Spatiotemporal Effects Of A Controlled-Release Anti-Inflammatory Drug On The Cellular Dynamics Of Host Response,
2011
Massachusetts Institute of Technology
Spatiotemporal Effects Of A Controlled-Release Anti-Inflammatory Drug On The Cellular Dynamics Of Host Response, Tram T. Dang, Kaitlin M. Bratlie, Said R. Bogatyrev, Xiao Chen, Robert Langer, Daniel G. Anderson
Kaitlin M. Bratlie
In general, biomaterials induce a non-specific host response when implanted in the body. This reaction has the potential to interfere with the function of the implanted materials. One method for controlling the host response is through local, controlled-release of anti-inflammatory agents. Herein, we investigate the spatial and temporal effects of an anti-inflammatory drug on the cellular dynamics of the innate immune response to subcutaneously implanted poly(lactic-co-glycolic) microparticles. Noninvasive fluorescence imaging was used to investigate the influence of dexamethasone drug loading and release kinetics on the local and systemic inhibition of inflammatory cellular activities. Temporal monitoring of host response showed that …
Automated Sequence- And Stereo-Specific Assignment Of Methyl-Labeled Proteins By Paramagnetic Relaxation And Methyl–Methyl Nuclear Overhauser Enhancement Spectroscopy,
2011
National Institutes of Health
Automated Sequence- And Stereo-Specific Assignment Of Methyl-Labeled Proteins By Paramagnetic Relaxation And Methyl–Methyl Nuclear Overhauser Enhancement Spectroscopy, Vincenzo Venditti, Nicolas L. Fawzi, G. Marius Clore
Vincenzo Venditti
Methyl-transverse relaxation optimized spectroscopy is rapidly becoming the preferred NMR technique for probing structure and dynamics of very large proteins up to ~1 MDa in molecular size. Data interpretation, however, necessitates assignment of methyl groups which still presents a very challenging and time-consuming process. Here we demonstrate that, in combination with a known 3D structure, paramagnetic relaxation enhancement (PRE), induced by nitroxide spin-labels incorporated at only a few surface-exposed engineered cysteines, provides fast, straightforward and robust access to methyl group resonance assignments, including stereoassignments for the methyl groups of leucine and valine. Neither prior assignments, including backbone assignments, for the …
A Structurally Driven Analysis Of Thiol Reactivity In Mammalian Albumins,
2011
University of Siena
A Structurally Driven Analysis Of Thiol Reactivity In Mammalian Albumins, Ottavia Spiga, Domenico Summa, Simone Cirri, Andrea Bernini, Vincenzo Venditti, Matteo De Chiara, Raffaella Priora, Simona Frosail, Antonios Margaritis, Danila Di Giuseppe, Paolo Di Simplicio, Neri Niccolai
Vincenzo Venditti
Understanding the structural basis of protein redox activity is still an open question. Hence, by using a structural genomics approach, different albumins have been chosen to correlate protein structural features with the corresponding reaction rates of thiol exchange between albumin and disulfide DTNB. Predicted structures of rat, porcine, and bovine albumins have been compared with the experimentally derived human albumin. High structural similarity among these four albumins can be observed, in spite of their markedly different reactivity with DTNB. Sequence alignments offered preliminary hints on the contributions of sequence-specific local environments modulating albumin reactivity. Molecular dynamics simulations performed on experimental …
Real-Time In Vivo Detection Of Biomaterial-Induced Reactive Oxygen Species,
2011
Massachusetts Institute of Technology
Real-Time In Vivo Detection Of Biomaterial-Induced Reactive Oxygen Species, Minglin Ma, Wendy F. Liu, Kaitlin M. Bratlie, Tram Dang, Robert Langer, Daniel G. Anderson
Kaitlin M. Bratlie
The non-specific host response to implanted biomaterials is often a key challenge of medical device design. To evaluate biocompatibility, measuring the release of reactive oxygen species (ROS) produced by inflammatory cells in response to biomaterial surfaces is a well-established method. However, the detection of ROS in response to materials implanted in vivo has not yet been demonstrated. Here, we develop a bioluminescence whole animal imaging approach to observe ROS released in response to subcutaneously-implanted materials in live animals. We compared the real-time generation of ROS in response to two representative materials, polystyrene and alginate, over the course of 28 days. …
The Role Of Ghrelin And Leptin In Obesity: Is Exogenous Administration Of These Hormones A Possible Drug Therapy?,
2011
Touro College
The Role Of Ghrelin And Leptin In Obesity: Is Exogenous Administration Of These Hormones A Possible Drug Therapy?, Peri Eckstein
The Science Journal of the Lander College of Arts and Sciences
Ghrelin and leptin are two hormones that have been recognized to have a major influence on energy balance. Leptin is a mediator of long term regulation of energy balance, suppressing food intake and thereby inducing weight loss. Ghrelin, on the other hand, is a fast acting hormone, playing a role in meal initiation. As a growing number of people suffer from obesity, understanding the mechanisms by which various hormones and neurotransmitters influence energy balance has been a subject of intense research. This paper provides background on leptin and ghrelin hormones, their role in food intake and body weight in humans, …
The Effect Of Melatonin On The Ovaries,
2011
Touro College
The Effect Of Melatonin On The Ovaries, Jaclyn Starr
The Science Journal of the Lander College of Arts and Sciences
Melatonin is a very small molecule whose effects can be both detrimental and beneficial to the ovaries depending on its concentration. Too much of anything is usually not good and so is the case with melatonin. Very high doses can be damaging, but in the right amount melatonin may be able to combat various diseases and increase the chances for fertility in women.
The Effectiveness And Safety Of Antibiotic Treatment In Healing Tympanic Membrane Perforation In Aboriginal Children,
2011
Philadelphia College of Osteopathic Medicine
The Effectiveness And Safety Of Antibiotic Treatment In Healing Tympanic Membrane Perforation In Aboriginal Children, Shannon M. Rice
PCOM Physician Assistant Studies Student Scholarship
The objective of this systematic review is to determine whether or not antibiotic treatment is safe and effective in healing tympanic membrane (TM) perforation in Aboriginal children diagnosed with chronic suppurative otitis media and otitis media with effusion less than 16 years of age.
How Safe And Effective Is Infliximab In The Treatment Of Children With Moderate To Severe Crohn’S Disease?,
2011
Philadelphia College of Osteopathic Medicine
How Safe And Effective Is Infliximab In The Treatment Of Children With Moderate To Severe Crohn’S Disease?, Jackie Linh
PCOM Physician Assistant Studies Student Scholarship
Objective: The objective of this systematic review is to determine whether or not “how safe and effective is infliximab in treatment of children with moderate to severe Crohn’s disease?”
What Is The Safety And Efficacy Of Tarenflurbil (R-Flurbiprofen) In Mild To Moderate Alzheimer’S Disease?,
2011
Philadelphia College of Osteopathic Medicine
What Is The Safety And Efficacy Of Tarenflurbil (R-Flurbiprofen) In Mild To Moderate Alzheimer’S Disease?, Lauren Viscount
PCOM Physician Assistant Studies Student Scholarship
OBJECTIVE: The objective of this systematic review is to determine what is the safety and efficacy of Tarenflurbil (R-flubiprofen) in mild to moderate Alzheimer’s Disease.
Is Alitretinoin (9-Cis-Retinoic Acid) Safe And Effective For Use In Hand Eczema?,
2011
Philadelphia College of Osteopathic Medicine
Is Alitretinoin (9-Cis-Retinoic Acid) Safe And Effective For Use In Hand Eczema?, Jeanne K. Seeberger
PCOM Physician Assistant Studies Student Scholarship
OBJECTIVE: To determine if Alitretinoin is a safe and effective alternative to current treatments in reducing the symptoms of Hand Eczema (HE)
Does Fish Oil Supplementation (Omega-3 Pufa) Cause Mood Improvement In Adults With Depression,
2011
Philadelphia College of Osteopathic Medicine
Does Fish Oil Supplementation (Omega-3 Pufa) Cause Mood Improvement In Adults With Depression, Lauren Cabana
PCOM Physician Assistant Studies Student Scholarship
Objective: The objective of this systematic review is to determine whether or not “Does fish oil supplementation (omega-3 PUFA) cause mood improvement in adults with depression?”
Chemical Composition And Product Quality Control Of Turmeric (Curcuma Longa L.),
2011
Stephen F Austin State University, Arthur Temple College of Forestry and Agriculture
Chemical Composition And Product Quality Control Of Turmeric (Curcuma Longa L.), Shiyou Li, Wei Yuan, Guangrui Deng, Ping Wang, Peiying Yang, Bharat Aggarwal
Faculty Publications
Chemical constituents of various tissues of turmeric (Curcuma longa L.) have been extensively investigated. To date, at least 235 compounds, primarily phenolic compounds and terpenoids have been identified from the species, including 22 diarylheptanoids and diarylpentanoids, eight phenylpropene and other phenolic compounds, 68 monoterpenes, 109 sesquiterpenes, five diterpenes, three triterpenoids, four sterols, two alkaloids, and 14 other compounds. Curcuminoids (diarylheptanoids) and essential oils are major bioactive ingredients showing various bioactivities in in vitro and in vivo bioassays. Curcuminoids in turmeric are primarily accumulated in rhizomes. The essential oils from leaves and flowers are usually dominated by monoterpenes while those from …
Pharmacological Chaperoning In Fabry Disease,
2011
University of Massachusetts Amherst
Pharmacological Chaperoning In Fabry Disease, Jerome Rogich
Masters Theses 1911 - February 2014
Fabry Disease is an X-‐linked lysosomal storage disorder characterized by a variety of symptoms including hypohydrosis, seizures, cardiac abnormalities, skin lesions, and chronic pain. These symptoms stem from a lack of functional endogenous α-‐ Galactosidase A (α-GAL), which leads to an accrual of its natural substrate. The severity of the disease symptoms can be directly correlated with the amount of residual enzyme activity. It has been shown that an imino sugar, 1-deoxygalactonojirimycin (DGJ), can increase enzymatic activity and clear excess substrate. This pH-‐dependent chaperoning phenomenon is believed to arise from the presence of aspartic acid 170 in the active site. …
Antispasmodic And Ca++ Antagonist Potential Of Marrubiin, A Labdane Type Diterpene From Phlomis Bracteosa,
2011
Kohat University of Science and Technology, Kohat, Pakistan
Antispasmodic And Ca++ Antagonist Potential Of Marrubiin, A Labdane Type Diterpene From Phlomis Bracteosa, Javid Hussain, Riaz Ullah, Arif-Ullah Khan, Fazal Mabood, Mohammad Raza Shah, Ahmed Al-Harrasi, Anwar Gilani
Department of Biological & Biomedical Sciences
A tricyclic labdane type diterpene was isolated for the first time from ethyl acetate soluble part of Phlomis bracteosa. Its structure was confirmed by x-ray which was found to be marrubiin. When studied in isolated rabbit jejunum, marrubiin caused concentration-dependent relaxation of spontaneous and high K+ (80 mM)-induced contractions, like that caused by verapamil, indicating that marrubiin exhibits spasmolytic activity, possibly mediated through Ca++ channel blocking action.
Does Melatonin Have A Significantly Positive Effect On Night-Shift Workers' Overall Recovery, Mood, Tiredness, Alertness, Attention, And Sleep Quality?,
2011
Philadelphia College of Osteopathic Medicine
Does Melatonin Have A Significantly Positive Effect On Night-Shift Workers' Overall Recovery, Mood, Tiredness, Alertness, Attention, And Sleep Quality?, Vincy Zachariah
PCOM Physician Assistant Studies Student Scholarship
OBJECTIVE: The objective of this systematic review is to verify whether or not Melatonin has a significantly positive effect on night -shift workers' overall recovery, mood, tiredness. alertness, attention. and sleep quality.
Is Maternal Buprenorphine Use During Pregnancy Effective In Preventing Neonatal Abstinence Syndrome?,
2011
Philadelphia College of Osteopathic Medicine
Is Maternal Buprenorphine Use During Pregnancy Effective In Preventing Neonatal Abstinence Syndrome?, Danielle L. Parrott
PCOM Physician Assistant Studies Student Scholarship
OBJECTIVE: The objective of this systematic review is to determine whether or not maternal buprenorphine use during pregnancy is effective in preventing neonatal abstinence syndrome (NAS).
Is Opt-80 A Safe And Effective Treatment For Patients With C.Difficile Infection?,
2011
Philadelphia College of Osteopathic Medicine
Is Opt-80 A Safe And Effective Treatment For Patients With C.Difficile Infection?, Heather A. Shupp
PCOM Physician Assistant Studies Student Scholarship
OBJECTIVE: Objective of this systematic review is to determine whether or not OPT-80 is a safe and effective treatment for patients with C.difficile infection.
Is Actemra (Tocilizumab) In Combination With Methotrexate An Effective Treatment For Rheumatoid Arthritis In Patients Who Had An Inadequate Response To Methotrexate Alone?,
2011
Philadelphia College of Osteopathic Medicine
Is Actemra (Tocilizumab) In Combination With Methotrexate An Effective Treatment For Rheumatoid Arthritis In Patients Who Had An Inadequate Response To Methotrexate Alone?, Jennifer Nagle
PCOM Physician Assistant Studies Student Scholarship
OBJECTIVE: The objective of this systematic review is to determine whether or not Actemra (tocilizumab) in combination with methotrexate is an effective treatment for rheumatoid arthritis in patients who had an inadequate response to methotrexate alone. Previous studies have shown other biologic agents and disease modifying antirheumatic drugs (DMARDs) to have some efficacy in treating rheumatoid arthritis. However, tocilizumab is a newly approved FDA drug and therefore, its efficacy in treating RA has yet to be determined.
Does Venlafaxine (Effexor) Decrease The Frequency Of Hot Flashes In Women With Breast Cancer?,
2011
Philadelphia College of Osteopathic Medicine
Does Venlafaxine (Effexor) Decrease The Frequency Of Hot Flashes In Women With Breast Cancer?, Allison Berneking
PCOM Physician Assistant Studies Student Scholarship
Objective: The objective of this systematic review is to determine whether or not “Does venlafaxine (Effexor) decrease the frequency of hot flashes in women with breast cancer?”
Are Proton Pump Inhibitors A Safe And Effective Treatment For Gastroesophageal Reflux Disease In Infants Less Than Twelve Months Old?,
2011
Philadelphia College of Osteopathic Medicine
Are Proton Pump Inhibitors A Safe And Effective Treatment For Gastroesophageal Reflux Disease In Infants Less Than Twelve Months Old?, Mariah Smith
PCOM Physician Assistant Studies Student Scholarship
OBJECTIVE: The objective of this systematic review is to determine whether or not proton pump inhibitors are a safe and effective treatment for gastroesophageal reflux disease in infants less than twelve months old.
