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Biochemistry, Biophysics, and Structural Biology
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Assembly Of The Type Ii Secretion System Such As Found In Vibrio Cholerae Depends On The Novel Pilotin Asps, Rhys A. Dunstan, Eva Heinz, Lakshmi C. Wijeyewickrema, Robert N. Pike, Anthony W. Purcell, Timothy J. Evans, Judyta Praszkier, Roy M. Robins-Browne, Richard A. Strugnell, Konstantin V. Korotkov, Trevor Lithgow
Assembly Of The Type Ii Secretion System Such As Found In Vibrio Cholerae Depends On The Novel Pilotin Asps, Rhys A. Dunstan, Eva Heinz, Lakshmi C. Wijeyewickrema, Robert N. Pike, Anthony W. Purcell, Timothy J. Evans, Judyta Praszkier, Roy M. Robins-Browne, Richard A. Strugnell, Konstantin V. Korotkov, Trevor Lithgow
Molecular and Cellular Biochemistry Faculty Publications
The Type II Secretion System (T2SS) is a molecular machine that drives the secretion of fully-folded protein substrates across the bacterial outer membrane. A key element in the machinery is the secretin: an integral, multimeric outer membrane protein that forms the secretion pore. We show that three distinct forms of T2SSs can be distinguished based on the sequence characteristics of their secretin pores. Detailed comparative analysis of two of these, the Klebsiella-type and Vibrio-type, showed them to be further distinguished by the pilotin that mediates their transport and assembly into the outer membrane. We have determined the crystal structure of …