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Application Of Ion-Pair Complexation Reaction For The Spectrophotometric Determination Of Clarithromycin In Pharmaceutical Formulations, Sameer A. M. Abdulrahman
Application Of Ion-Pair Complexation Reaction For The Spectrophotometric Determination Of Clarithromycin In Pharmaceutical Formulations, Sameer A. M. Abdulrahman
The Thai Journal of Pharmaceutical Sciences
Two simple, rapid, sensitive, selective and extraction-free spectrophotometric methods have been developed for the determination of clarithromycin (CLR) both in pure form and in pharmaceutical formulations. The developed methods are based on ion-pair com.
Gold Nanoparticle-Colocasia Gigantean Conjugates For Enhancing Cytotoxic Effect Against A375 Melanoma Cell Line, Amornpun Sereemaspun
Gold Nanoparticle-Colocasia Gigantean Conjugates For Enhancing Cytotoxic Effect Against A375 Melanoma Cell Line, Amornpun Sereemaspun
The Thai Journal of Pharmaceutical Sciences
Herbs are a prototype of traditional medicine for primary health care. Their knowledge is the basis of many modern medicinal discoveries. With the new invention of nanomaterials for biomedical applications, gold nanoparticles (AuNPs) have been proven to .
Chemical Compositions And Repellent Activity Of Eucalyptus Tereticornis And Eucalyptus Deglupta Essential Oils Against Culex Quinquefasciatus Mosquito, Rini Pujiarti, Rini Pujiarti
Chemical Compositions And Repellent Activity Of Eucalyptus Tereticornis And Eucalyptus Deglupta Essential Oils Against Culex Quinquefasciatus Mosquito, Rini Pujiarti, Rini Pujiarti
The Thai Journal of Pharmaceutical Sciences
Eucalyptus oils have distinctive smell that not preferably by mosquito and able to repellent mosquito. This research was evaluated chemical compositions and repellent activity of Eucalyptus tereticornis and Eucalyptus deglupta oils against C. quinquefasc.
Effects Of Short-Term Growth Hormone Administration On Plasma Leptin Levels In Normal And Diet-Induced Obesity Rats, Sutharinee Likitnukul
Effects Of Short-Term Growth Hormone Administration On Plasma Leptin Levels In Normal And Diet-Induced Obesity Rats, Sutharinee Likitnukul
Chulalongkorn University Theses and Dissertations (Chula ETD)
Obesity has related to the alteration of hormonal profiles, i.e. growth hormone (GH), insulin and leptin. Because of the pleiotropic mechanisms of GH, it plays important roles both in the pathophysiological mechanisms and the therapeutic hormonal supplementation, which probably affected plasma leptin. The present research aimed firstly to demonstrate the effect of short-term GH administration on plasma leptin in 3 conditions; basal, meal-induced and fasting condition, from the rats with different adipose tissue mass. Secondly, to investigate short-term effect of GH on insulin sensitivity and body adiposity in control, diet resistant (DR) and diet-induced obesity (DIO) rats. In this regard, …
Docking, Synthesis, And Cytotoxic Activity Of N-4-Methoxybenzoyl-N’-(4-Fluorophenyl)Thiourea On Hela Cell Line, Anindi Lupita Nasyanka
Docking, Synthesis, And Cytotoxic Activity Of N-4-Methoxybenzoyl-N’-(4-Fluorophenyl)Thiourea On Hela Cell Line, Anindi Lupita Nasyanka
The Thai Journal of Pharmaceutical Sciences
Tiourea derivatives such as phenylthiourea, benzoyltiourea, tenovin, and many more have been reported as anticancer agent. In this study, we have done some initial step (docking, synthesis, and cytotoxic assay) to develop one of thiourea derivatives, N-4.
Extra Precision Docking Studies Of Novel Luteolin Analogues For The Inhibition Of Tankyrase Ii- “A Theoretical Based Approach Towards Novel Cancer Target, Aravinda Pai, Jayashree B.S., Sanchari Basu-Mallik, Richard Lobo
Extra Precision Docking Studies Of Novel Luteolin Analogues For The Inhibition Of Tankyrase Ii- “A Theoretical Based Approach Towards Novel Cancer Target, Aravinda Pai, Jayashree B.S., Sanchari Basu-Mallik, Richard Lobo
The Thai Journal of Pharmaceutical Sciences
Luteolin, a natural bio-flavonoid is known to possess variety of pharmacological activity, particularly tumour growth inhibition. Reports are available to substantiate the interaction of luteolin with Tankyrase II, one of the key enzymes responsible for .
Oxidative Responses In Transgenic 'Kdml105' Rice Oryza Sativa L. Overexpressing Oscam1-1 Against Drought Stress, Pun Sangchai
Oxidative Responses In Transgenic 'Kdml105' Rice Oryza Sativa L. Overexpressing Oscam1-1 Against Drought Stress, Pun Sangchai
Chulalongkorn University Theses and Dissertations (Chula ETD)
The OsCaM1-1 is a rice calmodulin protein which transduces Ca2+ signals to their target protein that is important to regulate cellular and physiological responses. Drought stress is the one of essential environmental stresses that affected the crop by changing in morphological, physiological, and biochemical direction tactics of plants. In this study, the effect of drought stress on growth and antioxidant system in the transgenic rice overexpressing OsCaM1-1 gene was investigated and compared to the control and wild type KDML 105. The overexpression of OsCaM1-1 in transgenic rice could increase plant tolerance to drought stress, the results showed the greater increase …
Effects Of Processing Methods On Quality Of Green Coffee, Fareeya Kulapichitr
Effects Of Processing Methods On Quality Of Green Coffee, Fareeya Kulapichitr
Chulalongkorn University Theses and Dissertations (Chula ETD)
To study the impact of processing on quality of Arabica green coffee, the research was divided into two parts: I) the study of the effects of postharvest processing practices on the quality of coffee, and II) the study of the effects of heat pump drying conditions on physicochemical properties of coffee with focus on the determination of the key volatile compounds affected by drying treatments. In part 1, the subtopics of the study were: 1.1) the study of the effects of the selection of coffee cherries differing in maturity (ripe vs unripe) on the quality of green coffee based on …
การคัดกรองและการประยุกต์เครื่องหมายไมโครแซทเทลไลท์เพื่อการวิเคราะห์ความหลากหลายทางพันธุกรรมของนกกระเรียนพันธุ์ไทย Grus Antigone Sharpii Blanford, 1895 ในสภาพกรงเลี้ยง, รังสินี สันคม
Chulalongkorn University Theses and Dissertations (Chula ETD)
Grus antigone sharpii จัดเป็นนกกระเรียนที่เคยสูญพันธุ์ไปจากธรรมชาติของประเทศไทย การเพาะเลี้ยงและขยายพันธุ์นกกระเรียนในกรงเลี้ยงเป็นขั้นตอนสำคัญที่สามารถเพิ่มจำนวนประชากรเพื่อนำไปปล่อยคืนสู่ธรรมชาติในอนาคตได้ โดยสิ่งสำคัญที่ต้องคำนึงถึงคือความหลากหลายทางพันธุกรรม โดยเฉพาะอย่างยิ่งในกลุ่มของพ่อแม่พันธุ์ตั้งต้น ในการศึกษาครั้งนี้มีวัตถุประสงค์เพื่อคัดกรองเครื่องหมายไมโครแซทเทลไลท์จำนวน 28 ตำแหน่ง ซึ่งเป็นไพรเมอร์ที่เคยใช้ศึกษาในนกกระเรียน G. americana และนกกระเรียน Anthropoides paradisea เพื่อใช้วิเคราะห์ความหลากหลายทางพันธุกรรมของนกกระเรียนพันธุ์ไทยจากสวนสัตว์เปิดเขาเขียว (KKOZ, n = 11) และสถานีวิจัยเพาะเลี้ยงนกน้ำบางพระ (BB, n = 17) จังหวัดชลบุรี ผลการศึกษาพบว่าไพรเมอร์ทั้ง 28 คู่สามารถเพิ่มปริมาณไมโครแซทเทลไลท์ดีเอ็นเอของนกกระเรียนพันธุ์ไทยได้ แต่มีเพียง 22 คู่ที่แสดงความเป็น polymorphism และมีตำแหน่งไมโครแซทเทลไลท์ที่ปรากฏค่า linkage disequilibrium 8 ตำแหน่ง การเบี่ยงเบนออกจากสมดุล Hardy-Weinberg พบที่โลคัส Gram8 และ Gpa38 ซึ่งเป็นผลมาจาก null allele และการเพิ่มขึ้นของอัลลีลแบบ heterozygous ตามลำดับ จากการวิเคราะห์ค่าความหลากหลายทางพันธุกรรมพบว่า ค่าเฉลี่ยของ expected และ observed heterozygosity มีค่าเท่ากับ 0.63 และ 0.69 ค่า inbreeding coefficient มีค่าเท่ากับ -0.100 ซึ่งแสดงว่านกกระเรียนมีความหลากหลายทางพันธุกรรมค่อนข้างสูง และเกิด inbreeding น้อย นอกจากนี้ยังพบว่านกกระเรียนจาก KKOZ และ BB มีความแตกต่างทางพันธุกรรมต่ำ (FST = 0.035) และไม่มีการแยกออกเป็นกลุ่มประชากรย่อย (K = 1) จากผลการศึกษาครั้งนี้บ่งบอกได้ว่านกกระเรียนพันธุ์ไทยจาก 2 พื้นที่การศึกษาน่าจะเหมาะสมที่จะใช้เป็นพ่อแม่พันธุ์เพื่อเพิ่มจำนวนประชากรในกรงเลี้ยงให้มากขึ้น และสามารถนำไปปล่อยคืนสู่พื้นที่ชุ่มน้ำตามธรรมชาติในอนาคตได้
Bioactive Compounds From Songkhla Province Mangrove-Derived Endophytic Fungi And From Heart-Leaved Moonseed Tinospora Baenzigeri Forman, Sujitra Hanthanong
Bioactive Compounds From Songkhla Province Mangrove-Derived Endophytic Fungi And From Heart-Leaved Moonseed Tinospora Baenzigeri Forman, Sujitra Hanthanong
Chulalongkorn University Theses and Dissertations (Chula ETD)
This study has evaluated biological activities of isolated compounds from Tinospora baenzigeri stems collecting from two different areas, and from mangrove-derived endophytic fungi isolated from trees inhabiting in Songkhla province. Four new rearranged clerodane-type diterpenes (66-69), one new glycoside (70), and six known compounds (61, 71-75), were extracted from plant obtained from local market in Bangkok. Twelve compounds including a new rearranged clerodane-type diterpene (76), a new rearranged clerodane glycoside (77), five known compounds (44, 78-81), and five compounds counting 61, 66, 70 and 74-75 were obtained from plant collected in Bueng Kan Province. Structures of new compounds 66-69 and …
Effects Of The Standardized Extract Of Centella Asiatica Eca233 On Hepatic Phase Ii Drug-Metabolizing Enzymes In Rats, Pitchayapa Seeka, Nuansri Niwattisaiwong, Mayuree H. Tantisira, Songpol Chevapat, Kridsada Anuntawuttikul, Khemchat Apipalakul, Somsong Lawanprasert
Effects Of The Standardized Extract Of Centella Asiatica Eca233 On Hepatic Phase Ii Drug-Metabolizing Enzymes In Rats, Pitchayapa Seeka, Nuansri Niwattisaiwong, Mayuree H. Tantisira, Songpol Chevapat, Kridsada Anuntawuttikul, Khemchat Apipalakul, Somsong Lawanprasert
The Thai Journal of Pharmaceutical Sciences
The standardized extract of C. asiatica ECa233 (ECa 233) was shown to possess neuroprotective and wound healing effects. The purpose of this study was to investigate effects ECa 233 on the activities of phase II drug metabolizing enzymes including UDP-gl.
In Vitro Antimicrobial Activity Of Antidesma Bunius Extracts On Oral Pathogenic Bacteria, Panicha Pongnaratorn, Prakasit Kuacharan, Vayura Kotsuno, Naruwat Pakdee, Pranee Sriraj, Jintana Sattayasai
In Vitro Antimicrobial Activity Of Antidesma Bunius Extracts On Oral Pathogenic Bacteria, Panicha Pongnaratorn, Prakasit Kuacharan, Vayura Kotsuno, Naruwat Pakdee, Pranee Sriraj, Jintana Sattayasai
The Thai Journal of Pharmaceutical Sciences
High antioxidant content of Antidesma bunius may lead to many beneficial pharmaceutical properties such as an anti-bacterial, anti-inflammatory and somatic cell decelerations, as well as cancer prevention. This study aimed at quantifying total phenolic c.
Effects And Mechanisms Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Type 1 And Type 2 On The Permeability And Viability Of Porcine Endometrial Epithelial Cells, Dran Rukarcheep
Chulalongkorn University Theses and Dissertations (Chula ETD)
Both PRRSV types 1 and 2 revealed the sign of reproductive disorders associated with a lesion at implantation sites. Impairment of maternal glandular endometrium cell integrity and function by PRRSV infection may impact a proper nourishing fetus. This research was aimed to examine the effects of PRRSV type 1 and type 2 directly on the viability and barrier function of the endometrium using porcine glandular endometrial epithelial cell culture (PEG). The comparison of the route and the strain of PRRSV infection coinciding with their virulent in reproductive epithelia were considered. PEG cells isolated from the 4-6 months old PRRSV-free-herd gilts …
Potentials Of Ivabradine To Improve Cardiac Function In Dogs With Naturally Occurring, Asymptomatic Degenerative Mitral Valve Disease, Prapawadee Pirintr
Potentials Of Ivabradine To Improve Cardiac Function In Dogs With Naturally Occurring, Asymptomatic Degenerative Mitral Valve Disease, Prapawadee Pirintr
Chulalongkorn University Theses and Dissertations (Chula ETD)
The main hypothesis of the present study is that ivabradine (1.0 mg/kg, orally, twice daily) can reduce heart rate (HR), myocardial oxygen consumption (MVO2) and improve cardiac function in dogs with degenerative mitral valve disease (DMVD) partly due to a reduction of cardiomyocyte apoptosis. In order to test the hypothesis, this study was divided into three parts. The first part aimed to determine the appropriate single oral dose of ivabradine for reduction of HR and MVO2 as assessed by rate-pressure product (RPP= HR x systolic blood pressure). Once the appropriate dose was achieved, the second part was conducted to investigate …
Effects And Mechanisms Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Type 1 And Type 2 On The Permeability And Viability Of Porcine Endometrial Epithelial Cells, Dran Rukarcheep
Chulalongkorn University Theses and Dissertations (Chula ETD)
Both PRRSV types 1 and 2 revealed the sign of reproductive disorders associated with a lesion at implantation sites. Impairment of maternal glandular endometrium cell integrity and function by PRRSV infection may impact a proper nourishing fetus. This research was aimed to examine the effects of PRRSV type 1 and type 2 directly on the viability and barrier function of the endometrium using porcine glandular endometrial epithelial cell culture (PEG). The comparison of the route and the strain of PRRSV infection coinciding with their virulent in reproductive epithelia were considered. PEG cells isolated from the 4-6 months old PRRSV-free-herd gilts …
Potentials Of Ivabradine To Improve Cardiac Function In Dogs With Naturally Occurring, Asymptomatic Degenerative Mitral Valve Disease, Prapawadee Pirintr
Potentials Of Ivabradine To Improve Cardiac Function In Dogs With Naturally Occurring, Asymptomatic Degenerative Mitral Valve Disease, Prapawadee Pirintr
Chulalongkorn University Theses and Dissertations (Chula ETD)
The main hypothesis of the present study is that ivabradine (1.0 mg/kg, orally, twice daily) can reduce heart rate (HR), myocardial oxygen consumption (MVO2) and improve cardiac function in dogs with degenerative mitral valve disease (DMVD) partly due to a reduction of cardiomyocyte apoptosis. In order to test the hypothesis, this study was divided into three parts. The first part aimed to determine the appropriate single oral dose of ivabradine for reduction of HR and MVO2 as assessed by rate-pressure product (RPP= HR x systolic blood pressure). Once the appropriate dose was achieved, the second part was conducted to investigate …
Cloning And Characterisation Of Alternansucrase From Leuconostoc Citreum Abk-1 And Role Of Surface Aromatic Acids On Product Size, Karan Wangpaiboon
Cloning And Characterisation Of Alternansucrase From Leuconostoc Citreum Abk-1 And Role Of Surface Aromatic Acids On Product Size, Karan Wangpaiboon
Chulalongkorn University Theses and Dissertations (Chula ETD)
The alternansucrase (ALT, EC 2.4.1.140) catalyses transferring of glucose from sucrose to produce a polymer with α-1,6 and α- 1,3 linkages, so-called "alternan". The ALT-encoding gene from Leuconostoc citreum ABK-1 (WTalt) was successfully cloned and expressed in Escherichia coli BL21 (DE3). The two versions of truncated SH3-like domains, ∆3SHALT and ∆7SHALT, were successfully constructed. The WTALT, ∆3SHALT and ∆7SHALT possessed optimum temperature of 40 °C, while optimum pH of WTALT was at 5.0 but ∆3SHALT's and ∆7SHALT's were shifted to pH 4.0. Their activities could be enhanced by Mn²⁺. Kinetic studies of all ALTs were mainly responsible for transglycosylation activity …
Expression And Characterization Of Α-Glucosidase From Weissella Confusa Bbk-1, Lalita Silapasom
Expression And Characterization Of Α-Glucosidase From Weissella Confusa Bbk-1, Lalita Silapasom
Chulalongkorn University Theses and Dissertations (Chula ETD)
Alpha-glucosidase [EC 3.2.1.20; α-D-glucoside glucohydrolase] is an exohydrolase which catalyzes non-reducing end of substrates to release D-glucose. In addition, alpha-glucosidase also displays a transferase activity, which bring about the formation of α-glucosylated compounds such as soluble starch and glycogen. This research aims to express and characterize α-glucosidase from Weissella confusa BBK-1 (WcAG). WcAG gene was expressed in modified pET28b vector. This gene contained an open reading frame of 1,775 bps. Optimum expression condition of WcAG was cultured in LB medium containing 1% (w/v) glucose and induced with 0.4 mM IPTG at 20°C for 20 h. The recombinant WcAG containing his-tag …
Cloning And Characterization Of Bacterial Mannanase Isolated From Dynastes Hercules Larvae, Sitipon Leerawatthanakun
Cloning And Characterization Of Bacterial Mannanase Isolated From Dynastes Hercules Larvae, Sitipon Leerawatthanakun
Chulalongkorn University Theses and Dissertations (Chula ETD)
Bacterial isolate HM7 was isolated from Dynastes hercules larvae excrement. It was identified as Bacillus sp. by 16S rRNA gene analysis. When the bacteria were cultured for β-mannanase production in medium containing konjac flour it had the highest specific activity of 138 U/mg at 36 h, and when cultured in medium containing coconut meal it had the highest specific of 114 U/mg at 48 h. The 1089 base pairs long of a β-mannanase encoding gene (Man26HM7) was successfully cloned and expressed in Escherichia coli BL21 (DE3), using pET21-b expression system. The enzyme was purified and characterized. The optimal condition MAN26HM7catalysis …
Isolation And Characterization Of Triclocarban Degrading Bacteria As Plant Growth Promoter, Merry Krisdawati Sipahutar
Isolation And Characterization Of Triclocarban Degrading Bacteria As Plant Growth Promoter, Merry Krisdawati Sipahutar
Chulalongkorn University Theses and Dissertations (Chula ETD)
Triclocarban (TCC) is a widely used as antimicrobial agent in household products. High usage volume of TCC results in its accumulation in sludge and lead to contamination in environments. Application of biosolid in agricultural activities leads to contamination of TCC in agricultural areas. So. development of bioremediation technique for TCC is necessary to maintain quality of agricultural ecosystem. For that purpose, this research isolated 5 soil bacteria capable of TCC degradation and with plant-growth promoting (PGP) activities including Ochrobactrum sp. MC22, Ochrobactrum sp. MC35, Sphingobacterium sp. MC43, Pseudomonas sp. MS45, and Pseudomonas fluorescens MC46. Among them, Ochrobactrum sp. MC22 and …
Intracellular Trafficking Of Different Shaped Oxidized Carbon Nanoparticles, Banphot Jiangchareon
Intracellular Trafficking Of Different Shaped Oxidized Carbon Nanoparticles, Banphot Jiangchareon
Chulalongkorn University Theses and Dissertations (Chula ETD)
Oxidized carbon nanospheres (OCSs) and graphene oxide sheets (GOShs) were prepared by oxidation of graphite. Oxidized carbon nanotubes (OCTs) was synthesized from single-walled carbon nanotubes. All three shapes of oxidized carbon nanoparticles (OCNs) were used for investigation of their intracellular trafficking in human embryonic kidney cells (HEK293T), mouse macrophage cells (RAW264.7) and human liver cancer cells (HepG2). The results revealed all three shapes of OCNs were internalized into through HEK293T and RAW264.7 cells without endosomal trapping, including localization of all the three OCNs in HEK293T's lysosome. In case of RAW264.7 cells, OCTs and GOShs were localized in lysosomes, only OCSs …
Screening Of Spore Forming D-Lactic Acid Producing Bacteria And Its Optimization, Budsabathip Prasirtsak
Screening Of Spore Forming D-Lactic Acid Producing Bacteria And Its Optimization, Budsabathip Prasirtsak
Chulalongkorn University Theses and Dissertations (Chula ETD)
In this study, isolation, screening, fermentation optimization, and determination of the expression of key enzymes involving in D-lactic acid production were conducted for selection of the novel D-lactic acid isolate with the high yield, productivity, and optical purity. From the screening experiment, the novel isolate, NK26-11T, was obtained. The isolate NK26-11T was a Gram-stain-positive, catalase-positive, facultatively anaerobic, spore-forming, rod-shaped bacterium isolated from soil sample in Thailand. This isolate homofermentatively fermented glucose for D-lactic acid and grew at the wide range of temperature between 20 and 45 °C and the pH of 5-8.5. The cell-wall peptidoglycan of NK26-11T contained meso-diaminopimelic acid. …
Development Of Molecular Technique For Risk Assessment And Risk Management Of Listeria Monocytogenes And L. Innocua In Frozen Cooked Chicken Plant, Chirapiphat Phraephaisarn
Development Of Molecular Technique For Risk Assessment And Risk Management Of Listeria Monocytogenes And L. Innocua In Frozen Cooked Chicken Plant, Chirapiphat Phraephaisarn
Chulalongkorn University Theses and Dissertations (Chula ETD)
Thailand is one of the largest cooked chicken meat exporting country in the world. Unfortunately, these products can be contaminated with L. monocytogenes causes the disease Listeriosis which carries a fatality rate of 30 to 40 percent. Therefore, the various importing countries including Japan and the European countries require a zero tolerance for L. monocytogenes in the cooked chicken products. Besides, all Listeria spp. occurrence, and not only L. monocytogenes, in these products is also unacceptable to the importers. Rejection of Listeria contaminated product can reach over 5 to 20 million baht per factory per year. Hence, the aim of …
Development Of Immunoassay On Paper-Based Microfluidic Devices For Determination Of Ractopamine, Alpha-Fetoprotein, Human Thyroid Stimulating Hormone, Pattarachaya Preechakasedkit
Development Of Immunoassay On Paper-Based Microfluidic Devices For Determination Of Ractopamine, Alpha-Fetoprotein, Human Thyroid Stimulating Hormone, Pattarachaya Preechakasedkit
Chulalongkorn University Theses and Dissertations (Chula ETD)
In this dissertation, immunoassays on paper-based microfluidic devices have been developed using different types of label for various applications in agriculture safety and medical diagnosis, which can be divided into three parts. In the first part, the conjugation method of ractopamine (RAC) and protein carrier is developed for fabricating paper-based lateral flow strip test of RAC using gold nanoparticles (AuNPs) as a label. The developed conjugation method provided a simple and one-step method with the use of less chemicals. The strip test for RAC determination in animal feed offers a linearity of 0.075-0.750 ng g-1 and a limit of detection …
Bioactive Peptide Derived From Trypsin Hydrolysis Of Unicellular Cyanobacterium Synechococcus Sp., Rutairat Suttisuwan
Bioactive Peptide Derived From Trypsin Hydrolysis Of Unicellular Cyanobacterium Synechococcus Sp., Rutairat Suttisuwan
Chulalongkorn University Theses and Dissertations (Chula ETD)
A free radicals can promote denaturing reactions in many cellular components, which create oxidative stress and it results to damage in body tissue. The free radicals can causing many diseases including heart diseases, Alzheimer, inflammation and cancer. This research aimed to investigate the effect of bioactive peptides derived from Synechococcus sp. cultured for 21 days on antioxidant, anti-inflammation and anticancer activities. The Synechococcus sp. protein hydrolysate was prepared by trypsin digest and purified by ultrafiltration with molecular weight cut off membranes of 10, 5 and 3 kDa. The MW <3 kDa fraction showed the highest NO, ABTS and DPPH radical scavenging activities with IC50 values of 34.51±9.8 µg protein/mL, IC50 11.54±0.3 µg protein/mL and IC50 13.63±0.15 µg protein/mL, respectively. The F2 fraction from gel filtration chromatography showed the strongest NO and ABTS radical scavenging activities with IC50 values of 7.68±0.64 µg protein/mL and 9.74±0.2 µg protein/mL, respectively. The F2 was purified by RP-HPLC to yield four fractions. The 30 – 40 min sub-fraction (F2-4) was selected for further analysis by mass spectroscopy. Five isolated peptides with amino acid sequences of AILQSYSAGKTK; 1,265.69 Da, ALNKTHLIQTK; 1,265.74 Da, LLVHAPVK; 875.55 Da, IPDAHPVK; 875.48 Da and VVVLRDGAVQQLGTPR; 1,706.97 Da were identified. The F2-4 had higher DPPH and NO radical scavenging activity compared to the synthetic peptide. Moreover, AILQSYSAGKTK had the highest ABTS radical scavenging activity. Furthermore, the F2 fraction protected oxidation-induced DNA damage in pBR322, pKS and pUC19 cells. F2 fraction was selected to study anti-inflammatory and anticancer properties. Anti-inflammatory effect, F2 fraction showed no cytotoxicity toward RAW264.7 macrophage cells. RT-PCR and qRT-PCR results showed that F2 reduced gene expression of pro-inflammatory cytokines iNOS, TNF-α, COX-1, COX-2 and IL-6. For anticancer activity, F2 fraction showed high anticancer activities in the human colon cancer cells (SW620) according to cytotoxic activity (MTT assay), with IC50 values of 106.58±21.46 µg protein/mL. The F2 fraction activated the apoptotic pathway in SW620 cells after treatment for 24, 48 and 72 hours. The highest activities of caspases 3, 8 and 9 were observed after treatment for 72 hours. These findings suggested that unicellular cyanobacterium Synechococcus sp. may be used to develop for natural anti-inflammation and natural anticancer drugs.
Specific Binding Of Hlas And Antigenic Peptides Associated With Behcet's Disease And Systemic Sclerosis, Sirilak Kongkaew
Specific Binding Of Hlas And Antigenic Peptides Associated With Behcet's Disease And Systemic Sclerosis, Sirilak Kongkaew
Chulalongkorn University Theses and Dissertations (Chula ETD)
Human leukocyte antigen (HLA) molecules are essential components for immune functions. There play a major role to present antigenic-peptide for T-cell receptor (TCR) recognition and immune activation. Association between HLA genes and infectious as well as autoimmune diseases are identified genetic risk factors. For instance, Behçet’s disease (BD) and systemic sclerosis (SSc) are autoimmunity result in chronic of multisystemic inflammation and visceral fibrosis, respectively. To investigate the link of HLAs correlated with self-peptides for BD and SSc, molecular dynamics (MD) simulations were applied on the HLAp complexes. For BD, a self-peptide named MICA-TM was modeled for two BD-association such as …
Adaptation Of Terrilactibacillus Laevilacticus Sk5-6 For Growth And D-Lactic Acid Production In A Low Yeast Extract Concentration Medium, Srettapat Limsampancharoen
Adaptation Of Terrilactibacillus Laevilacticus Sk5-6 For Growth And D-Lactic Acid Production In A Low Yeast Extract Concentration Medium, Srettapat Limsampancharoen
Chulalongkorn University Theses and Dissertations (Chula ETD)
Lactic acid has been extensively used in food, personal care, and pharmaceutical products. Besides, lactic acid can be used as the sole feedstock in the synthesis of polylactic acid (PLA), the biodegradable plastic. The demand of the bioplastics including PLA has been increased due to the environmental impact of petroleum based plastic; therefore, this expedited the market demand of lactic acid. It should be noted that the polymer grade lactic acid is unlike those commercially available products for food and pharmaceutical applications. The high purity of stereoisomer either D-lactate or L-lactate is mandatory in PLA polymerization. In addition, the production …
Uv-A And -B Blocking Agents For Psoriasis Phototherapy, Sruangsuda Mawaha
Uv-A And -B Blocking Agents For Psoriasis Phototherapy, Sruangsuda Mawaha
Chulalongkorn University Theses and Dissertations (Chula ETD)
UV-A and -B blocking agents are essential components for the formulation of topical cream for psoriasis phototherapy. In this research, many natural compounds were discovered as potent UV-A and UV-B blocking agents, but small number could absorb narrowband UV-B. Quercetin hydrate, oxyresveratrol and curcumin were good UV-A blocking agents, while catechin, hesperetin, lutein and naringenin were proper for UV-B blocking agents. Berberine chloride hydrate, demethoxycurcumin and mansonone G were UV-A and -B blocking agents. From random combination, the mixture of mansonone G 0.010 % w/v and quercetin hydrate 0.0025 % w/v was disclosed to be a right choice for this …
Bioactive Compounds With An Inhibitory Activity Against Osteoclast Differentiation And Mechanisms, Supatta Chawalitpong
Bioactive Compounds With An Inhibitory Activity Against Osteoclast Differentiation And Mechanisms, Supatta Chawalitpong
Chulalongkorn University Theses and Dissertations (Chula ETD)
โรคกระดูกพรุนเป็นปัญหาสุขภาพที่สำคัญอย่างหนึ่งของสังคมผู้สูงอายุ สาเหตุหลักของภาวะดังกล่าวเกิดจากความไม่สมดุลของเซลล์ที่ทำหน้าทีสร้างกระดูก (osteoblast) และเซลล์ที่ทำหน้าที่สลายกระดูก (osteoclast) การลดลงของมวลกระดูกในผู้สูงอายุนำมาซึ่งความเสี่ยงในการแตกหักของกระดูก จุดประสงค์ของการศึกษาครั้งนี้ คือ การคัดกรองสารออกฤทธิ์ทางชีวภาพที่มีฤทธิ์ในการต้านการเปลี่ยนสภาพของเซลล์สลายกระดูกและกลไกการออกฤทธิ์ของสารนั้นๆ ในกลุ่มสารทดสอบอนุพันธ์สาร (3S)-1-(3,4-dihydroxyphenyl)-3-hydroxy-7-phenyl-(6E)-6-heptene (DHPH) และ กรดไซเปอเรโนอิก (Cyperenoic acid) ซึ่งสกัดได้จากส่วนเหง้า Curcuma comosa Rox. และราก Croton crassifolius Geiseler. ตามลำดับ มีฤทธิ์แรงในการต้านการเกิดเซลล์สลายกระดูก ในระดับ in vitro ใช้เซลล์จากไขกระดูกที่ถูกกระตุ้นด้วย receptor activator of nuclear factor-κB ligand (RANKL) DHPH มีฤทธิ์ในการยับยั้งการเปลี่ยนสภาพไปเป็นเซลล์สลายกระดูก โดยยับยั้งการเกิด TRAP-positive multinucleated cells (TRAP+ MNCs) ที่ระดับความเข้มข้นยับยั้งกึ่งหนึ่ง (IC50) 325±1.37 นาโนโมลาร์ การยับยั้งดังกล่าวสอดคล้องกับการลดต่ำลงของสัญญาณภายใต้การกระตุ้นของ RANKL โดยรวมถึงวิถี Mitogen-activated protein kinases (MAPKs) เช่น ERK (p44/42) และ p38 แต่ไม่มีผลต่อวิถี NF-κB โดยผลของสารดังกล่าวยังส่งผลกระทบต่อระดับของ transcription factor เช่น nuclear factor of activation T cells (NFATc1) และ c-Fos ซึ่งมีความสำคัญมากในการเปลี่ยนสภาพเป็นเซลล์สลายกระดูก DHPH ลดการกัดกร่อนกระดูก (bone resorption) บนแผ่นกระดูก (bone slices) นอกจากนั้นสาร DHPH ยังสามารถเพิ่มกิจกรรมของเอนไซม์ alkaline phosphatase (ALP) ที่เกี่ยวข้องกับการเปลี่ยนสภาพของเซลล์สร้างกระดูกในเซลล์ชนิด MC3T3-E1 ในกรณีของกรดไซเปอเรโนอิกนั้น พบว่าสามารถลดการเกิด TRAP+ MNCs ที่ระดับความเข้มข้น IC50 ที่ 36.69±1.02 …
Increasing Viability Of Probiotic Bacteria Co-Encapsulated With Prebiotic For Disease Resistance In White Shrimp Litopenaeus Vannamei, Suppharada Jantarathin
Increasing Viability Of Probiotic Bacteria Co-Encapsulated With Prebiotic For Disease Resistance In White Shrimp Litopenaeus Vannamei, Suppharada Jantarathin
Chulalongkorn University Theses and Dissertations (Chula ETD)
The main problem of probiotics is the low survival of these microorganisms in food products and sensitive to harsh conditions during food processing, storage and in the gastrointestinal tract. The suitable technology for protecting probiotics to maintain the high number of probiotic cells in product and survived within the host which is microencapsulation technique. The aim of this work was to investigate the effect of prebiotic which is inulin or Jerusalem artichoke on the survival of encapsulated Lactobacillus acidophilus TISTR 1338 within alginate matrix and double-coated with chitosan after freeze-drying process and heat processing at 70°C for 60 min. Furthermore, …