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Molecular Cloning Of Lin-29, A Heterochronic Gene Required For The Differentiation Of Hypodermal Cells And The Cessation Of Molting In C.Elegans, A. Papp, A. Rougvie, Victor Ambros

Victor R. Ambros

The lin-29 gene product of C.elegans activates a temporal developmental switch for hypodermal cells. Loss-of-function lin-29 mutations result in worms that fail to execute a stage-specific pattern of hypodermal differentiation that includes exist from the cell cycle, repression of larval cuticle genes, activation of adult cuticle genes, and the cessation of molting. Combined genetic and physical mapping of restriction fragment length polymorphisms (RFLPs) was used to identify the lin-29 locus. A probe from the insertion site of a Tc1 (maP1), closely linked and to the left of lin-29 on the genetic map, was used to identify a large set of …