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Phorbol Ester Activation Of An Nhe-Like Electroneutral Na+ /H+ Antiporter In Isolated E-Cells Of Lobster (Homarus Americanus) Hepatopancreas, Jeff Duerr, Gregory A. Ahearn
Phorbol Ester Activation Of An Nhe-Like Electroneutral Na+ /H+ Antiporter In Isolated E-Cells Of Lobster (Homarus Americanus) Hepatopancreas, Jeff Duerr, Gregory A. Ahearn
Faculty Publications - Department of Biological & Molecular Science
The basolateral membrane of Atlantic lobster (Homarus americanus) epithelium possesses an electroneutral Na+ /H+ antiporter that functionally resembles members of the vertebrate NHE family. Regulatory mechanisms of this antiporter in purified hepatopancreatic E-cell suspensions, produced with a centrifugal elutriation technique, were investigated. Suspensions routinely consisted of greater than 95% E-cells displaying greater than 90% viability. Intracellular pH (pHi) was monitored by loading cells with the fluorescent dye 2´,7´-bis(carboxyethyl)-5,6- carboxyfluorescein (BCECF), and placing suspensions in a spectrofluorometer. Recovery from induced acid-loading was mediated by a Na+ -dependent, dimethylamiloride-sensitive proton efflux. Antiport activation was a sigmoidal function of pHi at values below …