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Late Stages Of Hematopoiesis And B Cell Lymphopoiesis Are Regulated By Α-Synuclein, A Key Player In Parkinson's Disease, Wenbin Xiao, Afshin Shameli, Clifford V. Harding, Howard J. Meyerson, Robert W. Maitta
Late Stages Of Hematopoiesis And B Cell Lymphopoiesis Are Regulated By Α-Synuclein, A Key Player In Parkinson's Disease, Wenbin Xiao, Afshin Shameli, Clifford V. Harding, Howard J. Meyerson, Robert W. Maitta
Faculty Scholarship
α-Synuclein plays a crucial role in Parkinson's disease and dementias defined as synucleinopathies. α-Synuclein is expressed in hematopoietic and immune cells, but its functions in hematopoiesis and immune responses are unknown. We utilized α-synuclein(-/-) (KO) mice to investigate its role in hematopoiesis and B cell lymphopoiesis. We demonstrated hematologic abnormalities including mild anemia, smaller platelets, lymphopenia but relatively normal early hematopoiesis in KO mice compared to wild-type (WT) as measured in hematopoietic stem cells and progenitors of the different cell lineages. However, the absolute number of B220(+)IgM(+) B cells in bone marrow was reduced by 4-fold in KO mice (WT: …
Heterogeneous Red Blood Cell Adhesion And Deformability In Sickle Cell Disease, Yunus Alapan, Jane A. Little, Umut A. Gurkan
Heterogeneous Red Blood Cell Adhesion And Deformability In Sickle Cell Disease, Yunus Alapan, Jane A. Little, Umut A. Gurkan
Faculty Scholarship
We present a microfluidic approach that allows simultaneous interrogation of RBC properties in physiological flow conditions at a single cell level. With this method, we studied healthy hemoglobin A (HbA) and homozygous sickle hemoglobin (HbS) containing RBCs using whole blood samples from twelve subjects. We report that HbS-containing RBCs are heterogeneous in terms of adhesion and deformability in flow.
Steady Increment Of Immature Platelet Fraction Is Suppressed By Irradiation In Single-Donor Platelet Components During Storage, Hong Hong, Wenbin Xiao, Robert W. Maitta
Steady Increment Of Immature Platelet Fraction Is Suppressed By Irradiation In Single-Donor Platelet Components During Storage, Hong Hong, Wenbin Xiao, Robert W. Maitta
Faculty Scholarship
Circulating immature platelet fraction (IPF) reflects real-time thrombopoiesis and correlates with platelet recovery from thrombocytopenic presentations. To understand the dynamics of IPF in platelet transfusions, we quantified the %-IPF in single-donor platelet components (SDP) during prolonged storage. %-IPF significantly increased from baseline by day 5 post-donation. Absolute IPF counts (A-IPC) had similar significant increments. However, gamma-irradiation suppressed the increments of %-IPF and A-IPC by >50%. Ultrastructural analysis of SDP units at day 10 showed well preserved morphology of immature platelets. Our findings suggest that IPF might actively expand ex-vivo and may have a longer shelf life than their mature counterparts. …