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Influence Of Four Veterinary Antibiotics On Constructed Treatment Wetland Nitrogen Transformation, Matthew V. Russell, Tiffany L. Messer, Deborah A. Repert, Richard L. Smith, Shannon L. Bartelt-Hunt, Daniel D. Snow, Ariel P. Reed

Department of Civil and Environmental Engineering: Faculty Publications

The use of wetlands as a treatment approach for nitrogen in runoff is a common practice in agroecosystems. However, nitrate is not the sole constituent present in agricultural runoff and other biologically active contaminants have the potential to affect nitrate removal efficiency. In this study, the impacts of the combined effects of four common veterinary antibiotics (chlortetracycline, sulfamethazine, lincomycin, monensin) on nitrate-N treatment efficiency in saturated sediments and wetlands were evaluated in a coupled microcosm/mesocosm scale experiment. Veterinary antibiotics were hypothesized to significantly impact nitrogen speciation (e.g., nitrate and ammonium) and nitrogen uptake and transformation processes (e.g., plant uptake and …

Nanopesticide Influence On Nitrogen Cycling In Soils, Jacob Richardson

Theses and Dissertations--Biosystems and Agricultural Engineering

Nanotechnology has several applications in the agricultural industry, as the small size of nanoparticles and high reactivity enables targeted delivery of pesticides and fertilizers to the intended crop. Copper-based nanopesticides such as Kocide, are proposed as a more efficient method for targeted pest control. Despite the potential benefits of nanopesticides, little is known regarding the transport and implications of nanopesticides under long-term inundation conditions often found in downstream wetlands. Specifically, the impact of nanopesticides on microbial nitrogen processes in wetland environments remains unknown. Therefore, this thesis explores the impact of nanopesticides, specifically Kocide, on microbial nitrogen cycles in agricultural and …

Fate Of Metals In Presence Of Minerals And Mineral-Organic Assemblages, Neha Sharma

McKelvey School of Engineering Theses & Dissertations

Metals can enter aquatic systems from natural and anthropogenic processes associated with weathering, sediment re-suspension, industrial activities, and atmospheric deposition. Metals pose health and environmental risks at high concentrations due to their potential toxicity and bioaccumulation, but many trace metals also serve as essential micronutrients for biogeochemical processes in natural aquatic systems. Biogeochemical processes such as methanogenesis, denitrification, and mercury methylation require transition metals such as nickel (Ni), cobalt (Co), copper (Cu), and molybdenum (Mo) for completion. These biogeochemical processes can be substantial contributors of greenhouse gases, such as methane (CH4) and nitrous oxide (N2O), into the atmosphere. The behavior, …

Nitrate Removal During The Shoulder Months In The Occoquan Reservoir: A Comprehensive Study, Km Risaduzzaman

Electronic Theses and Dissertations

The artificial nitrate addition by the Upper Occoquan Service Authority (UOSA)’s Water Reclamation Facility (WRF) to the Occoquan Reservoir during the warmer months has been proven to be an efficient way of maintaining the water quality of the reservoir. During the warmer months, the water body became thermally stratified, resulting in hypolimnetic anoxia and the subsequent release of undesired matters from the reduced sediments. At the end of the warmer months, the temperature began to decrease, and by the beginning of winter, oxygen became ubiquitous in water because of fall turnover. This condition remained stable until the following thermal stratification; …

Combining High-Frequency Sensor Data And Nutrient Addition Experiments To Identify Uptake Dynamics In Streams, Justin Rae Nichols

Civil Engineering ETDs

Nutrient enrichment has led to impaired waterways across the globe with an estimated 90% of waterways in the U.S. being negatively impacted from anthropogenic sources. In the past 30 years, there has been significant focus on studying nutrient uptake within fluvial systems. However, there are still critical knowledge gaps about the controls of nutrient dynamics and how these processes scale. This study combined the results from traditional instantaneous nutrient addition experiments and sensor data averaged over ±5 days from the time of addition to determine mechanisms that affect nutrient dynamics in two contrasting streams within the same watershed, over almost …

Evaluating The Impact Of External Carbon Source In Laboratory-Based Denitrification Rate Experiments, Jared Richens

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Limitations in the quality of transformation rates used with water quality models such as QUAL2K have led to the need to identify methods that can effectively and accurately determine these rates. During the early stages of method development of a Utah Department of Environmental Quality (Utah DEQ) funded project seeking to identify such methods, it was determined that under certain situations external carbon amendment may be required to successfully determine denitrification rates.

The focus of this study was to evaluate the potential impacts that introducing external carbon sources may have on estimating the laboratory denitrification rates resulting from these methods. …

Microbial Competition In Bioelectrochemical Systems, Varun Srinivasan

Doctoral Dissertations

Bioelectrochemical systems(BESs)/ microbial fuel fells (MFCs) are a well-studied potential technology for bioremediation and decentralized wastewater treatment. However, progress has been somewhat stalled at the bench-scale. In well controlled experiments electron recovery is high. In natural environments, wastewaters are complex and anode-respiring bacteria can be outcompeted in the presence of competing microorganisms, leading to a loss in electron-recovery and power production. Furthermore, the cathode of the MFC plays a vital role in providing flexibility for treatment options but is an understudied part of MFCs. Modelling Intracellular Competition in a Denitrifying Biocathode: One potential MFC configuration uses an organic-oxidizing anode biofilm …

Regulation-Structured Dynamic Metabolic Model Provides A Potential Mechanism For Delayed Enzyme Response In Denitrification Process, Hyun-Seob Song, Dennis G. Thomas, James C. Stegen, Minjing Li, Chongxuan Liu, Xuehang Song, Xingyuan Chen, Jim K. Fredrickson, John M. Zachara, Timothy D. Scheibe

Department of Biological Systems Engineering: Papers and Publications

In a recent study of denitrification dynamics in hyporheic zone sediments, we observed a significant time lag (up to several days) in enzymatic response to the changes in substrate concentration. To explore an underlying mechanism and understand the interactive dynamics between enzymes and nutrients, we developed a trait-based model that associates a community’s traits with functional enzymes, instead of typically used species guilds (or functional guilds). This enzyme-based formulation allows to collectively describe biogeochemical functions of microbial communities without directly parameterizing the dynamics of species guilds, therefore being scalable to complex communities. As a key component of modeling, we accounted …

The Role Of Nitrification And Denitrification In Successful Cultivation Of Oxygenic Photogranules For Wastewater Treatment, Kristie Stauch-White

Environmental & Water Resources Engineering Masters Projects

Oxygenic photogranules (OPGs) are compact, spherical, self-immobilizing biofilms in the form of dense aggregates of microorganisms with a predomination of filamentous cyanobacteria cultivated from photoilluminated activated sludge. In this study, aerobic and anaerobic nitrogen transformation processes occurring simultaneously within OPGs, during both successful and unsuccessful cultivations, were examined. Chemical analyses including ammonium, nitrate, and nitrite concentrations at the beginning and periodically during OPG cultivations indicated nitrogen transformations occurring during successful cultivations. Additionally, qPCR studies revealed that successful cultivations supported a greater relative abundance of cyanobacteria, nitrifying, and denitrifying populations during the cultivation period. Dissolved oxygen microprofiles within a successfully cultivated …

The Effect Of The Antecedent Dry Conditions On Nitrogen Removal For A Modified Bioretention System, Mackenzie Peterson

USF Tampa Graduate Theses and Dissertations

Eutrophication is defined as the ‘over enrichment’ of a water body from nutrients, resulting in uncontrolled growth of primary producers, leading to periods of oxygen depletion from decomposition of the algal organic matter. According to the 2010 Water Infrastructure Needs and Investment (a U.S. Congressional Report), 40% of U.S. water bodies are contaminated with pollutants, including nutrients. Non-point sources of nutrient pollution are a major cause of this reduction in water quality. One way to decrease eutrophication is to manage nutrients found in stormwater runoff, before they reach a receiving water body.

Bioretention cells containing an internal water storage zone …

Resource Recovery Through Halophyte Production In Marine Aquaponics: An Evaluation Of The Nutrient Cycling And The Environmental Sustainability Of Aquaponics, Suzanne Boxman

USF Tampa Graduate Theses and Dissertations

Aquaculture, the farming of aquatic animals and plants, is an important component of global food production, which supplies a nutritious protein source for millions of people. Interest in improving the sustainability of aquaculture has led to the development of aquaponics in which fish production is combined with plant production to create zero-discharge systems. A need for more fundamental science and engineering research on marine aquaculture and growing interest in production of halophytes motivated this novel research on marine aquaponics. One objective was to evaluate the growth and nutrient removal capacity of halophytes in marine aquaponics. Bench-scale studies were conducted to …

Nitrogen Removal In Biological Wastewater Treatment Plant, Courtney Greyn

Graduate Theses & Non-Theses

Wastewater treatment and disposal is an integral part of mining operations. Water must be carefully managed to prevent waterborne contaminants from entering the surrounding environment. Mining companies rely on regulatory compliance and social acceptance to continue operations. The Stillwater Mining Company operates two underground mines in southern Montana, the Stillwater Mine and the East Boulder Mine, to extract platinum group metals from the J-M Reef geological formation. The East Boulder Mine operates an on-site wastewater treatment plant to remove waterborne contaminants from the water pumped to the surface from the underground mining operations. The main contaminant of concern at the …

Evaluation And Modeling Of Internal Water Storage Zone Performance In Denitrifying Bioretention Systems, Thomas Joseph Lynn

USF Tampa Graduate Theses and Dissertations

Nitrate (NO3) loadings from stormwater runoff promote eutrophication in surface waters. Low Impact Development (LID) is a type of best management practice aimed at restoring the hydrologic function of watersheds and removing contaminants before they are discharged into ground and surface waters. Also known as rain gardens, a bioretention system is a LID technology that is capable of increasing infliltration, reducing runoff rates and removing pollutants. They can be planted with visually appealing vegetation, which plays a role in nutrient uptake. A modified bioretention system incorporates a submerged internal water storage zone (IWSZ) that includes an electron donor to support …

Biofilm Control In A Fludized Bed Bioreactor (Fbbr) Treating Municipal Wastewater: Optimization, Modeling, And Nitrous Oxide (N2o) Emissions, Ahmed Eldyasti

Electronic Thesis and Dissertation Repository

Among the various biological wastewater treatment processes for industrial and municipal wastewater treatment, fluidized bed bioreactors (FBBR) demonstrate numerous advantages compared to suspended growth systems such as lower hydraulic retention time, high surface area and accordingly high biomass retention time, higher volumetric conversion rates, lower sensitivity to temperature, and less sludge production. Despite the numerous biofilm bioreactor configurations and system schemes that are currently available for a wide variety of environmental applications, the development and optimization of a stable biofilm that is capable of offering effective and integrated functions, i.e. biodegradation, biomass-liquid separation, and biomass retention along with a substantial …

Impact Of Salt On Denitrification Potential In Roadside Environments, Nakita A. Lancaster

Master's Theses

Urban systems are impacted by changes in hydrology and water chemistry, altering their ability to mitigate detrimental impacts to coastal water bodies, particularly those that result from high volumes of stormwater runoff. The elevated level of impervious cover increases not only runoff but also contaminant loading of nutrients, metals, and road salt used for deicing in cold weather climates. Current stormwater management strategies, while focused on managing the changes in hydrology associated with urbanization, also are designed to mitigate the pollutant loading. Here we investigate the impact that road salt will have on nitrogen removal in roadside environments. Sediments were …

Investigation Of A Commercial Product (Biowish™) For Nitrogen Management, Eva Lee

Master's Theses

Abstract

Investigation of a Commercial Product (BiOWiSH­­^TM) for Nitrogen Management

Eva Lee

BiOWiSH–Aqua, which has the capability of treating nitrogen from wastewater through bioaugmentation, is a commercial product consisting of a blend of microorganisms developed by BiOWiSH Technologies. A study of the treatment of nitrogen compounds (i.e. , , and ) using Biowish–Aqua was conducted using small scale experiments (flask experiments) and large scale experiments (column reactor experiments). In this work, column reactors were created to test Biowish–Aqua’s nitrogen treating capabilities by providing enough depth to simulate the dissolved oxygen gradient that can be observed in a pond. …

Biogeochemical Cycling And Nutrient Control Strategies For Groundwater At Stormwater Infiltration Basins, Andrew M. O'Reilly

Electronic Theses and Dissertations

Elevated concentrations of nutrients, particularly nitrate, in groundwater and springs in Florida are a growing resource management concern. Stormwater infiltration basins, which are a common stormwater management practice in the well-drained karst terrain areas of Florida, are a potentially important source of nutrients to the groundwater system because stormwater exits the basin by only evaporation or infiltration. To better understand the biogeochemical processes integrating stormwater infiltration impacts on groundwater resources in a field-scale setting, a combination of hydrologic, soil chemistry, water chemistry, dissolved and soil gas, isotope, and microbiological data was collected from 2007 through 2010 at two stormwater infiltration …

A Swmm-5 Model Of A Denitrifying Bioretention System To Estimate Nitrogen Removal From Stormwater Runoff, Michelle D. Masi

USF Tampa Graduate Theses and Dissertations

This research estimates nitrogen removal from stormwater runoff using a denitrifying bioretention system using the USEPA Storm Water Management Model Version 5 (SWMM-5). SWMM-5 has been used to help planners make better decisions since its development in 1971. A conventional bioretention system is a type of Low Impact Development (LID) technology, which designed without a media layer specifically for achieving nitrogen removal. More recently studies have showed that high TN removal efficiencies are possible when incorporating a denitrification media layer. These systems are known as denitrifying bioretention systems, or alternative bioretention systems. LID projects are currently being designed and developed …

The Effectiveness Of Specifically Designed Filter Media To Reduce Nitrate And Orthophosphate In Stormwater Runoff, Mikhal Moberg

Electronic Theses and Dissertations

Throughout Central Florida surface water and ground water are decreasing in quantity and quality in part because of excess Nitrate and Phosphorus nutrients. Stormwater runoff serves as a medium for transport of Nitrate and Phosphorus to surface water and ground water. The goal of this experiment is assess the Nitrate and Phosphorus removal in stormwater using select media. The results of a literature search, batch test experimentation and column test experimentation are used to determine an optimal media blend that may be implemented in detention ponds to reduce Nitrate and Phosphorus. The extensive literature search revealed 32 different media that …

Fate Of Nitrogen And Phosphorus Species From A Black And Goldtm Nugget Mix In A Laboratoy Column Simulated Septic Tank Drainfiel, Timir Shah

Electronic Theses and Dissertations

The presence of nitrates and phosphorus in ground water is a worldwide problem. A septic tank with drainfield that is conventionally designed does not typically remove nitrogen in the form of nitrates. The main risks are in "Blue baby" syndrome and suspected carcinogenic effect of nitrates on humans and the nutrient enrichment of receiving waters. In some areas nitrate and phosphorus removal are essentially required. Thus the information in this report concentrates on using media in the drainfield for the removal of nitrogen and phosphorus. Extensive work has been conducted in the past few decades in order to find suitable …

Evaluation Of Prefermentation As A Unit Process Upon Biological Nutrient Removal Including Biokinetic And Wastewater Parameters, Terrence Mccue

Electronic Theses and Dissertations

The objective of this dissertation was to provide a controlled comparison of identical continuous flow BNR processes both with and without prefermentation in order to provide a stronger, more quantitative, technical basis for design engineers to evaluate the potential benefits of prefermentation to EBPR in treating domestic wastewater. In addition, the even less understood effect of prefermentation on denitrification kinetics and anoxic phosphorus (P) uptake was studied and quantified. Other aspects of BNR performance, which might change due to use of prefermentation, will also be addressed, including anaerobic stabilization. Potential benefits to BNR processes derived from prefermentation are compared and …

Optimization Of Nitrogen Removal In Sequencing Batch Reactor, Suppakit Poonyachat

Theses

Operating parameters for sequencing batch reactor have the influenceon each substrate concentration. Concentration profile changes as operationparameters are changed. The study was conducted to model the variationin effluent concentration from sequencing batch reactor. MLVSS and cycletime are the parameters that were varied. Concentration in Fill, React,and Settle Period were calculated by using kinetic equations.

The results can show that these parameters have effects on the concentrationprofile. The increase of MLVSS can lower the concentration of BOD andammonium concentration in React period. Increasing MLVSS can show thatmore substrate utilization and nitrification process occur more rapidly.Concentration of nitrate at the end of …