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Examination Of Canine Herpesvirus-1 In Dogs By Serological, Molecular, And Pathological Methods, Serkan Yildirim, Fatma Gülten Bayraktar, Hakan Aydin, Yavuz Seli̇m Sağlam, Gi̇zem Eser, Muhammet Bahaeddi̇n Dörtbudak

Turkish Journal of Veterinary & Animal Sciences

In this study, it was aimed to examine Canine Herpesvirus-1 (CHV-1) with different diagnostic methods and investigate the pathological changes in positive samples. For this purpose, 100 dog uteruses of different ages and breeds, were removed by ovariohysterectomy and their blood samples were used as the study material. Enzyme-linked immunosorbent assay (ELISA) and Indirect Fluorescent Antibody Test (IFAT) for serological diagnosis, polymerase chain reaction (PCR) method for molecular diagnosis, immunohistochemistry (IHC) and immunofluorescence (IF) staining for immunopathological diagnosis were performed on the samples. Additionally, hematoxylin and eosin (HE) staining was performed to examine the histopathological changes in the CHV- 1-positive …

Initial Evaluation Of Cyclic Adenosine Monophosphate Enzyme Immunoassay For Use With Crane Semen Samples, Megan E. Brown, Kim Boardman, Katie L. Edwards

Proceedings of the North American Crane Workshop

The management of ex situ and in situ populations of many wildlife species requires detailed knowledge of a species’ reproductive biology. For species such as cranes, where artificial insemination is a critical component of ex situ management strategies, understanding normal sperm function is especially important. Previous research has shown that captive cranes exhibit highly variable production and quality of semen samples produced by individual males and high levels of variation of cell concentration and motility across different species. Cyclic adenosine monophosphate (cyclic AMP) has been implicated in regulating sperm function, such as cell motility, and may affect an individual’s ability …

Surveillance Of Anaplasma Marginale In Arkansas Beef Cattle Herds, Gabriel Apple

Animal Science Undergraduate Honors Theses

Anaplasmosis is an economically devastating disease in cattle that is caused by the rickettsial pathogen Anaplasma marginale. It is estimated that this parasitic bacterium causes over $300 million in expenses for the U.S. cattle industry annually. In Arkansas, the beef cattle industry is the fifth largest agricultural commodity in the state, thus necessitating a better understanding of this disease along with its prevalence. In this study, both polymerase chain reaction (PCR) and competitive enzyme-linked immunosorbent assay (ELISA) tests were used to determine the prevalence of A. marginale infection in Arkansas beef cattle on pasture in the six commonly known geographical …

Application Of An Indirect Mila Elisa For The Detection Of Mycoplasma Bovis Antibodiesin Bovine Milk, Abd Al Bar Al Farha, Nadeeka Wawegama, Farhid Hemmatzadeh, Simon Firestone, Jamie Moffat, Gholam Ali Kojouri, Ania Ahaniazari, Reza Amanollahi, Andrew Hoare, Kiro Petrovski

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to detect Mycoplasma bovis specific antibodies using a recently reported MilA ELISA with the aim to detect M. bovis antibodies in milk. An indirect ELISA, based on a recombinant fragment of the Mycoplasma immunogenic lipase A (MilA) protein, was conducted on 291 milk samples for the detection of M. bovis antibodies. Samples were also tested with conventional Mycoplasma culture and M. bovis PCR. Samples were collected from individual cows from 2 commercial dairy herds in South Australia. Of 291 samples tested, 68 (23.4%) were detected positive for M. bovis antibodies, 150 (51.5%) were positive …

The Development Of A Fecal Glucocorticoid Metabolite Assay To Assess Stress In Captive Breeding Populations Of New England Cottontail Rabbits, Kianna Lee Taylor

Senior Honors Projects

The topic for this research project was based on the problem of the diminishing population of the wild New England Cottontail rabbits. Over the last 50 years, the population of these once very common rabbits has decreased to only inhabit New England and parts of New York (1). This is less than one-fifth of the geographic range they once inhabited (1). Roger Williams Park Zoo currently has a New England Cottontail breeding program that has experienced limited success, which may be caused by the domestic environment into which this wild species is being introduced. The central objective of this study …

Evaluation Of Real-Time Pcr, Mat, And Recombinant Lipl32-Based Elisa For The Diagnosis Of Canine Leptospirosis In A Disease-Endemic South Indian State, Kerala, Siju Joseph, Mangattumuruppel Mini, Vamshi Krishna Sriram, Ramachandran Ambily, Thazhathuveettil Aravindakshan, Sreedharannair Ajithkumar

Turkish Journal of Veterinary & Animal Sciences

Canine samples (n = 425) collected from cases of suspected leptospirosis over a period of 6 years were tested using the microscopic agglutination test (MAT), recombinant LipL32-based IgM and IgG ELISA, and real-time PCR (RT PCR). A prevalence of 69.41% was recorded by MAT. Among the different tests, IgG ELISA was observed to have maximum agreement with that of MAT (k-0.810), followed by IgM ELISA (k-0.762.). RT PCR had only slight agreement with MAT (k-0.156). However, the percentage positivity of RT PCR with samples with MAT titers of 1:100 and 1:200 were 76.47% and 85.96%, respectively, indicating the reliability of …

Epidemiological Survey And Economic Significance Of Bovine Hypodermosison The Kars Plateau In The Northeast Anatolia Region Of Turkey, Gencay Taşkin Taşçi, Bariş Sari, Ni̇lgün Parmaksizoğlu Aydin, Zati̇ Vatansever, Nesli̇han Ölmez, Ati̇la Akça, Mükremi̇n Özkan Arslan

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the prevalence and seroprevalence of hypodermosis and to calculate the economic loss associated with hide damage in the cattle on the Kars Plateau, Turkey. A total of 6563 randomly selected cattle were examined by skin palpation from 1 April to 31 May 2014. A subset of 465 serum samples was collected from October to November in 2014 and analyzed using a commercial ELISA kit. The overall prevalence of hypodermosis was 16.9% in the cattle examined by skin palpation. In respect of each of the risk factors assessed, the highest prevalence rates were …

Scenario Of Porcine Cysticercosis And Human Taeniasis In Maharashtra State, India, Vilas Vaidya, Ashish Paturkar, Ravindra Zende, Mukulesh Gatne, Dhananjay Dighe, Rupesh Waghmare, Shilpa Laxmanrao Moon, Sujata Bhave, Priyanka Jadhav, Nilam Wavhal

Turkish Journal of Veterinary & Animal Sciences

Cysticercosis/taeniasis is one of the neglected parasitic zoonoses in developing countries like India. A study was conducted for a period of 7 years from 2010 to 2017 during which 13,596 pig and 1238 human samples were screened to learn the exact scenario of cysticercosis/taeniasis in the different regions of Maharashtra State. Prevalence of cysticercosis/taeniasis in pigs and humans was recorded using various diagnostic methods such as meat inspection and serological methods involving ELISA. The overall prevalence of cysticercosis in pigs was found to be 0.88% by postmortem examination and 0.9% by PCR assay, whereas prevalence of taeniasis in humans was …

Is Tb Testing Associated With Increased Blood Interferon-Gamma Levels?, Aideen E. Kennedy, Jim O'Mahony, Noel Byrne, John Macsharry, Riona G. Sayers

Department of Biological Sciences Publications

The Republic of Ireland reports a relatively low prevalence of Johne’s disease (JD) compared to international counterparts. Postulated reasons for this include a lower average herd size and a grass-based production system. Ireland also engages in high levels of bovine tuberculosis (bTB) testing. As interferon-gamma (IFN-γ) is believed to play a key role in protecting against JD, it is our hypothesis that administration of purified protein derivative (PPD), as part of the bTB test, is associated with a systemic increase in IFN-γ production, which may potentially limit clinical progression of the disease. We studied 265 cows (202 Friesian and 63 …

Evaluation Of Diagnostic Methods For The Detection Of Pestiviruses In Clinical Samples, Peli̇n Tuncer Göktuna, Kadi̇r Yeşi̇lbağ

Turkish Journal of Veterinary & Animal Sciences

There are several commonly used diagnostic methods to detect pestiviruses for routine diagnostic purposes. In the present study, we aimed to compare virus isolation-indirect immunoperoxidase monolayer assay (VI-IIPMA), antigen capture ELISA (ACE), and RT-PCR for the detection of pestiviruses in clinical samples. Out of 246 samples tested (11 serum, 119 swab, 116 tissue), 28 samples (11.39%) were positive and 218 (88.61%) were negative using the VI-IIPMA method. Using ACE, 70 samples (28.46%) were positive and 176 (71.54%) were negative. Finally, using RT-PCR analysis, we detected 19 (7.72%) positive and 227 (92.28%) negative samples. Inconsistencies were observed among results of the …

Evidence Of Foot-And-Mouth Disease Virus Excretion In The Milk Ofapparently Healthy Vaccinated Buffaloes In Islamabad, Pakistan, Hafiziftikhar Ahmed, Umer Farooq, Aamer Bin Zahur, Khalid Naeem, Asma Latif, Hamid Irshad

Turkish Journal of Veterinary & Animal Sciences

The aim of the study was to determine the seroprevalence of foot-and-mouth disease (FMD) and isolation of the FMD virus (FMDV) in the milk of apparently healthy Asian buffaloes. A cross-sectional study was conducted in periurban dairy farms (n = 20) on ≥20 animals per farm in the suburbs of Islamabad, Pakistan. Sera samples (n = 200) were collected to monitor FMDV seroconversions. The analysis of serum samples using ELISA indicated a high seroprevalence of FMD (78%; 156/200) in Asian buffaloes. Milk samples were collected from FMDV seropositive animals only, and virus-specific signals were detected in 12.2% (19/156) of the …

Comparison Of Real-Time Pcr And Elisa-Based Methods For The Detection Of Beef And Pork In Processed Meat Products, Adam T. Perestam, Kayleigh K. Fujisaki, Omar Nava, Rosalee S. Hellberg

Food Science Faculty Articles and Research

Two commonly used methodologies for species detection within processed meat products are real-time polymerase chain reaction (PCR), a DNA-based method, and enzyme-linked immunosorbent assay (ELISA), a protein-based method. In this study, a real-time PCR assay was compared to a commercial ELISA kit based on sensitivity, specificity, agreement among duplicate samples, cost, time, and ease of use. Fifteen reference samples containing known percentages (0.1–99.9%, w/w) of pork and beef were analyzed in duplicate using both methods. Thirty commercial products, including sausages, pet treats, and canned meats, were also tested in duplicate with each method. Reference sample analysis showed real-time PCR was …

Prevalence Of Bovine Viral Diarrhea Virus In Bovine Samples From The Intermountain West Of The U.S.A. - Comparison Between Age, Sex, Breed And Diagnostic Methods, David J. Wilson, T. J. Baldwin, E. J. Kelly, A. Van Wettere, G. Hullinger, J. Bunnell

Animal, Dairy, and Veterinary Science Faculty Publications

Prevalence of Bovine viral diarrhea virus (BVDV) (“detected” test results) among all bovine samples tested at the Utah Veterinary Diagnostic Laboratory from 2008 - 2013 was calculated, and results were compared by age, sex, or breed of the cattle and BVDV diagnostic test methods. Necropsies were tested for BVDV when lesions suggestive of infection were identified. Adults, juveniles and most calves were tested by antigen (Ag) capture ELISA, while fetuses and some calves were tested by real-time reverse transcriptase PCR. Cattle originated from Utah and surrounding states. Chi-square analyses were used to test for significant differences in BVDV prevalence between …

Analysis Of Johne's Disease Elisa Status And Associated Performance Parameters In Irish Dairy Cows, A. E. Kennedy, N. Byrne, A. B. Garcia, Jim O'Mahony, R. G. Sayers

Department of Biological Sciences Publications

Background

Infection with Mycobacterium avium subspecies paratuberculosis (MAP) has been associated with reductions in milk production in dairy cows and sub optimal fertility. The aim of this study was to highlight the production losses associated with testing MAP ELISA positive in Irish dairy cows. Secondary objectives included investigation of risk factors associated with testing MAP ELISA positive. A survey of management practices on study farms was also conducted, with examination of associations between management practices and herd MAP status.

Blood samples were collected from 4188 breeding animals on 22 farms. Samples were ELISA tested using the ID Screen Paratuberculosis Indirect …

Analysis Of Johne’S Disease Elisa Status And Associated Performance Parameters In Irish Dairy Cows, A. E. Kennedy, N. Byrne, A. B. Garcia, Jim O'Mahony, R. G. Sayers

Department of Biological Sciences Publications

Infection with Mycobacterium avium subspecies paratuberculosis (MAP) has been associated with reductions in milk production in dairy cows and sub optimal fertility. The aim of this study was to highlight the production losses associated with testing MAP ELISA positive in Irish dairy cows. Secondary objectives included investigation of risk factors associated with testing MAP ELISA positive. A survey of management practices on study farms was also conducted, with examination of associations between management practices and herd MAP status.

Blood samples were collected from 4188 breeding animals on 22 farms. Samples were ELISA tested using the ID Screen Paratuberculosis Indirect Screening …

Comparative Study Between Formalin-Killed Vaccine And Developed Gammairradiation Vaccine Against Mannheimia Haemolytica In Rabbits, Sahar Ahmed, Basem Ahmed, Ghada Mahmoud, Waleed Nemr, Emam Abdel Rahim

Turkish Journal of Veterinary & Animal Sciences

Mannheimia haemolytica is responsible for considerable economic losses to sheep, goats, and cattle and other livestock industries in Egypt. This study aimed to evaluate the effectiveness of a newly developed gamma irradiation vaccine against Mannheimia haemolytica in comparison to a formalin-killed vaccine. Three groups of rabbits were used in this study. Group 1 animals were inoculated with 4 × 109 bacterial cells per dose of the formalin-killed vaccine. Group 2 was inoculated with 2 × 109 bacterial cells per dose of gamma-irradiated vaccine. Group 3 (control group) was injected with 2 mL of sterile PBS. The vaccines were injected subcutaneously …

Serological Survey For Antibodies Against Mycobacterium Avium Complexin Hunting Dogs In East-Central Portugal, Ana Claudia Coelho, Manuela Matos, Luis Figueira, Maria De Lurdes Pinto, Juan Garcia Diez, Ana Catarina Matos

Turkish Journal of Veterinary & Animal Sciences

The Mycobacterium avium complex (MAC) causes infections and diseases in humans, birds, livestock, and wildlife, among others. In dogs, MAC infection is not frequent; however, due to its potential zoonotic characteristics, mainly in immunocompromised patients, the study of MAC epidemiology is necessary. The aim of this work was to perform a study to detect the presence of antibodies against MAC in serum samples of 53 hunting dogs in the Idanha-a-Nova municipality of East-Central Portugal. A total of 23 dogs were seropositive by commercial ELISA test (43.4%, 95% CI: 30.1%-56.7%). Factors such as age, breed, and sex were not significant. The …

Early Pregnancy Diagnosis Using A Commercial Elisa Test Based On Pregnancy-Associated Glycoproteins In Holstein-Friesian Heifers And Lactating Cows, Mehmet Sali̇h Kaya, Mehmet Köse, Faruk Bozkaya, Hasan Mutlu, Eyyüp Hakan Uçar, Mehmet Osman Atli

Turkish Journal of Veterinary & Animal Sciences

In this study, we aimed to investigate the efficacy of a commercial ELISA test kit for detecting pregnancy-associated glycoproteins (PAGs) in peripheral blood for early pregnancy diagnosis and compare plasma PAG levels during early pregnancy in both Holstein-Friesian heifers and lactating cows. A total of 231 plasma samples were collected from heifers and lactating cows on days 25, 28, and 32 after insemination. Pregnancies were confirmed 30 days after the collection of plasma samples. Plasma PAG levels were measured using a commercial ELISA test kit for diagnosing bovine pregnancy. The effects of examination date and animal status (heifers vs. lactating …

Thermal Inactivation Of Shiga Toxin-Producing Escherichia Coli In Foods, Malcond David Valladares

Doctoral Dissertations

Emerging non-O157 Shiga toxin-producing Escherichia coli (STEC) were recently added to the zero tolerance policy by the USDA-FSIS. Therefore, the precise characterization of their thermal inactivation kinetics in different foods and the effect of stress on thermal inactivation are needed. This research aimed at determining the heat inactivation kinetics of non-O157 and O157 STECs in buffer and model food matrices and the effects of DnaK levels on thermal resistance after acid and heat-shock. Thermal inactivation was carried out in either in 2-ml glass vials or nylon vacuum-sealed bags for buffer and food (spinach, ground-beef, turkey deli-meat, pasta) samples, respectively. Vials …

Enzyme-Linked Immunosorbent Assay For The Detection Of Antibodies Against The Extracellular Domain Of The Oxaa Membrane Protein Of Mycoplasma Suis, Lijun Jia, Mingming Liu, Shoufa Zhang, Lei Zhang, Huanping Guo, Jixu Li

Turkish Journal of Veterinary & Animal Sciences

Epidemics of swine infected with Mycoplasma suis, a hemotrophic pathogen, cause substantial economic losses to the swine industry worldwide. The complementary DNA sequences encoding two predicted extracellular domains of the OxaA protein of M. Suis were obtained using polymerase chain reaction and standard molecular cloning methods. The OxaA domains were expressed separately in Escherichia coli as glutathione-S-transferase (GST) fusion proteins, designated as GST-A and GST-B. The antigenicity of the GST-A and -B recombinant proteins was confirmed by western blotting. The GST-A and -B proteins were approximately 33.9 kDa and 34.6 kDa in size, respectively. An enzyme-linked immunosorbent assay (ELISA) was …

Modulation Of Vaccine-Induced Responses By Anthelmintic Treatment In Ponies, Emily Rubinson

Theses and Dissertations--Medical Sciences

Vaccines and anthelmintics induce an inflammatory response in equids. Since they are commonly given concurrently, it is practical to study any interaction between them. This study evaluated whether IVM and PYR would modulate the acute phase inflammatory response, the systemic gene expression of pro-inflammatory cytokines, and vaccine-specific titers induced by WNV, EHV, and KLH vaccines. Naturally-infected, yearling ponies were sorted by gender, then fecal epgs. They were randomly assigned to three treatment groups: IVM, PYR, and control. All ponies received vaccinations intramuscularly on days 0 and 29. Whole blood, serum, and plasma samples were collected 1, 3, and 14 days …

Co-Infection Of Water Buffaloes In Punjab, Pakistan, With Neospora Caninum And Brucella Abortus, Amar Nasir, Muhammad Ashraf, Abdul Shakoor, Muhammad Adil, Tariq Abbas, Muhammad Kashif, Muhammad Younus Rana, Michael Philipp Reichel

Turkish Journal of Veterinary & Animal Sciences

Neosporosis and brucellosis are 2 well-known diseases causing bovine abortion. This study determined co-infection of Neospora caninum and Brucella abortus in buffaloes for the first time in Pakistan. A monoclonal antibody based competitive ELISA was used to test buffalo sera (n = 312) for N. caninum specific antibodies, while rose bengal plate test assessed B. abortus antibodies. N. caninum and B. abortus prevalence was 42.8% (±6.7, 95% C.I.) and 12.2% (±3.6, 95% C.I.), respectively. Buffaloes >3 years old exhibited higher prevalence than younger ones. Co-infection was particularly significant as 13.2% of Neospora infected buffaloes were also Brucella infected with an …

Comparative Diagnostic Methods For Canine Ehrlichiosis, Chetan Parmar, Riddhi Pednekar, Anant Jayraw, Mukulesh Gatne

Turkish Journal of Veterinary & Animal Sciences

The efficacy of blood and buffy coat smear examination, commercially available dot-enzyme-linked immunosorbent assay (ELISA) kit, and nested polymerase chain reaction (PCR) was evaluated in the diagnosis of canine ehrlichiosis in 40 dogs exhibiting symptoms suggestive of the disease. The intracytoplasmic rickettsial organisms were seen in the smears of only 1 dog (2.5%) with an extremely low level of parasitemia. Nested PCR with 16S rRNA revealed characteristic 378 bp bands indicating positive reaction in 8 (20.0%) dogs and sera samples of 21 (52.5%) dogs revealed anti-Ehrlichia antibodies. The dog that revealed organisms in the blood also showed positive results with …

Scrapie Cases In The Northern Cyprus, Aydin Gürel, Ahmet Gülçubuk, Nuri̇ Turan, Christopher Richard Helps, Hüseyi̇n Yilmaz

Turkish Journal of Veterinary & Animal Sciences

Scrapie is a fatal neurodegenerative disease affecting small ruminants that causes economic losses in many countries. It is categorized together with other fatal neurodegenerative diseases as a transmissible spongiform encephalopathy. The aim of this study was to investigate clinical cases and the cause of death in a flock of sheep from Northern Cyprus (Turkish Republic of Northern Cyprus) suspected of having scrapie. Brains from 4 sheep showing clinical signs of classical scrapie, out of a flock of 200 animals, were analyzed by histopathological examination and infective prion enzyme-linked immunosorbent assay (ELISA). The 4 affected sheep were 2-3 years of age …

Immunokinetics Of Theileria Equi Specific Antibodies: A Comparison In Serial And Single Dilution Elisa Antibody End Titres, Yogesh Kumar, Dharam Vir Malhotra, Anil Kumar Nichani, Ashok Kumar, Shruti Dhar, Sanjay Kumar

Turkish Journal of Veterinary & Animal Sciences

Theileria equi, a haemoprotozoan of equids, is responsible for endemic infection/latency in tropical/subtropical parts of the world. The present study investigated the immunokinetics of T. equi infection in experimental animals and the diagnostic applicability of single dilution ELISA vis-à-vis conventional serial dilution ELISA. Sequential serum samples were collected up to 90 days postinfection (PI) from 4 donkeys experimentally infected with T. equi. Immunokinetics of T. equi antibodies end titre were determined by serial and single dilution ELISA. A significant rise in initial ELISA antibody end titre (1:800 in serial dilution ELISA and from 1:600 to 1:650 in single dilution ELISA) …

Evaluation Of Canine Anti-Leishmania Igg Subclasses And Their Relation With Skin Signs In Naturally Infected Dogs In The Northwest Of Iran, Ali Shabestari Asl, Majid Sharifi, Mehdi Mohebali, Zabihollah Zarei, Zohreh Babaloo, Behnaz Akhoundi

Turkish Journal of Veterinary & Animal Sciences

Canine leishmaniasis (CanL) is a severe, intracellular parasitic zoonosis and is an endemic complaint in Iran. Dogs are considered the reservoir host for Leishmania infantum in the Old World. The aims of this study were to evaluate the relation between anti-Leishmania antibody titers (IgG) and skin symptoms of CanL in naturally affected dogs and to assess the correlation of IgG_1 and IgG_2 subclasses of titers with these symptoms. Anti-Leishmania antibody titers were evaluated in 100 dogs' blood samples with the direct agglutination test (DAT) and enzyme-linked immunosorbent assay (ELISA) methods. Based on DAT results, 45 samples were positive. Twenty-five of …

Toxinotyping Of Clostridium Perfringens Isolates By Elisa And Pcr From Lambs Suspected Of Enterotoxemia, Hasan Hüseyi̇n Hadi̇mli̇, Osman Ergani̇ş, Zafer Sayin, Zeki̇ Aras

Turkish Journal of Veterinary & Animal Sciences

Clostridium perfringens is a gram-positive, anaerobic bacterium that causes a wide range of diseases in humans and animals. The purposes of this study were to determine, using the enzyme-linked immunosorbent assay (ELISA), the clostridial toxins in intestinal samples of lambs with suspected enterotoxemia; to conduct molecular typing of C. perfringens isolates; and to investigate the presence of the genes of 4 major toxins (\alpha, \beta, \epsilon, and \iota) in the isolates by polymerase chain reaction (PCR). According to the ELISA results, clostridial toxins were determined in 32 intestinal samples. Of the samples, 13 (40.62%) were C. perfringens type A, 1 …

Aflatoxin M1 Levels In Retail Yoghurt And Ayran In Erzurum In Turkey, Meryem Aydemi̇r Atasever, Mustafa Atasever, Korhan Özturan

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to determine the presence and levels of aflatoxin M1 (AFM1) in yoghurt and ayran consumed in the province of Erzurum, Turkey. For this purpose, a total of 80 yoghurt samples and 80 ayran samples were randomly obtained from markets. The samples' AFM1 content and concentrations were examined by competitive ELISA method. AFM1 at detectable level (5 ng/kg) was found in 87.5% of the yoghurt samples and in 90.0% of the ayran samples. AFM1 levels in 16 (20%) yoghurt and in 11 (13.6%) ayran samples exceeded the maximum tolerable limit of the Turkish Food Codex. The …

Aflatoxin M1 Contamination In Cow’S Milk In Kayseri (Central Turkey), Hi̇kmet Mavuş Buldu, A. Nedret Koç, Güven Uraz

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to determine the levels of aflatoxin M1 (AFM1) in the milk of cows in the villages of Kayseri province, Turkey. Ninety milk samples were investigated for AFM1 contamination using the enzyme linked immunoassay (ELISA). The mean value of AFM1 levels was 59.9 ng/L, 95% confidence interval was 54.4-65.5 ng/L, and standard deviation was 26.6 ng/L. For 63 samples (70%) the values were significantly above the limit permitted by European Union criteria (EU) and the Turkish Food Codex (TFC) (P < 0.05). However, the differences in AFM1 levels between villages were not significant (P > 0.05). In conclusion, since the level of AFM1 in the milk of cows was higher …

Molecular And Serological Evidence For Anaplasma Infection In Yaks, Miling Ma, Zhijie Liu, Guiquan Guan, Youquan Li, Jianxun Luo, Hong Yin

Turkish Journal of Veterinary & Animal Sciences

Bovine anaplasmosis caused by Anaplasma marginale is considered to be an important disease in China; large-scale investigations have not been carried out since the 1990s, however, and whether or not yaks can be infected by A. marginale has never been investigated. In this study, 2 yaks were successfully infected by the inoculation of blood infected with A. marginale under experimental conditions. Furthermore, an investigation of 482 field serum samples from yaks using an enzyme-linked immunosorbent assay (ELISA) with the recombinant major surface protein 5 (MSP5) showed that 84.4% of the results were positive. In consideration of previously determined ecological and …