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Genetic And Structural Analyses Of Cytoplasmic Filaments Of Wild-Type Treponema Phagedenis And A Flagellar Filament-Deficient Mutant, Jacques Izard, William A. Samsonoff, Mary Beth Kinoshita, Ronald J. Limberger

Department of Food Science and Technology: Faculty Publications

Unique cytoplasmic filaments are found in the treponeme genus of spirochete bacteria. Their function is unknown, but their location underneath the periplasmic flagellar filaments (PFF) suggests a role in motility and/or cell structure. To better understand these unique structures, the gene coding for the cytoplasmic filaments, cfpA, was identified in various treponemal species. Treponema phagedenis cfpA was 2,037 nucleotides long, and the encoded polypeptide showed 78 to 100% amino acid sequence identity with the partial sequence of CfpA from T. denticola, T. vincentii, and T. pallidum subsp. pertenue. Wild-type T. phagedenis and a PFF-deficient isolate were analyzed by electron microscopy …

Deliberate Introductions Of Species: Research Needs, John J. Ewel, Dennis J. O'Dowd, Joy Bergelson, Curtis C. Daehler, Carla M. D'Antonio, Luis Diego Gómez, Doria R. Gordon, Richard J. Hobbs, Alan Holt, Keith R. Hopper, Colin E. Hughes, Marcy Lahart, Roger R. B. Leakey, William G. Lee, Lloyd L. Loope, David H. Lorence, Svata M. Louda, Ariel E. Lugo, Peter B. Mcevoy, David M. Richardson, Peter M. Vitousek

Svata M. Louda Publications

Research questions about introductions

Several research questions need to be answered to help ensure that proposed introductions are done wisely and safely.

Guarding against risks without sacrificing benefits:
How can the potential benefits and costs of introductions best be evaluated in economic, environmental, and social terms?
Should all introductions be regulated?
How different must organisms or recipient ecosystems be from those assessed previously to warrant independent assessment?
When is it appropriate to assess and regulate taxa other than species?
What are appropriate ecological and political boundaries for regulation?

Alternatives to introductions:
How and when can indigenous organisms be domesticated so …

Insertional Inactivation Of Treponema Denticola Tap1 Results In A Nonmotile Mutant With Elongated Flagellar Hooks, Ronald J. Limberger, Linda L. Slivienski, Jacques Izard, William A. Samsonoff

Department of Food Science and Technology: Faculty Publications

The treponemal fla operon is comprised of numerous motility-related genes; however, the initial gene of this operon, tap1, has no known function. A recently developed system to generate specific mutants in Treponema denticola was utilized to determine if Tap1 was essential for motility. T. denticola tap1 and flanking DNA were identified, cloned, and sequenced, and a suicide plasmid that contained tap1 interrupted with an erythromycin resistance cassette (ermF and ermAM) was constructed. Because of potential polar effects from this cassette, a second plasmid that contained tap1 interrupted with a modified erythromycin resistance cassette that lacked the putative ermF transcription terminator …

Cynopterus Sphinx, Jay F. Storz, Thomas H. Kunz

Jay F. Storz Publications

Order Chiroptera, Family Pteropodidae, Subfamily Pteropodinae, Tribe Cynopterini, Subtribe Cynopterina, Genus Cynopterus. Five species are recognized: C. brachyotis, C. horsfieldi, C. nusatenggara, C. sphinx, and C. titthaecheileus (Koopman, 1993). A key to the species is given in Lekagul and McNeely (1977).

Ec99-828 Retailing Patterns & Trends Across Nebraska, Bruce B. Johnson, Brandon G.Y. Raddatz

University of Nebraska-Lincoln Extension: Historical Materials

This report presents the retailing patterns across Nebraska over time. It assesses historical trends and current conditions. It hopes to present community and business leaders accurate and detailed information to:

a. understand the general trends underway,

b. perform relevant comparative analysis with other communities, and

c. identify strategies which may contribute to retail vitality in their areas.

Nf99-406 Health Care Costs And Financially Troubled Times, Mary Ellen Rider

Agricultural Research Division: News and Annual Reports

This NebFact discusses issues brought up regarding health care in a challenging financial climate.

Tertiary Bats (Mammalia: Chiroptera) From Northern Nebraska, Nicholas J. Czaplewski, Bruce E. Bailey, Richard G. Corner

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Scarce remains of bats are reported from five localities in northern Nebraska in which other kinds of vertebrates are much more common. ?Oligomyotis or ?Myotis, possibly of an undescribed species, is represented by fragments of jaws and humeri from an early Arikareean (late Oligocene) locality in Dawes County. Several toothless jaw fragments from the late Hemingfordian (middle Miocene) Companion Quarry in Sioux County represent an indeterminate microchiropteran. An indeterminate species of Myotis was encountered in the middle Clarendonian (late Miocene) Ashfall site in Antelope County. A hairy-tailed bat, Lasiurus sp. indet., occurred in late Clarendonian (late Miocene) …

Transactions Of The Nebraska Academy Of Sciences Table Of Contents: Volume 25 (1999)

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Editorial Contents

Nebraska Academy of Sciences Officers, Policy Committee ................. ii

Editorial Board ................. iv

Membership Objectives and Friends of the Academy ................. v

Nebraska Association of Teachers of Science ................. vi

Preparation of manuscripts ................. inside back cover

Papers

Using omissive faults to obtain local convergence in partially connected networks (M. H. Azadmanesh and A. W. Krings) ................. 1

New floristic records for Nebraska-5 (Steven B. Rolfsmeier, Robert F. Steinauer, and David M. Sutherland) ................. 15

A key to dicotyledonous rosettes of eastern Nebraska (Phillip D. Moore) ................. 23

The brine shrimp Artemia franciscana in closed microalgal-based microcosms (biospheres) …