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A Determination Of Phylogeny And Hybridization History Within Clematis L. (Ranunculaceae) Using Actin And Nitrate Reductase Intron Sequences, Kimberly Fearn Do
A Determination Of Phylogeny And Hybridization History Within Clematis L. (Ranunculaceae) Using Actin And Nitrate Reductase Intron Sequences, Kimberly Fearn Do
USF Tampa Graduate Theses and Dissertations
The phylogeny of Clematis, section Viorna, was characterized in this study using molecular data. Two nuclear introns were sequenced for a variety of taxa: actin and nitrate reductase. Actin intron sequence data yielded very little phylogenetic information. Some basal clades were resolved, but there were very few well supported relationships between species of the Viorna section in both the neighbor joining and maximum parsimony analyses. Nitrate reductase intron sequence data was slightly more variable. The number of well supported relationships in both the neighbor joining and maximum parsimony analyses for nitrate reductase was greater, but still not sufficient …
Plasmid Probes For Screening Specific Genes In Large Fragment Dna Libraries, Leah Ferguson
Plasmid Probes For Screening Specific Genes In Large Fragment Dna Libraries, Leah Ferguson
Senior Honors Projects
Heliothis virescens, a member of the lepidopteran family, is of great importance to many people who rely on agriculture as a source of income because it is a pest of many important crops. Because H. virescens feeds on a broad array of hosts including cotton, tobacco, tomato, and soybean it has earned its place as a major nuisance in the United States. Heliothis subflexa is a close taxonomic relative of H. virescens, but the two species differ in that H. subflexa is a specialized herbivore that only feeds on plants in the genus Physalis. Studies have been performed to determine …
Analyzing Dna Microarrays With Undergraduate Statisticians, Johanna S. Hardin, Laura Hoopes, Ryan Murphy '06
Analyzing Dna Microarrays With Undergraduate Statisticians, Johanna S. Hardin, Laura Hoopes, Ryan Murphy '06
Pomona Faculty Publications and Research
With advances in technology, biologists have been saddled with high dimensional data that need modern statistical methodology for analysis. DNA microarrays are able to simultaneously measure thousands of genes (and the activity of those genes) in a single sample. Biologists use microarrays to trace connections between pathways or to identify all genes that respond to a signal. The statistical tools we usually teach our undergraduates are inadequate for analyzing thousands of measurements on tens of samples. The project materials include readings on microarrays as well as computer lab activities. The topics covered include image analysis, filtering and normalization techniques, and …
Degenerate Oligonucleotide Primed-Pcr: Thermalcycling Modifications And Comparison Studies, Denise N. Rodier
Degenerate Oligonucleotide Primed-Pcr: Thermalcycling Modifications And Comparison Studies, Denise N. Rodier
Theses and Dissertations
Degenerate Oligonucleotide Primed-PCR (DOP-PCR) can potentially enhance analysis of low copy number DNA samples. Theoretically, this procedure replicates fragments of the genome that can then be used for downstream multiplex STR analysis. The objective of this study is to optimize DOP-PCR by examining ramplelongation times and cycle numbers in the non-specific amplification portion of DOP-PCR, and by modifying the degenerate primer. Additionally, other methods such as Multiple Displacement Amplification (MDA) and Low Copy Number PCR (LCN PCR) were examined for their ability to create accurate DNA profiles from low DNA input amounts. Increasing the ramplelongation times showed no effect on …
Biochemical Responses Of Dimorphic Seeds Of Arthrocnemum Indicum Willd. During Germination, Inhibition, And Alleviation Under Saline And Non-Saline Conditions, Zamin Shaheed Siddiqui
Biochemical Responses Of Dimorphic Seeds Of Arthrocnemum Indicum Willd. During Germination, Inhibition, And Alleviation Under Saline And Non-Saline Conditions, Zamin Shaheed Siddiqui
Turkish Journal of Biology
Dimorphic seeds of Arthrocnemum indicum (a halophytic shrub) exhibit diverse germination responses following salinity treatment and subsequent exposure to growth regulators by adjusting their physiological and biochemical processes. The present study examined the effects of 24-h and 72-h NaCl treatment on germination, germination velocity, and the associated biochemical changes in protein and RNA contents of 2 seed morphs, and compared these effects after transfer into distilled water or 10 mM thiourea solution. Treatment for 24 and 72 h in NaCl solution induced a significant reduction in the rate of germination and final percent germination of both seed morphs. No germination …