Producing A Subunit Vaccine For Porcine Epidemic Diarrhea Virus, 2016 The University of Western Ontario
Producing A Subunit Vaccine For Porcine Epidemic Diarrhea Virus, Zayn Khamis
Electronic Thesis and Dissertation Repository
Porcine epidemic diarrhea virus (PEDv) causes disease and mortality to piglets worldwide. Most vaccines used to combat the disease have been ineffective live attenuated virus vaccines. The goal of this project was to produce a plant-made subunit vaccine based off the membrane protein of the virus. This is the first time this protein has been produced in plants. An elastin-like polypeptide fusion membrane protein accumulated up to 0.8 mg/g of fresh leaf weight when transiently expressed in Nicotiana benthamiana. Virus-like particles were also produced for the first time for PEDv, and were able to form with just the membrane …
The Lowdown Of Out-Of-Competition Testing: What The Rmtc Isn't Telling Us About Its Proposed Regulations, 2016 University of Kentucky
The Lowdown Of Out-Of-Competition Testing: What The Rmtc Isn't Telling Us About Its Proposed Regulations, Clara Fenger, Tanya Boulmetis, Kim Brewer, Thomas Tobin
Maxwell H. Gluck Equine Research Center Faculty Publications
No abstract provided.
Allelic Variation In Cxcl16 Determines Cd3+ T Lymphocyte Susceptibility To Equine Arteritis Virus Infection And Establishment Of Long-Term Carrier State In The Stallion, 2016 University of Kentucky
Allelic Variation In Cxcl16 Determines Cd3+ T Lymphocyte Susceptibility To Equine Arteritis Virus Infection And Establishment Of Long-Term Carrier State In The Stallion, Sanjay Sarkar, Ernest Bailey, Yun Young Go, R. Frank Cook, Ted Kalbfleisch, John E. Eberth, R. Lakshman Chelvarajan, Kathleen M. Shuck, Sergey Artiushin, Peter J. Timoney, Udeni B. R. Balasuriya
Maxwell H. Gluck Equine Research Center Faculty Publications
Equine arteritis virus (EAV) is the causative agent of equine viral arteritis (EVA), a respiratory, systemic, and reproductive disease of horses and other equid species. Following natural infection, 10–70% of the infected stallions can become persistently infected and continue to shed EAV in their semen for periods ranging from several months to life. Recently, we reported that some stallions possess a subpopulation(s) of CD3+ T lymphocytes that are susceptible to in vitro EAV infection and that this phenotypic trait is associated with long-term carrier status following exposure to the virus. In contrast, stallions not possessing the CD3+ T …
Assessing Readiness For Clinical Practice: Students’ Perspectives Of Their Veterinary Curriculum, 2016 University of Florida College of Veterinary Medicine
Assessing Readiness For Clinical Practice: Students’ Perspectives Of Their Veterinary Curriculum, L. Chris Sanchez, Alison Kwiatkowski, Jeff Abbott, Dana N. Zimmel, Linda S. Behar-Horenstein
The Qualitative Report
Studies describing the effectiveness of a veterinary curriculum from the student perspective are currently sparse. The overall purpose of this investigation was to describe students’ perceived preparedness for clinical practice. Three focus group meetings with fourth year veterinary students were conducted. Data were open-coded and categorized to identify themes. Four main themes emerged: Challenging communications, Un/appreciating curricular experiences, Documenting demands impede case involvement, and Hungering for timely, effective feedback. Overall students felt comfortable talking to clients about medicine but less comfortable discussing euthanasia or money; they appreciated the split clinical curriculum but questioned the value of the 1st/2nd year courses; …
2016 Study Of Current Conditions Of Kentucky County Animal Shelters And Degree Of Compliance With Kentucky Animal Shelter Laws, 2016 University of Kentucky
2016 Study Of Current Conditions Of Kentucky County Animal Shelters And Degree Of Compliance With Kentucky Animal Shelter Laws, Cynthia L. Gaskill, Rachel Cullman-Clark, Liane Lachiewicz, Matt Lamarre, Brad Rohleder, Kristin Sadler, Rachel Sparling, Craig N. Carter
Veterinary Science Reports
Kentucky’s county animal shelter conditions have not been studied for over 20 years. Major goals of this study were to assess current conditions in Kentucky’s county shelters and determine the degree of compliance with Kentucky shelter laws. Additional information was gathered to determine the major problems and needs identified by shelter personnel and researchers. Data was used to determine if additional state funds or refinements and additions to current laws are warranted to ensure humane care of animals in Kentucky’s county shelters. Researchers consisted of a group of 6 veterinary students who traveled to all Kentucky county animal shelters. Ninety …
Animal Health Matters, 2016 South Dakota State University
Animal Health Matters, Jane Hennings, Larry Holler, Russ Daly, Linda Fawcett
Animal Health MATTERS Newsletter
[Page] 1 SDSU and SHIC Study on Senecavirus Offers Keen Insights
[Page] 2 Director's Message: Innovation in the Laboratory and Being Proactive in the Protection of Animal Health
[Page] 2 Bovine Abortion-Sample Submission Guidelines
[Page] 3 South Dakota One Health Meeting Covers Bacterial Zoonotic Disease
[Page] 3 Small Ruminant, Poultry Serology Offerings Expanded at the ADRDL
[Page] 4 Molecular Diagnostics Section Evolves, Adds New Tests
[Page] 5 Recent PCR tests Added by ADRDL
[Page] 6 New Employees
[Page] 7 ADRDL Continues Their Critical Role Supporting Animal Exports
Evaluation Of Several Pre-Clinical Tools For Identifying Characteristics Associated With Limb Bone Fracture In Thoroughbred Racehorses, 2016 Purdue University
Evaluation Of Several Pre-Clinical Tools For Identifying Characteristics Associated With Limb Bone Fracture In Thoroughbred Racehorses, Anthony Nicholas Corsten
Open Access Theses
Catastrophic skeletal fractures in racehorses are devastating not only to the animals, owners and trainers, but also to the perception of the sport in the public eye. The majority of these fatal accidents are unlikely to be due to chance, but are rather an end result failure from stress fractures. Stress fractures are overuse injuries resulting from an accumulation of bone tissue damage over time. Because stress fractures are pathological, it is possible that overt fractures can be predicted and prevented. In this study, third metacarpals (MC3) from 33 thoroughbred racehorse comprised of 8 non-fractured controls and 25 horses that …
The Impact Of Tickling Rats On Human-Animal Interactions And Rat Welfare, 2016 Purdue University
The Impact Of Tickling Rats On Human-Animal Interactions And Rat Welfare, Megan Renee Lafollette
Open Access Theses
Rats initially fear humans which can lead to negative affect, poor welfare, and difficult handling. Also, modeling and measuring positive affect states in rats can pose an additional challenge. Heterospecific play, or “tickling,” is a handling habituation technique that mimics rat rough-and-tumble play that is being used to study positive affect. It can also be used to reduce fear of human, improve welfare, and elicit a positive affect state. However, current studies implementing the technique in laboratory rats use a wide variety of protocols to achieve differential results. Unlike in laboratory environment, pet store rats experience high levels of novelty …
Effect Of Ivprd, Estradiol And Gnrh For Resynchronization On Fertility Of Non-Pregnant Holstein Dairy Cows, 2016 Chulalongkorn University
Effect Of Ivprd, Estradiol And Gnrh For Resynchronization On Fertility Of Non-Pregnant Holstein Dairy Cows, Reza Narenji Sani, Ali Mahdavi, Jalal Gharavi Ghare Mashk
The Thai Journal of Veterinary Medicine
The aim of this study was to evaluate the effect of intra vaginal progesterone releasing device (IVPRD), estradiol and GnRH for resynchronization on fertility of non-pregnant Holstein dairy cows. Non-pregnant cows (n = 117) were divided into three groups. The first group (n = 38) was treated with Ovsynch [GnRH, day 0; PGF2α, day 7; GnRH, day 9; timed artificial insemination (TAI), day 10]. The second group (n = 39) was treated with cue-mate-estradiol benzoate (EB) (cue-mate intra vaginal insertion and first EB, day 0; PGF2α, day 7; cue-mate withdrawn and second EB, day 8; TAI, day 10). The third …
Field Study Of Seroconversion Of Three Different Commercial Vaccines Of Chicken Infectious Anemia Virus In Thailand, 2016 Chulalongkorn University
Field Study Of Seroconversion Of Three Different Commercial Vaccines Of Chicken Infectious Anemia Virus In Thailand, Niwat Chansiripornchai
The Thai Journal of Veterinary Medicine
The aim of this study was to evaluate the effects of different chicken infectious anemia virus (CIAV) vaccine strains in breeders and progeny under field conditions. For this experiment, one commercial poultry company was selected, and independent management for each house was applied to avoid vaccine cross contamination. No natural infection was found before vaccination. BioChek ELISA kits were used, and a minimum of 20 birds per flock were sampled in each sampling time. Three commercial CIAV vaccine strains that are available in Thailand were selected for the study: 26P4 was vaccinated subcutaneously, Cux-1 was vaccinated orally, and Del Ros …
Advanced Biotechnologies For Wildlife Fertility Preservation, 2016 Chulalongkorn University
Advanced Biotechnologies For Wildlife Fertility Preservation, Pierre Comizzoli
The Thai Journal of Veterinary Medicine
Reproductive biotechnologies are critical tools for saving and maintaining endangered species. Some successes have been reported with the use and integration of artificial insemination (with fresh or frozen-thawed semen) in conservation programs. However, not a single species is currently managed through oocyte freezing or embryo-based technologies. This is primarily due to the lack of knowledge of species biology, as well as inadequate facilities, space, expertise, and funding needed for their successful application. More fundamental studies of animal reproductive biology as well as more fertility preservation options are needed with all parties involved (reproductive technologists, zoo biologists and conservationists) adopting parallel …
Effects Of Β-Glucan And Mannan-Oligosaccharide Supplementation On Growth Performance, Fecal Bacterial Population, And Immune Responses Of Weaned Pigs, 2016 Chulalongkorn University
Effects Of Β-Glucan And Mannan-Oligosaccharide Supplementation On Growth Performance, Fecal Bacterial Population, And Immune Responses Of Weaned Pigs, Phan Thi Tuoi, Pornchalit Assavacheep, Kris Angkanaporn, Anongnart Assavacheep
The Thai Journal of Veterinary Medicine
The effects of β-glucan and mannan-oligosaccharide (BG + MOS) supplementation in feed on growth performance, bacterial population in feces and immune responses of weaned pigs were investigated. Two hundred and eighty-eight weaning piglets were randomly allocated into 3 groups with 8 replicates per group and 12 pigs per replicate. Group 1, as control, was fed a commercial basal nursery diet. Groups 2 and 3 were fed the same diet supplemented with BG + MOS (Biolex® MB-40) 1 and 2 kg/ton of feed, respectively. Weight gain (WG), average daily gain (ADG), average daily feed intake (ADFI) and feed conversion ratio (FCR) …
Modulatory Effects Of Pg102 (Actinidia Arguta) On Inflammatory Cytokine Mrna Expression In Canine Atopic Dermatitis, 2016 Chulalongkorn University
Modulatory Effects Of Pg102 (Actinidia Arguta) On Inflammatory Cytokine Mrna Expression In Canine Atopic Dermatitis, Yong-Uk Lee, Seol-Hee Park, Eui-Hwa Nam, Tae-Ho Chung, Cheol-Yong Hwang
The Thai Journal of Veterinary Medicine
Canine atopic dermatitis (AD) is a common, chronic relapsing inflammatory skin disease. An aqueous extract, PG102, derived from Actinidia arguta fruit, has been investigated as an anti-inflammatory agent in humans and atopy-like animal models, but its underlying mechanism for canine AD treatment has not been fully understood. This study evaluated the in vitro anti-inflammatory effect of PG102 with respect to the representative allergy-related cytokine panel from dust mite allergen extract (DME)-stimulated peripheral blood mononuclear cells (PBMCs) of canine AD patients using quantitative real-time PCR. PBMCs were isolated from eight dogs with definite diagnoses of AD and stimulated with DME for …
Renoprotective Effects Of Megadose Vitamin C On Cisplatin-Induced Kidney Injury, 2016 Chulalongkorn University
Renoprotective Effects Of Megadose Vitamin C On Cisplatin-Induced Kidney Injury, Supa Sithanukul, Chollada Buranakarl, Chutamas Benjanirut, Anudep Rungsipipat
The Thai Journal of Veterinary Medicine
Cisplatin (CDDP)-induced nephrotoxicity is mediated via oxidative stress and may be alleviated using antioxidant activity. Although megadose of vitamin C may cause oxalate nephropathy and apoptosis, the antioxidant effect of vitamin C may be beneficial when given along with CDDP. The objective of this study was to investigate the renoprotective effects of megadose vitamin C on rats receiving CDDP. Rats were divided into 4 groups: group 1, control rats (CONT); group 2, vitamin C-treated rats (VIT C); group 3, CDDP-treated rats (CDDP) and group 4, CDDP + vitamin C-treated rats (CDDP + VIT C). Vitamin C (1000 mg/kg) was given …
Dietary Supplementation Of Condensed Tannin Decreased Lipid Peroxidation In Muscle Of Nile Tilapias (Oreochromis Niloticus), 2016 Chulalongkorn University
Dietary Supplementation Of Condensed Tannin Decreased Lipid Peroxidation In Muscle Of Nile Tilapias (Oreochromis Niloticus), Natthana Thitichayaphong, Theera Rukkwamsuk
The Thai Journal of Veterinary Medicine
The effect of condensed tannin supplementation in diet on lipid peroxidation in the muscle was evaluated in Nile tilapias (Oreochromis niloticus). In total, 250 fish, aged 21 days old, were randomly allocated into 10 experimental tanks, 25 fish per tank. Five tanks were set as the control group and 5 tanks as the supplemented group. The control fish were fed a commercial diet, while the supplemented fish were fed the same control diet plus 4 g/kg of condensed tannin. The diets were offered at a rate of 8% of estimated fish body weight twice daily. The experimental period lasted for …
Chikungunya Virus Infection In Balb/C And Icr Mouse Models, 2016 Chulalongkorn University
Chikungunya Virus Infection In Balb/C And Icr Mouse Models, Sonthaya Tiawsirisup, Ranida Tuanudom, Nichapat Yurayart
The Thai Journal of Veterinary Medicine
Chikungunya virus (CHIKV) infection is an emerging or re-emerging infectious disease found in several countries. The ecology of this virus involves humans, reservoir animals, and mosquito vectors. Two experiments were conducted in this study. Blood samples were collected and tested for CHIKV infection by using immunocytochemistry (ICC) staining and reverse transcription polymerase chain reaction (RT-PCR). For the first experiment, BALB/c mice were inoculated with 106.3 CID50/mouse. Mean CHIKV titers in the BALB/c mice were 103.3, 101.5, 102.3, and 102.3 CID50/ml of serum on day 1 to day 4 post inoculation (PI), respectively. For the second experiment, there were two groups …
Ophthalmology Snapshot, 2016 Chulalongkorn University
Ophthalmology Snapshot, Nalinee Tuntivanich
The Thai Journal of Veterinary Medicine
No abstract provided.
Regulation Of Alpha-Herpesvirus Reactivation From Latency By Stress, 2016 University of Nebraska - Lincoln
Regulation Of Alpha-Herpesvirus Reactivation From Latency By Stress, Insun Kook
School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research
Bovine herpes virus 1 (BHV-1) and Herpes simplex virus 1 (HSV-1) are crucial etiological viral agent of clinical diseases. HSV-1 and BHV-1 establish latent infection in sensory neurons. Periodically, reactivation from latency occurs resulting in virus excretion and transmission. Stress increases corticosteroid levels and the incidence of HSV-1 and BHV-1 reactivation from latency. The synthetic corticosteroid, dexamethasone (DEX) mimics stress and induces BHV-1 and HSV-1 reactivation. However, molecular mechanisms by which corticosteroid mediates viral reactivation are not well understood. My dissertation has focused on elucidating events that induce BHV- 1 or HSV-1 reactivation during the early stages of stress-induced escape …
Follicular Dynamics In Insulin Resistant Mares, 2016 University of Tennessee, Knoxville
Follicular Dynamics In Insulin Resistant Mares, Julio Cesar Prado
Masters Theses
Obesity and insulin resistance have been linked to prolonged interovulatory period, aberrations in the estrous cycle, and continuous reproductive activity during the non-breeding season. EMS has been determined to influence the intrafollicular environment of mare ovaries. In humans, insulin resistance has been linked to polycystic ovaries as part of Polycystic Ovarian Syndrome (PCOS). A study was conducted to determine 1) the impact of insulin resistance on follicle growth and size at ovulation, and 2) whether predicted ovulatory follicles respond to hCG administration in Insulin-resistant (IR) mares. Mares were selected for the study based on insulin sensitivity and separated into an …
Prevalence Of Bartonella Infection In Well-Cared Cats In Bangkok Metropolitan, 2016 Chulalongkorn University
Prevalence Of Bartonella Infection In Well-Cared Cats In Bangkok Metropolitan, Pakkawan Satranarakun, Soichi Maruyama, Hidori Kabeya, Shigo Sato, Sathaporn Jitapalapong, Saowalak Jitchum, Tawisa Jiyipong, Channarong Rodkhum, Rosama Pusoonthornthum
The Thai Journal of Veterinary Medicine
The study was conducted to determine the prevalence of bartonellosis in two hundred well-cared client-owned cats that have close relationship with their owners in Bangkok metropolitan area from November 2010 to November 2011. These cats had no clinical signs at the time of presentation and no visual flea infestation. Blood collection was performed and cultured in 5% rabbit blood agar. Species of Bartonella spp. was identified with Polymerase Chain Reaction and gene sequencing. Results demonstrated that eleven out of the two hundred cats were positive for bartonellosis. Moreover, the prevalence was 5.5% (11/200), with Bartonella henselae 91% (10/11) and Bartonella …