s Ribosomal Subunit Assembly Inhibition In Erythromycin Treated Escherichia Coli Cells., Jerry Edward Usary

Electronic Theses and Dissertations

Erythromycin has long been recognized for its ability to inhibit protein synthesis by interfering with mRNA translation on the bacterial ribosome. We have recently shown that erythromycin also inhibits the assembly of the 50S ribosomal subunit in growing bacterial cells. The nature of this assembly inhibition has been investigated using ³H-uridine pulse-chase labeling of control and erythromycin treated E. coli cells.

Subunit assembly was examined by sucrose gradient centrifugation of labeled cell lysates. Normal assembly kinetics of subunit assembly were observed in control cells at 37°C. Formation of the 30S subunit was completed by 7.5 minutes and assembly of …

Perceived Barriers To The Teaching Of Critical Thinking Skills By Nursing Faculty In Generic Bsn Programs In Tennessee, Renee C. Shell

Electronic Theses and Dissertations

Teaching critical thinking is a national education goal. The ability to think critically is considered an essential skill of nursing graduates and is a necessary component of competent nursing practice. Therefore, nursing programs must address critical thinking for accreditation. The literature reports that teachers in all areas of education are having difficulties infusing critical thinking teaching strategies into their current teaching practices and critical thinking is lacking in new nursing graduates. This research study sought to identify barriers to the implementation of critical thinking teaching strategies as perceived by nursing faculty in generic, BSN programs in Tennessee. Data were collected …