Evaluation Of Oral Itraconazole Administration In Captive Humboldt Penguins (Spheniscus Humboldti), 2011 University of Tennessee, Knoxville
Evaluation Of Oral Itraconazole Administration In Captive Humboldt Penguins (Spheniscus Humboldti), Elizabeth Bunting, Noha Madi, Sherry Cox, Tomas Martin-Jimenez, Henry Fox, George Kollias
Aspergillus spp. fungal infections are the most common cause of morbidity and mortality in captive penguins. Itraconazole has been the drug of choice for both therapeutic and prophylactic treatment; however, the pharmacokinetic and pharmacodynamic parameters can be highly variable in different species, and it has not been evaluated in penguins. In this study, four preliminary steady-state trials were performed to compare two oral formulations of itraconazole (commercial capsules compared with generic bulk compounded powder) at two different dosages (6 or 12 mg/kg once a day) administered in fish to small groups of captive Humboldt penguins (Spheniscus humboldti). Building on ...
Functional Characterization Of A Lipoprotein-Encoding Operon In Campylobacter Jejuni, 2011 Iowa State University
Functional Characterization Of A Lipoprotein-Encoding Operon In Campylobacter Jejuni, Mayumi Oakland, Byeonghwa Jeon, Orhan Sahin, Zhangqi Shen, Qijing Zhang
Veterinary Microbiology and Preventive Medicine Publications
Bacterial lipoproteins have important functions in bacterial pathogenesis and physiology. In Campylobacter jejuni, a major foodborne pathogen causing gastroenteritis in humans, the majority of lipoproteins have not been functionally characterized. Previously, we showed by DNA microarray that CmeR, a transcriptional regulator repressing the expression of the multidrug efflux pump CmeABC, modulates the expression of a three-gene operon (cj0089, cj0090, and cj0091) encoding a cluster of lipoproteins in C. jejuni.
In this work, we characterized the function and regulation of the cj0089-cj0090-cj0091 operon. In contrast to the repression of cmeABC, CmeR activates the expression of the lipoprotein ...
A Prevalence Study Of Southeast Origin Sale Barn Beef Cattle, Comingled In Warren County, Kentucky, Persistently Infected With Bovine Viral Diarrhea Virus, Including The Effects Of Season And Body Weight, 2011 Western Kentucky University
A Prevalence Study Of Southeast Origin Sale Barn Beef Cattle, Comingled In Warren County, Kentucky, Persistently Infected With Bovine Viral Diarrhea Virus, Including The Effects Of Season And Body Weight, Sarah Elizabeth Thomas
Masters Theses & Specialist Projects
Bovine viral diarrhea (BVD) is an economically important disease of cattle. Calves persistently infected (PI) with the bovine viral diarrhea virus (BVDV) are a powerful agent for spread of the virus. A total of 24,423 southeast origin beef cattle comingled at three Warren County, Kentucky locations were tested from November 2007 to June 2010 for PI BVDV. A total of 97 head tested positive for PI BVDV, giving an average overall prevalence of 0.397%.
Calves tested were subdivided into categories for additional calculations of dependence. A total of 8,910 were categorized by weight range upon testing (300-399 ...
Three Rs Approaches In The Production And Quality Control Of Fish Vaccines, 2011 Center for Veterinary Biologics
Three Rs Approaches In The Production And Quality Control Of Fish Vaccines, Paul J. Midtlyng, Coenraad F.M. Hendriksen, Elisabeth Balks, Lukas Bruckner, Lawrence Elsken, Øystein Evensen, Kjetil Fyrand, Allison Guy, Marlies Halder, Penny Hawkins, Gunn Kisen, Anne Berit Romstad, Kira Salonius, Patrick Smith, Lynne U. Sneddon
VETERINARY SCIENCE AND MEDICINE
The workshop on Three Rs Approaches in the Production and Quality Control of Fish Vaccines aimed a) to identify animal tests currently stipulated for the production and quality control of fish vaccines and to highlight animal welfare concerns associated with these tests; b) to identify viable options to replace, reduce, and refine animal use for fish vaccine testing; and c) to discuss the way forward and set out how the Three Rs may be implemented without jeopardizing the quality of the vaccines. The workshop participants -- experts from academia, regulatory authorities, a scientific animal welfare organization, and the fish vaccine industry ...
Pharmacokinetics Of Oral Tramadol In Red-Tailed Hawks (Buteo Jamaicensis), 2011 University of Tennessee - Knoxville
Pharmacokinetics Of Oral Tramadol In Red-Tailed Hawks (Buteo Jamaicensis), Marcy Souza, Tomas Martin-Jimenez, Michael Jones, Sherry Cox
No abstract provided.
Clinical And Immunomodulating Effects Of Ketamine In Horses With Experimental Endotoxemia, 2011 Iowa State University
Clinical And Immunomodulating Effects Of Ketamine In Horses With Experimental Endotoxemia, Cody J. Alcott, Brett A. Sponseller, David M. Wong, J. L. Davis, A. M. Soliman, Chong Wang, Walter H. Hsu
Veterinary Diagnostic and Production Animal Medicine Publications
Background: Ketamine has immunomodulating effects both in vitro and in vivo during experimental endotoxemia in humans, rodents, and dogs.
Hypothesis: Subanesthetic doses of ketamine will attenuate the clinical and immunologic responses to experimental endotoxemia in horses.
Animals: Nineteen healthy mares of various breeds.
Methods: Experimental study. Horses were randomized into 2 groups: ketamine-treated horses (KET; n = 9) and saline-treated horses (SAL; n = 10). Both groups received 30 ng/kg of lipopolysaccharide (LPS, Escherichia coli, O55:B5) 1 hour after the start of a continuous rate infusion (CRI) of racemic ketamine (KET) or physiologic saline (SAL). Clinical and hematological responses were ...
Sensitivity And Specificity Of A Nested Polymerase Chain Reaction For Detection Of Lentivirus Infection In Lions (Panthera Leo), 2010 University of Tennessee - Knoxville
Sensitivity And Specificity Of A Nested Polymerase Chain Reaction For Detection Of Lentivirus Infection In Lions (Panthera Leo), H Adams, M Van Vuuren, Stephen Kania, A Bosman, D Keet, J New, Melissa Kennedy
Melissa A. Kennedy
Feline immunodeficiency virus (FIV) is a lentivirus in the Retroviridae family that causes lifelong infection in domestic cats. The lentivirus of African lions (Panthera leo), referred to as FIVple, is endemic in certain lion populations in eastern and southern Africa. Lentivirus infection leads to immunologic dysfunction and immunosuppressive disease in domestic cats; however, little is known about the pathogenic effects of infection in lions, nor about the epidemiologic impact on free-ranging and captive populations. Whole blood and serum samples were collected opportunistically from free-ranging lions in Kruger National Park, Republic of South Africa (RSA). Whole blood and serum samples were ...
Determination Of Carboplatin In Canine Plasma By High-Performance Liquid Chromatography, 2010 University of Tennessee - Knoxville
Determination Of Carboplatin In Canine Plasma By High-Performance Liquid Chromatography, Nicolas Villarino, Sherry Cox, Jason Yarbrough, Tomas Martin-Jimenez
Carboplatin is an antineoplastic drug administered to treat different tumoral conditions in canine oncology. The objective of this study was to validate a high-performance chromatographic (HPLC) method which could be applied in canine pharmacokinetic studies. Following ultrafiltration using a Centrifree device, standards, quality controls and plasma samples were separated by isocratic reversed-phase HPLC on an Inertsil ODS-2 (250 × 4.6 mm i.d.) analytical column and quantified using UV detection at 220 nm. The mobile phase was potassium phosphate (pH 4.5), with a flow-rate of 1.0 mL/min. The procedure produced a linear curve (r2 > 0.999) over ...
Mechanisms Of The Extreme Sensitivity Of Turkeys To Aflatoxin B1, 2010 Utah State University
Mechanisms Of The Extreme Sensitivity Of Turkeys To Aflatoxin B1, Sumit Rawal
All Graduate Theses and Dissertations
The pathogenesis of hepatotoxic and hepatocarcinogenic actions of the mycotoxin aflatoxin B1 (AFB1) involves initial bioactivation by microsomal cytochrome P450s (P450) to a reactive and electrophilic intermediate, exo-aflatoxin B1-8,9-epoxide (exo-AFBO). Poultry, especially turkeys, are extremely sensitive to AFB1, a condition associated with efficient epoxidation by P450s. The purpose of this research was to 1) discover and characterize the P450s in turkey liver responsible for AFB1 bioactivation, and 2) determine the relative importance of these P450s in turkey liver. Initial investigations led to the discovery of CYP1A5. We then identified CYP3A37, a human CYP3A4 homologue from turkey liver, which along ...
Measurement Of C-Peptide Concentrations And Responses To Somatostatin, Glucose Infusion, And Insulin Resistance In Horses, 2010 University of Tennessee - Knoxville
Measurement Of C-Peptide Concentrations And Responses To Somatostatin, Glucose Infusion, And Insulin Resistance In Horses, F. Toth, N. Frank, Tomas Martin-Jimenez, S. Elliott, R. Geor, R. Boston
Reasons for performing study: Hyperinsulinaemia is detected in horses with insulin resistance (IR) and has previously been attributed to increased pancreatic insulin secretion. Connecting peptide (C-peptide) can be measured to assess pancreatic function because it is secreted in equimolar amounts with insulin and does not undergo hepatic clearance. Hypothesis: A human double antibody radioimmunoassay (RIA) detects C-peptide in equine serum and concentrations would reflect responses to different stimuli and conditions. Methods: A validation procedure was performed to assess the RIA. Six mature mares were selected and somatostatin administered i.v. as a primed continuous rate infusion, followed by 50 nmol ...
Serum Concentrations Of Methimazole In Cats After A Single Oral Dose Of Controlled-Release Carbimazole Or Sugar-Coated Methimazole (Thiamazole)., 2009 University of Tennessee, Knoxville
Serum Concentrations Of Methimazole In Cats After A Single Oral Dose Of Controlled-Release Carbimazole Or Sugar-Coated Methimazole (Thiamazole)., S. Longhofer, Tomas Martin-Jimenez, J. Soni-Gupta
No abstract provided.