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Molecular Mechanisms Of Bacillus Thuringiensis Resistance In The Sugarcane Borer, Yunlong Yang Jan 2011

Molecular Mechanisms Of Bacillus Thuringiensis Resistance In The Sugarcane Borer, Yunlong Yang

LSU Doctoral Dissertations

The sugarcane borer, Diatraea saccharalis, is a major target pest of transgenic corn expressing Bacillus thuringiensis (Bt) proteins in many areas of the U.S. mid-southern region. A Cry1Ab-resistant (Cry1Ab-RR) strain of D. saccharalis has been developed from a single two-parent family-line. To examine the molecular mechanisms of the Cry1Ab resistance in this insect strain, cDNAs of five types of potential candidate genes related to Bt resistance were sequenced using reverse transcriptase polymerase chain reaction (RT-PCR) and 5’ rapid amplification of cDNA end (5’ RACE). The Bt resistance candidate genes examined included three trypsins (DsTRYs), three chymotrypsins (DsCHYs), three aminopeptidases N …


Ecology And Systematics Of Coleoptera In Woody Debris Of Eastern North American Forests, Michael Leslie Ferro Jan 2011

Ecology And Systematics Of Coleoptera In Woody Debris Of Eastern North American Forests, Michael Leslie Ferro

LSU Doctoral Dissertations

Dead wood is a largely unexplored reservoir of taxonomic diversity and its ecology is poorly known despite its use as fuel and its roles in the carbon cycle and healthy ecosystems. During this research 15 new species of dead wood associated rove beetles (Staphylinidae: Pselaphinae) in the genus Sonoma Casey were discovered and described. Sonoma tolulae (LeConte) was redescribed. Taxonomic issues obfuscated the identity of another dead wood associate, Thoracophorus costalis (Erichson) (Staphylinidae: Osoriinae). Examination of holotypes resulted in the discovery of two nomenclatural synonyms: T. longicollis Motschulsky, and T. fletcheri Wendeler. An inexpensive emergence chamber used to collect insects …


Advanced Management Of The Mexican Rice Borer (Eoreuma Loftini) In Sugarcane, Blake Emerson Wilson Jan 2011

Advanced Management Of The Mexican Rice Borer (Eoreuma Loftini) In Sugarcane, Blake Emerson Wilson

LSU Master's Theses

Insecticide, greenhouse and varietal resistance experiments were conducted in Texas to develop management tactics for the Mexican rice borer, Eoreuma loftini (Dyar), sugarcane. A 3-treatment, large plot aerial application study was set up in 5 commercial sugarcane fields (35-84 acres) to evaluate the utilization of pheromone traps to improve chemical control strategies for E. loftini during 2009 and 2010 growing seasons. A threshold of 20-25 moths/trap/wk was used as an indicator to initiate monitoring for E. loftini larval infestations. Larval infestations were directly related to the no. of moths/trap/wk (R2=0.71). Reductions in borer injury and adult emergence (60% and 40% …


Revision And Reclassification Of The Genera Of Phalacridae (Coleoptera: Cucujoidea), Matthew Gimmel Jan 2011

Revision And Reclassification Of The Genera Of Phalacridae (Coleoptera: Cucujoidea), Matthew Gimmel

LSU Doctoral Dissertations

The family Phalacridae is monographed at the genus level using modern phylogenetic and classification methods. A morphological matrix of 106 characters and 2063 base pairs of aligned 18S rDNA sequence data were analyzed both separately and in a concatenated matrix, using maximum parsimony, Bayesian methods, and maximum likelihood. The phylogenetic hypotheses were used to construct a new subfamilial classification for the family. I recognize eight subfamilies, including two described as new: Biophytinae Guillebeau 1894, Eustilbinae Guillebeau 1892, Ochrolitinae Guillebeau 1894, Olibrinae Guillebeau 1892, Olibroporinae, subfam. nov., Olibrosominae, subfam. nov., Phaenocephalinae Matthews 1899, Phalacrinae Guillebeau 1892. Twenty-eight new generic synonymies are …


Role Of Substrate Moisture, Relative Humidity And Temperature On Survival And Foraging Behavior Of Formosan Subterranean Termites, Bal Krishna Gautam Jan 2011

Role Of Substrate Moisture, Relative Humidity And Temperature On Survival And Foraging Behavior Of Formosan Subterranean Termites, Bal Krishna Gautam

LSU Doctoral Dissertations

The Formosan subterranean termite is an important structural pest in the southern United States, especially Louisiana. Baiting systems and spot treatment with non-repellent delayed action termiticides are among the most eco-friendly control approaches. However, to achieve a desired success from these approaches requires a sophisticated knowledge of foraging behavior and food transfer system of these social insects. Moisture and temperature play a vital role in influencing the foraging behavior of these desiccation prone insects. A series of laboratory experiments were conducted to understand the subtleties of foraging behavior of Coptotermes formosanus Shiraki in relation to moisture, relative humidity and temperature. …


A Study Of Horn Fly, Haematobia Irritans (L.) (Diptera: Muscidae), Target-Site Sensitivity, Susceptibility, And Resistance Management At Selected Sites In Louisiana, Cole David Younger Jan 2011

A Study Of Horn Fly, Haematobia Irritans (L.) (Diptera: Muscidae), Target-Site Sensitivity, Susceptibility, And Resistance Management At Selected Sites In Louisiana, Cole David Younger

LSU Doctoral Dissertations

The purpose of the first study was to evaluate the effectiveness of the use of a mid-season avermectin pour-on to cattle for managing OP resistance in horn fly populations at the Rosepine Louisiana Agricultural Research Station. During an eight year study, ivermectin treatments were made three times (when the treatment threshold of less than five weeks of control was reached), and in the following years, the number of weeks of control increased to 9 to 10 weeks each time. The purpose of the second study was to test a two-year OP/one-year pyrethroid rotation strategy as a strategy for maintaining susceptibility …


Ecology And Chemical Ecology Of Plant-Insect Interactions In Rice: Implications For Pest Management, Jason Charles Hamm Jan 2011

Ecology And Chemical Ecology Of Plant-Insect Interactions In Rice: Implications For Pest Management, Jason Charles Hamm

LSU Doctoral Dissertations

Induced resistance to the rice water weevil, Lissorhoptrus oryzophilus, was assessed in greenhouse and field experiments. The fall armyworm,Spodoptera frugiperda, and an elicitor, jasmonic acid, were used to induce resistance. The effect of these treatments on rice resistance to oviposition varied between cultivar used, but significantly fewer larvae were found on plants exposed to S. frugiperda and jasmonic acid on both cultivars tested. Application of jasmonic acid significantly reduced the number of L. oryzophilus larvae per plant, and represents the first example of elicitor-induced resistance in rice in field experiments. Oviposition by the sugarcane borer, Diatraea saccharalis, induced resistance to …


Molecular Mechanisms Of Bacillus Thuringiensis Resistance In The Sugarcane Borer, Yunlong Yang Jan 2011

Molecular Mechanisms Of Bacillus Thuringiensis Resistance In The Sugarcane Borer, Yunlong Yang

LSU Doctoral Dissertations

The sugarcane borer, Diatraea saccharalis, is a major target pest of transgenic corn expressing Bacillus thuringiensis (Bt) proteins in many areas of the U.S. mid-southern region. A Cry1Ab-resistant (Cry1Ab-RR) strain of D. saccharalis has been developed from a single two-parent family-line. To examine the molecular mechanisms of the Cry1Ab resistance in this insect strain, cDNAs of five types of potential candidate genes related to Bt resistance were sequenced using reverse transcriptase polymerase chain reaction (RT-PCR) and 5’ rapid amplification of cDNA end (5’ RACE). The Bt resistance candidate genes examined included three trypsins (DsTRYs), three chymotrypsins (DsCHYs), three aminopeptidases N …


Introduction And Establishment Of Cyrtobagous Salviniae Calder And Sands (Coleoptera: Curculionidae) For The Control Of Salvinia Minima Baker (Salviniaceae), And Interspecies Interactions Possibly Limiting Successful Control In Louisiana, Katherine A. Parys Jan 2011

Introduction And Establishment Of Cyrtobagous Salviniae Calder And Sands (Coleoptera: Curculionidae) For The Control Of Salvinia Minima Baker (Salviniaceae), And Interspecies Interactions Possibly Limiting Successful Control In Louisiana, Katherine A. Parys

LSU Doctoral Dissertations

Common salvinia, Salvinia minima Baker, is a floating aquatic invasive macrophyte that obstructs waterways and causes problems in wetlands across Louisiana and Texas. The salvinia weevil, Cyrtobagous salviniae Calder and Sands, has been released in over 14 countries around the world for the biological control of Salvinia spp. We successfully monitored the introduction and establishment of C. salviniae on S. minima in southern Louisiana between 2006 and 2010. Cyrtobagous salviniae significantly lowered the biomass of S. minima and increased the number of terminal buds damaged but had no significant impact on pH, dissolved oxygen, conductivity, surface temperature, percent of surface …


Agroecological Factors Impacting Stem Borer (Lepidoptera: Crambidae) Dynamics In Gulf Coast Sugarcane And Rice, Julien M. Beuzelin Jan 2011

Agroecological Factors Impacting Stem Borer (Lepidoptera: Crambidae) Dynamics In Gulf Coast Sugarcane And Rice, Julien M. Beuzelin

LSU Doctoral Dissertations

Diatraea saccharalis (F.) and Eoreuma loftini (Dyar) are stem boring pests of sugarcane (Saccharum spp.) and rice (Oryza sativa L.) crops in the Gulf Coast region. Studies were conducted to determine the role of agroecological factors, including predator disruptions, alternate hosts, and crop phenological conditions, on stem borer populations.
The year after Hurricane Rita storm surge flooded sugarcane in Louisiana, a 71% reduction in the predaceous Solenopsis invicta Buren was recorded. Even with a 2.4-fold increase in the number of insecticide applications used for D. saccharalis management in flooded fields, growers still incurred higher injury.
In two field …


Redbanded Stink Bug, Piezodorus Guildinii (Westwood): Pest Status, Control Strategies, And Management In Louisiana Soybean, Joshua H. Temple Jan 2011

Redbanded Stink Bug, Piezodorus Guildinii (Westwood): Pest Status, Control Strategies, And Management In Louisiana Soybean, Joshua H. Temple

LSU Doctoral Dissertations

The redbanded stink bug, Piezodorus guildinii (Westwood), was identified as a pest of Louisiana soybean during 2000. Currently, this species has become established throughout Louisiana and into the soybean production regions of bordering states. Seasonal abundance, patterns of oviposition, and population dynamics of this species were surveyed in soybean during 2008-10. The redbanded stink bug comprised the largest percentage (54%) of the total stink bug complex collected at five survey sites followed by the southern green (27%), Nezara viridula L. and brown (7%) stink bugs, Euschistus servus L. Peak oviposition occurred during the (R5) soybean growth stage. Most (80%) egg …


Contribution Of Bacillus Thuringiensis Cotton Cultivars And Insecticides To A Fall Armyworm, Spodoptera Frugiperda (J. E. Smith), (Lepidoptera : Noctuidae) Management Strategy, Jarrod T. Hardke Jan 2011

Contribution Of Bacillus Thuringiensis Cotton Cultivars And Insecticides To A Fall Armyworm, Spodoptera Frugiperda (J. E. Smith), (Lepidoptera : Noctuidae) Management Strategy, Jarrod T. Hardke

LSU Doctoral Dissertations

Fall armyworm, Spodoptera frugiperda (J. E. Smith), response to cottons, Gossypium hirsutum L., expressing Bacillus thuringiensis (Bt) insecticidal proteins, Cry1Ac (Bollgard®), Cry1Ac + Cry2Ab (Bollgard II®), and Cry1Ac + Cry1F (WideStrike™), was evaluated in field and laboratory experiments. In field trials, larvae that were infested on selected fruiting forms (squares, white flowers, and bolls) of WideStrike™ plants had lower survivorship and caused less injury than larvae on non-Bt plants, regardless of fruiting structures. Bollgard® and Bollgard II® plants produced no consistent negative effects on fall armyworm survivorship and injury. In no-choice laboratory assays, Bollgard II® and WideStrike™ cotton tissue reduced …


Characterization Of The Subcortical Interactions Between Larvae Of The Southern Pine Sawyer Monochamus Titillator (F.) And The Larvae Of The Southern Pine Beetle Guild Using Molecular Gut Analyses, Erich N. Schoeller Jan 2011

Characterization Of The Subcortical Interactions Between Larvae Of The Southern Pine Sawyer Monochamus Titillator (F.) And The Larvae Of The Southern Pine Beetle Guild Using Molecular Gut Analyses, Erich N. Schoeller

LSU Master's Theses

The southern pine beetle guild (Coleoptera: Curculionidae: Scolytinae) is arguably the most destructive group of forest pests in the Southeastern United States. Laboratory assays suggest that larvae of wood borer associates from the genus Monochamus (Coleoptera: Cerambycidae) may be facultative intraguild predators of southern pine beetle guild. In this study the dynamics of the subcortical interactions between M. titillator (F.) and members of the southern pine beetle guild were examined using PCR-based molecular gut content analyses. Species-specific PCR primer sets were developed to work under multiplex PCR conditions to detect DNA of members of southern pine beetle guild in the …