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An Automated Method For Rapid Identification Of Putative Gene Family Members In Plants, Ronald L. Frank, Ajay Mane, Fikret Erçal Sep 2006

An Automated Method For Rapid Identification Of Putative Gene Family Members In Plants, Ronald L. Frank, Ajay Mane, Fikret Erçal

Biological Sciences Faculty Research & Creative Works

Background: Gene duplication events have played a significant role in genome evolution, particularly in plants. Exhaustive searches for all members of a known gene family as well as the identification of new gene families has become increasingly important. Subfunctionalization via changes in regulatory sequences following duplication (adaptive selection) appears to be a common mechanism of evolution in plants and can be accompanied by purifying selection on the coding region. Such negative selection can be detected by a bias toward synonymous over nonsynonymous substitutions. However, the process of identifying this bias requires many steps usually employing several different software programs. We …


Comparative Sequence Analysis And Tissue Localization Of Members Of The Slc6 Family Of Transporters In Adult Drosophila Melanogaster, Matthew S. Thimgan, Jonathan S. Berg, Ann E. Stuart Sep 2006

Comparative Sequence Analysis And Tissue Localization Of Members Of The Slc6 Family Of Transporters In Adult Drosophila Melanogaster, Matthew S. Thimgan, Jonathan S. Berg, Ann E. Stuart

Biological Sciences Faculty Research & Creative Works

The SLC6 family comprises proteins that move extracellular neurotransmitters, amino acids and osmolytes across the plasma membrane into the cytosol. In mammals, deletion of SLC6 family members has dramatic physiologic consequences, but in the model organism Drosophila melanogaster, little is known about this family of proteins. Therefore, in this study we carried out an initial analysis of 21 known or putative SLC6 family members from the Drosophila genome. Protein sequences from these genes segregated into either well-defined subfamilies, including the novel insect amino acid transporter subfamily, or into a group of weakly related sequences not affiliated with a recognized …


Microbial Growth And Biofilm Formation In Geologic Media Is Detected With Complex Conductivity Measurements, Caroline A. Davis, Estella A. Atekwana, Eliot A. Atekwana, Lee D. Slater, Silvia Rossbach, Melanie R. Mormile Sep 2006

Microbial Growth And Biofilm Formation In Geologic Media Is Detected With Complex Conductivity Measurements, Caroline A. Davis, Estella A. Atekwana, Eliot A. Atekwana, Lee D. Slater, Silvia Rossbach, Melanie R. Mormile

Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works

Complex conductivity measurements (0.1-1000 Hz) were obtained from biostimulated sand-packed columns to investigate the effect of microbial growth and biofilm formation on the electrical properties of porous media. Microbial growth was verified by direct microbial counts, pH measurements, and environmental scanning electron microscope imaging. Peaks in imaginary (interfacial) conductivity in the biostimulated columns were coincident with peaks in the microbial cell concentrations extracted from sands. However, the real conductivity component showed no discernible relationship to microbial cell concentration. We suggest that the observed dynamic changes in the imaginary conductivity (σ″) arise from the growth and attachment of microbial cells and …


Oxidase-Coupled Amperometric Glucose And Lactate Sensors With Integrated Electrochemical Actuation System, Jongwon Park, Chang-Soo Kim, Minsu Choi Aug 2006

Oxidase-Coupled Amperometric Glucose And Lactate Sensors With Integrated Electrochemical Actuation System, Jongwon Park, Chang-Soo Kim, Minsu Choi

Electrical and Computer Engineering Faculty Research & Creative Works

Unpredictable baseline drift and sensitivity degradation during continuous use are two of the most significant problems of biosensors including the amperometric glucose and lactate sensors. Therefore, the capability of on-demand in situ calibration/diagnosis of biochemical sensors is indispensable for reliable long-term monitoring with minimum attendance. Another limitation of oxidase enzyme-based biosensors is the dependence of enzyme activity on the background oxygen concentration in sample solution. In order to address these issues, the electrolytic generation of oxygen and hydrogen bubbles were utilized 1) to overcome the background oxygen dependence of glucose and lactate sensors and 2) to demonstrate the feasibility of …


2nd Annual Undergraduate Research Conference Abstract Book, University Of Missouri--Rolla Apr 2006

2nd Annual Undergraduate Research Conference Abstract Book, University Of Missouri--Rolla

Undergraduate Research Conference at Missouri S&T

No abstract provided.


Automated Oxidase-Coupled Amperometric Microsensor With Integrated Electrochemical Actuation System For Continuous Sensing Of Saccharoids, Yadunandana Yellambalase, Jongwon Park, Chang-Soo Kim, Minsu Choi, Nohpill Park, Fabrizio Lombardi Apr 2006

Automated Oxidase-Coupled Amperometric Microsensor With Integrated Electrochemical Actuation System For Continuous Sensing Of Saccharoids, Yadunandana Yellambalase, Jongwon Park, Chang-Soo Kim, Minsu Choi, Nohpill Park, Fabrizio Lombardi

Electrical and Computer Engineering Faculty Research & Creative Works

Recent developments for biosensors have been mainly focused on miniaturization and exploratory use of new materials. It should be emphasized that the absence of a novel "in-situ self-calibration/diagnosis technique" that is not connected to an external apparatus is a key obstacle to the realization of a biosensor for continuous use with minimum attendance. To address this deficiency, a novel needle-type biosensor system with fully automated operations is being developed, in which a novel oxidase-coupled amperometric sensor with oxygen depleting/generating actuator is interfaced with an electrochemical instrument and a perfusion system. Labview virtual instrument has been also developed to oversee the …


Disinfection Of Swine Wastewater Using Chlorine, Ultraviolet Light And Ozone, John J. Macauley, Zhimin Qiang, Rao Y. Surampalli, Melanie R. Mormile, Craig D. Adams Jan 2006

Disinfection Of Swine Wastewater Using Chlorine, Ultraviolet Light And Ozone, John J. Macauley, Zhimin Qiang, Rao Y. Surampalli, Melanie R. Mormile, Craig D. Adams

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

Veterinary antibiotics are widely used at concentrated animal feeding operations (CAFOs) to prevent disease and promote growth of livestock. However, the majority of antibiotics are excreted from animals in urine, feces, and manure. Consequently, the lagoons used to store these wastes can act as reservoirs of antibiotics and antibiotic-resistant bacteria. There is currently no regulation or control of these systems to prevent the spread of these bacteria and their genes for antibiotic resistance into other environments. This study was conducted to determine the disinfection potential of chlorine, ultraviolet light and ozone against swine lagoon bacteria. Results indicate that a chlorine …


Design And Implementation Of A Storage Management Method For Content Distribution, Chang-Soo Kim, Yu-Hyeon Bak, Sang-Min Woo, Won-Jae Lee, Nunm-Ok Gee, Hag-Young Kim Jan 2006

Design And Implementation Of A Storage Management Method For Content Distribution, Chang-Soo Kim, Yu-Hyeon Bak, Sang-Min Woo, Won-Jae Lee, Nunm-Ok Gee, Hag-Young Kim

Electrical and Computer Engineering Faculty Research & Creative Works

The SMART system is a special purpose server developed by ETRI and designed for efficient streaming services over high speed networks. The SMART server has one or more special purpose NS (Network-Storage) card. The NS card has several disks that store multimedia contents. However all of the multimedia contents to be serviced cannot be stored at the server. In this paper, we will describe the storage management mechanism in design and implementation aspects. With this storage management mechanism, the SMART server can provide effectiveness in managing storage and distributing some contents from a source station to streaming service servers.