Digital Commons Network^™

The Role Of Zinc In Dihydroorotase, Pamela S. Gilchrist

Dissertations and Theses

Dihydroorotase (4,4—dihydroorotic acid amidolyase, EC 3.5.2.3.) which catalyzes the reversible cyclization of N-carbamyl-L-aspartate to L-dihydroorotate has been purified from orotate-grown Clostridium oroticum. The enzyme is stable in 0.3 M sodium chloride and 10 µ ZnSO₄. Sodium dodecyl sulfate gel electrophoresis indicates the enzyme to be composed of two identical subunits each with a molecular weight of 58,000 + 6000. Dihydroorotase is shown to be a zinc-containing metalloenzyme with 2 g atoms of zinc per 58,000 g of protein. The role of zinc in dihydroorotase is discussed.

Biochemical Description Of A Lava Tube Lake In Southeast Oregon, John Edward Palmer

Dissertations and Theses

A Pleistocene lava tube cave in southeast Oregon has a seasonally fluctuating lake occupying the last 1200 to 2000 feet. Three endemic invertebrate species inhabit the lake: Kenkia rynchida Hyman; Asellus sp.; and Stygobromus hubbsi Shoemaker. Little is known however, about their environment. The purpose of this study was to describe various physical and chemical parameters of Malheur Cave Lake.

Effects Of Antifibroblast Antiserum On Cells Derived From Fibroblast Outgrowth Of Human Prostatic Tissues, Eva Shang-Lian King

Dissertations and Theses

The purpose of this investigation was to provide pure cultures of normal human prostatic epithelium free of fibroblasts in order to study malignant conversion by chemical carcinogens. Normal epithelial cells were needed because this was the cell type implicated in prostatic malignancies of human subjects. Unfortunately fibroblasts grew faster than epithelial cells so that cultures were always overgrown with connective tissue elements. It was considered important to find a method which would eliminate fibroblasts so that normal epithelial cells could grow out in pure culture.

Interactions In The Frequencies Of Electric Organ Discharge By Eigenmannia Virescens (Sternopygidae, Pisces) During Social Behavior, Philip Gaddis

Dissertations and Theses

The extent to which individuals of Eigenmannia virescens modulate their electric organ discharge frequencies to accommodate the frequencies of others of the same species during social interactions was investigated. Recordings of the fish's discharge frequencies, taken with Tektronix 3L5 and 5L4N spectrum analyzers, showed that, although movements of up to 60 Hz in a day may be made, the frequency movements would be made more or less synchronously by all fish in the group. An apparent preference for, and a tendency of the fish to follow one another in frequency, at frequency ratios of 2:3 (a musical fifth), 3:4 (fourth), …

The Distribution Of The Deer Mouse, Peromyscus Maniculatus, On The Oregon Side Of The Columbia River Gorge, Ronald P. Neilson

Dissertations and Theses

A study of the biogeography of Peromyscus maniculatus was undertaken in order to ascertain a few of the environmental parameters important in defining the distribution of this species and how the species in turn has adapted to these parameters. The Columbia Gorge was chosen as it presents a climatic gradient from maritime to continental with very little elevation gain. Changes along this gradient in topography, soils and vegetation community structure are discussed.

A Biochemical Analysis Of Viscin From Arceuthobium Tsugense, Peter J. Paquet

Dissertations and Theses

The viscin coating of seeds from Arceuthobium tsugense was analyzed histochemically. The results indicated that the viscin is composed of two components. The first is a muculagenous pectic compound which surrounds the entire seed and accounts for a number of observed phenomenon such as stickiness, water holding capacity and the ability to undergo repeated drying and rehydration. The second component is made up of a large number of cellulose strands which are attached to the endocarp and run throughout the pectic coating. Scanning electron micrographs show that they are thought to be important in maintaining the integrity of the pectic …

The Morphology Of The Eye Of The Purple Shore Crab, Hemigrapsus Nudus, Sharon E. Heisel

Dissertations and Theses

A structural analysis of the compound eye of Hemigrapsus nudus expands the basis of functional analysis of decapod Crustacean eyes. Contradictory evidence for disintegration of rhabdomeric microvilli in the absence of light prompted observation of H. nudus eyes after 146 days in darkness.

Eyes were fixed with formalin and glutaraldehyde and post fixed with osmium tetroxide for electron and light microscopy. Light- dark-adapted eyes were also observed with hot water fixation and paraffin embedment.

The eye of H. nudus is typical of decapod Crustacean compound eyes. Corneagenous cells underlie the transparent cuticle cone cells secrete the composite crystalline cone which …