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Positive Regulation Of Pka On Human Gonadotropin-Releasing Hormone (Hgnrh) Gene Expression In Human Placental Jeg-3 Cells, Zhaoyang Wen
Positive Regulation Of Pka On Human Gonadotropin-Releasing Hormone (Hgnrh) Gene Expression In Human Placental Jeg-3 Cells, Zhaoyang Wen
Chemistry & Biochemistry Theses & Dissertations
Using the human placental choriocarcinoma JEG-3 cell line as an in vitro human placental model, we studied the mechanisms of the PKA positive regulation of the hGnRH gene expression in the human placenta. Studies in JEG3 cells showed that through the PKA catalytic subunit a, human GnRH upstream promoter activity was stimulated by PKA signaling pathway in a cAMP dependent mechanism. The sequence between —202 (Afl II) and —554 (BamH I) base pair in the human GnRH upstream promoter region appeared to be responsible for the PKA positive regulation of the gene expression. Furthermore, Western blot analysis demonstrated the involvement …
Cloning And Expression Analysis Of Hemoglobin Genes From Maize (Zea Mays Ssp. Mays) And Teosinte (Zea Mays Ssp. Parviglumis), Elena Aréchaga-Ocampo, Juan Saenz-Rivera, Gautam Sarath, Robert V. Klucas, Raúl Arredondo-Peter
Cloning And Expression Analysis Of Hemoglobin Genes From Maize (Zea Mays Ssp. Mays) And Teosinte (Zea Mays Ssp. Parviglumis), Elena Aréchaga-Ocampo, Juan Saenz-Rivera, Gautam Sarath, Robert V. Klucas, Raúl Arredondo-Peter
Gautam Sarath Publications
With the exception of barley and rice, little is known about the existence of hemoglobins (Hbs) in cereals. This work reports the cloning and analysis of hb genes from maize (Zea mays ssp. mays) and teosinte (Zea mays ssp. parviglumis). Coding sequences of maize and teosinte hb genes (hbm and hbt, respectively) are highly similar to each other and are interrupted by three introns located at identical positions as other plant hb genes. Sequences of predicted Hbm and Hbt proteins are identical. The hydropathic profile of Hbm and Hbt is highly similar to that …