P27kip1 And P130 Cooperate To Regulate Hematopoietic Cell Proliferation In Vivo, 2016 Hammersmith Hospital
P27kip1 And P130 Cooperate To Regulate Hematopoietic Cell Proliferation In Vivo, Inês Soeiro, Azim Mohamedali, Hanna M. Romanska, Nicholas C. Lea, Emma S. Child, Janet Glassford, Stephen J. Orr, Claudia Roberts, Kikkeri N. Naresh, El-Nasir Lalani, David J. Mann, Roger J. Watson, N. Shaun B. Thomas, Eric W. -F. Lam
El Nasir Lalani
To investigate the potential functional cooperation between p27Kip1 and p130 in vivo, we generated mice deficient for both p27Kip1 and p130. In p27Kip1−/−;p130−/− mice, the cellularity of the spleens but not the thymi is significantly increased compared with that of their p27Kip1−/− counterparts, affecting the lymphoid, erythroid, and myeloid compartments. In vivo cell proliferation is significantly augmented in the B and T cells, monocytes, macrophages, and erythroid progenitors in the spleens of p27Kip1−/−; p130−/− animals. Immunoprecipitation and immunodepletion studies indicate that p130 can compensate for the absence of p27Kip1 in binding to and ...
Comparing Direct Factor Xa Inhibitors And Warfarin In The Prevention Of Stroke In Patients With Atrial Fibrillation, 2016 James Madison University
Comparing Direct Factor Xa Inhibitors And Warfarin In The Prevention Of Stroke In Patients With Atrial Fibrillation, Alessandra Lof, Stephanie Pillai
Physician Assistant Capstones
Objective: To evaluate the overall efficacy, advantages, and disadvantages of treatment with direct factor Xa inhibitors as compared to warfarin in the prevention of stroke in patients with atrial fibrillation. Methods: A quantitative meta-analysis was performed on three separate studies, each of which evaluated the efficacy and safety outcomes of a direct factor Xa inhibitor versus warfarin in preventing stroke in patients with atrial fibrillation. The direct factor Xa inhibitors that were evaluated included apixaban, edoxaban, and rivaroxaban. Results: The direct factor Xa inhibitors were found to be as effective, and in some cases more effective, than warfarin in preventing ...
Discovery And Development Of Human Monoclonal Antibodies To Block Rhd Alloimmunization During Pregnancy, 2016 University of Massachusetts Medical School
Discovery And Development Of Human Monoclonal Antibodies To Block Rhd Alloimmunization During Pregnancy, Tushar Gupta, Melissa Gawron, Colby A. Souders, Michael A. Brehm, Dale Greiner, Leonard D. Shultz, Smita Jaiswal, Sean Mccauley, Ann Dauphin, Jeremy Luban, Lisa Cavacini
UMass Center for Clinical and Translational Science Research Retreat
Exposure of an Rh negative mother to red blood cells (RBCs) of an Rh positive fetus results in alloimmunization and development of anti-RhD antibodies. The anti-RhD antibodies cause hemolytic disease of the new born babies during subsequent pregnancies. Current prophylactic treatment involves polyclonal anti-RhD IgG purified from plasma of humans and is administered in approximately 20% of pregnancies. While the current prophylaxis is effective, it involves the use of human plasma and non-RhD specific antibodies, thus posing a risk of transmitting infections and undesired antibody reactions. Moreover, there is a serious scarcity of plasma donors to meet the requirement of ...
The Mysteries Of Your Blood, 2016 Butler University
The Mysteries Of Your Blood, Alexandra J. Mauer
Did you know that your blood type can be useful for determining disease susceptibility and even creating a diet that works best for your personal metabolism? The Mysteries of your Blood is an article that delves into the science behind the ABO blood types and explains why your body can handle certain foods, stress, and illness differently than someone with a different blood type. It proposes a new, perplexing way to determine how to avoid certain health problems while at the same time offering suggestions of what to eat or, rather, what to avoid eating.
To What Extent Did Blood Transfusion Systems And Technologies Modernize During World War Ii?, 2016 Sunset High School
To What Extent Did Blood Transfusion Systems And Technologies Modernize During World War Ii?, Hannah J. Leblanc
Young Historians Conference
This investigation will explore changes in blood transfusion during World War II (1939-1945). Medical technology and collection systems for whole blood and blood plasma in particular will be examined. The focus of this investigation will be the United States, but Great Britain will also be mentioned due to its close blood transfusion-related interactions with the United States during this period. Additionally, blood transfusion prior to World War II and in modern times will also be considered in this investigation to provide context and to allow assessment of modernization during the war. However, artificial blood substitutes will not be considered, nor ...
Primary Malignant Pleural Effusion With A Profound Type B Lactic Acidosis, 2016 Lehigh Valley Health Network
Primary Malignant Pleural Effusion With A Profound Type B Lactic Acidosis, Rachel Kinney Do, Christine Saraceni Do, Eliot L. Friedman Md
Department of Medicine
No abstract provided.
A Case Report Of Ruxolitinib Induced Hypocalcemia: A Stochastic Or Deterministic Effect?, 2016 Lehigh Valley Health Network
A Case Report Of Ruxolitinib Induced Hypocalcemia: A Stochastic Or Deterministic Effect?, Arsha Sreedhar Md, Ranjit R. Nair Md, Gretchen A. Perilli Md
Department of Medicine
No abstract provided.
A Case-Control Genome-Wide Association Study Identifies Genetic Modifiers Of Fetal Hemoglobin In Sickle Cell Disease., 2016 University of Texas at Dallas
A Case-Control Genome-Wide Association Study Identifies Genetic Modifiers Of Fetal Hemoglobin In Sickle Cell Disease., Li Liu, Alexander Pertsemlidis, Liang-Hao Ding, Michael D Story, Martin H Steinberg, Paola Sebastiani, Carolyn Hoppe, Samir K. Ballas, Betty S Pace
Cardeza Foundation for Hematologic Research
Sickle cell disease (SCD) is a group of inherited blood disorders that have in common a mutation in the sixth codon of the β-globin (HBB) gene on chromosome 11. However, people with the same genetic mutation display a wide range of clinical phenotypes. Fetal hemoglobin (HbF) expression is an important genetic modifier of SCD complications leading to milder symptoms and improved long-term survival. Therefore, we performed a genome-wide association study (GWAS) using a case-control experimental design in 244 African Americans with SCD to discover genetic factors associated with HbF expression. The case group consisted of subjects with HbF≥8.6 ...
Trends In Survival Of Chronic Lymphocytic Leukemia Patients In Germany And The Usa In The First Decade Of The Twenty-First Century., 2016 Division of Clinical Epidemiology and Aging Research, German Cancer Research Center (DKFZ); Cardeza Foundation, Division of Hematology, Department of Medicine, Thomas Jefferson University
Trends In Survival Of Chronic Lymphocytic Leukemia Patients In Germany And The Usa In The First Decade Of The Twenty-First Century., Dianne Pulte, Felipe A Castro, Lina Jansen, Sabine Luttmann, Bernd Holleczek, Alice Nennecke, Meike Ressing, Alexander Katalinic, Hermann Brenner
Cardeza Foundation for Hematologic Research
BACKGROUND: Recent population-based studies in the United States of America (USA) and other countries have shown improvements in survival for patients with chronic lymphocytic leukemia (CLL) diagnosed in the early twenty-first century. Here, we examine the survival for patients diagnosed with CLL in Germany in 1997-2011.
METHODS: Data were extracted from 12 cancer registries in Germany and compared to the data from the USA. Period analysis was used to estimate 5- and 10-year relative survival (RS).
RESULTS: Five- and 10-year RS estimates in 2009-2011 of 80.2 and 59.5%, respectively, in Germany and 82.4 and 64.7%, respectively ...
Principles Of Medical Oncology, 2016 University of Massachusetts Medical School
Principles Of Medical Oncology, James Liebmann, Richard S. Pieters
Cancer Concepts: A Guidebook for the Non-Oncologist
Medical oncologists are internists specializing in the care of the adult cancer patient. They frequently function as a primary care physician for patients with cancer. This chapter in Cancer Concepts: A Guidebook for the Non-Oncologist will review the practice of medical oncology and some general principles followed in the use of chemotherapy. Concepts including goals of chemotherapy treatment, evaluation of the effects of chemotherapy, integration of chemotherapy with other cancer treatments, and use of combinations of chemotherapy drugs will be covered.
Novel Oral Transforming Growth Factor-Beta Signaling Inhibitor Ew-7197 Eradicates Cml-Initiating Cells, 2016 Kanazawa University
Novel Oral Transforming Growth Factor-Beta Signaling Inhibitor Ew-7197 Eradicates Cml-Initiating Cells, Kazuhito Naka, Shaoguang Li, Dae-Kee Kim
Open Access Articles
Recent strategies for treating CML patients have focused on investigating new combinations of tyrosine kinase inhibitors (TKIs) as well as identifying novel translational research agents that can eradicate CML leukemia-initiating cells (CML-LICs). However, little is known about the therapeutic benefits such CML-LIC targeting therapies might bring to CML patients. In this study, we investigated the therapeutic potential of EW-7197, an orally bioavailable transforming growth factor-beta signaling inhibitor which has recently been approved as an Investigational New Drug (NIH, USA), to suppress CML-LICs in vivo. Compared to TKI treatment alone, administration of TKI plus EW-7197 to CML-affected mice significantly delayed disease ...
Reduction Of Oxidative Stress And Storage Lesions (Rcsl) In Red Blood Cells - Analysis Of Ascorbic Acid (Aa), N-Acetylcysteine Amide (Ad4), And Serotonin (5-Ht), Shanmuka Gadiraju
Undergraduate Research Posters
Oxidative stress is a common occurrence in red blood cell (RBC) storage in blood banks throughout the world. Typically RBC units stored under routine standard protocol (stored in SAGM-CPD additive solution) can only be kept up to 42 days for transfusion usage before being discarded. I am studying the effects of Ascorbic Acid (AA), N-acetylcysteine amide (AD4), and Serotonin (5-HT) as additives in blood bank storage because I want to find out if these additives can reduce storage-induced oxidative stress on red blood cells (RBCs), in order to help my reader understand how potential blood storage additives can affect the ...
Examination Of Reticulocytosis Among Chronically Transfused Children With Sickle Cell Anemia., 2016 George Washington University
Examination Of Reticulocytosis Among Chronically Transfused Children With Sickle Cell Anemia., Megha Kaushal, Colleen Byrnes, Zarir Khademian, Natalie Duncan, Naomi L.C. Luban, Jeffery L Miller, Ross M. Fasano, Emily Riehm Meier
Pediatrics Faculty Publications
Sickle cell anemia (SCA) is an inherited hemolytic anemia with compensatory reticulocytosis. Recent studies have shown that increased levels of reticulocytosis during infancy are associated with increased hospitalizations for SCA sequelae as well as cerebrovascular pathologies. In this study, absolute reticulocyte counts (ARC) measured prior to transfusion were analysed among a cohort of 29 pediatric SCA patients receiving chronic transfusion therapy (CTT) for primary and secondary stroke prevention. A cross-sectional flow cytometric analysis of the reticulocyte phenotype was also performed. Mean duration of CTT was 3.1 ± 2.6 years. Fifteen subjects with magnetic resonance angiography (MRA) -vasculopathy had significantly ...
Kras Codon 12 And 13 Mutations In Relation To Disease-Freesurvival In Braf-Wild-Type Stage Iii Colon Cancers From An Adjuvant Chemotherapytrial (N0147 Alliance)., Harry H Yoon, David Tougeron, Qian Shi, Steven R Alberts, Michelle R Mahoney, Garth D Nelson, Suresh Nair, Stephen N Thibodeau, Richard M Goldberg, Daniel J Sargent, Frank A Sinicrope
Suresh G. Nair MD
PURPOSE: We examined the prognostic impact of specific KRAS mutations in patients with stage III colon adenocarcinoma receiving adjuvant FOLFOX alone or combined with cetuximab in a phase III trial (N0147). Analysis was restricted to BRAF-wild-type tumors, because BRAF mutation was associated with poor prognosis, and BRAF and KRAS mutations are mutually exclusive.
EXPERIMENTAL DESIGN: The seven most common KRAS mutations in codon 12 and codon 13 were examined in 2,478 BRAF-wild-type tumors. Because KRAS mutations in codon 12 (n = 779) or 13 (n = 220) were not predictive of adjuvant cetuximab benefit, study arms were pooled for analysis. Disease-free ...
Comparison Of Folfiri With Or Without Cetuximab In Patients With Resected Stage Iii Colon Cancer; Ncctg (Alliance) Intergroup Trial N0147, J Huang, Suresh Nair, M Mahoney, G Nelson, A Shields, E Chan, R Goldberg, S Gill, M Kahlenberg, J Quesenberry, S Thibodeau, T Smyrk, A Grothey, F Sinicrope, T Webb, G Farr, B Pockaj, J Berenberg, M Mooney, D Sargent, S Alberts
Suresh G. Nair MD
Background: Two arms with FOLFIRI, with or without cetuximab, were initially included in the randomized phase III intergroup clinical trial NCCTG (North Central Cancer Treatment Group) N0147. When other contemporary trials demonstrated no benefit to using irinotecan as adjuvant therapy, the FOLFIRI-containing arms were discontinued. We report the clinical outcomes for patients randomized to FOLFIRI with or without cetuximab. Patients and Methods: After resection, patients were randomized to 12 biweekly cycles of FOLFIRI, with or without cetuximab. KRAS (Kirsten rat sarcoma viral oncogene homolog) mutation status was retrospectively determined in a central lab. The primary end point was disease-free survival ...
Can Surgeons Improve Survival In Stage Iv Melanoma?, 2015 Lehigh Valley Health Network
Can Surgeons Improve Survival In Stage Iv Melanoma?, Paul Mosca, Erik Teicher, Suresh Nair, Barbara Pockaj
Suresh G. Nair MD
Successful systemic management of stage IV melanoma continues to be elusive because of the paucity of effective therapies. This has fueled the continued interest in surgical resection. Several single-institution studies and a current, large, multi-institutional phase III trial have demonstrated a survival benefit for patients who underwent surgical resection for melanoma metastases. Incorporating these results into new approaches using multimodality treatment may enhance survival in patients with stage IV melanoma.
Hypoxia Induces Phenotypic Plasticity And Therapy Resistance In Melanoma Via The Tyrosine Kinase Receptors Ror1 And Ror2, Michael O'Connell, Katie Marchbank, Marie Webster, Alexander Valiga, Amanpreet Kaur, Adina Vultur, Ling Li, Meenhard Herlyn, Jessie Villanueva, Qin Liu, Xiangfan Yin, Sandy Widura, Janelle Nelson, Nivia Ruiz, Tura C Camilli, Fred E Indig, Keith T Flaherty, Jennifer A Wargo, Dennie T Frederick, Zachary A Cooper, Suresh Nair, Ravi K Amaravadi, Lynn M Schuchter, Giorgos C Karakousis, Wei Xu, Xiaowei Xu, Ashani T Weeraratna
Suresh G. Nair MD
An emerging concept in melanoma biology is that of dynamic, adaptive phenotype switching, where cells switch from a highly proliferative, poorly invasive phenotype to a highly invasive, less proliferative one. This switch may hold significant implications not just for metastasis, but also for therapy resistance. We demonstrate that phenotype switching and subsequent resistance can be guided by changes in expression of receptors involved in the noncanonical Wnt5A signaling pathway, ROR1 and ROR2. ROR1 and ROR2 are inversely expressed in melanomas and negatively regulate each other. Furthermore, hypoxia initiates a shift of ROR1-positive melanomas to a more invasive, ROR2-positive phenotype. Notably ...
Patient And Tumor Characteristics And Braf And Kras Mutations In Coloncancer, Ncctg/Alliance N0147., 2015 Division of Hematology/Division of Medical Oncology
Patient And Tumor Characteristics And Braf And Kras Mutations In Coloncancer, Ncctg/Alliance N0147., Wilson I Gonsalves, Michelle R Mahoney, Daniel J Sargent, Garth D Nelson, Steven R Alberts, Frank A Sinicrope, Richard M Goldberg, Paul J Limburg, Stephen N Thibodeau, Axel Grothey, Joleen M Hubbard, Emily Chan, Suresh Nair, Jeffrey L Berenberg, Robert R Mcwilliams
Suresh G. Nair MD
BACKGROUND: KRAS and BRAF (V600E) mutations are important predictive and prognostic markers, respectively, in colon cancer, but little is known about patient and clinical factors associated with them.
METHODS: Two thousand three hundred twenty-six of 3397 patients in the N0147 phase III adjuvant trial for stage III colon cancer completed a patient questionnaire. Primary tumors were assessed for KRAS and BRAF (V600E) mutations and defective mismatch repair (dMMR) status. Logistic regression models and categorical data analysis were used to identify associations of patient and tumor characteristics with mutation status. All statistical tests were two-sided.
RESULTS: KRAS (35%) and BRAF (V600E ...
Homozygous Knockout Of The Piezo1 Gene In The Zebrafish Is Not Associated With Anemia, 2015 Harvard Medical School
Homozygous Knockout Of The Piezo1 Gene In The Zebrafish Is Not Associated With Anemia, Boris E. Shmukler, Nicholas C. Huston, Jonathan N. Thon, Chih-Wen Ni, George Kourkoulis, Nathan D. Lawson, Barry H. Paw, Seth L. Alper
Molecular, Cell and Cancer Biology Publications
We have now examined the erythroid phenotype in this zebrafish strain carrying a ZFN genomic knockout of piezo1. Genotyping was performed as previously described. In contrast to the anemic phenotype observed in zebrafish subjected to morpholino knockdown of piezo, the genomic ZFN knockout of piezo1 did not segregate either with anemia in the 3-dpf embryo or with dysmorphic erythrocyte morphology in the adult fish.
Interview With Scott Hamilton Goodnight, Jr., M.D., 2015 Oregon Health & Science University
Interview With Scott Hamilton Goodnight, Jr., M.D., Scott Hamilton Goodnight, Jr., M.D. (Interviewee), Grover C. Bagby, M.D. (Interviewer)
Oral History Collection
In this oral history interview, Scott Goodnight, Jr., M.D., reflects on his life and career in medicine. He begins the interview by discussing his father’s medical school career and practice in downtown Portland and Goodnight’s childhood growing up in Northwest Portland. He goes on to describe his undergraduate and graduate education in medicine at the University of Oregon, meeting his wife Cecelia, and his early involvement in biomedical research. Drafted by the Army, Goodnight also discusses his time spent at the Fitzsimmons General Hospital and General Medicine Clinic as well as the 85th Evacuation Hospital in ...