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Atp Luminescence Assay As A Bioburden Estimator Of Biomass Accumulation In Caves, Janez Mulec, Andreea Oarga-Mulec
Atp Luminescence Assay As A Bioburden Estimator Of Biomass Accumulation In Caves, Janez Mulec, Andreea Oarga-Mulec
International Journal of Speleology
A commercially available adenosine triphosphate (ATP) detection system (Hygiena, USA), supported by cultivable microbial indicators, was used to estimate bioburden in different habitats in and outside show caves: air, water and solid surfaces. A strong positive correlation between ATP concentration expressed as Relative Light Units (RLU) and Colony-Forming-Units (CFU) was observed for swab samples from cave surfaces. In terms of ATP units, surfaces in a single cave system (Postojna Cave) varied considerably (240-1,258,800 RLU/ 20 cm2) and commonly exceeded the bioburden level of analogues on the surface (0-114,390 RLU/ 20 cm2). Cave sub-habitats were colonized by …
Fungi Isolated From Niedźwiedzia Cave In Kletno (Lower Silesia, Poland), Rafał Ogórek, Agnieszka Lejman, Krzysztof Matkowski
Fungi Isolated From Niedźwiedzia Cave In Kletno (Lower Silesia, Poland), Rafał Ogórek, Agnieszka Lejman, Krzysztof Matkowski
International Journal of Speleology
Niedźwiedzia Cave is the most beautiful cave in Poland, discovered in a block of Cambrian marbles. It is the most important part of the reserve established in 1977. The cave is located within the Kłodzko Valley, in Śnieżnik Mt. Massif, in Kleśnica Stream Valley and was discovered in 1966 while working in a quarry. The study aimed at first mycological evaluation of the air and the rocks in Niedźwiedzia Cave. Nine species of filamentous fungi and a yeast species were isolated from the air sampled in the cave, whereas from the rocks - nine species of filamentous fungi and two …