Spatial Analysis Of Factors Influencing Long-Term Stress In The Grizzly Bear (Ursus Arctos) Population Of Alberta, Canada,
2013
University of Victoria
Spatial Analysis Of Factors Influencing Long-Term Stress In The Grizzly Bear (Ursus Arctos) Population Of Alberta, Canada, Mathieu Bourbonnais, Trisalyn Nelson, Mark Cattet, Chris T. Darimont, Gordon Stenhouse
Physiology Collection
Non-invasive measures for assessing long-term stress in free ranging mammals are an increasingly important approach for understanding physiological responses to landscape conditions. Using a spatially and temporally expansive dataset of hair cortisol concentrations (HCC) generated from a threatened grizzly bear (Ursus arctos) population in Alberta, Canada, we quantified how variables representing habitat conditions and anthropogenic disturbance impact long-term stress in grizzly bears. We characterized spatial variability in male and female HCC point data using kernel density estimation and quantified variable influence on spatial patterns of male and female HCC stress surfaces using random forests. Separate models were developed for regions …
Stress And Reproductive Hormones In Grizzly Bears Reflect Nutritional Benefits And Social Consequences Of A Salmon Foraging Niche,
2013
University of Victoria
Stress And Reproductive Hormones In Grizzly Bears Reflect Nutritional Benefits And Social Consequences Of A Salmon Foraging Niche, Heather M. Bryan, Chris T. Darimont, Paul C. Paquet, Katherine E. Wynne-Edwards, Judit E. G. Smits
Physiology Collection
Physiological indicators of social and nutritional stress can provide insight into the responses of species to changes in food availability. In coastal British Columbia, Canada, grizzly bears evolved with spawning salmon as an abundant but spatially and temporally constrained food source. Recent and dramatic declines in salmon might have negative consequences on bear health and ultimately fitness. To examine broadly the chronic endocrine effects of a salmon niche, we compared cortisol, progesterone, and testosterone levels in hair from salmon-eating bears from coastal BC (n = 75) with the levels in a reference population from interior BC lacking access to salmon …
A Free-Ranging, Feral Mare Equus Caballus Affords Similar Maternal Care To Her Genetic And Adopted Offspring,
2013
Princeton University
A Free-Ranging, Feral Mare Equus Caballus Affords Similar Maternal Care To Her Genetic And Adopted Offspring, Cassandra M.V. Nuñez, James S. Adelman, Daniel I. Rubenstein
Cassandra M.V. Nuñez
Adoption of nongenetic offspring occurs in a variety of species but is rare in equids. We report a case of adoption by a free-ranging, feral mare Equus caballus and compare the maternal care received by her genetic offspring (born 1995) to that of her adopted offspring (born 1996) for the first 30 weeks of development. We compare five measures of care: (1) total time spent suckling, (2) mare aggression during suckling, (3) number of mare-terminated suckling bouts, (4) contact maintenance, and (5) mare-foal distance. For most behaviors, we detected no difference in the mare’s treatment of the two foals; however, …
Kisspeptin Receptor Agonist (Ftm080) Increased Plasma Concentrations Of Luteinizing Hormone In Anestrous Ewes,
2013
University of Tennessee - Knoxville
Kisspeptin Receptor Agonist (Ftm080) Increased Plasma Concentrations Of Luteinizing Hormone In Anestrous Ewes, Brian K. Whitlock, Kelly A. Chameroy, Rebecca R. Payton, Shinya Oishi, Nobutaka Fujii, Joseph A. Daniel
Brian K Whitlock, PhD, DVM, DACT
Kisspeptin (KP) and the KP receptor (Kiss1r) are integral to central regulation of the gonadotropic-axis. The demonstration that intravenous infusion of KP can stimulate gonadotropin secretion and ovulation in seasonally anestrous female sheep offers a means of manipulating the reproductive axis. However, KP may be of limited clinical use because of the short circulating half-life. Kiss1r agonists with increased half-life and similar efficacy to KP in vitro potentially may provide beneficial applications in breeding management of many species. However, many of these agonists have not been tested in vivo. This study was designed to test and compare the effects of …
Effect Of Meloxicam On Gain And Inflammatory Response Of Calves Castrated By Banding Post-Weaning,
2013
University of Tennessee - Knoxville
Effect Of Meloxicam On Gain And Inflammatory Response Of Calves Castrated By Banding Post-Weaning, Brian K. Whitlock, Peter D. Krawczel, Jeff A. Carroll, Nicole C. Burdick Sanchez, Jeff W. Dailey, Joseph A. Daniel, Johann F. Coetzee
Brian K Whitlock, PhD, DVM, DACT
Castration may detrimentally affect the health and performance of weaned calves, and painful procedures are increasingly becoming a public concern. The objective of this study was to determine the effects of castration (by banding) with or without administration of meloxicam, a non-steroid anti-inflammatory, on performance and inflammatory response in weaned beef calves. Forty-eight (weaned) beef calves [10.0±0.2 mo old; 304±6 kg BW] were blocked by age, BW, wither height, scrotal circumference, and source, then randomly assigned to 1 of 3 treatments (n=16 calves per treatment: 1) intact bulls (BULL), 2) castration by banding (BAN), or 3) castration by banding with …
Effect Of Meloxicam On Gain And Behavior Of Calves Castrated By Banding Pre-Weaning,
2013
Berry College
Effect Of Meloxicam On Gain And Behavior Of Calves Castrated By Banding Pre-Weaning, Joseph A. Daniel, Peter D. Krawczel, Brian K. Whitlock
Brian K Whitlock, PhD, DVM, DACT
The objective was to determine if oral meloxicam (M; a non-steroid anti-inflammatory) administered at castration of pre-weaning calves affected ADG or behavior. Prior to castration (14 d), Angus bulls were assigned to bull (BULL; n = 7; age 106 ± 6 d; BW = 174.2 ±7.7 kg; scrotal circumference 17.7 ± 0.4 cm), castrated (BAN; n = 12; age = 105 ± 5 d; BW = 144.5 ± 7.6 kg; scrotal circumference = 16 ± 0.4 cm) or castrated with meloxicam (BAN+M; n = 13; age = 121 ± 6 d; BW = 145.8 ± 6 kg; scrotal circumference = …
Meloxicam Mediates Short-Term Behavioral Changes Of Castrated Calves,
2013
University of Tennessee - Knoxville
Meloxicam Mediates Short-Term Behavioral Changes Of Castrated Calves, Peter D. Krawczel, Jeff A. Carroll, Nicole C. Burdick Sanchez, Jeff W. Dailey, Joseph A. Daniel, Johann F. Coetzee, Brian K. Whitlock
Brian K Whitlock, PhD, DVM, DACT
Castration may detrimentally affect the health and performance of weaned calves and painful procedures are increasingly a public concern. Therefore, practical pain mitigation is critical. The objective was to determine the effects of castration (by banding) with or without administration of NSAID, meloxicam, on the behavior of weaned beef calves. Forty-eight (56 d post-weaning) beef calves [10.0 ± 0.2 (mean ± SE) mo old; 304 ± 6 kg BW] were blocked by multiple factors then randomly assigned to 3 treatments (n = 16 calves per treatment: 1) intact bulls (BULL), 2) castration by banding (BAN), or 3) castration by banding …
Evaluating The Formation Of Bacterial Chondronecrosis With Osteomyelitis In Broilers Raised On Portable Wire Floor Models,
2013
University of Arkansas, Fayetteville
Evaluating The Formation Of Bacterial Chondronecrosis With Osteomyelitis In Broilers Raised On Portable Wire Floor Models, Alex David Gilley
Graduate Theses and Dissertations
Rearing birds on wire flooring has proven to be an effective method for consistently triggering significant levels of lameness in broilers, and while the wire flooring model has been proven effective, more mobile wire floor models have been developed to compliment the facility management conditions within commercial genetic selection programs. The mobile wire floor models have been titled "speed bumps", and through previous evaluation have resulted in significantly higher levels of lameness in broilers compared to litter floor shavings. The current experiment contains two studies (studies 1 and 2). Both studies involved comparing leg weakness between broiler strains; while in …
Genome-Wide Analysis Reveals Selection For Important Traits In Domestic Horse Breeds,
2013
University of Nebraska-Lincoln
Genome-Wide Analysis Reveals Selection For Important Traits In Domestic Horse Breeds, Jessica Lynn Petersen, James R. Mickelson, Aaron K. Rendahl, Stephanie K. Valberg, Lisa S. Andersson, Ernie Bailey, Danika L. Bannasch, Matthew M. Binns, Alexandre S. Borges, Pieter Brama, Artur Da Câmara Machado, Stefano Capomaccio, Katia Cappelli, E. Gus Cothran, Ottmar Distl, Laura Fox-Clipsham, Kathryn T. Graves, Gérard Guérin, Bianca Haase, Telhia Hasegawa, Karin Hemmann, Emmeline W. Hill, Tosso Leeb, Gabriella Lindgren, Hannes Lohi, Maria Susana Lopes, Beatrice A. Mcgivney, Sofia Mikko, Nicholas Orr, M. Cecilia T. Penedo, Richard J. Piercy, Marja Raekallio, Stefan Rieder, Knut H. Røed, June Swinburne, Teruaki Tozaki, Mark Vaudin, Claire M. Wade, Molly E. Mccue
Faculty Papers and Publications in Animal Science
Intense selective pressures applied over short evolutionary time have resulted in homogeneity within, but substantial variation among, horse breeds. Utilizing this population structure, 744 individuals from 33 breeds, and a 54,000 SNP genotyping array, breed-specific targets of selection were identified using an FST-based statistic calculated in 500-kb windows across the genome. A 5.5-Mb region of ECA18, in which the myostatin (MSTN) gene was centered, contained the highest signature of selection in both the Paint and Quarter Horse. Gene sequencing and histological analysis of gluteal muscle biopsies showed a promoter variant and intronic SNP of MSTN were …
Immune Responses In Lactating Holstein Cows Supplemented With Cu, Mn, And Zn As Sulfates Or Methionine Hydroxy Analogue Chelates,
2012
University of Delaware
Immune Responses In Lactating Holstein Cows Supplemented With Cu, Mn, And Zn As Sulfates Or Methionine Hydroxy Analogue Chelates, L. M. Nemec, J. D. Richards, C. A. Atwell, D. E. Diaz, G. I. Zanton, T. F. Gressley
Physiology Collection
The aim of this study was to compare effects of inorganic sulfate versus chelated forms of supplemental Cu, Mn, and Zn on milk production, plasma and milk mineral concentrations, neutrophil activity, and antibody titer response to a model vaccination. Holstein cows (n = 25) were assigned in 2 cohorts based on calving date to a 12-wk randomized complete block design study. The first cohort consisted of 17 cows that had greater days in milk (DIM; mean of 77 DIM at the start of the trial) than the second cohort of 8 cows (32 DIM at the start of the trial). …
Hovering Energetics And Thermal Balance In Anna's Hummingbirds (Calypte Anna),
2012
University of California - Berkeley
Hovering Energetics And Thermal Balance In Anna's Hummingbirds (Calypte Anna), Dennis Evangelista, Maria J. Fernandez, Madalyn S. Berns, Aaron M. Hoover, Robert Dudley
Aaron M. Hoover
We studied the energetics of hover-feeding Anna's hummingbirds, using three different simultaneous techniques: heat loss as estimated via thermal imaging, metabolic rate as measured at a feeder mask using flow-through respirometry, and aerodynamic power estimated from wingbeat kinematic data. These three methods yielded comparable estimates of power output at ambient air temperatures ranging from 18 degrees to 26 degrees C, whereas heat imbalance at higher air temperatures (up to 34 degrees C) suggested loss by mechanisms other than convection and radiation from the body, such as evaporative cooling and enthalpy rise associated with exhaled air and excreted water and convective …
Does Immunocontraception In Feral Horses (Equus Caballus) Extend Reproductive Cycling Beyond The Normal Breeding Season?,
2012
Virginia Polytechnic Institute and State University
Does Immunocontraception In Feral Horses (Equus Caballus) Extend Reproductive Cycling Beyond The Normal Breeding Season?, Cassandra M.V. Nuñez, James S. Adelman, Daniel I. Rubenstein
Cassandra M.V. Nuñez
Although the physiological effects of immunocontraceptive treatment with porcine zona pellucida (PZP) have been well studied, little is known about PZP’s effects on the scheduling of reproductive cycling. Recent behavioral research has suggested that recipients of PZP extend the receptive breeding period into what is normally the non-breeding season. To determine if this is the case, we compiled foaling data from wild horses (Equus caballus) living on Shackleford Banks, North Carolina for 4 years pre- and 8 years post-contraception management with PZP (pre-contraception, n = 65 births from 45 mares; post-contraception, n = 97 births from 46 mares). Gestation lasts …
Characterisation Of Chemosensory Trigeminal Receptors In The Rainbow Trout, Oncorhynchus Mykiss: Responses To Chemical Irritants And Carbon Dioxide,
2012
University of Liverpool
Characterisation Of Chemosensory Trigeminal Receptors In The Rainbow Trout, Oncorhynchus Mykiss: Responses To Chemical Irritants And Carbon Dioxide, Jessica J. Mettam, Catherine R. Mccrohan, Lynne U. Sneddon
Veterinary Science and Medicine Collection
Trigeminally innervated, mechanically sensitive chemoreceptors (M) were previously identified in rainbow trout, Oncorhynchus mykiss, but it is not known whether these receptors are responsive only to noxious, chemical irritants or have a general chemosensory function. This study aimed to characterise the stimulus–response properties of these receptors in comparison with polymodal nociceptors (P). Both P and M gave similar response profiles to acetic acid concentrations. The electrophysiological properties were similar between the two different afferent types. To determine whether the receptors have a nociceptive function, a range of chemical stimulants was applied to these receptors, including non-noxious stimuli such as ammonium …
Mammary Cell Activity And Turnover In Dairy Cows Treated With The Prolactin-Release Inhibitor Quinagolide And Milked Once Daily, M. Boutinaud, V. Lollivier, L. Finot, R. M. Bruckmaier, P. Lacasse
Physiology Collection
To assess the regulation of mammary cell activity, survival, and proliferation by prolactin (PRL), 5 Holstein cows in early lactation received daily i.m. injections of 1 mg of quinagolide, a suppressor of PRL release, for 9 wk, whereas 4 control cows received the vehicle (water) only. During the last week of treatment, one udder half was milked once a day (1×) and the other twice a day (2×). Mammary biopsies were harvested 1 wk before and 4 and 8 wk after the start of quinagolide treatment. The quinagolide injections reduced milk yield and resulted in lower levels of κ-casein and …
A Non-Invasive Assay For Monitoring Stress Responses: A Comparison Between Wild And Captive-Reared Rainbowfish (Melanoteania Duboulayi),
2011
Macquarie University
A Non-Invasive Assay For Monitoring Stress Responses: A Comparison Between Wild And Captive-Reared Rainbowfish (Melanoteania Duboulayi), Amina Zuberi, Sinan Ali, Culum Brown
Aquaculture Collection
The stress response of wild and captive reared rainbowfish (Melanoteania duboulayi) following chasing by a simulated predator was examined. Cortisol release rate was monitored using a flow through system by measuring water borne hormone levels. Tests using known cortisol concentrations revealed that the technique yielded 95% of the cortisol present in the water. Cortisol release rates increased several fold in both populations after being chased but peaked at different time periods. Wild fish showed a typical stress response with release rate rising to (2.29±0.22 ng g−1 h−1) 2 h after exposure followed by rapid recovery. The captive-reared …
Heifer Estrous Cycle Stage Impacts Luteinizing And Growth Hormone Response To Kisspeptin,
2011
University of Tennessee - Knoxville
Heifer Estrous Cycle Stage Impacts Luteinizing And Growth Hormone Response To Kisspeptin, Brian K. Whitlock, Joseph A. Daniel, Robyn R. Wilborn, Barbara P. Steele, James L. Sartin
Brian K Whitlock, PhD, DVM, DACT
The reproductive neuropeptide, kisspeptin (KP), may have a role in regulation of growth hormone (GH) secretion. Expression of KP and its receptor (Kiss1r) are developmentally and hormonally regulated. This study was conducted to determine the effects of KP on plasma concentrations of luteinizing hormone (LH) and GH in pubertal heifers during different functional reproductive stages. Heifers received a single injection of KP (100 pmole / kg) or saline intravenously during proestrus, estrus, and diestrus, and serial blood samples were collected for 3 hours to determine the response of both LH and GH after treatment. Mean concentrations of LH were increased …
Heifer Estrous Cycle Stage Impacts Luteinizing And Growth Hormone Response To Kisspeptin,
2011
University of Tennessee - Knoxville
Heifer Estrous Cycle Stage Impacts Luteinizing And Growth Hormone Response To Kisspeptin, Brian K. Whitlock, Joseph A. Daniel, Robyn R. Wilborn, Barbara P. Steele, James L. Sartin
Animal Science Publications and Other Works
The reproductive neuropeptide, kisspeptin (KP), may have a role in regulation of growth hormone (GH) secretion. Expression of KP and its receptor (Kiss1r) are developmentally and hormonally regulated. This study was conducted to determine the effects of KP on plasma concentrations of luteinizing hormone (LH) and GH in pubertal heifers during different functional reproductive stages. Heifers received a single injection of KP (100 pmole / kg) or saline intravenously during proestrus, estrus, and diestrus, and serial blood samples were collected for 3 hours to determine the response of both LH and GH after treatment. Mean concentrations of LH were increased …
Effect Of Kisspeptin On Regulation Of Growth Hormone,
2011
University of Tennessee - Knoxville
Effect Of Kisspeptin On Regulation Of Growth Hormone, Brian K. Whitlock, Joseph A. Daniel, Robyn R. Wilborn, Herris S. Maxwell, Barbara P. Steele, James L. Sartin
Brian K Whitlock, PhD, DVM, DACT
Kisspeptin ( KP ), a neuroendocrine regulator of reproduction, is hypothesized to be an integrator of metabolism and hormones critical to the regulation of reproduction. Lactation is associated with enhanced growth hormone (GH) responsiveness and reduced fertility. Our study was designed to determine the effects of lactation on KP-stimulated GH and luteinizing hormone (LH) secretion. Five non-lactating and five lactating dairy cows were used in the study. Experiments were conducted with lactating cows at weeks 1, 5 and 11 after parturition. The experimental treatments ( saline and KP [100 and 400 pmol / kg body weight]) were given intravenously and …
Effect Of Kisspeptin On Regulation Of Growth Hormone,
2011
University of Tennessee - Knoxville
Effect Of Kisspeptin On Regulation Of Growth Hormone, Brian K. Whitlock, Joseph A. Daniel, Robyn R. Wilborn, Herris S. Maxwell, Barbara P. Steele, James L. Sartin
Animal Science Publications and Other Works
Kisspeptin ( KP ), a neuroendocrine regulator of reproduction, is hypothesized to be an integrator of metabolism and hormones critical to the regulation of reproduction. Lactation is associated with enhanced growth hormone (GH) responsiveness and reduced fertility. Our study was designed to determine the effects of lactation on KP-stimulated GH and luteinizing hormone (LH) secretion. Five non-lactating and five lactating dairy cows were used in the study. Experiments were conducted with lactating cows at weeks 1, 5 and 11 after parturition. The experimental treatments ( saline and KP [100 and 400 pmol / kg body weight]) were given intravenously and …
The Effect Of Kisspeptin Receptor Agonist (Ftm080) On Luteinizing Hormone In Sheep,
2011
University of Tennessee - Knoxville
The Effect Of Kisspeptin Receptor Agonist (Ftm080) On Luteinizing Hormone In Sheep, Brian K. Whitlock, Joseph A. Daniel, Maria Hes, Barbara P. Steele, James L. Sartin, S. Oishi, N. Fujii
Brian K Whitlock, PhD, DVM, DACT
Kisspeptin receptor (Kiss1r) agonists with increased half-life and similar efficacy to kisspeptin (KP) in vitro potentially may provide beneficial applications in breeding management of many species. However, many of these agonists have not been tested in vivo. This study was designed to test the effect of a Kiss1r agonist (FTM080) on luteinizing hormone (LH) in vivo. Sheep were treated with FTM080 (500 pmol/kg BW) or sterile water in a 2-ml bolus via the jugular cannula. Serial blood samples were collected every 15-min before (1 hr) and after (1 hr) treatment. Intravenous (IV) injection of FTM080 increased (P < 0.05) LH plasma concentrations through the 45-min sample following treatment. Moreover, the area under the curve of LH in the period from 0 to 60-min following FTM080 treatment was also increased (P < 0.05). These data provide evidence to suggest that FTM080 stimulates the gonadotropic axis of ruminants in vivo.
