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T-Lymphocyte Responses To Intestinally Absorbed Antigens Can Contribute To Adipose Tissue Inflammation And Glucose Intolerance During High Fat Feeding, Yuehui Wang, Jianing Li, Lihua Tang, Yu Wang, Richard Charnigo, Willem de Villiers, Erik Eckhardt 2010 University of Kentucky

T-Lymphocyte Responses To Intestinally Absorbed Antigens Can Contribute To Adipose Tissue Inflammation And Glucose Intolerance During High Fat Feeding, Yuehui Wang, Jianing Li, Lihua Tang, Yu Wang, Richard Charnigo, Willem De Villiers, Erik Eckhardt

Internal Medicine Faculty Publications

BACKGROUND: Obesity is associated with inflammation of visceral adipose tissues, which increases the risk for insulin resistance. Animal models suggest that T-lymphocyte infiltration is an important early step, although it is unclear why these cells are attracted. We have recently demonstrated that dietary triglycerides, major components of high fat diets, promote intestinal absorption of a protein antigen (ovalbumin, "OVA"). The antigen was partly transported on chylomicrons, which are prominently cleared in adipose tissues. We hypothesized that intestinally absorbed gut antigens may cause T-lymphocyte associated inflammation in adipose tissue.

METHODOLOGY/PRINCIPAL FINDINGS: Triglyceride absorption promoted intestinal absorption of OVA into adipose ...


Mcc-555-Induced Nag-1 Expression Is Mediated In Part By Klf4., Maria Cekanova, Seong-Ho Lee, Michael McEntee, Seung Baek 2010 The University of Tennessee

Mcc-555-Induced Nag-1 Expression Is Mediated In Part By Klf4., Maria Cekanova, Seong-Ho Lee, Michael Mcentee, Seung Baek

Maria Cekanova MS, RNDr, PhD

Peroxisome proliferator-activated receptor gamma (PPARgamma) plays a central role in cell differentiation, metabolism and tumorigenesis. We have investigated the therapeutic properties of 5-[[6-[(2-fluorophenyl)-methoxy]-2-napthalenyl]-methyl]-2,4-thiazolidinedione (MCC-555) a PPARgamma agonist in human colorectal cancer cells. To elucidate the molecular mechanism(s), by which MCC-555 exerts its effects on the human colorectal cancer cells, we have analyzed the expression of two pro-apoptotic proteins, Krüppel-like factor 4 (KLF4) and nonsteroidal anti-inflammatory drug (NSAID)-activated gene-1 (NAG-1). MCC-555-induced expression of the transcription factor, KLF-4 was blocked by a PPARgamma specific antagonist GW9662 in PPARgamma-dependent manner in HCT-116 cells. We ...


Multiple Mechanisms Are Involved In 6-Gingerol-Induced Cell Growth Arrest And Apoptosis In Human Colorectal Cancer Cells, Seong Lee, Maria Cekanova, Seung Baek 2010 University of Tennessee - Knoxville

Multiple Mechanisms Are Involved In 6-Gingerol-Induced Cell Growth Arrest And Apoptosis In Human Colorectal Cancer Cells, Seong Lee, Maria Cekanova, Seung Baek

Maria Cekanova MS, RNDr, PhD

6-Gingerol, a natural product of ginger, has been known to possess anti-tumorigenic and pro-apoptotic activities. However, the mechanisms by which it prevents cancer are not well understood in human colorectal cancer. Cyclin D1 is a proto-oncogene that is overexpressed in many cancers and plays a role in cell proliferation through activation by beta-catenin signaling. Nonsteroidal anti-inflammatory drug (NSAID)-activated gene-1 (NAG-1) is a cytokine associated with pro-apoptotic and anti-tumorigenic properties. In the present study, we examined whether 6-gingerol influences cyclin D1 and NAG-1 expression and determined the mechanisms by which 6-gingerol affects the growth of human colorectal cancer cells in ...


Peroxisome Proliferator-Activated Receptor Ligand Mcc-555 Suppresses Intestinal Polyps In Apcmin/+ Mice Via Extracellular Signal-Regulated Kinase And Peroxisome Proliferator-Activated Receptor-Dependent Pathways, K Yamaguchi, Maria Cekanova, Michael McEntee, J Yoon, S Fischer, I Renes, I Van Seungnigen, Seung Baek 2010 University of Tennessee - Knoxville

Peroxisome Proliferator-Activated Receptor Ligand Mcc-555 Suppresses Intestinal Polyps In Apcmin/+ Mice Via Extracellular Signal-Regulated Kinase And Peroxisome Proliferator-Activated Receptor-Dependent Pathways, K Yamaguchi, Maria Cekanova, Michael Mcentee, J Yoon, S Fischer, I Renes, I Van Seungnigen, Seung Baek

Maria Cekanova MS, RNDr, PhD

A large body of studies has suggested that peroxisome proliferator-activated receptor gamma (PPARgamma) ligands, such as thiazolidinedione, are potent candidates for chemopreventive agents. MCC-555 is a PPARgamma/alpha dual agonist and has been shown previously to induce apoptosis in vitro; however, the molecular mechanisms by which MCC-555 affects antitumorigenesis in vivo are poorly understood. In this study, we explored the antitumorigenic effects of MCC-555 both in cell culture and in Apc-deficient mice, an animal model for human familial adenomatous polyposis. MCC-555 increased MUC2 expression in colorectal and lung cancer cells, and treatment with the PPARgamma antagonist GW9662 revealed that MUC2 ...


Impact Of A Comprehensive Nutrition And Lifestyle Education Intervention On Body Weight And Health-Related Outcomes In Morbidly-Obese Hispanic-Americans Following Laparoscopic Roux-En Y Gastric Bypass, Monica Petasne Nijamkin 2010 Florida International University

Impact Of A Comprehensive Nutrition And Lifestyle Education Intervention On Body Weight And Health-Related Outcomes In Morbidly-Obese Hispanic-Americans Following Laparoscopic Roux-En Y Gastric Bypass, Monica Petasne Nijamkin

FIU Electronic Theses and Dissertations

As morbid obesity increasingly affects Hispanic-Americans, the incidence of Roux-en-Y gastric bypass procedures (RYGB) among this population rises. Prospective research on the impact of postoperative educational interventions focused on Hispanic-Americans is needed to prevent premature weight loss plateau, weight regain, nutritional deficiencies, and relapse of obesity-related comorbidities. This randomized-controlled study evaluated the impact of a comprehensive nutrition and lifestyle education intervention (6 biweekly postoperative sessions that incorporated motivational strategies for behavioral change) as compared to a non-comprehensive approach (printed guidelines for healthy lifestyle). The variables to consider are body weight, obesity-related comorbidities (depression, diabetes, dyslipidemia, and others), nutrient status, physical ...


Epicatechin Gallate Suppresses Oxidative Stress-Induced Muc5ac Overexpression By Interaction With Epidermal Growth Factor Receptor, HJ Kim, JH Ryu, CH Kim, JW Lim, UY Moon, GH Lee, Seung Baek, JH Yoon 2010 The University of Tennessee

Epicatechin Gallate Suppresses Oxidative Stress-Induced Muc5ac Overexpression By Interaction With Epidermal Growth Factor Receptor, Hj Kim, Jh Ryu, Ch Kim, Jw Lim, Uy Moon, Gh Lee, Seung Baek, Jh Yoon

Seung J Baek

The goal of this study was to investigate the effect of epicatechin gallate (ECG), a component of green tea polyphenols, on the signal pathway for oxidative stress-induced intracellular reactive oxygen species (ROS) generation and MUC5AC overexpression in normal human nasal epithelial (NHNE) cells. Passage-2 NHNE cells were used, and ECG was administered before stimulation with exogenous hydrogen peroxide (H(2)O(2)). MUC5AC gene and protein levels were measured by real-time PCR and dot blot analysis. Western blot analysis and immunocytofluorescence study were performed for detecting the activity of epidermal growth factor receptor (EGFR). Exogenous H(2)O(2) increases ...


Activating Transcription Factor 2 (Atf2) Controls Tolfenamic Acid-Induced Atf3 Expression Via Map Kinase Pathways., Seong-Ho Lee, Jae Hoon Bahn, Nichelle Whitlock, Seung Baek 2010 The University of Tennessee

Activating Transcription Factor 2 (Atf2) Controls Tolfenamic Acid-Induced Atf3 Expression Via Map Kinase Pathways., Seong-Ho Lee, Jae Hoon Bahn, Nichelle Whitlock, Seung Baek

Seung J Baek

Tolfenamic acid (TA) is a non-steroidal anti-inflammatory drug associated with anti-tumorigenic and pro-apoptotic properties in animal and in vitro models of cancer. However, the underlying cellular mechanisms by which TA exerts its effects are only partially understood. Activating transcription factor 3 (ATF3) is a member of the ATF/CREB subfamily of the basic region-leucine zipper family and has been known as a tumor suppressor in human colorectal cancer cells. The present study was performed to observe whether ATF3 mediates TA-induced apoptosis and to elucidate the molecular mechanism of ATF3 transcription induced by TA. TA treatment and ectopic expression of ATF3 ...


Autonomous Bioluminescent Expression Of The Bacterial Luciferase Gene Cassette (Lux) In A Mammalian Cell Line, Dan Close, Patterson, Ripp, Seung Baek, Sanseverino, Gary Sayler 2010 The University of Tennessee

Autonomous Bioluminescent Expression Of The Bacterial Luciferase Gene Cassette (Lux) In A Mammalian Cell Line, Dan Close, Patterson, Ripp, Seung Baek, Sanseverino, Gary Sayler

Seung J Baek

No abstract provided.


(-)-Epigallocatechin-3-Gallate (Egcg) Post-Transcriptionally And Post-Translationally Suppresses The Cell Proliferative Protein Trop2 In Human Colorectal Cancer Cells., Seung Baek, Mugdha Sukhthankar, SAVERIO Alberti 2010 The University of Tennessee

(-)-Epigallocatechin-3-Gallate (Egcg) Post-Transcriptionally And Post-Translationally Suppresses The Cell Proliferative Protein Trop2 In Human Colorectal Cancer Cells., Seung Baek, Mugdha Sukhthankar, Saverio Alberti

Seung J Baek

BACKGROUND: TROP-2 is a tumor-promoting molecule that has been found to be overexpressed in many cancer cells, making it a plausible biomarker of carcinogenesis. The main aim of this study was to examine the effect of green tea catechins (namely, (-)-epigallocatechin-3-gallate; EGCG) on TROP-2 expression. MATERIALS AND METHODS: Western blot and RT-PCR were applied to assess TROP2 expression in colorectal cancer cells and tissues. RESULTS: Two different mechanisms were found to operate in diverse cell lines. In SW480 cells, EGCG affected the post-transcriptional processing of the TROP-2 mRNA, as this was quickly and specifically degraded in the presence of EGCG ...


Mcc-555-Induced Nag-1 Expression Is Mediated In Part By Klf4., Maria Cekanova, Seong-Ho Lee, Michael McEntee, Seung Baek 2010 The University of Tennessee

Mcc-555-Induced Nag-1 Expression Is Mediated In Part By Klf4., Maria Cekanova, Seong-Ho Lee, Michael Mcentee, Seung Baek

Seong-Ho Lee

Peroxisome proliferator-activated receptor gamma (PPARgamma) plays a central role in cell differentiation, metabolism and tumorigenesis. We have investigated the therapeutic properties of 5-[[6-[(2-fluorophenyl)-methoxy]-2-napthalenyl]-methyl]-2,4-thiazolidinedione (MCC-555) a PPARgamma agonist in human colorectal cancer cells. To elucidate the molecular mechanism(s), by which MCC-555 exerts its effects on the human colorectal cancer cells, we have analyzed the expression of two pro-apoptotic proteins, Krüppel-like factor 4 (KLF4) and nonsteroidal anti-inflammatory drug (NSAID)-activated gene-1 (NAG-1). MCC-555-induced expression of the transcription factor, KLF-4 was blocked by a PPARgamma specific antagonist GW9662 in PPARgamma-dependent manner in HCT-116 cells. We ...


Mcc-555-Induced Nag-1 Expression Is Mediated In Part By Klf4., Maria Cekanova, Seong-Ho Lee, Michael McEntee, Seung Baek 2010 The University of Tennessee

Mcc-555-Induced Nag-1 Expression Is Mediated In Part By Klf4., Maria Cekanova, Seong-Ho Lee, Michael Mcentee, Seung Baek

Seung J Baek

Peroxisome proliferator-activated receptor gamma (PPARgamma) plays a central role in cell differentiation, metabolism and tumorigenesis. We have investigated the therapeutic properties of 5-[[6-[(2-fluorophenyl)-methoxy]-2-napthalenyl]-methyl]-2,4-thiazolidinedione (MCC-555) a PPARgamma agonist in human colorectal cancer cells. To elucidate the molecular mechanism(s), by which MCC-555 exerts its effects on the human colorectal cancer cells, we have analyzed the expression of two pro-apoptotic proteins, Krüppel-like factor 4 (KLF4) and nonsteroidal anti-inflammatory drug (NSAID)-activated gene-1 (NAG-1). MCC-555-induced expression of the transcription factor, KLF-4 was blocked by a PPARgamma specific antagonist GW9662 in PPARgamma-dependent manner in HCT-116 cells. We ...


Effects Of Atmospheric Non-Thermal Plasma On Invasion Of Colorectal Cancer Cells., Seung Baek, Chul-Ho Kim, S Kwon, K Lee, S Jun, Philip Rack 2010 The University of Tennessee

Effects Of Atmospheric Non-Thermal Plasma On Invasion Of Colorectal Cancer Cells., Seung Baek, Chul-Ho Kim, S Kwon, K Lee, S Jun, Philip Rack

Seung J Baek

No abstract provided.


Rbc And Wbc Fatty Acid Composition Following Consumption Of An Omega 3 Supplement: Lessons For Future Clinical Trials, Theodore R. Witte, Alexander J. Salazar, Oscar F. Ballester, W. Elaine Hardman 2010 Marshall University

Rbc And Wbc Fatty Acid Composition Following Consumption Of An Omega 3 Supplement: Lessons For Future Clinical Trials, Theodore R. Witte, Alexander J. Salazar, Oscar F. Ballester, W. Elaine Hardman

Biochemistry and Microbiology

Background: Results from increasing numbers of in vitro and in vivo studies have demonstrated that omega 3 fatty acids incorporated in cell culture media or in the diet of the animals can suppress the growth of cancers. When human clinical trials are initiated to determine the ability of omega 3 fatty acids to alter growth or response to chemotherapeutic interventions of cancers, it will be essential to determine the omega 3 intake of individuals in the trial to determine compliance with consumption of the supplement and to correlate with endpoints of efficacy. We wondered if the fatty acid composition of ...


4 Part Research Project: Cholecystokinin, Marcie Tasker 2010 Johnson & Wales University - Providence

4 Part Research Project: Cholecystokinin, Marcie Tasker

Academic Symposium of Undergraduate Scholarship

This 4 part research paper contains information on the biological molecule Cholecystokinin. The following research discusses and presents evidence of the name, history and structure of the hormone. It also discusses the different chemical reactions Cholecystokinin has in the body, the biological roles of Cholecystokinin and the importance of Cholecystokinin focusing specifically on the unexplained obesity issue of our world.


Algal Biofuel, Feb. 12, 2010, Harry Reid Center, University of Nevada, Las Vegas 2010 Harry Reid Center, University of Nevada, Las Vegas

Algal Biofuel, Feb. 12, 2010, Harry Reid Center, University Of Nevada, Las Vegas

Biofuels

News in Brief

1. Fatty Acids Protect Against Aging (Feb. 8, 2010)


Cell Adhesion Property Affected By Cyclooxygenase And Lipoxygenase: Opto-Electric Approach (With Chang Kyoung Choi, Mugdha Sukhthankar, Chul-Ho Kim, Seong-Ho Lee, Anthony English, Kenneth D. Kihm., Seung Baek 2009 The University of Tennessee

Cell Adhesion Property Affected By Cyclooxygenase And Lipoxygenase: Opto-Electric Approach (With Chang Kyoung Choi, Mugdha Sukhthankar, Chul-Ho Kim, Seong-Ho Lee, Anthony English, Kenneth D. Kihm., Seung Baek

Seung J Baek

Expression of cyclooxygenases (COX) and lipoxygenases (LOX) has been linked to many pathophysiological phenotypes, including cell adhesion. However, many current approaches to measure cellular changes are performed only in a fixed-time point. Since cells dynamically move in conjunction with the cell matrix, there is a pressing need for dynamic or time-dependent methods for the investigation of cell properties. In the presented study, we used stable human colorectal cancer cell lines ectopically expressing COX-1, COX-2, and 15LOX-1, to investigate whether expression of COX-1, COX-2, or 15LOX-1 would affect cell adhesion using our opto-electric methodology. In a fixed-time point experiment, only COX-1- ...


Nsaid-Activated Gene-1 As A Molecular Target For Capsaicin-Induced Apoptosis Through A Novel Molecular Mechanism Involving Gsk3beta, C/Ebpbeta And Atf3, Seung Baek, Seong-Ho Lee, Chutwadee Krisanapun 2009 The University of Tennessee

Nsaid-Activated Gene-1 As A Molecular Target For Capsaicin-Induced Apoptosis Through A Novel Molecular Mechanism Involving Gsk3beta, C/Ebpbeta And Atf3, Seung Baek, Seong-Ho Lee, Chutwadee Krisanapun

Seung J Baek

Capsaicin, a natural product of the Capsicum species of red peppers, is known to induce apoptosis and suppress growth. Non-steroidal anti-inflammatory drug-activated gene-1 (NAG-1) is a cytokine associated with pro-apoptotic and antitumorigenic property in colorectal and lung cancer. Our data demonstrate that capsaicin leads to induction of apoptosis and up-regulates NAG-1 gene expression at the transcriptional level. Overexpression of CCAAT/enhancer binding protein beta (C/EBPbeta) caused a significant increase of basal and capsaicin-induced NAG-1 promoter activity. We subsequently identified C/EBPbeta binding sites in the NAG-1 promoter responsible for capsaicin-induced NAG-1 transactivation. Electrophoretic mobility shift assay and chromatin immunoprecipitation ...


A Potential Proliferative Gene, Nudt6, Is Down-Regulated By Green Tea Catechins At The Posttranscriptional Level, Seung Baek, Mugdha Sukthankar, CK Choi, A English, JS Kim 2009 The University of Tennessee

A Potential Proliferative Gene, Nudt6, Is Down-Regulated By Green Tea Catechins At The Posttranscriptional Level, Seung Baek, Mugdha Sukthankar, Ck Choi, A English, Js Kim

Seung J Baek

The main aims of this study were to elucidate the effect of green tea catechins on Nudix-type motif 6 (NUDT6) suppression and to characterize NUDT6's biological activity. Our microarray data showed that the green tea component epicatechin-3-gallate suppressed NUDT6 expression, and this was confirmed by RT-PCR. Subsequently, the use of different catechins showed that the effect of epigallocatechin-3-gallate (EGCG) was stronger than that of other catechins. At the posttranscriptional level, EGCG decreased the RNA stability of NUDT6, indicating it as a potential mechanism of NUDT6 suppression. Further cloning of the 3' untranslated region of human NUDT6 mRNA resulted in ...


In Vitro Anti-Proliferative Activity Of Alcoholic Stem Extract Of Coscinium Fenestratum In Human Colorectal Cancer Cells, P Rojsanga, M Sukthankar, C Krisanapun, W Gritsanapan, D Lawson, Seung Baek 2009 The University of Tennessee

In Vitro Anti-Proliferative Activity Of Alcoholic Stem Extract Of Coscinium Fenestratum In Human Colorectal Cancer Cells, P Rojsanga, M Sukthankar, C Krisanapun, W Gritsanapan, D Lawson, Seung Baek

Seung J Baek

Coscinium fenestratum (Gaertn.) Colebr. is traditionally used for the treatment of cancer, arthritis and diabetes mellitus. The purpose of this study was to determine the molecular mechanisms by which this plant shows beneficial effects. An 80% ethanolic extract of C. fenestratum (80ET) was separated by its polarity into dichloromethane (DCM) and aqueous fractions (WF), and the anti-proliferative effects of 80ET, DCM and WF were investigated. Berberine, one of the major components of C. fenestratum, was used as a control. The 80ET, DCM, WF and berberine showed anti-proliferative activity as assessed by cell growth assay. Subsequently, the pro-apoptotic proteins NAG-1 and ...


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