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Detailed Mechanistic And Optimization Of The Photochemical Production Method Of Superoxide, Ta-Chung Ong
Detailed Mechanistic And Optimization Of The Photochemical Production Method Of Superoxide, Ta-Chung Ong
Honors Theses
The highly reactive nature of superoxide anion presents an analytical challenge to prepare stable standards for instrument calibration. McDowell et al. (1983) developed a convenient method of superoxide production based on continuous photolysis of benzophenone and acetone in oxygen saturated, alkaline, 2-propanol solution. In the McDowell reaction, ketones are photolyzed to their triplet state, and then reacted with alcohol to produce ketyl radicals that react with oxygen to produce superoxide. This study investigated the mechanism and rate of these reactions by Laser Flash Photolysis (LFP). Reaction rate constants for the reaction between the benzophenone triplet and ethanol was 5.5 x …
Quantification Of Nanomolar Superoxide In Aqueous Solution : Flow Injection Analysis Using The Chemiluminescent Reagent Mcla., Nathan Boland
Quantification Of Nanomolar Superoxide In Aqueous Solution : Flow Injection Analysis Using The Chemiluminescent Reagent Mcla., Nathan Boland
Honors Theses
A flow injection system using the chemiluminescent reagent 2-methyl-6-(4methoxyphenyl)- 3,7-dihydroimidazo[1,2-a]pyrazin-3-one (MCLA) was used to quantify aqueous concentrations of superoxide. Stock solutions of superoxide were produced by photolysis of a solution of 2-propanol and benzophenone. The concentration of superoxide was quantified in the stock solutions by direct measurement of the absorbance at 240 nm. Quantified additions of superoxide to the flow injection analysis system reacted with MCLA at pH 5.1 demonstrated a detection limit of 0.074 nM superoxide. Selective superoxide detection was confirmed by pH-dependent decay of superoxide, which was in excellent agreement with published rates.