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Isolation Of Mesenchymal Stem Cells Derived From Adult Bone Marrow And Umbilical Cord Blood And Their Potential To Differentiate Into Osteoblasts, Andrew P. Pacitti
Isolation Of Mesenchymal Stem Cells Derived From Adult Bone Marrow And Umbilical Cord Blood And Their Potential To Differentiate Into Osteoblasts, Andrew P. Pacitti
Theses and Dissertations
The demand for treatment strategies of musculoskeletal tissues is continuously growing, especially considering the increasing number of elderly people with degenerative diseases of the skeletal system. Despite major strides in the field of bone regenerative medicine during the years, current therapies, such as bone grafts, still have several limitations. Multipotent stem cells, such as mesenchymal stem cells (MSCs) are promising candidates for tissue repair because of their differentiation potential and their capacity to undergo extensive replication. However, isolating a homogeneous population of MSCs from multiple sources is an area that needs to be addressed. Also, the knowledge regarding the mechanisms …
Acquisition And Retention Of Bacterial Spores (Bacillus Atrophaeus) By Eight Insect Species, Kieron Marie Torres
Acquisition And Retention Of Bacterial Spores (Bacillus Atrophaeus) By Eight Insect Species, Kieron Marie Torres
Theses and Dissertations
Acquisition and retention of spores of an anthrax surrogate, Bacillus atrophaeus Nakamura ("BG") were evaluated in eight insect species. Species included: house cricket (Acheta domesticus L.), German cockroach (Blatella germanica L.), common house fly (Musca domestics L.), blue bottle fly (Calliphora vomitoria L.), hairy rove beetle Creophilus maxillosus L.), yellow mealworm (Tenebrio molitor L.), common paper wasps (Polistes exclamans exclamans Viereck), red paper wasps (Polistes Carolina L.), red harvester ant (Pogonymyrmex barbatus Smith). Individual insects were offered BG-treated food and sacrificed at specified time intervals following one, two or three meals. Resulting samples were surface-washed five consecutive times then homogenized …
Genetic Assessment Of Rare Blackbanded Sunfish (Enneacanthus Chaetodon) Populations In Virginia, Diana Marie Kercher
Genetic Assessment Of Rare Blackbanded Sunfish (Enneacanthus Chaetodon) Populations In Virginia, Diana Marie Kercher
Theses and Dissertations
Enneacanthus chaetodon, the blackbanded sunfish, has become increasingly rare throughout its distribution in the Eastern United States. In Virginia, E. chaetodon maintains an endangered status and individuals persist in six populations. Mitochondrial DNA (mtDNA) and microsatellite data were assessed to determine the genetic characters and gene diversity of the Virginia populations. The results of these analyses were then compared to five additional populations; four from New Jersey and one from North Carolina that were known to have relatively good fitness and were not impacted severely by habitat alteration. The results of this study are relevant to selection of proper management …
Characterization Of The Rank Ligand Positive Giant Cell Found In The Interfacial Membrane, Patrick Emerson Jones
Characterization Of The Rank Ligand Positive Giant Cell Found In The Interfacial Membrane, Patrick Emerson Jones
Theses and Dissertations
Aseptic osteolysis is a major complication to total joint arthroplasty requiring several thousand people a year to have to undergo revisions of their joint prosthesis. The formation of the interfacial membrane has been associated with aseptic osteolysis leading to the failure of all types of total joints. Recent evidence suggests that RANKL, a potent activator of bone reabsorption, is present in the interfacial membrane. Prior research in this laboratory to determine the source of RANKL in the interfacial membrane has revealed the presence of intense areas of RANKL concentration in the membrane. These areas of RANKL concentration correspond to multiple …
Stretch-Dependant Tonic Force Maintenance In Rabbit Epigastric Artery, Krystina Michelle Berg
Stretch-Dependant Tonic Force Maintenance In Rabbit Epigastric Artery, Krystina Michelle Berg
Theses and Dissertations
The contractile state of vascular smooth muscle (VSM) plays a key role in blood pressure regulation. Abnormal VSM contractility characterizes hypertension and understanding the regulatory mechanisms of VSM may provide some insights to specific treatment of hypertension. Upon muscle stimulation, Ca2+, myosin light chain (MLC) phosphorylation, crossbridge cycling rates and force increase to high levels, but with time, force is maintained while all other parameters of muscle activation fall to low levels. Thus, contraction is divided temporally into early (phasic) and late (tonic) phases, as determined by the underlying regulatory mechanism. Muscles with more phasic characteristics have a higher peak …
Functional Consequences Of Matrix Metalloproteinase-1 Over-Expression In Human Gliomas, Emily Mullet
Functional Consequences Of Matrix Metalloproteinase-1 Over-Expression In Human Gliomas, Emily Mullet
Theses and Dissertations
Malignant brain tumors are among the deadliest of human cancers. Despit recent advancements in conventional therapies, glioblastomas remain incurable, largel y due to their ability to invade surrounding tissue. Matrix metalloproteinases are thought to contribute to the invaseive phenotype of human gliomas. Absent in normal brain, matrix metalloproteinase-1 (MMP-1) has been shown to be present in gliomas, and in particular in glioblastoma multiforme (GBM). To begin to examine the role of MMP-1 in these tumore, two human glioma cell lines were stably transfected with MMP-1 cDNA. Confirmation of MMP-1 over-expression in these cells was achieved through real-time PCR and Western …
Novel Cancer Therapeutics, The Generation Of Ros, And Cell Survival, Clint Mitchell
Novel Cancer Therapeutics, The Generation Of Ros, And Cell Survival, Clint Mitchell
Theses and Dissertations
The impact of Ad.mda-7 on the survival of renal cell carcinoma lines (RCC), primary renal epithelial cells, glioblastoma multiforme lines (GBM), and primary rodent astrocytes is unknown. The present studies examine whether the GST fusion protein, GST-MDA-7, and the adenovirus, Ad.mda-7, altered the growth and survival of the A498 and UOK121N RCC lines or radiosensitized GBM, respectively. Due to previous findings that the RCC lines, but not primary renal epithelial cells, were resistant to type 5 adenoviral infection, we used purified GST-MDA-7 protein to show that GST-MDA-7, but not GST, caused a dose-dependent reduction in A498 and UOK121N proliferation but …
Modulation Of The Serotonin Reuptake Transporter In Raw264.7 Macrophages, Sienna Marie Arenas Malubay
Modulation Of The Serotonin Reuptake Transporter In Raw264.7 Macrophages, Sienna Marie Arenas Malubay
Theses and Dissertations
Serotonin (5-HT) plays an important role as both a neurotransmitter and animmune modulator. The serotonin reuptake transporter (SERT) clears the extracellular space of 5-HT, which decreases the effects of 5-HT on target cells. This study demonstrated that the RAW264.7 macrophage cell line expresses SERT function, measured by assays of 3H-5HT uptake. The 5-HT uptake in RAW264.7 macrophages was more than 10-fold that of peritoneal macrophages, indicating that these cells are an excellent model for studying regulation of the SERT. Activation of macrophages with lipopolysaccharide (LPS) increased SERT activity in a time- and concentration-dependent manner and Western blots indicate that the …
Genetic Variation Among Geographically Disparate Yellow Perch Broodstock Populations, Chandler Brooke Givens
Genetic Variation Among Geographically Disparate Yellow Perch Broodstock Populations, Chandler Brooke Givens
Theses and Dissertations
As a prelude to strain selection for domestication and future marker assisted selection, genetic variation revealed by microsatellite DNA was evaluated in yellow perch, Perca flavescens, from four wild North American populations collected in 2003-2004 (Maine, ME; New York, NY; North Carolina, NC; and Pennsylvania, PA), and two captive populations (Michigan, MI; Ohio, OH). For the loci examined, levels of heterozygosity ranged from He = 0.04 to 0.88, genetic differentiation was highly significant among all population pairs, and effective migration ranged from low (Nem = 0.3) to high (Nem = 4.5). Deviation from Hardy-Weinberg 1equilibrium was regularly observed indicating significant …
Atm-Dependent Erk Signaling In Response To Dna Double Strand Breaks, Ashraf Khalil
Atm-Dependent Erk Signaling In Response To Dna Double Strand Breaks, Ashraf Khalil
Theses and Dissertations
Ionizing radiation (IR) triggers many signaling pathways stemming from DNA damage, and, independently, from extra-nuclear events. To generate radio-mimetic DNA double-strand breaks (DSBs) without and minimizing the effects on extra-nuclear radiation targets, human (p53+) glioma and carcinoma cells containing bromodeoxyuridine (BrdU)- substituted DNA were treated with Hoechst 33258 followed by long wave-length UV (UV-A) (BrdU photolysis). BrdU photolysis resulted in well-controlled, dose-dependent generation of DSBs equivalent to 0.2 - 20 Gy of IR, as detected by pulse-field gel electrophoresis, accompanied by dose-dependent H2AX phosphorylation at ser-139 and ATM phosphorylation at ser-1981, indicating ATM activation. Furthermore, BrdU photolysis increased phosphorylation of …
Phospholipase A2 Induced Monocyte Chemotaxis To Apoptotic Cells, Kwasi Karikari
Phospholipase A2 Induced Monocyte Chemotaxis To Apoptotic Cells, Kwasi Karikari
Theses and Dissertations
Apoptosis is a form of programmed cell death that is essential in such processes as organ and tissue remodeling and maturation of hematopoietic cells. The clearance of apoptotic cells is essential to prevent autoimmune responses to sequestered antigens. This process is mediated by phagocytes of the monocyte lineage. Before phagocytosis can occur, macrophages must be recruited to the apoptotic cells through chemotaxis. Products of the reaction catalyzed by the phospholipases A2 (PLA2) have been shown to induce monocyte chemotaxis either directly or indirectly. Some investigators have implicated a cytosolic calcium-independent PLA2 (iPLA2) in the production of these products during apoptosis. …
Monocyte Modulation Of Disease Pathogenesis And Progression In Localized Aggressive Periodontitis, Chu Ri Shin
Monocyte Modulation Of Disease Pathogenesis And Progression In Localized Aggressive Periodontitis, Chu Ri Shin
Theses and Dissertations
Localized Aggressive Periodontitis (LAgP) is an aggressive, early onset form of periodontitis characterized by a unique myeloid cell phenotype. In addition to its bacterial origin, the unique phenotype of the myeloid cell contributes to disease pathogenesis and progression through mechanisms mediating host inflammatory and immune responses. LAgP monocytes synthesize increased levels of the potent proinflammatory lipid mediator, Prostaglandin E2 (PGE2), preferentially differentiate into dendritic cells, and lead to increased IgG2 production. In addition, levels of Platelet Activating Factor (PAF) have shown to be elevated in the gingival tissue and gingival crevicular fluid of subjects with periodontitis. The aim of this …
Novel Characteristics Of Murine Bone Marrow-Derived Macrophages And Human Macrophage-Like Cells, George Tharwat Georges
Novel Characteristics Of Murine Bone Marrow-Derived Macrophages And Human Macrophage-Like Cells, George Tharwat Georges
Theses and Dissertations
These studies provide evidence for novel properties of macrophages derived from bone marrow stem cells. In study 1, treatment of activated mouse bone marrow-derived macrophages (BMM) with either catecholamine synthesis inhibitors (α-methyl-para-tyrosine and fusaric acid) or the β2 adrenergic receptor antagonist ICI 118,551 demonstrated that BMM produce catecholamines. The catecholamines modulated macrophage cytokine production through autocrine actions on adrenergic receptors. In study II, undifferentiated human bone marrow cells were incubated in 30% mouse L929 fibroblast conditioned medium and generated adherent cells within three days. The cells were clearly identifiable as macrophages based on surface proteins and phagocytic activity but produced …
Characterization Of The Ftf/Hnf-4 Sites Within The 7alpha- And The 12alpha-Hydroxylase Promoters Involved In The Bile Acid-Mediated Transcription Of Their Regulation, Preeti Pramanik
Theses and Dissertations
Bile acids regulate their own synthesis through a feedback regulatory mechanism of mainly two enzymes in the classic pathway, the 7α-hydroxylase and the 12α-hydroxylase. In the early 1990's it was shown that the regulatory responses of 7α-hydroxylase are mediated at the transcriptional level and since then many positive and negative transcription factors that mediate regulatory response have been identified. An important finding was that the transcription factors regulating the expression of 7α- and 12α-hydroxylase genes are nuclear receptors.One of the first nuclear receptors identified to play a role in the transcription of the 7α-hydroxylase gene was HNF-4 since then many …
Role Of Mdm2 In Cell Growth Regulation, Rebecca Anne Frum
Role Of Mdm2 In Cell Growth Regulation, Rebecca Anne Frum
Theses and Dissertations
MDM2 has been shown to induce G0-Gl/S phase arrest. To determine the cell cycle step targeted by MDM2, flow cytometry was employed to detect induction of events during the G1-S phase transition in MDM2-arrested cells. MDM2 overexpression does not prevent expression of cyclin D, cyclin D-CDK mediated phosphorylation of Rb or cyclin E in normal, immortal or tumor-derived cells. However, MDM2 down-regulates cyclin A expression specifically in normal cells, which is associated with G1 arrest. The domain of MDM2 capable of this function is located within its N-terminal 58-109 amino acids. To down-regulate cyclin A, MDM2 requires a functional pl6/Brg …
Degenerate Oligonucleotide Primed - Polymerase Chain Reaction Evaluation And Optimization To Improve Downstream Forensic Str Analysis Of Low Quality/Low Quantity Dna, Lindsay Paige Thompson
Degenerate Oligonucleotide Primed - Polymerase Chain Reaction Evaluation And Optimization To Improve Downstream Forensic Str Analysis Of Low Quality/Low Quantity Dna, Lindsay Paige Thompson
Theses and Dissertations
When forensic biological samples yield low quality/low quantity DNA, thecurrent STR analysis methods do not generate acceptable profiles. Whole genomeamplification can be used to pre-amplify the entire genome for downstream analyses. A commercially available kit for DOP-PCR, a form of WGA, is currently being used in the clinical for downstream single locus targets. Forensic analyses utilize a multiplex amplification. This study determined that the "home brew" created by our lab performs the same as the commercially available kit. Future optimization studies of DOP-PCR can utilize this "home brew". Additionally, this research determined that a 10 second increase in electrokinetic injection …
The Effects Of Hemoglobin-Based Oxygen Carriers On Mean Arterial Pressure, Arteriolar Diameter, And Nitric Oxide In The Microcirculation, Veronique C. Hionis
The Effects Of Hemoglobin-Based Oxygen Carriers On Mean Arterial Pressure, Arteriolar Diameter, And Nitric Oxide In The Microcirculation, Veronique C. Hionis
Theses and Dissertations
In the US today, blood transfusion is safer than ever. Nevertheless, the century-old quest for a suitable blood substitute persists. The elimination of unwanted side effects, especially transfusion-transmitted diseases, the problems and high cost factor involved in collecting and storing human blood, the pending worldwide shortages, and the need for compatibility testing are the driving forces contributing towards the development of blood substitutes. The leading research is focusing on hemoglobin-based oxygen carriers (HBOCs), which are limited in clinical application due to the pressor effect they induce. In this study, the mechanisms through which HBOCs affect mean arterial pressure (MAP), arteriolar …
The Effect Of Adding Drag-Reducing Polymers To Resuscitation Fluid During Hemorrhagic Shock On Skeletal Muscle Microcirculation, Geoffrey C. Alexander
The Effect Of Adding Drag-Reducing Polymers To Resuscitation Fluid During Hemorrhagic Shock On Skeletal Muscle Microcirculation, Geoffrey C. Alexander
Theses and Dissertations
Previous studies have shown an increase in survival when a minute amount of drag-reducing polymers were added to a resuscitation fluid. The purpose of this investigation was to examine the effect of adding a minute amount of the drag-reducing polymer polyethylene glycol to a resuscitation fluid, on the microcirculation of skeletal muscle during a volume-controlled hemorrhage model. The spinotrapezius muscle in twelve male Sprague Dawley rats was exteriorized for microvascular measurements of the arterioles. The diameters of the three levels of arterioles, interstitial fluid PO2, and RBC velocity in the feed arteriole were measured. Flow in the feed arteriole was …
Calcium/Calmodulin Dependent Protein Kinase Type-Ii Associates With Flightless-I To Influence Its Nuclear Localization, Matthew Edward Seward
Calcium/Calmodulin Dependent Protein Kinase Type-Ii Associates With Flightless-I To Influence Its Nuclear Localization, Matthew Edward Seward
Theses and Dissertations
Ca2+/calmodulin-dependent protein kinase type-II (CaMK-II) is a Ser/Thr protein kinase regulated by Ca2+ and Calmodulin. It is a highly conserved and broadly expressed enzyme and has a unique structure and dynamic regulation. It has the ability to remain active in the absence of Ca 2+ as a result of Ca2+ dependent autophosphorylation. CaMK-II phospliorylates proteins involved in neurotransmitter secretion, long term potentiation, cytoskeletal dynamics, gene transcription, and cell motility. To support existing and identify new intracellular roles of CaMK-II, potential binding partners were identified. This was accomplished by transfecting and purifying "FLAG-tagged" CaMK-II's (α, βE, δC, and δE). CaMK-II associated …
Early Mediators Of Cutaneous Ischemia Reperfusion Injury: A Mouse Model, Christopher Rogers Pruitt
Early Mediators Of Cutaneous Ischemia Reperfusion Injury: A Mouse Model, Christopher Rogers Pruitt
Theses and Dissertations
Chronic tissue injuries present an enormous problem to both patients and healthcare professionals, and yet little is definitively known as to the underlying pathophysiology. While there are numerous comorbidities associated with these wounds, a unifying theme has been proposed to be the repetitive incidence of ischemia reperfusion injury.A mouse model of cyclic ischemia reperfusion was employed to examine the initiation events in the pathology of chronic wounds. Mice were subjected to eight hours of magnetic skin compression via four cycles of two-hours ischemia and thirty minutes of reperfusion. The presence of neutrophil markers of oxidative stress and inflammation such as …
Effect Of Nitric Oxide On Oxygen Consumption Of Skeletal Muscle, Christina Lyn Cox
Effect Of Nitric Oxide On Oxygen Consumption Of Skeletal Muscle, Christina Lyn Cox
Theses and Dissertations
Mammalian cells require a continuous and sufficient supply of oxygen to carry out their functions. The oxygen pathway has an overall direction taking O2 from the air to the mitochondria, which is a result of the mitochondrial O2 consumption (VO2) NO various effects on the mitochondria: at low concentrations for short periods NO specifically and irreversibly inhibits cytochrome c and reversibly inhibits cytochrome c oxidase, to decrease VO2. Thus, NO can modulate VO2 of skeletal muscle. The purpose of the present study was to measure VO2 of the rat spinotrapezius muscle under conditions of altered NO. The methods used provide …
Autocrine Effects Of Catecholamines On Macrophage Release Of Interleukin-6 (Il-6), Shaunta D. Poe
Autocrine Effects Of Catecholamines On Macrophage Release Of Interleukin-6 (Il-6), Shaunta D. Poe
Theses and Dissertations
Effects of norepinephrine (NE) on macrophage cytokine release are complex because the cells have both α2 and β2 adrenergic receptors, which mediate opposing actions. Furthermore, β2-adrenergic agonists are reported to have both stimulatory and inhibitory effects on interleukin-6 (IL-6). This study was designed to clarify the autocrine role of macrophage-derived NE on IL-6 production in activated peritoneal macrophages. Effects of NE on IL-6 production in the RAW264.7 macrophage cell-line also were investigated. Treatment of activated peritoneal macrophages with endotoxin, the α2-adrenergic antagonists yohimbine or RS79948 revealed that the α2-adrenergic receptor mediates a stimulatory autocrine action of catecholamines on IL-6 production. …
Invasion Genetics Of The Blue Catfish (Ictalurus Furcatus) Range Expansion Into Large River Ecosystems Of The Chesapeake Bay Watershed, Colleen Beth Higgins
Invasion Genetics Of The Blue Catfish (Ictalurus Furcatus) Range Expansion Into Large River Ecosystems Of The Chesapeake Bay Watershed, Colleen Beth Higgins
Theses and Dissertations
The blue catfish, Ictalurus furcatus (Ictaluridae), is ranked among the most invasive, nonnative species of concern in the Chesapeake Bay watershed. This species, intentionally introduced to three major tributaries and a number of impoundments between 1974 and 1989 for sport fishing, has spread into three additional tributaries. Using samples from the introduced tributary populations as a baseline, we evaluated microsatellite genetic variation in light of demographic and ecological data to elucidate the potential sources of the invasive I. furcatus populations. In general, the populations surveyed in the Chesapeake Bay watershed were considerably more inbred (F ranged from 0.03 - 0.27) …
Phylogeography Of Marine Meiofaunal Nemerteans Of The Ototyphlonemertes Fila Species Complex, Alexander Tulchinsky
Phylogeography Of Marine Meiofaunal Nemerteans Of The Ototyphlonemertes Fila Species Complex, Alexander Tulchinsky
Theses and Dissertations
Morphological conservatism combined with intraspecific variability has obstructed studies of speciation and species boundaries among marine meiofauna. Ototyphlonemertes is a genus of meiofaunal nemerteans inhabiting the interstitial spaces of marine sediments. Its members lack pelagic larvae and dispersal potential is believed to be poor. A phylogeographic study of Ototyphlonemertes fila is presented using mitochondrial (cox3) and nuclear (ISSR) molecular markers. Deep genetic divergence (approximately 18% in cox3) was observed between sympatric mitochondrial lineages in Florida. This divergence was reflected in the nuclear marker as well, suggesting the presence of two cryptic species. The first contains Florida and New England populations …
Anatomical Bases For Auditory Projections To Suprasylvian Visual Areas In The Cat Cerebral Cortex, Giriaj K. Sharma
Anatomical Bases For Auditory Projections To Suprasylvian Visual Areas In The Cat Cerebral Cortex, Giriaj K. Sharma
Theses and Dissertations
How the neural representations of different sensory modalities transition from one to another is an unexplored issue of cortical organization. The present experiments addressed this problem by examining auditory projections to the lateral suprasylvian visual area (LSS) of the cat using neuroanatomical tract tracing methods. Injections of tracer (Biotinylated Dextran Amine, BDA) were made into defined areas of the cat auditory cortex. Following transport and tissue processing, a light microscope with a digitizing stage was used to visualize and plot labeled auditory projections to the LSS. The results showed that all auditory cortices tested demonstrated projections to a restricted portion …
Inhibition Of Margination And Diapedesis Of Neutrophils By Protein Synthesis Blockade, Phyllis V. Acquah
Inhibition Of Margination And Diapedesis Of Neutrophils By Protein Synthesis Blockade, Phyllis V. Acquah
Theses and Dissertations
Acute Respiratory Distress Syndrome (ARDS), an age-old clinical problem facing the Emergency Department and Intensive Care Units of all health systems, is a common debilitating lung condition consequent upon severe systemic inflammation. Although several studies have gone into understanding the epidemiology and pathogenesis of the disease thus making way for new advances in treatment strategies, there seems to be no known study tailored to its prevention. Neutrophil extravasation within the tissues during inflammation is the hallmark of this syndrome. Our study sought to block excessive neutrophil infiltration by inhibiting the biosynthesis of some essential proteins necessary for the process. In …
Degenerate Oligonucleotide Primed-Pcr: Thermalcycling Modifications And Comparison Studies, Denise N. Rodier
Degenerate Oligonucleotide Primed-Pcr: Thermalcycling Modifications And Comparison Studies, Denise N. Rodier
Theses and Dissertations
Degenerate Oligonucleotide Primed-PCR (DOP-PCR) can potentially enhance analysis of low copy number DNA samples. Theoretically, this procedure replicates fragments of the genome that can then be used for downstream multiplex STR analysis. The objective of this study is to optimize DOP-PCR by examining ramplelongation times and cycle numbers in the non-specific amplification portion of DOP-PCR, and by modifying the degenerate primer. Additionally, other methods such as Multiple Displacement Amplification (MDA) and Low Copy Number PCR (LCN PCR) were examined for their ability to create accurate DNA profiles from low DNA input amounts. Increasing the ramplelongation times showed no effect on …
Transforming Growth Factor-Β1 (Tgf-Β1) Induces Mast Cell Apoptosis, Farnaz Norozian
Transforming Growth Factor-Β1 (Tgf-Β1) Induces Mast Cell Apoptosis, Farnaz Norozian
Theses and Dissertations
Mast cells are potent effectors of the inflammatory response, playing an important role in atopy, bacterial immunity, and animal models of arthritis, multiple sclerosis, and heart disease. Hence controlling mast cell numbers and responsiveness is essential for preventing inflammatory disease. This work demonstrated that the cytokine TGF-β1 is a potent inducer of mast cell apoptosis, a finding that was consistent for cultured mouse bone marrow-derived mast cells, peritoneal mast cells, and human mast cells. Cell death appeared to be the result of TGF-mediated repression of IL-3 receptor expression and function, leading to mitochondria1 damage and activation of an apoptotic cascade …
Comparison Of Leukocyte Classes Most Likely To Cause Vascular Dysfunction In Preeclampsia, Kristen Anne Cadden
Comparison Of Leukocyte Classes Most Likely To Cause Vascular Dysfunction In Preeclampsia, Kristen Anne Cadden
Theses and Dissertations
Leukocytes are activated in women with preeclampsia, but the class of leukocyte most likely to cause vascular dysfunction is not known. We hypothesized that neutrophils may be the class of leukocyte most involved in causing this dysfunction because neutrophils are the most abundant of the leukocytes and their numbers increase during pregnancy. In this study we compared vascular infiltration of neutrophils (CD66b) with monocytes/macrophages (CD14) and lymphocytes (CD99) in normal non-pregnant women (NNP), normal pregnant women (NP), and preeclamptic women (PE). There was no significant difference in the infiltration of lymphocytes into the maternal vasculature in PE as compared to …