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Utilizing Protein-Lean Coproducts From Corn Containing Recombinant Pharmaceutical Proteins For Ethanol Production, Ilankovan Paraman, Lorena Beatriz Moeller, M. Paul Scott, Kan Wang, Charles E. Glatz, Lawrence A. Johnson 2010 Iowa State University

Utilizing Protein-Lean Coproducts From Corn Containing Recombinant Pharmaceutical Proteins For Ethanol Production, Ilankovan Paraman, Lorena Beatriz Moeller, M. Paul Scott, Kan Wang, Charles E. Glatz, Lawrence A. Johnson

Chemical and Biological Engineering Publications

Protein-lean fractions of corn (maize) containing recombinant (r) pharmaceutical proteins were evaluated as a potential feedstock to produce fuel ethanol. The levels of residual r-proteins in the coproduct, distillers dry grains with solubles (DDGS), were determined. Transgenic corn lines containing recombinant green fluorescence protein (r-GFP) and a recombinant subunit vaccine of Escherichia coli enterotoxin (r-LTB), primarily expressed in endosperm, and another two corn lines containing recombinant human collagen (r-CIα1) andr-GFP, primarily expressed in germ, were used as model systems. The kernels were either ground and used for fermentation or dry fractionated to recover germ-rich ...


Impervious Surfaces Mapping Using High Resolution Satellite Imagery, Tahmina Shirmeen 2010 University of Mississippi

Impervious Surfaces Mapping Using High Resolution Satellite Imagery, Tahmina Shirmeen

Electronic Theses and Dissertations

In recent years, impervious surfaces have emerged not only as an indicator of the degree of urbanization, but also as an indicator of environmental quality. As impervious surface area increases, storm water runoff increases in velocity, quantity, temperature and pollution load. Any of these attributes can contribute to the degradation of natural hydrology and water quality. Various image processing techniques have been used to identify the impervious surfaces, however, most of the existing impervious surface mapping tools used moderate resolution imagery. In this project, the potential of standard image processing techniques to generate impervious surface data for change detection analysis ...


Holocene Landscape Response To Seasonality Of Storms In The Mojave Desert, David M. Miller, Kevin M. Schmidt, Shannon A. Mahan, John P. McGeehin, Lewis A. Owen, John A. Barron, Frank Lehmkuhl, Rene Lohrer 2010 U.S. Geological Survey, 345 Middlefield Road, MS-973, Menlo Park, CA 94025, USA

Holocene Landscape Response To Seasonality Of Storms In The Mojave Desert, David M. Miller, Kevin M. Schmidt, Shannon A. Mahan, John P. Mcgeehin, Lewis A. Owen, John A. Barron, Frank Lehmkuhl, Rene Lohrer

USGS Staff -- Published Research

New optically stimulated and radiocarbon ages for alluvial fan and lake deposits in the Mojave Desert are presented, which greatly improves the temporal resolution of surface processes. The new Mojave Desert climate-landscape record is particularly detailed for the late Holocene. Evidence from ephemeral lake deposits and landforms indicates times of sustained stream flow during a wet interval of the latter part of the Medieval Warm Period at ca. AD 1290 and during the Little Ice Age at ca. AD 1650. The former lakes postdate megadroughts of the Medieval Warm Period, whereas the latter match the Maunder Minimum of the Little ...


Enhanced Late Holocene Enso/Pdo Expression Along The Margins Of The Eastern North Pacific, John A. Barron, Lesleigh Anderson 2010 USGS

Enhanced Late Holocene Enso/Pdo Expression Along The Margins Of The Eastern North Pacific, John A. Barron, Lesleigh Anderson

USGS Staff -- Published Research

Pacific climate is known to have varied during the Holocene, but spatial patterns remain poorly defined. This paper compiles terrestrial and marine proxy data from sites along the northeastern Pacific margins and proposes that they indicate 1) suppressed ENSO conditions during the middle Holocene between ~8000 and 4000 cal BP with a North Pacific that generally resembled a La Niña-like or more negative PDO phase and 2) a climate transition between ~4200 and 3000 cal BP that appears to be the teleconnected expression to a more modern-like ENSO Pacific. Compared to modern day conditions, the compiled data suggest that during ...


Abandoned Mine Drainage In The Swatara Creek Basin, Southern Anthracite Coalfield, Pennsylvania, Usa: 2. Performance Of Treatment Systems, Charles A. Cravotta III 2010 US Geological Survey, PA Water Science Center, 215 Limekiln Rd, New Cumberland, PA, USA

Abandoned Mine Drainage In The Swatara Creek Basin, Southern Anthracite Coalfield, Pennsylvania, Usa: 2. Performance Of Treatment Systems, Charles A. Cravotta Iii

USGS Staff -- Published Research

A variety of passive and semi-passive treatment systems were constructed by state and local agencies to neutralize acidic mine drainage (AMD) and reduce the transport of dissolved metals in the upper Swatara Creek Basin in the Southern Anthracite Coalfield in eastern Pennsylvania. To evaluate the effectiveness of selected treatment systems installed during 1995–2001, the US Geological Survey collected water-quality data at upstream and downstream locations relative to each system eight or more times annually for a minimum of 3 years at each site during 1996– 2007. Performance was normalized among treatment types by dividing the acid load removed by ...


Hydrogeophysical Methods For Analyzing Aquifer Storage And Recovery Systems, Burke J. Minsley, Jonathan Ajo-Franklin, Amitabha Mukhopadhyay, Frank Dale Morgan 2010 U.S. Geological Survey

Hydrogeophysical Methods For Analyzing Aquifer Storage And Recovery Systems, Burke J. Minsley, Jonathan Ajo-Franklin, Amitabha Mukhopadhyay, Frank Dale Morgan

USGS Staff -- Published Research

Hydrogeophysical methods are presented that support the siting and monitoring of aquifer storage and recovery (ASR) systems. These methods are presented as numerical simulations in the context of a proposed ASR experiment in Kuwait, although the techniques are applicable to numerous ASR projects. Bulk geophysical properties are calculated directly from ASR flow and solute transport simulations using standard petrophysical relationships and are used to simulate the dynamic geophysical response to ASR. This strategy provides a quantitative framework for determining site-specific geophysical methods and data acquisition geometries that can provide the most useful information about the ASR implementation. An axisymmetric, coupled ...


Mercury Concentrations In Fish From A Sierra Nevada Foothill Reservoir Located Downstream From Historic Gold-Mining Operations, Michael K. Saiki, Barbara A. Martin, Thomas W. May, Charles N. Alpers 2010 U.S. Geological Survey

Mercury Concentrations In Fish From A Sierra Nevada Foothill Reservoir Located Downstream From Historic Gold-Mining Operations, Michael K. Saiki, Barbara A. Martin, Thomas W. May, Charles N. Alpers

USGS Staff -- Published Research

This study examined mercury concentrations in whole fish from Camp Far West Reservoir, an 830-ha reservoir in northern California, USA, located downstream from lands mined for gold during and following the Gold Rush of 1848–1864. Total mercury (reported as dry weight concentrations) was highest in spotted bass (mean, 0.93 μg/g; range, 0.16–4.41 μg/g) and lower in bluegill (mean, 0.45 μg/g; range, 0.22– 1.96 μg/g) and threadfin shad (0.44 μg/g; range, 0.21–1.34 μg/g). Spatial patterns for mercury in fish indicated high concentrations upstream ...


Abandoned Mine Drainage In The Swatara Creek Basin, Southern Anthracite Coalfield, Pennsylvania, Usa: 1. Stream Water Quality Trends Coinciding With The Return Of Fish, Charles A. Cravotta III, Robin A. Brightbill, Michael J. Langland 2010 US Geological Survey, PA Water Science Center, 215 Limekiln Rd, New Cumberland, PA, USA

Abandoned Mine Drainage In The Swatara Creek Basin, Southern Anthracite Coalfield, Pennsylvania, Usa: 1. Stream Water Quality Trends Coinciding With The Return Of Fish, Charles A. Cravotta Iii, Robin A. Brightbill, Michael J. Langland

USGS Staff -- Published Research

Acidic mine drainage (AMD) from legacy anthracite mines has contaminated Swatara Creek in eastern Pennsylvania. Intermittently collected base-flow data for 1959–1986 indicate that fish were absent immediately downstream from the mined area where pH ranged from 3.5 to 7.2 and concentrations of sulfate, dissolved iron, and dissolved aluminum were as high as 250, 2.0, and 4.7 mg/L, respectively. However, in the 1990s, fish returned to upper Swatara Creek, coinciding with the implementation of AMD treatment (limestone drains, limestone diversion wells, limestone sand, constructed wetlands) in the watershed. During 1996–2006, as many as 25 ...


In-Situ Stress And Fracture Characterization For Planning Of An Egs Stimulation In The Desert Peak Geothermal Field, Nevada, Stephen H. Hickman, Nicholas C. Davatzes 2010 U.S. Geological Survey

In-Situ Stress And Fracture Characterization For Planning Of An Egs Stimulation In The Desert Peak Geothermal Field, Nevada, Stephen H. Hickman, Nicholas C. Davatzes

USGS Staff -- Published Research

An integrated study of natural fracture geometry, fluid flow and stress was conducted in Desert Peak well 27-15 in preparation for development of an Enhanced Geothermal System (EGS) through hydraulic stimulation. This stimulation will be carried out at depths of ~3000 to 3500 ft in units comprised of silicified rhyolite tuffs and metamorphosed mudstones at ambient temperatures of ~180 to 195° C. Our previous analyses of borehole image logs from this well showed that the current minimum horizontal principal stress, Shmin, is oriented 114 ± 17º and that numerous fractures in the planned stimulation interval are optimally oriented for normal faulting ...


The Ties That Bind: Soil Surveyor William Edgar Tharp And Oceanographic Cartographer Marie Tharp, Edward R. Landa 2010 U.S. Geological Survey

The Ties That Bind: Soil Surveyor William Edgar Tharp And Oceanographic Cartographer Marie Tharp, Edward R. Landa

USGS Staff -- Published Research

The link between soil science and geology is personified in the American father and daughter: soil surveyor William Edgar Tharp (1870–1959) and oceanographic cartographer Marie Tharp (1920–2006). From 1904 to 1935, W.E. Tharp mapped soils in 14 states for the US Department of Agriculture, and campaigned during the late 1920s–early 1930s to raise awareness of the high rates of soil erosion from croplands. The lifestyle of the federal soil surveyor in the United States during the early 20th century involved frequent household moves, and it played a formative role in Marie Tharp’s childhood. Her path ...


Theoretical Study Of The Pyrolysis Of Methyltrichlorosilane In The Gas Phase. 3. Reaction Rate Constant Calculations, Yingbin Ge, Mark S. Gordon, Francine Battaglia, Rodney O. Fox 2010 Central Washington University

Theoretical Study Of The Pyrolysis Of Methyltrichlorosilane In The Gas Phase. 3. Reaction Rate Constant Calculations, Yingbin Ge, Mark S. Gordon, Francine Battaglia, Rodney O. Fox

Chemical and Biological Engineering Publications

The rate constants for the gas-phase reactions in the silicon carbide chemical vapor deposition of methyltrichlorosilane (Ge, Y. B.; Gordon, M. S.; Battaglia, F.; Fox, R. O. J. Phys. Chem. A 2007, 111, 1462.) were calculated. Transition state theory was applied to the reactions with a well-defined transition state; canonical variational transition state theory was applied to the barrierless reactions by finding the generalized transition state with the maximum Gibbs free energy along the reaction path. Geometry optimizations were carried out with second-order perturbation theory (MP2) and the cc-pVDZ basis set. The partition functions were calculated within the harmonic oscillator ...


An Extended Data Mining Method For Identifying Differentially Expressed Assay-Specific Signatures In Functional Genomic Studies, Derrick K. Rollins Sr., Ai-ling Teh 2010 Iowa State University

An Extended Data Mining Method For Identifying Differentially Expressed Assay-Specific Signatures In Functional Genomic Studies, Derrick K. Rollins Sr., Ai-Ling Teh

Chemical and Biological Engineering Publications

Background: Microarray data sets provide relative expression levels for thousands of genes for a small number, in comparison, of different experimental conditions called assays. Data mining techniques are used to extract specific information of genes as they relate to the assays. The multivariate statistical technique of principal component analysis (PCA) has proven useful in providing effective data mining methods. This article extends the PCA approach of Rollins et al. to the development of ranking genes of microarray data sets that express most differently between two biologically different grouping of assays. This method is evaluated on real and simulated data and ...


Immobilization Of Motile Bacterial Cells Via Dip-Pen Nanolithography, Dorjderem Nyamjav, Sergey Rozhok, Richard C. Holz 2010 Loyola University Chicago

Immobilization Of Motile Bacterial Cells Via Dip-Pen Nanolithography, Dorjderem Nyamjav, Sergey Rozhok, Richard C. Holz

Chemistry Faculty Research and Publications

A strategy to bind bacterial cells to surfaces in a directed fashion via dip-pen nanolithography (DPN) is presented. Cellular attachment to pre-designed DPN generated microarrays was found to be dependent on the shape and size of the surface feature. While this observation is likely due in part to a dense, well formed mercaptohexadecanoic acid (MHA) monolayer generated via DPN, it may also simply be due to the physical shape of the surface structure. Motile Pseudomonas aeruginosa bacterial cells were observed to bind to DPN generated mercaptohexadecanoic acid/poly-L-lysine (MHA/PLL) line patterns, 'blocks' made up of eight lines with 100 ...


Affinity Capillary Electrophoretic Study Of K+/Na+ Selectivity Of Hexaarylbenzene-Based Polyaromatic Receptor, Sille Ehala, Rajendra Rathore, Emanuel Makrlik, Petr Toman, Václav Kašička 2010 Academy of Sciences of the Czech Republic

Affinity Capillary Electrophoretic Study Of K+/Na+ Selectivity Of Hexaarylbenzene-Based Polyaromatic Receptor, Sille Ehala, Rajendra Rathore, Emanuel Makrlik, Petr Toman, Václav Kašička

Chemistry Faculty Research and Publications

Affinity capillary electrophoretic (ACE) study has proved the selectivity of hexaarylbenzene-based polyaromatic receptor (R) for K+ ion over Na+ ion. The apparent binding constants of the R complexes with K+ and Na+ ions were determined from the dependence of effective electrophoretic mobility of R on the concentration of the above alkali metal ions in the background electrolyte using a non-linear regression analysis. The apparent binding constants (Kb) of the K-R+ and Na–R+ complexes in methanolic medium were evaluated as log Kb = 3.20 ± 0.22 for the K–R+ complex, and log Kb≅−0.7 for ...


Absorption And Biotransformation Of Polybrominated Diphenyl Ethers De-71 And De-79 In Chicken (Gallus Gallus), Mallard (Anas Platyrhynchos), American Kestrel (Falco Sparverius) And Black-Crowned Night-Heron (Nycticorax Nycticorax) Eggs, Moira A. McKernan, Barnett A. Rattner, Jeff S. Hatfield, Robert C. Hale, Mary Ann Ottinger 2010 University of Maryland

Absorption And Biotransformation Of Polybrominated Diphenyl Ethers De-71 And De-79 In Chicken (Gallus Gallus), Mallard (Anas Platyrhynchos), American Kestrel (Falco Sparverius) And Black-Crowned Night-Heron (Nycticorax Nycticorax) Eggs, Moira A. Mckernan, Barnett A. Rattner, Jeff S. Hatfield, Robert C. Hale, Mary Ann Ottinger

USGS Staff -- Published Research

We recently reported that air cell administration of penta-brominated diphenyl ether (penta-BDE; DE-71) evokes biochemical and immunologic effects in chicken (Gallus gallus) embryos at very low doses, and impairs pipping (i.e., stage immediately prior to hatching) and hatching success at 1.8 µg g-1 egg (actual dose absorbed) in American kestrels (Falco sparverius). In the present study, absorption of polybrominated diphenyl ether (PBDE) congeners was measured following air cell administration of a penta-BDE mixture (11.1 lg DE-71 g-1 egg) or an octa-brominated diphenyl ether mixture (octa BDE; DE-79; 15.4 lg DE-79 g-1 egg). Uptake ...


Characterization And Correction Of Dark Current In Compact Consumer Cameras, Justin Charles Dunlap, Erik Bodegom, Ralf Widenhorn 2010 Portland State University

Characterization And Correction Of Dark Current In Compact Consumer Cameras, Justin Charles Dunlap, Erik Bodegom, Ralf Widenhorn

Physics Faculty Publications and Presentations

A study of dark current in digital imagers within consumer grade digital cameras is presented. Dark current is shown to vary with temperature, exposure time, and ISO setting. Further, dark current is shown to increase in successive images during a series of images. Consumer cameras are often designed to be as compact as possible and therefore the digital imagers within the camera frame are prone to heat generated by nearby elements within the camera body. It is the scope of this work to characterize the dark current in such cameras and to show that the dark current, in part due ...


Finite Element Analysis Of Ring-Shaped Emission Profile In Plasma Bullet, Yukinori Sakiyama, David B. Graves, Julien Jarrige, Mounir Laroussi 2010 Old Dominion University

Finite Element Analysis Of Ring-Shaped Emission Profile In Plasma Bullet, Yukinori Sakiyama, David B. Graves, Julien Jarrige, Mounir Laroussi

Electrical & Computer Engineering Faculty Publications

Using a one-way coupled model of neutral gas flow and plasma dynamics we report a mechanism to explain the ring-shaped emission pattern that has been observed experimentally in plasma bullets at atmospheric pressure. We solve a fluid model with the local field approximation in one-dimensional cylindrical coordinates, corresponding to a cross-section of a plasma bullet. Pulselike uniform electric field is assumed to be applied perpendicular to the simulation domain. Time and spatially resolved spectroscopic measurements support the simulation results.


Experimental Studies On The Plasma Bullet Propagation And Its Inhibition, Erdinc Karakas, Mounir Laroussi 2010 Old Dominion University

Experimental Studies On The Plasma Bullet Propagation And Its Inhibition, Erdinc Karakas, Mounir Laroussi

Electrical & Computer Engineering Faculty Publications

Plasma bullets generated by atmospheric pressure low temperature plasma jets have recently been an active research topic due to their unique properties and their enhanced plasma chemistry. In this paper, experimental insights into the plasma bullet lifetime and its velocity are reported. Data obtained from intensified charge-coupled device camera and time-resolved optical emission spectroscopy (OES) elucidated the existence of a weakly ionized channel between the plasma bullet and its source (such as the plasma pencil). Factors responsible for the inhibition of the propagation of the bullet, such as low helium mole fraction, the magnitude of the applied voltage, and the ...


Matrix Isolation And Computational Study Of Isodifluorodibromomethane (F2 Cbr-Br): A Route To Br2 Formation In Cf2 Br2 Photolysis, Alexander N. Tarnovsky, Lisa George, Aimable Kalume, Patrick Z. El-Khoury, Scott A. Reid 2010 Bowling Green State University

Matrix Isolation And Computational Study Of Isodifluorodibromomethane (F2 Cbr-Br): A Route To Br2 Formation In Cf2 Br2 Photolysis, Alexander N. Tarnovsky, Lisa George, Aimable Kalume, Patrick Z. El-Khoury, Scott A. Reid

Chemistry Faculty Publications

The photolysis products of dibromodifluoromethane (CF2 Br 2) were characterized by matrix isolation infrared and UV/Visible spectroscopy, supported by ab initio calculations. Photolysis at wavelengths of 240 and 266 nm of CF2 Br2:Ar samples (∼1:5000) held at ∼5 K yielded iso- CF2 Br2 (F2 CBrBr), a weakly bound isomer of CF2 Br2, which is characterized here for the first time. The observed infrared and UV/Visible absorptions of iso- CF2 Br2 are in excellent agreement with computational predictions at the B3LYP/aug-cc-pVTZ level. Single point energy calculations at the CCSD(T)/aug-cc-pVDZ level on the B3LYP optimized ...


Energy Barrier, Charge Carrier Balance, And Performance Improvement In Organic Light-Emitting Diodes, Pavel Anzenbacher Jr., Amare Benor, Shin-ya Takizawa, C. Pérez-Bolivar 2010 Bowling Green State University

Energy Barrier, Charge Carrier Balance, And Performance Improvement In Organic Light-Emitting Diodes, Pavel Anzenbacher Jr., Amare Benor, Shin-Ya Takizawa, C. Pérez-Bolivar

Chemistry Faculty Publications

The charge injection properties of poly(3,4-ethylenedioxythiophene): polystyrene sulfonate anodes are crucial for performance of organic photovoltaics and organic light-emitting diodes (OLEDs). A simple method for tuning hole injection efficiency using UV-ozone is shown to change anode work-function and optimized carriers balance in the devices and improved efficiency in OLEDs. The optimum time of treatment and work-function differs with device architecture. © 2010 American Institute of Physics.


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