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Biointeraction Analysis By High-Performance Affinity Chromatography: Kinetic Studies Of Immobilized Antibodies, Mary Anne Nelson, Annette C. Moser, David S. Hage 2010 University of Nebraska - Lincoln

Biointeraction Analysis By High-Performance Affinity Chromatography: Kinetic Studies Of Immobilized Antibodies, Mary Anne Nelson, Annette C. Moser, David S. Hage

David Hage Publications

A system based on high-performance affinity chromatography was developed for characterizing the binding, elution and regeneration kinetics of immobilized antibodies and immunoaffinity supports. This information was provided by using a combination of frontal analysis, split-peak analysis and peak decay analysis to determine the rate constants for antibody–antigen interactions under typical sample application and elution conditions. This technique was tested using immunoaffinity supports that contained monoclonal antibodies for 2,4-dichlorophenoxyacetic acid (2,4-D). Association equilibrium constants measured by frontal analysis for 2,4-D and related compounds with the immobilized antibodies were 1.7–12 × 106 M−1 at pH ...


Climate And More Sustainable Cities: Climate Information For Improved Planning And Management Of Cities (Producers/Capabilities Perspective), C.S.B. Grimmond, Matthias Roth, Tim R. Oke, Y. C. Au, M. Best, R. Betts, Gregory R. Carmichael, Helen Cleugh, Walter F. Dabberdt, Rohinton Emmanuel, E. Freitas, K. Fortuniak, Steven Hanna, Petra M. Klein, Laurence S. Kalkstein, C. H. Liu, Alex Nickson, David Pearlmutter, David J. Sailor, James Voogt 2010 King's College London

Climate And More Sustainable Cities: Climate Information For Improved Planning And Management Of Cities (Producers/Capabilities Perspective), C.S.B. Grimmond, Matthias Roth, Tim R. Oke, Y. C. Au, M. Best, R. Betts, Gregory R. Carmichael, Helen Cleugh, Walter F. Dabberdt, Rohinton Emmanuel, E. Freitas, K. Fortuniak, Steven Hanna, Petra M. Klein, Laurence S. Kalkstein, C. H. Liu, Alex Nickson, David Pearlmutter, David J. Sailor, James Voogt

Mechanical and Materials Engineering Faculty Publications and Presentations

In the last two decades substantial advances have been made in the understanding of the scientific basis of urban climates. These are reviewed here with attention to sustainability of cities, applications that use climate information, and scientific understanding in relation to measurements and modelling. Consideration is given from street (micro) scale to neighbourhood (local) to city and region (meso) scale. Those areas where improvements are needed in the next decade to ensure more sustainable cities are identified. High-priority recommendations are made in the following six strategic areas: observations, data, understanding, modelling, tools and education. These include the need for more ...


Gis Data: Fairfax County And The City Of Alexandria, Virginia Shoreline Inventory Report, Marcia Berman, Harry Berquist, Sharon Killeen, Carl Hershner, Karinna Nunez, Karen Reay, Tamia Rudnicky 2010 Virginia Institute of Marine Science

Gis Data: Fairfax County And The City Of Alexandria, Virginia Shoreline Inventory Report, Marcia Berman, Harry Berquist, Sharon Killeen, Carl Hershner, Karinna Nunez, Karen Reay, Tamia Rudnicky

Data

The data inventory developed for the Shoreline Inventory is based on a three‑tiered shoreline assessment approach. In most cases this assessment characterizes conditions that can be observed from a small boat navigating along the shoreline. The three tiered shoreline assessment approach divides the shorezone into three regions: 1) the immediate riparian zone, evaluated for land use; 2) the bank, evaluated for height, stability, cover and natural protection; and 3) the shoreline, describing the presence of shoreline structures for shore protection and recreational purposes. Hand-held GPS units are used to log features observed in the field.

Three GIS coverages are ...


Evolving Models: A Density-Based Approach To Modeling Sexual Dimorphism And Adaptive Speciation, Audrey Smith 2010 Utah State University

Evolving Models: A Density-Based Approach To Modeling Sexual Dimorphism And Adaptive Speciation, Audrey Smith

All Graduate Plan B and other Reports

In this paper, we begin by extending existing deterministic and individual-based ecological models for sexual dimorphism and adaptive speciation into density-based mathematical models describing the density or number of individuals with various trait values, or phenotypes. These density-based models describe the dynamics of a population of males and females using both clonal and sexual reproduction. Each generation, the populations are subject to mating, mutation, and ecological dynamics including infraspecific competition and carrying capacity of the environment. By avoiding individual-based models, we are able to avoid simulations and instead achieve repeatable results.

Implementing these models numerically, we are able to show ...


Numerical Solution Of The Five-Moment Ideal Two-Fluid Equations In One Dimension, Marcus Scott 2010 Utah State University

Numerical Solution Of The Five-Moment Ideal Two-Fluid Equations In One Dimension, Marcus Scott

All Graduate Plan B and other Reports

Plasmas are frequently treated as a single conducting fluid and modeled using the equations of magnetohydrodynamics. However, this regime works better for low-frequency plasmas. High-frequency plasmas may be modeled using the principles of kinetic theory. For plasmas with frequencies between these two extremes, a two-fluid approach can yield better results. In 2006, Ammar Hakim mathematically modeled a plasma with a set of equations called the five-moment ideal two-fluid equations. An attempt is made reproduce those results. A derivation of this set of equations by taking moments of the Boltzmann equation is presented. Electric and magnetic fields contribute to the source ...


Assessing North American Influenza Dynamics With Hierarchical Spatio-Temporal Models, Jessica Anderson 2010 Utah State University

Assessing North American Influenza Dynamics With Hierarchical Spatio-Temporal Models, Jessica Anderson

All Graduate Plan B and other Reports

We present a general statistical modeling framework to characterize continental-level influenza dynamics in the United States for the purposes of examining state-level epidemiological sources and sinks. The methods we describe depend directly on state-level influenza data that are updated weekly and available on the internet. Advances in search engine query analysis have provided powerful new tools for collecting epidemiological data and, when used in conjunction with sophisticated statistical models, allow for the identification and quantification of the flow of influenza across the continental United States. Our proposed methods, when conditioned on this comprehensive search query product, can provide unprecedented scientific ...


Assessing The Precision And Accuracy In A Small Sample Of Actical Devices, Peter Sherick 2010 Utah State University

Assessing The Precision And Accuracy In A Small Sample Of Actical Devices, Peter Sherick

All Graduate Plan B and other Reports

Actigraphy is an increasingly popular approach in medicine to assess patient activity levels in a variety of scenarios. The devices are essentially accelerometers encased in a write-watch type assembly. This project sought to determine the device precision and accuracy for the Actical model. In a sample of four Acticals, it was found that intra-device variability was minimal. However, one device was found to be statistically biased in comparison to the other three. This bias could have adverse effects on aggregated or magnitude dependent data analysis. Also, inter-device comparisons may be problematic.


A Comparison Of Prediction Methods Of Functional Autoregressive Time Series, Devin Didericksen 2010 Utah State University

A Comparison Of Prediction Methods Of Functional Autoregressive Time Series, Devin Didericksen

All Graduate Plan B and other Reports

Functional data analysis (FDA) is a relatively new branch of statistics that has seen a lot of expansion recently. With the advent of computer processing power and more efficient software packages we have entered the beginning stages of applying FDA methodology and techniques to data. Part of this undertaking should include an empirical assessment of the effectiveness of some of the tools of FDA, which are sound on theoretical grounds. In a small way, this project helps advance this objective.

This work begins by introducing FDA, scalar prediction techniques, and the functional autoregressive model of order one - FAR(1). Two ...


Nanoporous High Surface Area Silicas With Chelating Groups For Heavy Metal Ion Adsorption From Aqueous Solution, Kothalawalage Nuwan Kothalawala 2010 Eastern Illinois University

Nanoporous High Surface Area Silicas With Chelating Groups For Heavy Metal Ion Adsorption From Aqueous Solution, Kothalawalage Nuwan Kothalawala

Masters Theses

No abstract provided.


Ditopic Dithiophosphonates And Their Related Rotaxanes, Robert Ray Baum Jr. 2010 Eastern Illinois University

Ditopic Dithiophosphonates And Their Related Rotaxanes, Robert Ray Baum Jr.

Masters Theses

No abstract provided.


Reproductive Biology Of Male Brown Treesnakes (Boiga Irregularis) On Guam, Tom Mathies, John A. Cruz, Valentine A. Lance, Julie A. Savidge 2010 USDA-APHIS-Wildlife Services

Reproductive Biology Of Male Brown Treesnakes (Boiga Irregularis) On Guam, Tom Mathies, John A. Cruz, Valentine A. Lance, Julie A. Savidge

USDA National Wildlife Research Center - Staff Publications

Reproductive biology of males in the Guam population of the Brown Treesnake, Boiga irregularis, was investigated through monthly examinations of the urogenital system organs and plasma testosterone levels. All males examined during the 12 consecutive months of the study were spermatogenic and had sperm in the ductus epididymis and ductus deferens. No evidence of testicular recrudescence or regression was observed. Testis mass did not vary among months. Epithelial height of the kidney sexual segment was the only feature examined that varied significantly among months, with lowest heights observed in May through July. Despite this variation, the sexual segment in all ...


Contraceptive Efficacy Of A Novel Intrauterine Device (Iud) In White-Tailed Deer, Karl D. Malcolm, Timothy R. Van Deelen, David Drake, Darrel J. Kesler, Kurt C. VerCauteren 2010 University of Wisconsin, Madison, Department of Forest and Wildlife Ecology

Contraceptive Efficacy Of A Novel Intrauterine Device (Iud) In White-Tailed Deer, Karl D. Malcolm, Timothy R. Van Deelen, David Drake, Darrel J. Kesler, Kurt C. Vercauteren

USDA National Wildlife Research Center - Staff Publications

Overabundant white-tailed deer (Odocoileus virginianus) pose risks to property, health, and safety of human beings. Public concerns about lethal management can impair efforts to address these issues, particularly in urban settings. Several techniques developed for reducing reproductive output of deer have limited utility because they require repeated dosing to achieve permanent effect and face uncertain regulatory approval for use beyond experimentation. From 10 August 2006 through 30 December 2007, we evaluated the contraceptive efficacy of copper-containing intrauterine devices (IUDs) implanted trans-cervically in white-tailed deer at the E.S. George Reserve in Pinckney, Michigan. Intrauterine devices were implanted before (n = 9 ...


Antibodies To Influenza And West Nile Viruses In Horses In Mexico, M. A. Loroño-Pino, J. A. Farfan-Ale, J. E. Garcia-Rejon, M. Lin, E. Rosado-Paredes, F. I. Puerto, A. Bates, J. J. Root, A. B. Franklin, H. J. Sullivan, B. J. Blitvich 2010 Laboratorio de Arbovirologia, Centro de Investigaciones Regionales ‘Dr Hideyo Noguchi’, Universidad Autónoma de Yucatán, Mérida, Yucatán, CP 97000, Mexico

Antibodies To Influenza And West Nile Viruses In Horses In Mexico, M. A. Loroño-Pino, J. A. Farfan-Ale, J. E. Garcia-Rejon, M. Lin, E. Rosado-Paredes, F. I. Puerto, A. Bates, J. J. Root, A. B. Franklin, H. J. Sullivan, B. J. Blitvich

USDA National Wildlife Research Center - Staff Publications

INFLUENZA A virus (IAV) (family Orthomyxoviridae) is a highly infectious respiratory pathogen of birds and mammals, including human beings and horses (Palese and Shaw 2007). The virus is classified into different subtypes based on the antigenic properties of the haemagglutinin (HA) and neuraminidase (NA) proteins. Sixteen HA subtypes (H1 to H16) and nine NA subtypes (N1 to N9) have been identified (Fouchier and others 2005). Two subtypes, H3N8 and H7N7, have been isolated from horses. The H7N7 subtype was first isolated from a horse in Czechoslovakia in 1956 (Prague/56) (Sovinova and others 1958), and the H3N8 subtype was first ...


Evaluation Of Feral Swine- Specific Feeder Systems, David B. Long, Tyler A. Campbell, Giovanna Massei 2010 United States Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services, National Wildlife Research Center, Florida Field Station

Evaluation Of Feral Swine- Specific Feeder Systems, David B. Long, Tyler A. Campbell, Giovanna Massei

USDA National Wildlife Research Center - Staff Publications

Feral swine (Sus scrofa) have been introduced across many portions of the globe, including rangeland ecosystems of the United States. Feral swine populations are expanding because of their adaptability, high reproductive potential, and because they are (accidentally and intentionally) released by humans. Today, feral swine are the most abundant exotic ungulate in the United States.

Rangeland ecosystems are impacted by feral swine primarily through soil disturbance caused by rooting activities. Within these systems, natural disturbances (e.g., burrowing, grazing by native animals, and periodic fire) generally increase or maintain species diversity. However, rooting by feral swine often occurs at intensities ...


Acetaminophen As An Oral Toxicant For Nile Monitor Lizards (Varanus Niloticus) And Burmese Pythons (Python Molurus Bivittatus), Richard E. Mauldin, Peter J. Savarie 2010 USDA-APHIS-Wildlife Services

Acetaminophen As An Oral Toxicant For Nile Monitor Lizards (Varanus Niloticus) And Burmese Pythons (Python Molurus Bivittatus), Richard E. Mauldin, Peter J. Savarie

USDA National Wildlife Research Center - Staff Publications

Context. Invasive species are a growing global problem. Biological invasions can result in numerous harmful impacts on local ecologies, and non-native herpetofauna are frequently ignored. Nile monitor lizards (Varanus niloticus) and Burmese pythons (Python molurus bivittatus, recently reassessed as Python bivittatus bivittatus), have become established in southern Florida. Both are large, semi-aquatic predators that pose serious threats to a variety of threatened and endangered species, as well as to the unique ecology of the area.

Aims. Acetaminophen (CAS#103-90-2), a lethal oral toxicant for the invasive brown treesnake (Boiga irregularis) on Guam, was investigated as a possible toxicant in juvenile ...


Seeking A Second Opinion: Uncertainty In Disease Ecology, Brett T. McClintock, James D. Nichols, Larissa L. Bailey, Darryl I. MacKenzie, William. L. Kendall, Alan B. Franklin 2010 USGS Patuxent Wildlife Research Center

Seeking A Second Opinion: Uncertainty In Disease Ecology, Brett T. Mcclintock, James D. Nichols, Larissa L. Bailey, Darryl I. Mackenzie, William. L. Kendall, Alan B. Franklin

USDA National Wildlife Research Center - Staff Publications

Analytical methods accounting for imperfect detection are often used to facilitate reliable inference in population and community ecology. We contend that similar approaches are needed in disease ecology because these complicated systems are inherently difficult to observe without error. For example, wildlife disease studies often designate individuals, populations, or spatial units to states (e.g., susceptible, infected, post-infected), but the uncertainty associated with these state assignments remains largely ignored or unaccounted for. We demonstrate how recent developments incorporating observation error through repeated sampling extend quite naturally to hierarchical spatial models of disease effects, prevalence, and dynamics in natural systems. A ...


A Silent Enzootic Of An Orthopoxvirus In Ghana, West Africa: Evidence For Multi-Species Involvement In The Absence Of Widespread Human Disease, Mary G. Reynolds, Darin S. Carroll, Victoria A. Olson, Christine Hughes, Jack Galley, Anna Likos, Joel M. Montgomery, Richard Suu-Ire, Mubarak O. Kwasi, J. Jeffrey Root, Zach Braden, Jason Abel, Cody Clemmons, Russell Regnery, Kevin Karem, Inger K. Damon 2010 USDA/APHIS/WS National Wildlife Research Center

A Silent Enzootic Of An Orthopoxvirus In Ghana, West Africa: Evidence For Multi-Species Involvement In The Absence Of Widespread Human Disease, Mary G. Reynolds, Darin S. Carroll, Victoria A. Olson, Christine Hughes, Jack Galley, Anna Likos, Joel M. Montgomery, Richard Suu-Ire, Mubarak O. Kwasi, J. Jeffrey Root, Zach Braden, Jason Abel, Cody Clemmons, Russell Regnery, Kevin Karem, Inger K. Damon

USDA National Wildlife Research Center - Staff Publications

Human monkeypox has never been reported in Ghana, but rodents captured in forested areas of southern Ghana were the source of the monkeypox virus introduced into the United States in 2003. Subsequent to the outbreak in the United States, 204 animals were collected from two commercial trapping sites in Ghana. Animal tissues were examined for the presence of orthopoxvirus (OPXV) DNA using a real-time polymerase chain reaction, and sera were assayed for antibodies against OPXV. Animals from five genera (Cricetomys , Graphiurus , Funiscirus, and Heliosciurus ) had antibodies against OPXV, and three genera (Cricetomys , Graphiurus , and Xerus) had evidence of OPXV DNA ...


Test Of Localized Management For Reducing Deer Browsing In Forest Regeneration Areas, Brad F. Miller, Tyler A. Campbell, Ben Laseter, W. Mark Ford, Karl Miller 2010 Arkansas Game and Fish Commission

Test Of Localized Management For Reducing Deer Browsing In Forest Regeneration Areas, Brad F. Miller, Tyler A. Campbell, Ben Laseter, W. Mark Ford, Karl Miller

USDA National Wildlife Research Center - Staff Publications

White-tailed deer (Odocoileus virginianus) browsing in forest regeneration sites can affect current and future stand structure and species composition. Removal of deer social units (localized management) has been proposed as a strategy to alleviate deer overbrowsing in forest systems. We conducted an experimental localized removal in a high-density deer population in the central Appalachians of West Virginia, USA, during winter 2002. We removed 51 deer within a 1.1-km2 area that encompassed 2 forest regeneration sites (14 ha). During the summer following removal, we detected decreases in distance from the removal area in 8 of 30 (26.7%) adult ...


Fine-Scale Genetic And Social Structuring In A Central Appalachian White-Tailed Deer Herd, Brad F. Miller, Randy W. DeYoung, Tyler A. Campbell, Benjamin R. Laseter, W. Mark Ford, Karl V. Miller 2010 University of Georgia, Athens

Fine-Scale Genetic And Social Structuring In A Central Appalachian White-Tailed Deer Herd, Brad F. Miller, Randy W. Deyoung, Tyler A. Campbell, Benjamin R. Laseter, W. Mark Ford, Karl V. Miller

USDA National Wildlife Research Center - Staff Publications

Spatial genetic structure in white-tailed deer (Odocoileus virginianus) has been examined at regional scales, but genetic markers with the resolution to detect fine-scale patterns have appeared only recently. We used a panel of microsatellite DNA markers, radiotelemetry data, and visual observations of marked deer to study fine-scale social and genetic structure in a high-density population of white-tailed deer (12–20 deer/km2). We collected genetic data on 229 adult females, 102 of which were assigned to 28 social groups. Our results were consistent with the conceptual model of white-tailed deer social structure, where philopatric females form social groups composed ...


The Versatility Of Graded Acoustic Measures In Classification Of Predation Threats By The Tufted Titmouse Baeolophus Bicolor: Exploring A Mixed Framework For Threat Communication, Kathryn E. Sieving, Stacia A. Hetrick, Michael L. Avery 2010 Department of Wildlife Ecology and Conservation, University of Florida

The Versatility Of Graded Acoustic Measures In Classification Of Predation Threats By The Tufted Titmouse Baeolophus Bicolor: Exploring A Mixed Framework For Threat Communication, Kathryn E. Sieving, Stacia A. Hetrick, Michael L. Avery

USDA National Wildlife Research Center - Staff Publications

Many mammal and bird species respond to predator encounters with alarm vocalizations that generate risk-appropriate responses in listeners. Two conceptual frameworks are typically applied to the information encoded in alarm calls and to associated anti-predator behaviors. ‘Functionally referential’ alarm systems encode nominal classes or categories of risk in distinct call types that refer to distinct predation-risk situations. ‘Risk-based’ alarms encode graded or ranked threat-levels by varying the production patterns of the same call types as the urgency of predation threat changes. Recent work suggests that viewing alarm-response interactions as either referential or risk-based may oversimplify how animals use information in ...


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