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Effects Of Lead And Cadmium On Brain Endothelial Cell Survival, Monolayer Permeability, And Crucial Oxidative Stress Markers In An In Vitro Model Of The Blood-Brain Barrier, Shakila Tobwala, Hsiu Jen Wang, Joshua Warren Carey, William A. Banks, Nuran Ercal
Effects Of Lead And Cadmium On Brain Endothelial Cell Survival, Monolayer Permeability, And Crucial Oxidative Stress Markers In An In Vitro Model Of The Blood-Brain Barrier, Shakila Tobwala, Hsiu Jen Wang, Joshua Warren Carey, William A. Banks, Nuran Ercal
Chemistry Faculty Research & Creative Works
Oxidative stress, which is the loss of balance between antioxidant defense and oxidant production in the cells, is implicated in the molecular mechanism of heavy metal-induced neurotoxicity. Given the key role of lead (Pb) and cadmium (Cd) in inducing oxidative stress, we investigated their role in disrupting the integrity and function of immortalized human brain microvascular endothelial cells (hCMEC/D3). To study this, hCMEC/D3 cells were exposed to control media or to media containing different concentrations of Pb or Cd. Those exposed to Pb or Cd showed significantly higher oxidative stress than the untreated group, as indicated by cell viability, reactive …
Characterization Of Vasoactive Intestinal Peptide Receptors On Human Megakaryocytes And Platelets, Sang K. Park, Thomas A. Olson, Nuran Ercal, Monica Summers, M. Sue O'Dorisio
Characterization Of Vasoactive Intestinal Peptide Receptors On Human Megakaryocytes And Platelets, Sang K. Park, Thomas A. Olson, Nuran Ercal, Monica Summers, M. Sue O'Dorisio
Chemistry Faculty Research & Creative Works
Vasoactive intestinal peptide receptor I (VIPRI) expression was examined in megakaryocytes using reverse transcriptase-polymerase chain reaction (RT- PCR). VIPRI protein was characterized in platelet membranes using covalent crosslinking techniques. Human megakaryocytes were isolated from suspension cultures of cord blood and adult bone marrow mononuclear cells using a murine monoclonal antibody to human platelet glycoprotein IIB/IIIA (CD41) and immunomagnetic beads. RT-PCR primers were constructed for the VIP, VIPRI, and VIPRII genes as well as for megakaryocyte specific genes, c-mpl and platelet factor 4 (PF-4). VIP, VIPRI, c-mpl, and PF-4 were coexpressed in megakaryocyte mRNA. Southern blot analysis confirmed the expression of …