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379 full-text articles. Page 2 of 7.

In Vitro Cytotoxicity And Pharmacokinetic Evaluation Of Pharmacological Ascorbate In Dogs, Margaret L. Musser, Alyssa L. Mahaffey, Melissa A. Fath, Garry R. Buettner, Brett A. Wagner, Benjamin K. Schneider, Yeon-Jung Seo, Jonathan P. Mochel, Chad M. Johannes 2019 Iowa State University

In Vitro Cytotoxicity And Pharmacokinetic Evaluation Of Pharmacological Ascorbate In Dogs, Margaret L. Musser, Alyssa L. Mahaffey, Melissa A. Fath, Garry R. Buettner, Brett A. Wagner, Benjamin K. Schneider, Yeon-Jung Seo, Jonathan P. Mochel, Chad M. Johannes

Biomedical Sciences Publications

Background: High-dose, pharmacological ascorbate (P-AscH−) is preferentially cytotoxic to human cancer cells in vitro. Investigations on the efficacy of P-AscH− as an adjuvant treatment for multiple human cancers are on-going, but has yet to be formally investigated in dogs. The primary objectives of this study were to determine the pharmacokinetic (PK) profile of P-AscH− in healthy Beagle dogs and the effects of P-AscH− on canine osteosarcoma cells in vitro.

Methods: Eight purpose-bred, healthy, spayed female Beagle dogs, between 20 and 21 months old, and 8–10 kg were administered two doses of P-AscH− (550 or 2,200 mg/kg) via ...


Antibodies Against Actinobacillus Pleuropneumoniae, Mycoplasma Hyopneumoniae And Influenza Virus And Their Relationships With Risk Factors, Clinical Signs And Lung Lesions In Pig Farms With One-Site Production Systems In Brazil, Thaís G. Baraldi, Nathan R.N. Cruz, Daniele A. Pereira, J. V.B. Galdeano, Igor R.H. Gatto, A. F.D. Silva, Andrea Panzardi, Daniel C.L. Linhares, Luis A. Mathias, Luís G. de Oliveira 2019 São Paulo State University (Unesp)

Antibodies Against Actinobacillus Pleuropneumoniae, Mycoplasma Hyopneumoniae And Influenza Virus And Their Relationships With Risk Factors, Clinical Signs And Lung Lesions In Pig Farms With One-Site Production Systems In Brazil, Thaís G. Baraldi, Nathan R.N. Cruz, Daniele A. Pereira, J. V.B. Galdeano, Igor R.H. Gatto, A. F.D. Silva, Andrea Panzardi, Daniel C.L. Linhares, Luis A. Mathias, Luís G. De Oliveira

Veterinary Diagnostic and Production Animal Medicine Publications

A study was conducted on 21 pig herds using one-site production system in the southeast region of Brazil to assess the relationships among serological results for primary pathogens involved in respiratory diseases (Actinobacillus pleuropneumoniae, App; Mycoplasma hyopneumoniae, Mhyo; and swine influenza virus, SIV), cough index, pneumonia index, pleuritis and herd characteristics. The prevalence of antibodies against Mhyo and SIV increased throughout the raising phases, with the highest prevalence in slaughtered pigs (> 40%), while pigs in 65% (14/21) of nurseries demonstrated marked seroprevalence of App that decreased until the day of slaughter. Pleuritis and pulmonary consolidations were recorded in 9 ...


North American Hard Yellow Liver Disease: An Old Problem Readdressed, Bryan L. Stegelmeier, Meredyth Jones, Christopher P. Womack, T Zane Davis, Dale R. Gardner 2019 USDA-Agricultural Research Service

North American Hard Yellow Liver Disease: An Old Problem Readdressed, Bryan L. Stegelmeier, Meredyth Jones, Christopher P. Womack, T Zane Davis, Dale R. Gardner

Poisonous Plant Research (PPR)

Hard yellow liver disease or fatty cirrhosis periodically affects cattle, sheep, goats, pronghorn antelope (Antilocapra americana) and whitetail deer (Odocoileus virginianus texanus) within several Texas counties in the United States. Clinically it presents as chronic liver disease with progressive hepatic necrosis and fibrosis, icterus and liver failure. The damaged livers are yellow and many have multiple firm, often gritty foci that are scattered throughout all lobes. Early investigations included feeding studies using potential toxic plants, climate and forage studies, infectious disease surveys and various mycotoxin studies and analyses. None have definitively reproduced the disease or identified the inciting cause. However ...


Physiological And Behavioural Evaluation Of Common Anaesthesia Practices In The Rainbow Trout, Kieran C. Pounder, Jennifer L. Mitchell, Jack S. Thomson, Tom G. Pottinger, Lynne U. Sneddon 2019 University of Liverpool

Physiological And Behavioural Evaluation Of Common Anaesthesia Practices In The Rainbow Trout, Kieran C. Pounder, Jennifer L. Mitchell, Jack S. Thomson, Tom G. Pottinger, Lynne U. Sneddon

Lynne Sneddon, PhD

Anaesthetic drugs are commonly administered to fish in aquaculture, research and veterinary contexts. Anaesthesia causes temporary absence of consciousness and may reduce the stress and/or pain associated with handling and certain invasive procedures. The rainbow trout (Oncorhynchus mykiss) is a widely-used model species with relevance to both aquaculture and natural ecosystems. This study sought to establish the relative acute impact of commonly used anaesthetics on rainbow trout when used for anaesthesia or euthanasia by exploring their effects on aversion behaviour and stress physiology. Five widely used anaesthetics were investigated at two concentrations reflective of common laboratory practises: MS-222, benzocaine ...


Reduction In Activity By Noxious Chemical Stimulation Is Ameliorated By Immersion In Analgesic Drugs In Zebrafish, Javier Lopez-Luna, Qussay Al-Jubouri, Waleed Al-Nuaimy, Lynne U. Sneddon 2019 University of Liverpool

Reduction In Activity By Noxious Chemical Stimulation Is Ameliorated By Immersion In Analgesic Drugs In Zebrafish, Javier Lopez-Luna, Qussay Al-Jubouri, Waleed Al-Nuaimy, Lynne U. Sneddon

Lynne Sneddon, PhD

Research has recently demonstrated that larval zebrafish show similar molecular responses to nociception to those of adults. Our study explored whether unprotected larval zebrafish exhibited altered behaviour after exposure to noxious chemicals and screened a range of analgesic drugs to determine their efficacy to reduce these responses. This approach aimed to validate larval zebrafish as a reliable replacement for adults as well as providing a high-throughput means of analysing behavioural responses. Zebrafish at 5 days postfertilization were exposed to known noxious stimuli: acetic acid (0.01%, 0.1% and 0.25%) and citric acid (0.1%, 1% and 5%). The ...


Evidence Of Improved Reporting Of Swine Vaccination Trials In The Post-Reflect Statement Publication Period, Cesar A. A. Moura, Sarah Totton, Jan M. Sargeant, Terri L. O’Sullivan, Daniel C. L. Linhares, Annette M. O'Connor 2019 Iowa State University

Evidence Of Improved Reporting Of Swine Vaccination Trials In The Post-Reflect Statement Publication Period, Cesar A. A. Moura, Sarah Totton, Jan M. Sargeant, Terri L. O’Sullivan, Daniel C. L. Linhares, Annette M. O'Connor

Veterinary Diagnostic and Production Animal Medicine Publications

Objectives: Describe and compare the proportion of studies reporting the method used to assign study units to treatment groups, reporting a random allocation approach, reporting 18 REFLECT items, and the proportion of studies having a low risk-of-bias assessment in swine vaccination trial studies published after the REFLECT statement, compared to studies published before.

Materials and Methods: The study population was 61 studies that evaluated vaccines targeted at pathogens affecting swine health or pork safety. Two reviewers assessed the reporting of 18 of 22 REFLECT items and 5 risk-of-bias domains.

Results: Authors reported the method used to allocate experimental units in ...


Fluorophotometric Assessment Of Tear Volume And Turnover Rate In Healthy Dogs And Cats, Lionel Sebbag, Rachel A. Allbaugh, Rita F. Wehrman, Lisa Uhl, Gil Ben-Shlomo, Thomas Chen, Jonathan P. Mochel 2019 Iowa State University

Fluorophotometric Assessment Of Tear Volume And Turnover Rate In Healthy Dogs And Cats, Lionel Sebbag, Rachel A. Allbaugh, Rita F. Wehrman, Lisa Uhl, Gil Ben-Shlomo, Thomas Chen, Jonathan P. Mochel

Veterinary Clinical Sciences Publications

Purpose: The study establishes normative data of tear volume (TV) and tear turnover rate (TTR) in healthy dogs and cats, 2 species commonly used for translational research in ophthalmology.

Methods: Thirty-six dogs and 24 cats were enrolled, encompassing a variety of breeds with diverse skull conformations (brachycephalic, mesocephalic, and dolichocephalic). Two microliters of 10% fluorescein were instilled onto the upper bulbar conjunctiva of both eyes, followed by tear collection with 2-μL capillary tubes at 0, 2, 4, 6, 10, 15, and 20 min. Fluorescein concentrations were measured with a computerized scanning ocular fluorophotometer. The TV and TTR were estimated based ...


Release Kinetics Of Methylene Blue In Pluronic F-127 Hydrogel, Meredith Hayden 2019 University of Arkansas, Fayetteville

Release Kinetics Of Methylene Blue In Pluronic F-127 Hydrogel, Meredith Hayden

Animal Science Undergraduate Honors Theses

“Between January 1998 and March 2000, veterinarians at the ASPCA National Animal Poison Control Center (NAPCC) consulted on more than 1,050 cases of accidental exposures to acetaminophen and 1,100 cases of ibuprofen ingestion in dogs and cats” (Richardson, 2000).

NSAID toxicity has become an increasingly common occurrence in small companion animals within the last decade due to widespread usage (Zhou, Boudreau, & Freedman, 2014). Depending on the specific drug, treatment is centered around early decontamination and prevention of kidney and hepatic damage, and methemoglobanemia. Currently, methemoglobanemia is treated with intravenous delivery of methylene blue and less commonly through oral ...


Pharmacokinetic Modeling Of Ceftiofur Sodium Using Nonlinear Mixed-Effects In Healthy Beagle Dogs, Jianzhong Wang, Benjamin K. Schneider, Jiao Xue, Pan Sun, Jicheng Qiu, Jonathan P. Mochel, Xingyuan Cao 2019 Iowa State University and China Agricultural University

Pharmacokinetic Modeling Of Ceftiofur Sodium Using Nonlinear Mixed-Effects In Healthy Beagle Dogs, Jianzhong Wang, Benjamin K. Schneider, Jiao Xue, Pan Sun, Jicheng Qiu, Jonathan P. Mochel, Xingyuan Cao

Biomedical Sciences Publications

Ceftiofur (CEF) sodium is a third-generation broad-spectrum cephalosporin commonly used in an extra-label manner in dogs for the treatment of respiratory and urinary system infections. To contribute to the literature supporting CEF use in companion animals, we have developed a compartmental, nonlinear mixed-effects (NLME) model of CEF pharmacokinetics in dogs (PK). We then used the mathematical model to predict (via Monte Carlo simulation) the duration of time for which plasma concentrations of CEF and its pharmacologically active metabolites remained above minimum inhibitory concentrations (respiratory tract Escherichia coli spp). Twelve healthy beagle dogs were administered either 2.2 mg/kg ceftiofur-sodium ...


Histamine-Induced Conjunctivitis And Breakdown Of Blood–Tear Barrier In Dogs: A Model For Ocular Pharmacology And Therapeutics, Lionel Sebbag, Rachel A. Allbaugh, Amanda Weaver, Yeon-Jung Seo, Jonathan P. Mochel 2019 Iowa State University

Histamine-Induced Conjunctivitis And Breakdown Of Blood–Tear Barrier In Dogs: A Model For Ocular Pharmacology And Therapeutics, Lionel Sebbag, Rachel A. Allbaugh, Amanda Weaver, Yeon-Jung Seo, Jonathan P. Mochel

Biomedical Sciences Publications

Conjunctival inflammation disturbs the blood–tear barrier and thus affects the tear film stability and composition. We aimed to develop a non-invasive and reliable method to induce conjunctivitis in dogs, a large animal model for translational work on ocular surface disease in humans. Six beagle dogs underwent a randomized, vehicle-controlled, balanced crossover trial—on six separate days, one eye received topical artificial tears (vehicle), while the other eye received one of six concentrations of histamine solution (0.005–500 mg/ml). At sequential times after eyedrop administration, a conjunctivitis score was given to each eye based on the degree of ...


Pentablock Copolymer Micelle Nanoadjuvants Enhance Cytosolic Delivery Of Antigen And Improve Vaccine Efficacy While Inducing Low Inflammation, Sujata Senapati, Ross J. Darling, Darren Loh, Ian C. Schneider, Michael J. Wannemeuhler, Balaji Narasimhan, Surya K. Mallapragada 2019 Iowa State University

Pentablock Copolymer Micelle Nanoadjuvants Enhance Cytosolic Delivery Of Antigen And Improve Vaccine Efficacy While Inducing Low Inflammation, Sujata Senapati, Ross J. Darling, Darren Loh, Ian C. Schneider, Michael J. Wannemeuhler, Balaji Narasimhan, Surya K. Mallapragada

Chemistry Publications

As the focus has shifted from traditional killed or live, attenuated vaccines towards subunit vaccines, improvements in vaccine safety have been confronted with low immunogenicity of protein antigens. This issue has been addressed by synthesizing and designing a wide variety of antigen carriers and adjuvants, such as Toll-like receptor agonists (e.g., MPLA, CpG). Studies have focused on optimizing adjuvants for improved cellular trafficking, cytosolic availability, and improved antigen presentation. In this work, we describe the design of novel amphiphilic pentablock copolymer (PBC) adjuvants that exhibit high biocompatibility and reversible pH- and temperature-sensitive micelle formation. We demonstrate improved humoral immunity ...


The Role Of The Gut-Brain Axis In Neurodegenerative Diseases And Relevance Of The Canine Model: A Review, Yoko Ambrosini, Dana Borcherding, Anumantha Kanthasamy, Hyun Jung Kim, Albert Jergens, Karin Allenspach, Jonathan Mochel 2019 Iowa State University

The Role Of The Gut-Brain Axis In Neurodegenerative Diseases And Relevance Of The Canine Model: A Review, Yoko Ambrosini, Dana Borcherding, Anumantha Kanthasamy, Hyun Jung Kim, Albert Jergens, Karin Allenspach, Jonathan Mochel

Biomedical Sciences Publications

Identifying appropriate animal models is critical in developing translatable in vitro and in vivo systems for therapeutic development and investigating disease pathophysiology. These animal models should have direct biological and translational relevance to the underlying disease they are supposed to mimic. Aging dogs naturally develop a cognitive decline in many aspects including learning and memory, but also exhibit human-like individual variability in the aging process. Neurodegenerative processes that can be observed in both human and canine brains include the progressive accumulation of β-amyloid (Aβ) found as diffuse plaques in the prefrontal cortex, including the gyrus proreus, the hippocampus, and in ...


Diseases Of Swine, Jeffrey J. Zimmerman, Locke A. Karriker, Alejandro Ramirez, Kent J. Schwartz, Gregory W. Stevenson, Jianqiang Zhang 2019 Iowa State University

Diseases Of Swine, Jeffrey J. Zimmerman, Locke A. Karriker, Alejandro Ramirez, Kent J. Schwartz, Gregory W. Stevenson, Jianqiang Zhang

Veterinary Diagnostic and Production Animal Medicine Books

Provides a fully revised Eleventh Edition of the definitive reference to swine health and disease

Diseases of Swine has been the definitive reference on swine health and disease for over 60 years. This new edition has been completely revised to include the latest information, developments, and research in the field. Now with full color images throughout, this comprehensive and authoritative resource has been redesigned for improved consistency and readability, with a reorganized format for more intuitive access to information.

Diseases of Swine covers a wide range of essential topics on swine production, health, and management, with contributions from more than ...


A Review Of Relevant Therapeutic Targets For The Management Of Congestive Heart Failure In Dogs: Present And Future Directions, Jonathan P. Mochel 2019 Iowa State University

A Review Of Relevant Therapeutic Targets For The Management Of Congestive Heart Failure In Dogs: Present And Future Directions, Jonathan P. Mochel

Biomedical Sciences Publications

Congestive heart failure (CHF) is a major cause of morbidity and mortality with an increasing prevalence in human and canine populations. Similar to humans, overactivation of the renin-angiotensin aldosterone system is involved in the pathophysiology of CHF in dogs. Current therapeutic strategies for the management of canine CHF include the use of RAAS inhibitors, diuretics and inodilators. The present review summarizes data from our own research on the modulation of the renin-angiotensin cascade in dogs in dogs, together with new findings (including novel therapeutic targets) from the veterinary and the human literature.


Assessment Of Immediate Production Impact Following Attenuated Prrs Type 2 Virus Vaccination In Swine Breeding Herds, Cesar Augus Amorim Moura, Clayton Johnson, Samuel R. Baker, Derald J. Holtkamp, Chong Wang, Daniel C.L. Linhares 2019 Iowa State University

Assessment Of Immediate Production Impact Following Attenuated Prrs Type 2 Virus Vaccination In Swine Breeding Herds, Cesar Augus Amorim Moura, Clayton Johnson, Samuel R. Baker, Derald J. Holtkamp, Chong Wang, Daniel C.L. Linhares

Veterinary Diagnostic and Production Animal Medicine Publications

Background

To mitigate production impact of porcine reproductive and respiratory syndrome (PRRS) virus outbreaks, it has been common to preventively vaccinate swine breeding herds using PRRS modified live virus (MLV) vaccine. However, attenuated PRRS virus (PRRSv) may result negative impact on farm productivity. The objective of this study was to measure the immediate impact of PRRS type 2 MLV vaccine on breeding herd performance under field conditions. Eight PRRS-stable farms routinely mass vaccinating females with commercial PRRS MLV vaccines were enrolled on study. Vaccination dates were collected and weekly changes in abortions, neonatal losses, pre-weaning mortality, pigs weaned per sow ...


Praziquantel Resistance In The Zoonotic Cestode Dipylidium Caninum, Jeba Jesudoss Chelladurai, Tsegabirhan Kifleyohannes, Janelle Scott, Matthew T. Brewer 2018 Iowa State University

Praziquantel Resistance In The Zoonotic Cestode Dipylidium Caninum, Jeba Jesudoss Chelladurai, Tsegabirhan Kifleyohannes, Janelle Scott, Matthew T. Brewer

Veterinary Pathology Publications and Papers

Dipylidium caninum is a cosmopolitan cestode infecting dogs, cats, and humans. Praziquantel is a highly effective cestocidal drug and resistance in adult cestodes has not been reported. From 2016 to 2018, a population of dogs with cestode infections that could not be eliminated despite multiple treatments with praziquantel or epsiprantel was identified. Cases of D. caninum were clinically resistant to praziquantel and could not be resolved despite increasing the dose, frequency, and duration of treatment. Resistant isolates were identified and characterized by sequencing the 28S, 12S, and voltage-gated calcium channel beta subunit genes. Cases were only resolved following treatment with ...


Veterinary Considerations For The Theoretical Resurrection Of Extinct Species, K. M. Feye, Joseph S. Smith, Lionel Sebbag, A. E. Hohman, Sreemoyee Acharya, Benjamin K. Schneider, J. T. Tucker, Lucia A. Cherep, B. R. Nordeng, A. L. Richardson, Meghan C. Gage, Diou Luo, Denusha Shrestha, Patricia Izbicki, Emir Malovic, M. A. Jefferson, Sireesha Manne, Pongrat Jaisil, Naveen Kondru, Nyzil Massey, Brandon S. Klinedinst, Steve A. Carlson 2018 Iowa State University

Veterinary Considerations For The Theoretical Resurrection Of Extinct Species, K. M. Feye, Joseph S. Smith, Lionel Sebbag, A. E. Hohman, Sreemoyee Acharya, Benjamin K. Schneider, J. T. Tucker, Lucia A. Cherep, B. R. Nordeng, A. L. Richardson, Meghan C. Gage, Diou Luo, Denusha Shrestha, Patricia Izbicki, Emir Malovic, M. A. Jefferson, Sireesha Manne, Pongrat Jaisil, Naveen Kondru, Nyzil Massey, Brandon S. Klinedinst, Steve A. Carlson

Veterinary Diagnostic and Production Animal Medicine Publications

The de-extinction of the dinosaur is a dubious possibility but its consideration brings forth some issues that are at least worthy of scientific discussion. In this review, we discuss two distinct issues that have implications for a de-extinct species such as a dinosaur: the ability, or lack thereof, to safely sedate a rare and potentially fractious animal capable of harming the veterinary staff tasked with its care; and, disease risks associated with a species that has been extinct for millions of years. To identify potential sedatives, comparative pharmacology will be needed to uncover the links between receptor pharmacology and the ...


Pharmacokinetics Of Fentanyl Citrate And Norfentanyl In Holstein Calves And Effect Of Analytical Performances On Fentanyl Parameter Estimation, Joe Smith, Johann F. Coetzee, Isaac W. G. Fisher, David J. Borts, Jonathan P. Mochel 2018 Iowa State University

Pharmacokinetics Of Fentanyl Citrate And Norfentanyl In Holstein Calves And Effect Of Analytical Performances On Fentanyl Parameter Estimation, Joe Smith, Johann F. Coetzee, Isaac W. G. Fisher, David J. Borts, Jonathan P. Mochel

Veterinary Diagnostic and Production Animal Medicine Publications

This study describes the pharmacokinetics of intravenously administered (i.v.) fentanyl citrate, and its primary metabolite norfentanyl in Holstein calves. Eight calves (58.6 ± 2.2 kg), aged 3–4 weeks, were administered fentanyl citrate at a single dose of 5.0 μg/kg i.v. Blood samples were collected from 0 to 24 hr. Plasma (nor)fentanyl concentrations were determined using liquid chromatography with mass spectrometry and a lower limit of quantification (LLOQ) of 0.03 ng/ml. To explore the effect of analytical performance on fentanyl parameter estimation, the noncompartmental pharmacokinetic analysis was then repeated with a hypothetical ...


Kisspeptin Modulates Luteinizing Hormone Release And Ovarian Follicular Dynamics In Pre-Pubertal And Adult Murrah Buffaloes, Vishalkumar Pottapenjera, Srinivasa R. Rajanala, Chandrasekhar Reddy, Arunakumari Gangineni, Kiran Avula, Sandeep K. Bejjanki, Sriravali Sathagopam, Surabhi Kesharwani, Sathya Velmurugan 2018 P. V. Narsimha Rao Telangana Veterinary University, India

Kisspeptin Modulates Luteinizing Hormone Release And Ovarian Follicular Dynamics In Pre-Pubertal And Adult Murrah Buffaloes, Vishalkumar Pottapenjera, Srinivasa R. Rajanala, Chandrasekhar Reddy, Arunakumari Gangineni, Kiran Avula, Sandeep K. Bejjanki, Sriravali Sathagopam, Surabhi Kesharwani, Sathya Velmurugan

Pharmacology and Nutritional Sciences Faculty Publications

Kisspeptin is a neuropeptide that governs the reproductive axis upstream to GnRH. We wanted to study whether kisspeptin modulates plasma LH and FSH levels and ovarian follicular dynamics in buffaloes and whether kisspeptin can be used for fixed time artificial insemination (FTAI). We carried out these studies in comparison with buserelin, a potent GnRH agonist. Kisspeptin dose-dependently increased plasma LH levels. However, the kisspeptin-induced increase in LH was short-lived as the peak reached in 15–30 min returned to basal values by 1–2 h. The kisspeptin-induced increase in LH level was less compared to buserelin-induced increase in LH level ...


Impact Of Flow Rate, Collection Devices, And Extraction Methods On Tear Concentrations Following Oral Administration Of Doxycycline In Dogs And Cats, Lionel Sebbag, Lucas Showman, Emily M. McDowell, Ann Perera, Jonathan P. Mochel 2018 Iowa State University

Impact Of Flow Rate, Collection Devices, And Extraction Methods On Tear Concentrations Following Oral Administration Of Doxycycline In Dogs And Cats, Lionel Sebbag, Lucas Showman, Emily M. Mcdowell, Ann Perera, Jonathan P. Mochel

Veterinary Diagnostic and Production Animal Medicine Publications

Purpose: Compare the precision of doxycycline quantification in tear fluid collected with either Schirmer strips or polyvinyl acetal (PVA) sponges following oral drug administration.

Methods: Three dogs and 3 cats were administered doxycycline orally at a dose of 4.2–5 mg/kg every 12 h for 6 consecutive days. At day 5 and 6, blood and tear fluid were sampled to capture doxycycline trough and maximal concentrations. Tear fluid was collected 3 times (spaced 10 min apart) at each session with the absorbent material placed in the lower conjunctival fornix until the 20-mm mark was reached (Schirmer strip, one ...


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